المؤلفون: Butin, M., Martins-Simões, P., Pichon, B., Leyssene, D., Bordes-Couecou, S., Meugnier, H., Rouard, C., Lemaitre, N., Schramm, F., Kearns, A., Spiliopoulou, I., Hyyryläinen, H.-L., Dumitrescu, O., Vandenesch, F., Dupieux, C., Laurent, F.

المصدر: Journal of Antimicrobial Chemotherapy ; page dkw516 ; ISSN 0305-7453 1460-2091

مصطلحات موضوعية: Infectious Diseases, Pharmacology (medical), Pharmacology, Microbiology (medical)

الإتاحة: https://doi.org/10.1093/jac/dkw516Test
http://academic.oup.com/jac/article-pdf/72/4/1014/11021545/dkw516.pdfTest

المؤلفون: Butin, Marine, Martins-Simões, Patricia, Pichon, B., Leyssene, D., Bordes-Couecou, S., Meugnier, Hélène, Rouard, C., Lemaitre, N., Schramm, F., Kearns, A., Spiliopoulou, I., Hyyryläinen, H.-L., Dumitrescu, Oana, Vandenesch, François, Dupieux, Céline, Laurent, François

المساهمون: Pathogénie des Staphylocoques – Staphylococcal Pathogenesis (StaPath), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Bactériologie de l'Hôpital de la Croix-Rousse, Hospices Civils de Lyon (HCL), Centre National de Reference des Staphylocoques, Université de Lyon, Public Health England London, Centre Hospitalier de la Côte Basque (CHCB), Hôpital Antoine Béclère, Université Paris-Sud - Paris 11 (UP11)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Virulence bactérienne précoce : fonctions cellulaires et contrôle de l'infection aiguë et subaiguë, Université de Strasbourg (UNISTRA), University of Patras, National Institute for Health and Welfare Helsinki

المصدر: ISSN: 0305-7453.

مصطلحات موضوعية: Adult, Aged, Anti-Bacterial Agents, Cluster Analysis, Drug Resistance, Bacterial, Electrophoresis, Gel, Pulsed-Field, Finland, France, Genes, Genome, Genotype, Greece, Humans, Linezolid, Male, Microbial Sensitivity Tests, Middle Aged, Molecular Epidemiology, Molecular Typing, Mutation, RNA, Ribosomal, 23S, Sequence Analysis, DNA, Staphylococcal Infections, Staphylococcus

الوصف: International audience ; Objectives: We investigated the epidemiological, clinical, microbiological and genetic characteristics of linezolid-resistant (LZR) Staphylococcus capitis isolates from French ICUs, and compared them with LZR S. capitis isolates from other European countries. Methods: All LZR isolates were subjected to antimicrobial susceptibility testing (AST) and the presence of cfr and optrA genes as well as mutations in the 23S rRNA and ribosomal proteins were investigated using specific PCR with sequencing. The genetic relationship between isolates was investigated using PFGE and WGS. Epidemiological data concerning LZR S. capitis were collected retrospectively in French microbiology laboratories. Results: Twenty-one LZR isolates were studied: 9 from France, 11 from Greece and 1 from Finland. All were resistant to methicillin and aminoglycosides. In addition, this unusual AST profile was identified in S. capitis isolates from seven French hospitals, and represented up to 12% of the S. capitis isolates in one centre. A G2576T mutation in 23S rRNA was identified in all isolates; cfr and optrA genes were absent. All isolates belonged to the same clone on the basis of their PFGE profiles, whatever their geographical origin. WGS found at most 212 SNPs between core genomes of the LZR isolates. Conclusions: We identified and characterized an LZR S. capitis clone disseminated in three European countries, harbouring the same multiple resistance and a G2576T mutation in the 23S rRNA. The possible unrecognized wider distribution of this clone, belonging to a species classically regarded as a low-virulence skin colonizer, is of major concern not least because of the increasing use of oxazolidinones.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/27999045; hal-01911046; https://hal.science/hal-01911046Test; PUBMED: 27999045

الإتاحة: https://doi.org/10.1093/jac/dkw516Test
https://hal.science/hal-01911046Test

المؤلفون: Cabasson, S., Godron, A., Bordes-Couecou, S., Hernandorena, X., Jouvencel, P.

المصدر: Archives de Pédiatrie ; volume 14, issue 3, page 294-295 ; ISSN 0929-693X

مصطلحات موضوعية: Pediatrics, Perinatology and Child Health

الإتاحة: https://doi.org/10.1016/j.arcped.2006.12.013Test
https://api.elsevier.com/content/article/PII:S0929693X06006348?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0929693X06006348?httpAccept=text/plainTest

المؤلفون: Butin, M., Martins-Simões, P., Pichon, B., Leyssene, D., Bordes-Couecou, S., Meugnier, H., Rouard, C., Lemaitre, N., Schramm, F., Kearns, A., Spiliopoulou, I., Hyyryläinen, H.-L., Dumitrescu, O., Vandenesch, F., Dupieux, C., Laurent, F.

المصدر: Journal of Antimicrobial Chemotherapy (JAC); Apr2017, Vol. 72 Issue 4, p1014-1020, 7p

مصطلحات موضوعية: LINEZOLID, STAPHYLOCOCCUS, ANTI-infective agents, RIBOSOMAL RNA, RIBOSOMAL proteins, ANTIBIOTICS, CLUSTER analysis (Statistics), COMPARATIVE studies, DRUG resistance in microorganisms, GENES, GENETIC techniques, GENOMES, RESEARCH methodology, MEDICAL cooperation, MICROBIAL sensitivity tests, MOLECULAR epidemiology, GENETIC mutation, PULSED-field gel electrophoresis, RESEARCH, RNA, STAPHYLOCOCCAL diseases, EVALUATION research, SEQUENCE analysis, GENOTYPES, PHARMACODYNAMICS

مصطلحات جغرافية: FINLAND, FRANCE, GREECE

مستخلص: Objectives: We investigated the epidemiological, clinical, microbiological and genetic characteristics of linezolid-resistant (LZR) Staphylococcus capitis isolates from French ICUs, and compared them with LZR S. capitis isolates from other European countries.Methods: All LZR isolates were subjected to antimicrobial susceptibility testing (AST) and the presence of cfr and optrA genes as well as mutations in the 23S rRNA and ribosomal proteins were investigated using specific PCR with sequencing. The genetic relationship between isolates was investigated using PFGE and WGS. Epidemiological data concerning LZR S. capitis were collected retrospectively in French microbiology laboratories.Results: Twenty-one LZR isolates were studied: 9 from France, 11 from Greece and 1 from Finland. All were resistant to methicillin and aminoglycosides. In addition, this unusual AST profile was identified in S. capitis isolates from seven French hospitals, and represented up to 12% of the S. capitis isolates in one centre. A G2576T mutation in 23S rRNA was identified in all isolates; cfr and optrA genes were absent. All isolates belonged to the same clone on the basis of their PFGE profiles, whatever their geographical origin. WGS found at most 212 SNPs between core genomes of the LZR isolates.Conclusions: We identified and characterized an LZR S. capitis clone disseminated in three European countries, harbouring the same multiple resistance and a G2576T mutation in the 23S rRNA. The possible unrecognized wider distribution of this clone, belonging to a species classically regarded as a low-virulence skin colonizer, is of major concern not least because of the increasing use of oxazolidinones. [ABSTRACT FROM AUTHOR]

: Copyright of Journal of Antimicrobial Chemotherapy (JAC) is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Bordes^Couecou, S., Gaillot, O., Simonet, M.

المصدر: Médecine et Maladies Infectieuses ; volume 26, issue 6-7, page 721-725 ; ISSN 0399-077X

مصطلحات موضوعية: Infectious Diseases

الإتاحة: https://doi.org/10.1016/s0399-077xTest(96)80103-x
https://api.elsevier.com/content/article/PII:S0399077X9680103X?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0399077X9680103X?httpAccept=text/plainTest