المؤلفون: Mengyang Sun, Hao Liu, Bingqing Zhu, Yang Liu, Aijia Li, Limei Wang

المصدر: Frontiers in Neurology, Vol 14 (2023)

مصطلحات موضوعية: glial fibrillary acidic protein (GFAP), myelin oligodendrocyte glycoprotein (MOG), myelitis, cerebrospinal fluid, overlapping antibodies, Neurology. Diseases of the nervous system, RC346-429

الوصف: ObjectiveGlial fibrillary acidic protein-immunoglobulin G (GFAP-IgG)-associated myelitis and myelin oligodendrocyte glycoprotein-IgG (MOG-IgG)-associated myelitis have rarely been compared. Therefore, this study aimed to explore the clinical, laboratory, and imaging features of them to identify the differences.MethodsOverall, 14 and 24 patients with GFAP-IgG-and MOG-IgG-associated myelitis, respectively, were retrospectively screened and included in the study.ResultsAmong the 14 patients with GFAP-IgG-associated myelitis, the condition was more common in males (71.4%), with a median age of onset of 36.5 years, and more common in adults than in children (35.7%). In contrast, among the 24 patients with MOG-IgG-associated myelitis, the condition was equally divided between males and females, with a median age of onset of 9.5 years and more in children (66.7%) than in adults. The median age of onset of GFAP-IgG-associated myelitis was later than that of the MOG-IgG group. Isolated myelitis was rare in both groups. Elevated cerebrospinal fluid (CSF) protein levels were more prevalent in patients with GFAP-IgG-associated myelitis (64.3%) than in those with MOG-IgG-associated myelitis (16.7%) (p

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fneur.2023.1266067/fullTest; https://doaj.org/toc/1664-2295Test

الوصول الحر: https://doaj.org/article/905b2a6a92664ec284d207d7a060ba01Test

المؤلفون: Bingqing Zhu, Mengyang Sun, Ting Yang, Haizhen Yu, Limei Wang

المصدر: Frontiers in Immunology, Vol 14 (2023)

مصطلحات موضوعية: glial fibrillary astrocytic protein antibodies, clinical characteristics, imaging features, overlapping antibodies, prognosis, Immunologic diseases. Allergy, RC581-607

الوصف: ObjectiveTo evaluate and compare the clinical features, imaging, overlapping antibodies, and prognosis of pediatric and adult patients with anti-GFAP antibodies.MethodsThis study included 59 patients with anti-GFAP antibodies (28 females and 31 males) who were admitted between December 2019 and September 2022.ResultsOut of 59 patients, 18 were children (under 18 years old), and 31 were adults. The overall cohort’s median age at onset was 32 years old, 7 for children, and 42 for adults. There were 23 (41.1%) patients with prodromic infection, 1 (1.7%) patient with a tumor, 29 (53.7%) patients with other non-neurological autoimmune diseases, and 17 (22.8%) patients with hyponatremia. Fourteen (23.7%) patients had multiple neural autoantibodies, with the AQP4 antibody being the most common. Encephalitis (30.5%) was the most common phenotypic syndrome. Common clinical symptoms included fever (59.3%), headache (47.5%), nausea and vomiting (35.6%), limb weakness (35.6%), and disturbance of consciousness (33.9%). Brain MRI lesions were primarily located in the cortex/subcortex (37.3%), brainstem (27.1%), thalamus (23.7%), and basal ganglia (22.0%). Spinal cord MRI lesions often involved the cervical and thoracic spinal cord. There was no statistically significant difference in the MRI lesion site between children and adults. Out of 58 patients, 47 (81.0%) had a monophasic course, and 4 died. The last follow-up showed that 41/58 (80.7%) patients had an improved functional outcome (mRS

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fimmu.2023.1106490/fullTest; https://doaj.org/toc/1664-3224Test

الوصول الحر: https://doaj.org/article/c8a97f48f31b4af680e63555102a7036Test

المؤلفون: Pan Zhao, Li Xu, Aiyu Zhang, Bingqing Zhu, Zhujun Shao

المصدر: BMC Microbiology, Vol 20, Iss 1, Pp 1-12 (2020)

مصطلحات موضوعية: DNA gyrase subunit A, Quinolone Resistance-Determining Region, Neisseria meningitidis clonal complex 4821, Recombination, Microbiology, QR1-502

الوصف: Abstract Background Neisseria meningitidis (N.meningitidis) bacteria belonging to clonal complex 4821 (CC4821) have been mainly reported in China and have been characterized by a high resistance rate to ciprofloxacin (CIP). The aim of this study was to assess the evolution of the DNA gyrase A (gyrA) gene from N.meningitidis CC4821 strains collected in China between 1978 and 2016. The complete sequence of gyrA gene from 77 strains are reported in this study and analyzed in the context of publicly available sequences from N. meningitidis of other CCs as well as other Neisseria species. Results The phylogenetic analysis of CC4821 gyrA gene reveals at least 5 distinct genetic clusters. These clusters are not CC4821-specific showing that gyrA evolution is independent of CC4821 evolution. Some clusters contain sequences from other Neisseria species. Recombination within N.meningitidis strains and between Neisseria species was identified in SimPlot analysis. Finally, amino acid substitutions within GyrA protein were analyzed. Only one position, 91 (83 in E.coli gyrA gene), was linked to CIP resistance. Thirty-one additional putative resistance markers were identified, as amino acid substitutions were only found in resistant strains. Conclusions The evolution of gyrA gene of CC4821 N.meningitidis strains is not dependent on CC4821 evolution or on CIP resistance phenotype. Only amino acid 91 is linked to CIP resistance phenotype. Finally, recombination inter- and intra-species is likely to result in the acquisition of various resistance markers, 31 of them being putatively mapped in the present study. Analyzing the evolution of gyrA gene within CC4821 strains is critical to monitor the CIP resistance phenotype and the acquisition of new resistance markers. Such studies are necessary for the control of the meningococcal disease and the development of new drugs targeting DNA gyrase.

وصف الملف: electronic resource

العلاقة: http://link.springer.com/article/10.1186/s12866-020-01751-5Test; https://doaj.org/toc/1471-2180Test

الوصول الحر: https://doaj.org/article/2692b8754e6c4960a03d3004e71bb4b4Test

المؤلفون: Reinaldo Acevedo, Xilian Bai, Ray Borrow, Dominique A. Caugant, Josefina Carlos, Mehmet Ceyhan, Hannah Christensen, Yanet Climent, Philippe De Wals, Ener Cagri Dinleyici, Gabriela Echaniz-Aviles, Ahmed Hakawi, Hajime Kamiya, Andromachi Karachaliou, Jay Lucidarme, Susan Meiring, Konstantin Mironov, Marco A. P. Sáfadi, Zhujun Shao, Vinny Smith, Robert Steffen, Bianca Stenmark, Muhamed-Kheir Taha, Caroline Trotter, Julio A. Vázquez, Bingqing Zhu

المصدر: Expert Review of Vaccines, Vol 18, Iss 1, Pp 15-30 (2019)

مصطلحات موضوعية: antibiotic resistance, bacterial meningitis, conjugate vaccine, epidemiology, immunization program, meningococcal disease, neisseria meningitidis, polysaccharide vaccine, serogroup, surveillance, vaccine, Internal medicine, RC31-1245

الوصف: Introduction: The 2018 Global Meningococcal Initiative (GMI) meeting focused on evolving invasive meningococcal disease (IMD) epidemiology, surveillance, and protection strategies worldwide, with emphasis on emerging antibiotic resistance and protection of high-risk populations. The GMI is comprised of a multidisciplinary group of scientists and clinicians representing institutions from several continents. Areas covered: Given that the incidence and prevalence of IMD continually varies both geographically and temporally, and surveillance systems differ worldwide, the true burden of IMD remains unknown. Genomic alterations may increase the epidemic potential of meningococcal strains. Vaccination and (to a lesser extent) antimicrobial prophylaxis are the mainstays of IMD prevention. Experiences from across the globe advocate the use of conjugate vaccines, with promising evidence growing for protein vaccines. Multivalent vaccines can broaden protection against IMD. Application of protection strategies to high-risk groups, including individuals with asplenia, complement deficiencies and human immunodeficiency virus, laboratory workers, persons receiving eculizumab, and men who have sex with men, as well as attendees at mass gatherings, may prevent outbreaks. There was, however, evidence that reduced susceptibility to antibiotics was increasing worldwide. Expert commentary: The current GMI global recommendations were reinforced, with several other global initiatives underway to support IMD protection and prevention.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1476-0584Test; https://doaj.org/toc/1744-8395Test

الوصول الحر: https://doaj.org/article/d704b0220a7945b682920a34130ef5bcTest

المؤلفون: Jay Lucidarme, Bingqing Zhu, Li Xu, Xilian Bai, Yuan Gao, Juan José González-López, Robert Mulhall, Kevin J Scott, Andrew Smith, Paola Stefanelli, Bianca Stenmark, Paul Torpiano, Georgina Tzanakaki, Ray Borrow, Zhujun Shao

المصدر: PLoS ONE, Vol 15, Iss 12, p e0243426 (2020)

مصطلحات موضوعية: Medicine, Science

الوصف: IntroductionThe ST-4821 complex (cc4821) is a leading cause of serogroup C and serogroup B invasive meningococcal disease in China where diverse strains in two phylogenetic groups (groups 1 and 2) have acquired fluoroquinolone resistance. cc4821 was recently prevalent among carriage isolates in men who have sex with men in New York City (USA). Genome-level population studies have thus far been limited to Chinese isolates. The aim of the present study was to build upon these with an extended panel of international cc4821 isolates.MethodsGenomes of isolates from Asia (1972 to 2017), Europe (2011 to 2018), North America (2007), and South America (2014) were sequenced or obtained from the PubMLST Neisseria database. Core genome comparisons were performed in PubMLST.ResultsFour lineages were identified. Western isolates formed a distinct, mainly serogroup B sublineage with alleles associated with fluoroquinolone susceptibility (MIC DiscussionThe previously identified cc4821 group 2 was resolved into three separate lineages. Clustering of western isolates was surprising given the overall diversity of cc4821. Possible association of this cluster with the anogenital niche is worthy of monitoring given concerns surrounding antibiotic resistance and potential subcapsular vaccine escape.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1932-6203Test

الوصول الحر: https://doaj.org/article/7fdc16acd1d74624bc34f6c4b7b35293Test

المؤلفون: Aiyu Zhang, Pan Zhao, Bingqing Zhu, Fenglin Shi, Li Xu, Yuan Gao, Na Xie, Zhujun Shao

المصدر: Frontiers in Cellular and Infection Microbiology, Vol 7 (2017)

مصطلحات موضوعية: autB, Neisseria meningitidis, autotransporter, characteristic, distribution, DNA uptake sequence, Microbiology, QR1-502

الوصف: We aimed to investigate and understand the characterization and distribution of the autB gene in Neisseria meningitidis in China. autB is flanked by two conservative genes, smpB and glcD, and it can be present in the majority of meningococcal isolates, but not in 053442 of clonal complex 4821 (CC4821) which contains a 968 bp sequence. In this study, we sequenced the intervenient region between smpB and glcD in 178 Chinese N. meningitidis strains isolated from both patients and carriers. There were 110 serogroupable strains, other 68 were non-groupable (NG). Ninety nine of the 178 strains were clustered into 13 CCs, the remaining 79 were unassigned (UA). CC4821 is one of the dominant CCs in China. Forty of the 42 CC4821 strains and 26 of the 79 UA strains were autB-null, while the remaining 12 CCs were autB-positive. According to the N-terminal sequence, most (97/112) of the autB-positive strains were clustered into AutB1 and the remaining 15 were AutB2. The autB gene and its flanking intergenic sequences was superseded by a perfectly conservative sequence of an identical 968 bp in all of the autB-null N. meningitidis strains which had no identity with the relatively conservative intergenic sequences that flanked the autB gene in autB-positive strains. There was a 10 bp DNA uptake sequence (DUS) at the beginning of the interval 968 bp sequence in the autB-null strains while there was a 9 bp Haemophilus-specific uptake sequence (hUS) at the beginning of the partial holB gene and at the end of the partial tmk gene in autB-positive strains, holB and tmk gene were flanking the autB gene in Haemophilus. In conclusion, not all pathogenic N. meningitidis strains especially CC4821 possess the autB gene in China and the corresponding spacer region of the autB-null strains was not homologous to that found in autB-positive strains. There's a hypothesis that the DUS and hUS are likely to play a key part in the mechanism of uptake or loss of the autB gene.

وصف الملف: electronic resource

العلاقة: http://journal.frontiersin.org/article/10.3389/fcimb.2017.00436/fullTest; https://doaj.org/toc/2235-2988Test

الوصول الحر: https://doaj.org/article/5002b0716819426faf1baae6bad195fcTest

المؤلفون: Bingqing Zhu, Zheng Xu, Pengcheng Du, Li Xu, Xiaofang Sun, Yuan Gao, Zhujun Shao

المصدر: Emerging Infectious Diseases, Vol 21, Iss 6, Pp 925-932 (2015)

مصطلحات موضوعية: Neisseria meningitidis, serogroup B, serogroup C, sequence type 4821, ST-4821 clonal complex, clonal complex 4821, Medicine, Infectious and parasitic diseases, RC109-216

الوصف: Serogroup B Neisseria meningitidis strains belonging to sequence type 4821 clonal complex (CC4821), a hyperinvasive lineage first identified for serogroup C in 2003, have been increasingly isolated in China. We characterized the outer membrane protein genes of 48 serogroup B and 214 serogroup C strains belonging to CC4821 and analyzed the genomic sequences of 22 strains. Four serogroup B strains had porin A (i.e., PorA), PorB, and ferric enterobactin transport (i.e., FetA) genotypes identical to those for serogroup C. Phylogenetic analysis of the genomic sequences showed that the 22 CC4821 strains from patients and healthy carriers were unevenly clustered into 2 closely related groups; each group contained serogroup B and C strains. Serogroup B strains appeared variable at the capsule locus, and several recombination events had occurred at uncertain breakpoints. These findings suggest that CC4821 serogroup C N. meningitidis is the probable origin of highly pathogenic CC4821 serogroup B strains.

وصف الملف: electronic resource

العلاقة: https://wwwnc.cdc.gov/eid/article/21/6/14-0687_articleTest; https://doaj.org/toc/1080-6040Test; https://doaj.org/toc/1080-6059Test

الوصول الحر: https://doaj.org/article/ba91610d07f94900b40ff1bc80a8bcf6Test

المؤلفون: Yixing Li, Zundong Yin, Zhujun Shao, Manshi Li, Xiaofeng Liang, Hardeep S. Sandhu, Stephen C. Hadler, Junhong Li, Yinqi Sun, Jing Li, Wenjing Zou, Mei Lin, Shuyan Zuo, Leonard W. Mayer, Ryan T. Novak, Bingqing Zhu, Li Xu, Huiming Luo

المصدر: Emerging Infectious Diseases, Vol 20, Iss 1, Pp 61-69 (2014)

مصطلحات موضوعية: bacterial meningitis, meningitis, pneumococcal, Neisseria meningitides, Haemophilus influenzae type b, bacteria, Medicine, Infectious and parasitic diseases, RC109-216

الوصف: During September 2006–December 2009, we conducted active population and sentinel laboratory–based surveillance for bacterial meningitis pathogens, including Streptococcus pneumoniae, Neisseria meningitidis, and Haemophilus influenzae type b, in 4 China prefectures. We identified 7,876 acute meningitis and encephalitis syndrome cases, including 6,388 among prefecture residents. A total of 833 resident cases from sentinel hospitals met the World Health Organization case definition for probable bacterial meningitis; 339 of these cases were among children

وصف الملف: electronic resource

العلاقة: https://wwwnc.cdc.gov/eid/article/20/1/12-0375_articleTest; https://doaj.org/toc/1080-6040Test; https://doaj.org/toc/1080-6059Test

الوصول الحر: https://doaj.org/article/02bba6872f724dbbbf99ddf029940c74Test

المؤلفون: Xiaoying Shan, Haijian Zhou, Ji Zhang, Bingqing Zhu, Li Xu, Guangchun Hu, Aiying Bai, Zhujun Shao, Baofa Jiang

المصدر: PLoS ONE, Vol 10, Iss 1, p e0116422 (2015)

مصطلحات موضوعية: Medicine, Science

الوصف: Neisseria meningitidis (N. meningitidis) serogroup C sequence type (ST)-4821 caused an outbreak in 2010 in Shandong province of China. Twenty-one non-outbreak-associated strains were isolated, along with twenty-eight N. meningitides serogroup C ST-4821 isolates. Therefore, it's essential to identify and clarify characterization of the real outbreak-associated strains with a rapid method during an outbreak investigation. In this study, multiple-locus variable number tandem repeat analysis (MLVA) was applied to analyze 84 N. meningitidis strains, among which 58 were recovered from two outbreaks and 26 were sporadic isolates. Three MLVA schemes with different combination of VNTR loci were tested, and two of them were suitable for isolates from China: scheme 2 with six loci was found to separate ST into finer resolution, and scheme 3 with five loci can be used to identify outbreak-associated isolates from the same outbreak that caused by N. meningitidis serogroup C ST-4821.

وصف الملف: electronic resource

العلاقة: http://europepmc.org/articles/PMC4300189?pdf=renderTest; https://doaj.org/toc/1932-6203Test

الوصول الحر: https://doaj.org/article/5074b93811af410e928d4d9231eca417Test

المؤلفون: Xiaoying Shan, Ji Zhang, Haijian Zhou, Bingqing Zhu, Li Xu, Zhujun Shao, Baofa Jiang

المصدر: PLoS ONE, Vol 9, Iss 11, p e111866 (2014)

مصطلحات موضوعية: Medicine, Science

الوصف: Neisseria meningitidis sequence type (ST)-4821 was first reported in China in 2003, and a new hyper-virulent lineage has been designated as the ST-4821 complex. A large number of N. meningitidis ST-4821 strains have been identified in China since 2003; however, the microevolution characteristics of this complex are unclear. Different combinations of variable number of tandem repeats (VNTR) loci were used in multiple-locus VNTR analysis (MLVA) to analyze 118 N. meningitidis serogroup C ST-4821 strains isolated from seventeen provinces between 2003 and 2012. Additionally, MLVA with five VNTR loci was performed due to its high discriminatory power. One hundred and eighteen isolates were found to comprise 112 subtypes based on MLVA, and 16 outbreak-associated strains were clustered into one group. These data indicate a high level of diversity for N. meningitidis ST-4821 due to microevolution in the last decade. In addition, the results revealed high similarity between isolates from the same geographic origins, which is helpful when monitoring the spread of N. meningitidis serogroup C ST-4821 and will provide valuable information for the control and prevention of bacterial meningitis in China.

وصف الملف: electronic resource

العلاقة: http://europepmc.org/articles/PMC4222954?pdf=renderTest; https://doaj.org/toc/1932-6203Test

الوصول الحر: https://doaj.org/article/8f6022d31148466ca411e45f0f2854bcTest