المؤلفون: Pasquale Angrisani, Roberto Cuomo, Domenico Memoli, Giuseppina Liguori, Carlo Baldi, Alessia Caleo, Antonio D'Antonio, Basilio Angrisani

المصدر: Rare Tumors, Vol 3, Iss 1, Pp e10-e10 (2011)

مصطلحات موضوعية: pleomorphic liposarcoma, FISH analysis, epithelioid variant, immunohistochemistry, differential diagnosis, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: We report a case of epithelioid variant of pleomorphic liposarcoma (EPL) found in the the infrapatellar fat pad of Hoffa of a 31-year old male. Histologically, the predominant population was formed by epithelioid cells with eosinophilic or clear cytoplasm admixed with rare pleomorphic lipoblasts. The immunohistochemical panel was not helpful in the diagnosis. FISH analysis using the locus-specific indicator CHOP (12q13) dual color break apart was applied to representative formalin-fixed, paraffin-embedded tissue sections. The result of FISH indicated a rearranged CHOP (DDIT3) gene and confirmed the diagnosis of EPL. The EPL should be differentiated from a metastatic carcinoma or other type of sarcoma. In these cases a clinicopathological correlation and an exhaustive sampling of the specimen for demonstration of lipogenic areas or pleomorphic lipoblasts is always necessary. FISH with demonstration of CHOP gene rearrangement is useful in providing specific ancillary information for the difficult differential diagnosis of this case.

وصف الملف: electronic resource

العلاقة: http://www.pagepress.org/journals/index.php/rt/article/view/2249Test; https://doaj.org/toc/2036-3605Test; https://doaj.org/toc/2036-3613Test

الوصول الحر: https://doaj.org/article/1a00e97fc780451ba26d994d7caef6f2Test

المؤلفون: Giulio C. Spagnoli, Luigi Terracciano, Luigi Tornillo, Markus Zuber, Giuseppe Sconocchia, Paul Zajac, Chantal Mengus, Manuele G. Muraro, Michel Adamina, Urs von Holzen, Daniel Oertli, Martin Bolli, Raffaele Rosso, Raoul A. Droeser, Basilio Angrisani, Elisabetta Padovan, Xaver Huber, Eleonora Cremonesi, Francesca Amicarella, Christian Hirt, Giandomenica Iezzi, Serenella Eppenberger-Castori, Junyi Han, Christian A. Nebiker

الوصف: PDF - 480K, CX3CL1/fractalkine gene and protein expression in healthy colon mucosa, CRC and established cell lines.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56bd66cd0fa587d5d6ea72b377f4d270Test
https://doi.org/10.1158/1078-0432.22451705Test

المؤلفون: Giulio C. Spagnoli, Luigi Terracciano, Luigi Tornillo, Markus Zuber, Giuseppe Sconocchia, Paul Zajac, Chantal Mengus, Manuele G. Muraro, Michel Adamina, Urs von Holzen, Daniel Oertli, Martin Bolli, Raffaele Rosso, Raoul A. Droeser, Basilio Angrisani, Elisabetta Padovan, Xaver Huber, Eleonora Cremonesi, Francesca Amicarella, Christian Hirt, Giandomenica Iezzi, Serenella Eppenberger-Castori, Junyi Han, Christian A. Nebiker

الوصف: Purpose: Colorectal cancer infiltration by CD16+ myeloid cells correlates with improved prognosis. We addressed mechanistic clues and gene and protein expression of cytokines potentially associated with macrophage polarization.Experimental Design: GM-CSF or M-CSF–stimulated peripheral blood CD14+ cells from healthy donors were cocultured with colorectal cancer cells. Tumor cell proliferation was assessed by 3H-thymidine incorporation. Expression of cytokine genes in colorectal cancer and autologous healthy mucosa was tested by quantitative, real-time PCR. A tumor microarray (TMA) including >1,200 colorectal cancer specimens was stained with GM-CSF- and M-CSF–specific antibodies. Clinicopathological features and overall survival were analyzed.Results: GM-CSF induced CD16 expression in 66% ± 8% of monocytes, as compared with 28% ± 1% in cells stimulated by M-CSF (P = 0.011). GM-CSF but not M-CSF–stimulated macrophages significantly (P < 0.02) inhibited colorectal cancer cell proliferation. GM-CSF gene was expressed to significantly (n = 45, P < 0.0001) higher extents in colorectal cancer than in healthy mucosa, whereas M-CSF gene expression was similar in healthy mucosa and colorectal cancer. Accordingly, IL1β and IL23 genes, typically expressed by M1 macrophages, were expressed to significantly (P < 0.001) higher extents in colorectal cancer than in healthy mucosa. TMA staining revealed that GM-CSF production by tumor cells is associated with lower T stage (P = 0.02), “pushing” growth pattern (P = 0.004) and significantly (P = 0.0002) longer survival in mismatch-repair proficient colorectal cancer. Favorable prognostic effect of GM-CSF production by colorectal cancer cells was confirmed by multivariate analysis and was independent from CD16+ and CD8+ cell colorectal cancer infiltration. M-CSF expression had no significant prognostic relevance.Conclusions: GM-CSF production by tumor cells is an independent favorable prognostic factor in colorectal cancer. Clin Cancer Res; 20(12); 3094–106. ©2014 AACR.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8e6b516b4eb856019f073f749a40dc19Test
https://doi.org/10.1158/1078-0432.c.6522260.v1Test

المؤلفون: Giulio C. Spagnoli, Luigi Terracciano, Luigi Tornillo, Markus Zuber, Giuseppe Sconocchia, Paul Zajac, Chantal Mengus, Manuele G. Muraro, Michel Adamina, Urs von Holzen, Daniel Oertli, Martin Bolli, Raffaele Rosso, Raoul A. Droeser, Basilio Angrisani, Elisabetta Padovan, Xaver Huber, Eleonora Cremonesi, Francesca Amicarella, Christian Hirt, Giandomenica Iezzi, Serenella Eppenberger-Castori, Junyi Han, Christian A. Nebiker

الوصف: PDF - 216K, Notch Box plot of histoscore values in CRC TMA upon staining with GM-CSF and M-CSF specific reagents.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4848a7523e2ee3c07d64e901fa6c988aTest
https://doi.org/10.1158/1078-0432.22451708Test

المؤلفون: Stefano Laudati, Carmine De Bartolomeis, Ermanno D’Arco, Alberto Angrisani, Mariano Agrusta, Ersilio Trapanese, Basilio Angrisani, Giulio Tarro

المصدر: Journal of Advances in Medicine and Medical Research. :1-13

مصطلحات موضوعية: body regions, Pathology, medicine.medical_specialty, medicine.anatomical_structure, business.industry, Thyroid, Carcinoma, medicine, General Medicine, medicine.disease, business

الوصف: Background: This study investigated the utility of the Sonographic pattern "Black Ink" with BRAF mutation testing of thyroid fine-needle aspiration cytology specimens for the risk papillary thyroid microcarcinoma (PTMC). Case Presentation: We describe a case of a 41-year-old Caucasian woman affected by a ultrasonography “Black Ink” papillary thyroid microcarcinoma (PTMC) of the left lobe of the thyroid gland with very tiny size (Ø 0.4 cm). The characteristics, with the Diagnostic Imaging using Ultrasonography (US), Superb Micro-Vascular Imaging (SMI), fine-needle-aspiration cytology (FNAC) and mutation analysis are here discussed. There are more rare subtypes of thyroid cancer as papillary microcarcinoma "Black Ink" that even if small, are invasive and there is why the need to early diagnosis to avoid their aggressive behavior is needed. Nowadays, focusing on the size, the cut-off for non-occult tiny tumors has dropped to 0.3 cm. This value is of great relevance. Conclusion: Ultrasonography, FNAC and BRAF molecular study have proven to be the most sensitive diagnostic combination for the early detection of thyroid cancer. Despite the size of this micro-lesion, the Black Ink ultrasonographic pattern associated with malignant cytology at FNAC represents an important biological risk factor and could still be a predictor of the PTMC and risk for neck lymph node metastases.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::85a9ab32eb1fd7e8e2670ff741cd7682Test
https://doi.org/10.9734/jammr/2019/v30i130162Test

المؤلفون: Igor Shibaev, Karl Kingsley, R. Parvathy, B. I. Ituma, Abdulhameed Abdullah Alhabeeb, Hamoud A. Alsubaie, Yuriy Kuksa, Basilio Angrisani, O. Iyare, Kingsley Akaba, Esmat Abdel Ghaffar, Geetanjali Sharma, Shayan Sirat Maheen Anwar, Timothy Ekwere, S. O. Oyinloye, E. S. Yedak, Mariano Agrusta, Naseem Akhtar Qureshi, Gauri Chauhan, R. Gayathry, M. Beraia, Stefano Laudati, D. S. Anand Raja, Osama El Shabrawy, A. K. Bhardwaj, J. Oyebanji, M. P. Archana, Efiok Efiok, Douglas Bowen, Gazzaffi I. M. Ali, M. Yusuf, Ermanno D’Arco, Giulio Tarro, Pallab Mandal, C. Rajasekharan, Ushie Godwin, Kainat Baig, Marcus Inyama, Fatima Mubarak, G. Beraia, Olesja Isaikina, Saud M. Alsanad, Inderpreet Santok, C. O. Akpa, Aarti Sareen, Alberto Angrisani, Obinna Iheanacho, Carmine De Bartolomeis, Hogan Archibong, Ersilio Trapanese, Ekpeyong Bassey, Vineet Nair, Wafaa Abdel-Aal

مصطلحات موضوعية: Library science

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::2edb6bb81912ef98affed2a3329e8478Test
https://doi.org/10.9734/bpi/erms/v2Test

المؤلفون: Carmine De Bartolomeis, Ersilio Trapanese, Giulio Tarro, Basilio Angrisani

المصدر: Oncotarget

مصطلحات موضوعية: FNAC, medicine.medical_specialty, business.industry, Papillary Thyroid Microcarcinoma, Case Report, 030209 endocrinology & metabolism, ultrasonography, Black Ink, Normal thyroid, Stain, body regions, 03 medical and health sciences, 0302 clinical medicine, Oncology, 030220 oncology & carcinogenesis, Cytology, Medical imaging, Medicine, thyroid microcarcinoma, Radiology, Ultrasonography, business, ADF

الوصف: We report a case of a 58-year-old Caucasian woman affected by papillary thyroid microcarcinoma (PTMC) of the left-lobe of the gland with very small size (Ø 0.3 cm). The characteristics with the Diagnostic Imaging using Ultrasonography, ADF (Advanced Dynamic Flow), and fine-needle-aspiration cytology (FNAC) are discussed, comprising a very small micro-focus of radial shape, with markedly hypoechoic echostructure, irregular margins, supplemented by peripheral vessel formation. It acquires an image which appears similar to a brisk visualization of a dark ink stain in the normal thyroid weave. We call such a pattern “Black Ink” with ultrasonographic image and believe consistent with the infiltrating variant of papillary thyroid microcarcinoma if associated with malignant cytology after FNA.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87a0b3bba9abe907479757d16d3d9042Test
https://doi.org/10.18632/oncotarget.25621Test

المؤلفون: Giampiero Mottola, Anna Maria Cascone, Matteo Cavaliere, Basilio Angrisani, Gabriella Fiorillo, Fabrizio Volino, Maurizio Iemma

المساهمون: The Pennsylvania State University CiteSeerX Archives

المصدر: ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/35/c9/Cases_J_2008_Dec_1_1_365.tar.gz

الوصف: This is an Open Access article distributed under the terms of the Creative Commons Attribution License

وصف الملف: application/zip

العلاقة: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.358.5590Test

المؤلفون: Alessandro Puzziello, M. Forlenza, Angelo Massari, L. Valvano, Francesco Antonio Salzano, Basilio Angrisani, Pio Zeppa, Mario Vitale, Maria Siano, Alessia Caleo

المصدر: Cytopathology. 28:273-279

مصطلحات موضوعية: Male, Pathology, medicine.medical_specialty, Histology, Cytoplasmic inclusion, Biopsy, Fine-Needle, 030209 endocrinology & metabolism, Fine needle cytology, Parathyroid neoplasms, 2734, Pathology and Forensic Medicine, Diagnosis, Differential, Parathyroid Glands, 03 medical and health sciences, 0302 clinical medicine, Cytology, medicine, Carcinoma, Humans, Nuclear atypia, Atypical Adenoma, Parathyroid adenoma, business.industry, Parathyroid neoplasm, General Medicine, Middle Aged, biochemical phenomena, metabolism, and nutrition, medicine.disease, 030220 oncology & carcinogenesis, Female, business

الوصف: Background Fine needle cytology (FNC) of a parathyroid neoplasia (PN) is reliable, but needs to be confirmed by Parathormone (PTH) and Thyroglobulin (TG) immunoassay on needle washing or by immunocytochemistry (ICC) evaluation. The differentiation between parathyroid adenoma (PA), atypical adenoma (PAA) and carcinoma (PC) is difficult on histology or even impossible on FNC. The aim of this study was to evaluate possible cytological criteria to classify FNC-PN further. Methods Twenty-three FNC samples of PN and parathyroid cysts were rather then have been reviewed. The series includes 18 PNs, 4 cysts and 1 Thyr3B (histologically diagnosed as PA). Cytological features were: cellularity, patterns (follicular, solid or papillary), clear, oncocytic, isolated cells, nuclear atypia, cytoplasmic inclusions, nucleoli and mitoses. Data were compared with the histological controls. Results Seventeen PNs, 2 cysts and 1 Thyr3B FNC samples were histologically diagnosed as PA (16), PAA (2) and PC (2). Two cysts and 1 PN were not confirmed histologically. Cytological features and incidences were: high cellularity (1 PA, 1 PAA, 2 PCs), follicular (8 PAs, 1 PAA), solid (5 PAs, 1 PC), papillary pattern (1PA, 1 PAA, 1 PC), clear cells (4 PAs, 1 PAA, 2 PCs), oncocytic cells (6 PAs, 1 PAA, 2 PCs), isolated cells (5 PAs, 2 PAAs, 2 PCs), nuclear atypia (2 PAs, 1 PAA, 2 PCs), cytoplasmic inclusions (4 PAs, 2 PCs), nucleoli (2 PCs) and mitoses (2 PCs). Conclusion Evident nucleoli and mitoses may suggest the differentiation between PA and PC. However, further investigations are required to confirm these preliminary observations.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5abbc9cdc79b4f421d8cf3814152f9b1Test
https://doi.org/10.1111/cyt.12413Test

المؤلفون: Maria Siano, Alessandro Vatrella, Rosa Maria Di Crescenzo, Anna Maria Cascone, Francesco Antonio Salzano, Pio Zeppa, Carmine Selleri, Basilio Angrisani

المصدر: Aging Clinical and Experimental Research. 29:167-171

مصطلحات موضوعية: Male, Aging, Pathology, medicine.medical_specialty, Breast carcinoma, Fine-needle cytology, Follow-up, Cytodiagnosis, Biopsy, Fine-Needle, Breast Neoplasms, Sensitivity and Specificity, 03 medical and health sciences, 0302 clinical medicine, Cytology, Humans, Medicine, Postoperative Period, Contralateral breast, Neoplasm Metastasis, Mastectomy, Aged, business.industry, Geriatrics gerontology, Reproducibility of Results, Soft tissue, Middle Aged, Anatomical sites, Italy, 030220 oncology & carcinogenesis, Female, 030211 gastroenterology & hepatology, Radiology, Lymph, Neoplasm Recurrence, Local, Geriatrics and Gerontology, business, Follow-Up Studies

الوصف: The postoperative follow-up strategies for breast carcinoma (BC) utilize different procedures; the aim of this study was to investigate the role of fine-needle cytology (FNC) in the follow-up of BC patients. Two hundred sixty-six FNC samples from 190 BC patients have been reviewed. The target anatomical sites were 190 breast including 155 ipsilateral and 145 contralateral breast lesions and 76 extra-mammary nodules. Extra-mammary lesions included lymph nodes, thyroidal nodules, soft tissue lesions, (subcutaneous and sub-scars), salivary glands and deep located masses. Diagnostic distribution of the breast lesions was as follows: 51 positive, 15 indeterminate/suspicious, 119 negative and 5 inadequate. Positive cases included 43 ipsilateral and 8 contralateral BC, 9 BC in different quadrants from those of onset of the first BC. Sensitivity, specificity and accuracy have been 90, 91 and 90&, respectively. FNC, in a correct setting, is a reliable and effective method for the follow-up management of BC patients.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::59d919ed82fdc3182f22b3d3c7040d83Test
https://doi.org/10.1007/s40520-016-0645-zTest