المؤلفون: Björn J. Sieberer, Antonius C. J. Timmers, Anne Mie C. Emons

المصدر: Molecular Plant-Microbe Interactions, Vol 18, Iss 11, Pp 1195-1204 (2005)

مصطلحات موضوعية: Microbiology, QR1-502, Botany, QK1-989

الوصف: The microtubule (MT) cytoskeleton is an important part of the tip-growth machinery in legume root hairs. Here we report the effect of Nod factor (NF) on MTs in root hairs of Medicago truncatula. In tip-growing hairs, the ones that typically curl around rhizobia, NF caused a subtle shortening of the endoplasmic MT array, which recovered within 10 min, whereas cortical MTs were not visibly affected. In growth-arresting root hairs, endoplasmic MTs disappeared shortly after NF application, but reformed within 20 min, whereas cortical MTs remained present in a high density. After NF treatment, growth-arresting hairs were swelling at their tips, after which a new outgrowth formed that deviated with a certain angle from the former growth axis. MT depolymerization with oryzalin caused a growth deviation similar to the NF; whereas, combined with NF, oryzalin increased and the MT-stabilizing drug taxol suppressed NF-induced growth deviation. The NF-induced disappearance of the endoplasmic MTs correlated with a loss of polar cytoarchitecture and straight growth directionality, whereas the reappearance of endoplasmic MTs correlated with the new set up of polar cytoarchitecture. Drug studies showed that MTs are involved in determining root hair elongation in a new direction after NF treatment.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/0894-0282Test; https://doaj.org/toc/1943-7706Test

الوصول الحر: https://doaj.org/article/f5cc52d711c14cd489c9da7357814142Test

المؤلفون: David G. Barker, Björn J. Sieberer, Antonius C.J. Timmers, Mireille Chabaud, Joëlle Fournier

المصدر: The Plant Journal. 69:822-830

مصطلحات موضوعية: 0106 biological sciences, 0303 health sciences, biology, fungi, Cell Biology, Plant Science, Cameleon (protein), biology.organism_classification, 01 natural sciences, Apoplast, Medicago truncatula, Cell biology, 03 medical and health sciences, Botany, Genetics, biology.protein, Transcellular, Signal transduction, Reprogramming, Intracellular, 030304 developmental biology, 010606 plant biology & botany, Calcium signaling

الوصف: Ca(2+) spiking is a central component of a common signaling pathway that is activated in the host epidermis during initial recognition of endosymbiotic microbes. However, it is not known to what extent Ca(2+) signaling also plays a role during subsequent root colonization involving apoplastic transcellular infection. Live-tissue imaging using calcium cameleon reporters expressed in Medicago truncatula roots has revealed that distinct Ca(2+) oscillatory profiles correlate with specific stages of transcellular cortical infection by both rhizobia and arbuscular mycorrhizal fungi. Outer cortical cells exhibit low-frequency Ca(2+) spiking during the extensive intracellular remodeling that precedes infection. This appears to be a prerequisite for the formation of either pre-infection threads or the pre-penetration apparatus, both of which are fully reversible processes. A transition from low- to high-frequency spiking is concomitant with the initial stages of apoplastic cell entry by both microbes. This high-frequency spiking is of limited duration in the case of rhizobial infection and is completely switched off by the time transcellular infection by both microsymbionts is completed. The Ca(2+) spiking profiles associated with both rhizobial and arbuscular mycorrhizal cell entry are remarkably similar in terms of periodicity, suggesting that microbe specificity is unlikely to be encoded by the Ca(2+) signature during this particular stage of host infection in the outer cortex. Together, these findings lead to the proposal that tightly regulated Ca(2+) -mediated signal transduction is a key player in reprogramming root cell development at the critical stage of commitment to endosymbiotic infection.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::dffd755100deb7aab91aa09e3ab52692Test
https://doi.org/10.1111Test/j.1365-313x.2011.04834.x

المؤلفون: Tiny Franssen-Verheijen, Anne Mie C. Emons, Björn J. Sieberer, Henk Kieft, Jan W. Vos

المصدر: Planta
Planta 230 (2009) 6
Planta, 230(6), 1129-1140

مصطلحات موضوعية: 0106 biological sciences, soyuz-missions, cortical microtubules, Plant Science, 01 natural sciences, Microtubules, Cortical microtubule cytoskeleton, TUBUL experiment, random-positioning machine, Cytoskeleton, Tobacco BY-2 suspension culture cells, 0303 health sciences, Microscopy, Confocal, EPS-1, food and beverages, plant-cells, Immunohistochemistry, Cell biology, Cellulose microfibril ordering, Original Article, Cortical microtubule, actin, Tobacco BY-2 cells, growth, Biology, Cell Line, 03 medical and health sciences, Microtubule, Tobacco, Genetics, Plant cell growth, Cellulose, Microtubule ordering, Cell Shape, 030304 developmental biology, Microtubule nucleation, Cell Proliferation, Weightlessness, Cellulose microfibril organization, fungi, Laboratorium voor Celbiologie, Space Flight, microgravity, self-organization, gravity, Plant cell division, Laboratory of Cell Biology, arabidopsis, Tubulin, biology.protein, Microscopy, Electron, Scanning, Microgravity, 010606 plant biology & botany

الوصف: The microtubule cytoskeleton and the cell wall both play key roles in plant cell growth and division, determining the plant's final stature. At near weightlessness, tubulin polymerizes into microtubules in vitro, but these microtubules do not self-organize in the ordered patterns observed at 1g. Likewise, at near weightlessness cortical microtubules in protoplasts have difficulty organizing into parallel arrays, which are required for proper plant cell elongation. However, intact plants do grow in space and therefore should have a normally functioning microtubule cytoskeleton. Since the main difference between protoplasts and plant cells in a tissue is the presence of a cell wall, we studied single, but walled, tobacco BY-2 suspension-cultured cells during an 8-day space-flight experiment on board of the Soyuz capsule and the International Space Station during the 12S mission (March-April 2006). We show that the cortical microtubule density, ordering and orientation in isolated walled plant cells are unaffected by near weightlessness, as are the orientation of the cellulose microfibrils, cell proliferation, and cell shape. Likely, tissue organization is not essential for the organization of these structures in space. When combined with the fact that many recovering protoplasts have an aberrant cortical microtubule cytoskeleton, the results suggest a role for the cell wall, or its production machinery, in structuring the microtubule cytoskeleton.

وصف الملف: application/pdf; application/octet-stream

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f8ed979925d23d33dd271c8d7e54416aTest
http://europepmc.org/articles/PMC2764053Test

المؤلفون: Jan W. Vos, Björn J. Sieberer, Anne Mie C. Emons

المصدر: Microgravity-Science and Technology, 19(5-6), 191-195
Microgravity-Science and Technology 19 (2007) 5-6

مصطلحات موضوعية: Chromatography, EPS-1, Cell division, Chemistry, Applied Mathematics, Microtubule cytoskeleton, General Engineering, General Physics and Astronomy, Nylon mesh, Laboratorium voor Celbiologie, Nanotechnology, Liquid medium, Plant cell, microtubules, On board, Laboratory of Cell Biology, chemistry.chemical_compound, Modeling and Simulation, Agarose, Stationary growth

الوصف: For the TUBUL experiments during the DELTA mission in April 2004 and 12S mission in March/April 2006 on board the Soyuz capsule and the International Space Station we developed a method to culture and chemically fix plant suspension culture cells. The aim of the ten day experiment was to investigate the effect of microgravity on single plant cells. Fully automated experiment cassettes (Plunger Box Units) were developed by Centre for Concepts in Mechatronics (Nuenen, the Netherlands). Tobacco BY- 2 cells were immobilized in a semi- solid agarose matrix that was reinforced by a nylon mesh. This assembly allowed liquid medium refreshment, oxygen supply and chemical fixation, including a post- fixative wash. The method was optimized for post- flight analysis of cell structure,shape and size, cell division, and the microtubule cytoskeleton. The viability of cells in the agarose matrix was similar to cells grown in liquid medium under laboratory conditions, only the stationary growth phase was reached six days later

وصف الملف: application/octet-stream; application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c928d016ba92415b34ffe3f6bc215958Test
https://doi.org/10.1007/bf02919480Test

المؤلفون: Mireille Chabaud, David G. Barker, Björn J. Sieberer, Antonius C.J. Timmers, Joëlle Fournier

المساهمون: Laboratoire des interactions plantes micro-organismes (LIPM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), ANR project ANR-08-BLAN-0029-01, LABEX TULIP (ANR-10-LABX-41)

المصدر: Biological Nitrogen Fixation
Biological Nitrogen Fixation, de Bruijn, Frans J., Wiley-Blackwell, 2015, ⟨10.1002/9781119053095.ch57⟩

مصطلحات موضوعية: 0106 biological sciences, 0303 health sciences, biology, [SDV]Life Sciences [q-bio], Bacterial host, Root hair, biology.organism_classification, 01 natural sciences, Medicago truncatula, 03 medical and health sciences, Botany, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS, Ca2 signaling, 030304 developmental biology, 010606 plant biology & botany

الوصف: International audience

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c30f14310c61913f60aaa0b486144a4aTest
https://doi.org/10.1002/9781119053095.ch57Test

المؤلفون: Antonius C.J. Timmers, Björn J. Sieberer, Anne Mie C. Emons

المصدر: Molecular Plant-Microbe Interactions 18 (2005) 11
Molecular Plant-Microbe Interactions, 18(11), 1195-1204

مصطلحات موضوعية: Lipopolysaccharides, preprophase band formation, Paclitaxel, Physiology, rhizobium-meliloti, Root hair elongation, Root hair, Biology, Microtubules, Plant Roots, Nod factor, Microtubule, Medicago truncatula, Sulfanilamides, Tip growth, Symbiosis, Cytoskeleton, EPS-1, receptor kinase gene, Endoplasmic reticulum, endoplasmic microtubules, tip growth, Laboratorium voor Celbiologie, General Medicine, biology.organism_classification, Tubulin Modulators, nodulation factors, Cell biology, Dinitrobenzenes, Laboratory of Cell Biology, vicia-sativa, Biochemistry, lipo-oligosaccharide signals, f-actin, Agronomy and Crop Science, Rhizobium, Signal Transduction, symbiotic host-specificity

الوصف: The microtubule (MT) cytoskeleton is an important part of the tip-growth machinery in legume root hairs. Here we report the effect of Nod factor (NF) on MTs in root hairs of Medicago truncatula. In tip-growing hairs, the ones that typically curl around rhizobia, NF caused a subtle shortening of the endoplasmic MT array, which recovered within 10 min, whereas cortical MTs were not visibly affected. In growth-arresting root hairs, endoplasmic MTs disappeared shortly after NF application, but reformed within 20 min, whereas cortical MTs remained present in a high density. After NF treatment, growth-arresting hairs were swelling at their tips, after which a new outgrowth formed that deviated with a certain angle from the former growth axis. MT depolymerization with oryzalin caused a growth deviation similar to the NF; whereas, combined with NF, oryzalin increased and the MT-stabilizing drug taxol suppressed NF-induced growth deviation. The NF-induced disappearance of the endoplasmic MTs correlated with a loss of polar cytoarchitecture and straight growth directionality, whereas the reappearance of endoplasmic MTs correlated with the new set up of polar cytoarchitecture. Drug studies showed that MTs are involved in determining root hair elongation in a new direction after NF treatment.

وصف الملف: application/pdf; application/octet-stream

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba9bb7cd8a5a8a7897c428f5db1f27c6Test
https://doi.org/10.1094/mpmi-18-1195Test

المؤلفون: J.J. Esseling, Björn J. Sieberer, Anne Mie C. Emons, Tijs Ketelaar

المصدر: New Phytologist 167 (2005) 3
New Phytologist, 167(3), 711-719

مصطلحات موضوعية: preprophase band formation, Cell division, cortical microtubules, Physiology, phospholipase-d, Plant Science, arabidopsis-thaliana, Root hair, Biology, Microtubules, Plant Roots, rhizobium nod factors, Schizosaccharomyces, Cell cortex, Cell polarity, Tip growth, free calcium, EPS-1, Cell growth, Cell Polarity, endoplasmic microtubules, plasma-membrane, Laboratorium voor Celbiologie, Plants, Actin cytoskeleton, fission yeast, Cell biology, Laboratory of Cell Biology, Cytoplasm, medicago-truncatula

الوصف: The ability to establish cell polarity is crucial to form and function of an individual cell. Polarity underlies critical processes during cell development, such as cell growth, cell division, cell differentiation and cell signalling. Interphase cytoplasmic microtubules in tip-growing fission yeast cells have been shown to play a particularly important role in regulating cell polarity. By placing proteins that serve as spatial cues in the cell cortex of the expanding tip, microtubules determine the site where exocytosis, and therefore growth, takes place. Transport and the targeting of exocytotic vesicles to the very tip depend on the actin cytoskeleton. Recently, endoplasmic microtubules have been identified in tip-growing root hairs, which are an experimental system for plant cell growth. Here, we review the data that demonstrate involvement of microtubules in hair elongation and polarity of the model plants Medicago truncatula and Arabidopsis thaliana. Differences and similarities between the microtubule organization and function in these two species are discussed and we compare the observations in root hairs with the microtubule-based polarity mechanism in fission yeast.

وصف الملف: application/pdf; application/octet-stream

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95d86da07b86c3c29cda19f5c0815655Test
https://doi.org/10.1111Test/j.1469-8137.2005.01506.x

المؤلفون: Franck Lhuissier, Antonius C.J. Timmers, Björn J. Sieberer, Anne Mie C. Emons

المصدر: Plant Physiology, 130, 977-988
Plant Physiology 130 (2002)

مصطلحات موضوعية: Laboratorium voor Plantencelbiologie, Cytoplasm, Paclitaxel, Physiology, Green Fluorescent Proteins, Population, Plant Science, Biology, Root hair, Endoplasmic Reticulum, Microtubules, Plant Roots, chemistry.chemical_compound, Microtubule, Sulfanilamides, Botany, Medicago, Genetics, medicine, Life Science, Cytoskeleton, education, education.field_of_study, integumentary system, Cell Polarity, Oryzalin, Immunohistochemistry, Cell biology, Laboratory of Plant Cell Biology, Dinitrobenzenes, Luminescent Proteins, medicine.anatomical_structure, chemistry, Ultrastructure, Cell Surface Extensions, EPS, Carrier Proteins, Nucleus, Cell Division, Research Article

الوصف: To investigate the configuration and function of microtubules (MTs) in tip-growing Medicago truncatularoot hairs, we used immunocytochemistry or in vivo decoration by a GFP linked to a MT-binding domain. The two approaches gave similar results and allowed the study of MTs during hair development. Cortical MTs (CMTs) are present in all developmental stages. During the transition from bulge to a tip-growing root hair, endoplasmic MTs (EMTs) appear at the tip of the young hair and remain there until growth arrest. EMTs are a specific feature of tip-growing hairs, forming a three-dimensional array throughout the subapical cytoplasmic dense region. During growth arrest, EMTs, together with the subapical cytoplasmic dense region, progressively disappear, whereas CMTs extend further toward the tip. In full-grown root hairs, CMTs, the only remaining population of MTs, converge at the tip and their density decreases over time. Upon treatment of growing hairs with 1 μm oryzalin, EMTs disappear, but CMTs remain present. The subapical cytoplasmic dense region becomes very short, the distance nucleus tip increases, growth slows down, and the nucleus still follows the advancing tip, though at a much larger distance. Taxol has no effect on the cytoarchitecture of growing hairs; the subapical cytoplasmic dense region remains intact, the nucleus keeps its distance from the tip, but growth rate drops to the same extent as in hairs treated with 1 μm oryzalin. The role of EMTs in growing root hairs is discussed.

وصف الملف: application/octet-stream; application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2947eb1becd95293b399f959141856bdTest
https://doi.org/10.1104/pp.004267Test

المؤلفون: N.C.A. de Ruijter, Björn J. Sieberer, Franck Lhuissier, J.J. Esseling, Anne Mie C. Emons

المصدر: Annals of Botany, 87, 289-302
Annals of Botany 87 (2001)

مصطلحات موضوعية: Laboratorium voor Plantencelbiologie, Plant Science, Nod, Review, Biology, Root hair, Nod factor, Botany, Tip growth, Cytoskeleton, Symbiosis, integumentary system, Depolarization, Actin cytoskeleton, biology.organism_classification, Cell biology, Legume, Laboratory of Plant Cell Biology, Rhizobium, Calcium, sense organs, EPS

الوصف: In many common legumes, when host-specific nodule bacteria meet their legume root they attach to it and enter through root hairs. The bacteria can intrude these cells because they instigate in the hairs the formation of an inward growing tube, the infection thread, which consists of wall material. Prior to infection thread formation, the bacteria exploit the cell machinery for wall deposition by inducing the hairs to form a curl, in which the dividing bacteria become entrapped. In most species, Nod factor alone (a lipochito-oligosaccharide excreted by bacteria) induces root hair deformation, though without curling, thus most aspects of the initial effects of Nod factor can be elucidated by studying root hair deformation. In this review we discuss the cellular events that host-specific Nod factors induce in their host legume root hairs. The first event, detectable only a few seconds after Nod factor application, is a Ca 2+ influx at the root hair tip, followed by a transient depolarization of the plasma membrane potential, causing an increase in cytosolic [Ca 2+ ] at the root hair tip. Also within minutes, Nod factors change the cell organization by acting on the actin cytoskeleton, enhancing tip cell wall deposition so that root hairs become longer than normal for their species. Since the remodelling of the actin cytoskeleton precedes the second calcium event, Ca 2+ spiking, which is observed in the perinuclear area, we propose that the initial cytoskeleton events taking place at the hair tip are related to Ca 2+ influx in the hair tip and that Ca 2+ spiking serves later events involving gene expression.

وصف الملف: application/octet-stream; application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30c51bb27791996ff3c8d8a95d8abd16Test
https://doi.org/10.1006/anbo.2000.1333Test

المؤلفون: Björn J. Sieberer, Anne Mie C. Emons

المصدر: Protoplasma, 214, 118-127
Protoplasma 214 (2000)

مصطلحات موضوعية: Laboratorium voor Plantencelbiologie, Lipochito-oligosaccharides, Video microscopy, Plant Science, Root hair, Organelle, Botany, Medicago truncatula, Tip growth, Organelle movement, integumentary system, biology, Cell Biology, General Medicine, biology.organism_classification, Plant-rhizobium interaction, Cell biology, Cytoplasmic streaming, Laboratory of Plant Cell Biology, Cytoarchitecture, Cytoplasm, sense organs, EPS

الوصف: The cytoarchitecture and the pattern of cytoplasmic streaming change during the development of root hairs ofMedicago truncatula and after a challenge with nodulation (Nod) factors. We measured the speed and orientation of movement of 1–2 μm long organelles. The speed of organelle movement in cytoplasmic strands in the basal part of growing root hairs is 8–14 μm/s and is of the circulation type like in trichoblasts, bulges before tip-growth initiation, and full-grown hairs. In the subapical area of growing hairs, reverse-fountain streaming occurs discontinuously at a slower net speed. The reason for the slower speed is the fact that organelles often stop and jump. Reverse-fountain streaming is a pattern in which the main direction of organelle transport reverses 180 degrees before the cell tip is reached. Within minutes after their application to roots,Rhizobium leguminosarum-derived Nod factors, cause an increase and divergence in the subapical cytoplasmic strands. This phenomenon can best be observed in the growth-terminating hairs, since in hairs of this developmental stage, subapical cytoplasmic strands are transvacuolar. First, the tips of these hairs swell. The organelle movement in the swelling tip increases up to the level normal for circulation streaming, and the number of strands with moving organelles increases. When a new polar outgrowth emerges, reverse-fountain streaming is set up again, with all its characteristics like those seen in growing hairs. This outgrowth may obtain a new full root hair length, by which these hairs may become twice as long as nonchallenged hairs.

وصف الملف: application/octet-stream; text/html

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d5369e82a75d35a01bdd2cbe2c19c341Test
https://doi.org/10.1007/bf02524268Test