المؤلفون: Aguilar, Stephanie Vargas, Aguilar, Oscar, Allan, Rhys, Amir, El Ad David, Angeli, Veronique, Artyomov, Maxim N, Asinovski, Natasha, Astarita, Jilian, Austen, K Frank, Bajpai, Geetika, Barrett, Nora, Baysoy, Alev, Benoist, Christophe, Bellemare-Pelletier, Angelique, Berg, Brad, Best, Adam, Bezman, Natalie, Blair, David, Blander, Julie M, Bogunovic, Milena, Brennan, Patrick, Brenner, Michael, Brown, Brian, Buechler, Matthew, Buenrostro, Jason, Casanova, Maria Acebes, Choi, Kyunghee, Chow, Andrew, Chudnovskiy, Aleksey, Cipoletta, Daniela, Cohen, Nadia, Collins, James J, Colonna, Marco, Cook, Alison, Costello, James, Cremasco, Viviana, Crowl, Ty, Crozat, Karine, Cruse, Richard, D'Angelo, June, Dalod, Marc, Davis, Scott, Demiralp, Cagatay, Deng, Tianda, Desai, Jigar V, Desland, Fiona, Dhainaut, Maxime, Ding, Jiarui, Doedens, Andrew, Dominguez, Claudia, Doran, Graeme, Dress, Regine, Dustin, Michael, Dwyer, Daniel, Dzhagalov, Ivan, Elpek, Kutlu, Ergun, Ayla, Ericson, Jeff, Esomonu, Eunice, Fairfax, Keke, Fletcher, Anne, Frascoli, Michela, Fuchs, Anja, Gainullina, Anastasiia, Gal-Oz, Shani, Gallagher, Michael, Gautier, Emmanuel, Gazit, Roi, Gibbings, Sophie, Giraud, Matthieu, Ginhoux, Florent, Goldrath, Ananda, Gotthardt, Dagmar, Gray, Daniel, Greter, Melanie, Grieshaber-Bouyer, Ricardo, Guilliams, Martin, Haidermota, Sara, Hardy, Randy, Hashimoto, Daigo, Helft, Julie, Hendricks, Deborah, Heng, Tracy, Hill, Jonathan, Hyatt, Gordon, Idoyaga, Juliana, Jakubzick, Claudia, Jarjoura, Jessica, Jepson, Daniel, Jia, Baosen, Jianu, Radu, Johanson, Tim, Jordan, Stefan, Jojic, Vladimir, Kamimura, Yosuke, Kana, Veronica, Kang, Joonsoo, Kapoor, Varun, Kenigsberg, Ephriam

المصدر: Nature Immunology. 21(7)

مصطلحات موضوعية: Animals, Gene Expression Regulation, Gene Regulatory Networks, Genomics, History, 21st Century, Immune System, Immunologic Techniques, Mice, Immunological Genome Project, Immunology

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/75r8v4btTest

المؤلفون: Gainullina, Anastasiia, Mogilenko, Denis A., Huang, Li-Hao, Todorov, Helena, Narang, Vipin, Kim, Ki-Wook, Yng, Lim Sheau, Kent, Andrew, Jia, Baosen, Seddu, Kumba, Krchma, Karen, Wu, Jun, Crozat, Karine, Tomasello, Elena, Dress, Regine, See, Peter, Scott, Charlotte, Gibbings, Sophie, Bajpai, Geetika, Desai, Jigar V., Maier, Barbara, This, Sebastien, Wang, Peter, Aguilar, Stephanie Vargas, Poupel, Lucie, Dussaud, Sebastien, Zhou, Tyng-An, Angeli, Veronique, Blander, J. Magarian, Choi, Kyunghee, Dalod, Marc, Dzhagalov, Ivan, Gautier, Emmanuel L., Jakubzick, Claudia, Lavine, Kory, Lionakis, Michail S., Paidassi, Helena, Sieweke, Michael H., Ginhoux, Florent, Guilliams, Martin, Benoist, Christophe, Merad, Miriam, Randolph, Gwendalyn J., Sergushichev, Alexey, Artyomov, Maxim N., on behalf of the ImmGen Consortium, missing

المصدر: CELL REPORTS ; ISSN: 2211-1247

مصطلحات موضوعية: Biology and Life Sciences, Medicine and Health Sciences, ARACHIDONIC-ACID METABOLISM, CELLS, STATE, MACROPHAGES, ACTIVATION, EXPRESSION, PATHWAYS, SUBSETS, DISEASE, IMMUNE

الوصف: The diversity of mononuclear phagocyte (MNP) subpopulations across tissues is one of the key physiological characteristics of the immune system. Here, we focus on understanding the metabolic variability of MNPs through metabolic network analysis applied to three large-scale transcriptional datasets: we introduce (1) an ImmGen MNP open-source dataset of 337 samples across 26 tissues; (2) a myeloid subset of ImmGen Phase I dataset (202 MNP samples); and (3) a myeloid mouse single-cell RNA sequencing (scRNA-seq) data -set (51,364 cells) assembled based on Tabula Muris Senis. To analyze such large-scale datasets, we develop a network-based computational approach, genes and metabolites (GAM) clustering, for unbiased identifica-tion of the key metabolic subnetworks based on transcriptional profiles. We define 9 metabolic subnetworks that encapsulate the metabolic differences within MNP from 38 different tissues. Obtained modules reveal that cholesterol synthesis appears particularly active within the migratory dendritic cells, while glutathione synthesis is essential for cysteinyl leukotriene production by peritoneal and lung macrophages.

وصف الملف: application/pdf

العلاقة: https://biblio.ugent.be/publication/01H8H2YV9JHWE4DX7WG0F6P77GTest; http://hdl.handle.net/1854/LU-01H8H2YV9JHWE4DX7WG0F6P77GTest; http://doi.org/10.1016/j.celrep.2023.112046Test; https://biblio.ugent.be/publication/01H8H2YV9JHWE4DX7WG0F6P77G/file/01H8H34RF2NG0DVE2BWSB055DXTest

الإتاحة: https://doi.org/10.1016/j.celrep.2023.112046Test
https://biblio.ugent.be/publication/01H8H2YV9JHWE4DX7WG0F6P77GTest
http://hdl.handle.net/1854/LU-01H8H2YV9JHWE4DX7WG0F6P77GTest
https://biblio.ugent.be/publication/01H8H2YV9JHWE4DX7WG0F6P77G/file/01H8H34RF2NG0DVE2BWSB055DXTest

المؤلفون: Grune, Jana, Bajpai, Geetika, Ocak, Pervin Tülin, Kaufmann, Eva, Mentkowski, Kyle, Pabel, Steffen, Kumowski, Nina, Pulous, Fadi E., Tran, Kim A., Rohde, David, Zhang, Shuang, Iwamoto, Yoshiko, Wojtkiewicz, Gregory R., Vinegoni, Claudio, Green, Ursula, Swirski, Filip K., Stone, James R., Lennerz, Jochen K., Divangahi, Maziar, Hulsmans, Maarten, Nahrendorf, Matthias

المصدر: Circulation (Ovid); July 2024, Vol. 150 Issue: 1 p49-61, 13p

المؤلفون: Bajpai, Geetika, Witcher, Mark, Anyanwu, Chinekwu, Shah, Aashit

المصدر: Neurology ; volume 102, issue 17_supplement_1 ; ISSN 0028-3878 1526-632X

الإتاحة: https://doi.org/10.1212/wnl.0000000000206606Test

المؤلفون: Koenig, Andrew L., Shchukina, Irina, Amrute, Junedh, Andhey, Prabhakar S., Zaitsev, Konstantin, Lai, Lulu, Bajpai, Geetika, Bredemeyer, Andrea, Smith, Gabriella, Jones, Cameran, Terrebonne, Emily, Rentschler, Stacey L., Artyomov, Maxim N., Lavine, Kory J.

المساهمون: American Heart Association, U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute, Burroughs Wellcome Fund, Fondation Leducq

المصدر: Nature Cardiovascular Research ; volume 1, issue 3, page 263-280 ; ISSN 2731-0590

الوصف: Heart failure represents a major cause of morbidity and mortality worldwide. Single-cell transcriptomics have revolutionized our understanding of cell composition and associated gene expression. Through integrated analysis of single-cell and single-nucleus RNA-sequencing data generated from 27 healthy donors and 18 individuals with dilated cardiomyopathy, here we define the cell composition of the healthy and failing human heart. We identify cell-specific transcriptional signatures associated with age and heart failure and reveal the emergence of disease-associated cell states. Notably, cardiomyocytes converge toward common disease-associated cell states, whereas fibroblasts and myeloid cells undergo dramatic diversification. Endothelial cells and pericytes display global transcriptional shifts without changes in cell complexity. Collectively, our findings provide a comprehensive analysis of the cellular and transcriptomic landscape of human heart failure, identify cell type-specific transcriptional programs and disease-associated cell states and establish a valuable resource for the investigation of human heart failure.

الإتاحة: https://doi.org/10.1038/s44161-022-00028-6Test
https://www.nature.com/articles/s44161-022-00028-6.pdfTest
https://www.nature.com/articles/s44161-022-00028-6Test

المؤلفون: Wong, Nicole R., Mohan, Jay, Kopecky, Benjamin J., Guo, Shuchi, Du, Lixia, Leid, Jamison, Feng, Guoshuai, Lokshina, Inessa, Dmytrenko, Oleksandr, Luehmann, Hannah, Bajpai, Geetika, Ewald, Laura, Bell, Lauren, Patel, Nikhil, Bredemeyer, Andrea, Weinheimer, Carla J., Nigro, Jessica M., Kovacs, Attila, Morimoto, Sachio, Bayguinov, Peter O., Fisher, Max.R., Stump, W. Tom, Greenberg, Michael, Fitzpatrick, James A.J., Epelman, Slava, Kreisel, Daniel, Sah, Rajan, Liu, Yongjian, Hu, Hongzhen, Lavine, Kory J.

المصدر: Immunity ; volume 54, issue 9, page 2072-2088.e7 ; ISSN 1074-7613

الإتاحة: https://doi.org/10.1016/j.immuni.2021.07.003Test
https://api.elsevier.com/content/article/PII:S1074761321002892?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S1074761321002892?httpAccept=text/plainTest

المؤلفون: Bajpai, Geetika, Nahrendorf, Matthias

المساهمون: MASSACHUSETTS GENERAL HOSPITAL

المصدر: Immunity ; volume 54, issue 6, page 1110-1122 ; ISSN 1074-7613

مصطلحات موضوعية: Infectious Diseases, Immunology, Immunology and Allergy

الإتاحة: https://doi.org/10.1016/j.immuni.2021.05.011Test
https://api.elsevier.com/content/article/PII:S107476132100217X?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S107476132100217X?httpAccept=text/plainTest

المؤلفون: Bajpai, Geetika, Bredemeyer, Andrea, Li, Wenjun, Zaitsev, Konstantin, Koenig, Andrew L., Lokshina, Inessa, Mohan, Jayaram, Ivey, Brooke, Hsiao, His-Min, Weinheimer, Carla, Kovacs, Attila, Epelman, Slava, Artyomov, Maxim, Kreisel, Daniel, Lavine, Kory J.

المصدر: Circulation Research ; volume 124, issue 2, page 263-278 ; ISSN 0009-7330 1524-4571

الوصف: Rationale: Recent advancements have brought to light the origins, complexity, and functions of tissue-resident macrophages. However, in the context of tissue injury or disease, large numbers of monocytes infiltrate the heart and are thought to contribute to adverse remodeling and heart failure pathogenesis. Little is understood about the diversity of monocytes and monocyte-derived macrophages recruited to the heart after myocardial injury, including the mechanisms that regulate monocyte recruitment and fate specification. Objective: We sought to test the hypothesis that distinct subsets of tissue-resident CCR2− (C-C chemokine receptor 2) and CCR2+ macrophages orchestrate monocyte recruitment and fate specification after myocardial injury. Methods and Results: We reveal that in numerous mouse models of cardiomyocyte cell death (permanent myocardial infarction, reperfused myocardial infarction, and diphtheria toxin cardiomyocyte ablation), there is a shift in macrophage ontogeny whereby tissue-resident macrophages are predominately replaced by infiltrating monocytes and monocyte-derived macrophages. Using syngeneic cardiac transplantation to model ischemia-reperfusion injury and distinguish tissue-resident from recruited cell populations in combination with intravital 2-photon microscopy, we demonstrate that monocyte recruitment is differentially orchestrated by distinct subsets of tissue-resident cardiac macrophages. Tissue-resident CCR2+ macrophages promote monocyte recruitment through an MYD88 (myeloid differentiation primary response 88)-dependent mechanism that results in release of MCPs (monocyte chemoattractant proteins) and monocyte mobilization. In contrast, tissue-resident CCR2− macrophages inhibit monocyte recruitment. Using CD (cluster of differentiation) 169-DTR (diphtheria toxin receptor) and CCR2-DTR mice, we further show that selective depletion of either tissue-resident CCR2− or CCR2+ macrophages before myocardial infarction results in divergent effects on left ventricular function, myocardial ...

الإتاحة: https://doi.org/10.1161/circresaha.118.314028Test

المؤلفون: Heo, Gyu Seong, Kopecky, Benjamin, Sultan, Deborah, Ou, Monica, Feng, Guoshuai, Bajpai, Geetika, Zhang, Xiaohui, Luehmann, Hannah, Detering, Lisa, Su, Yi, Leuschner, Florian, Combadière, Christophe, Kreisel, Daniel, Gropler, Robert J., Brody, Steven L., Liu, Yongjian, Lavine, Kory J.

المصدر: Circulation Research ; volume 124, issue 6, page 881-890 ; ISSN 0009-7330 1524-4571

الوصف: Rationale: Paradigm shifting studies have revealed that the heart contains functionally diverse populations of macrophages derived from distinct embryonic and adult hematopoietic progenitors. Under steady-state conditions, the heart is largely populated by CCR2− (C-C chemokine receptor type 2) macrophages of embryonic descent. After tissue injury, a dramatic shift in macrophage composition occurs whereby CCR2+ monocytes are recruited to the heart and differentiate into inflammatory CCR2+ macrophages that contribute to heart failure progression. Currently, there are no techniques to noninvasively detect CCR2+ monocyte recruitment into the heart and thus identify patients who may be candidates for immunomodulatory therapy. Objective: To develop a noninvasive molecular imaging strategy with high sensitivity and specificity to visualize inflammatory monocyte and macrophage accumulation in the heart. Methods and Results: We synthesized and tested the performance of a positron emission tomography radiotracer ( 68 Ga-DOTA [1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid]-ECL1i [extracellular loop 1 inverso]) that allosterically binds to CCR2. In naive mice, the radiotracer was quickly cleared from the blood and displayed minimal retention in major organs. In contrast, biodistribution and positron emission tomography demonstrated strong myocardial tracer uptake in 2 models of cardiac injury (diphtheria toxin induced cardiomyocyte ablation and reperfused myocardial infarction). 68 Ga-DOTA-ECL1i signal localized to sites of tissue injury and was independent of blood pool activity as assessed by quantitative positron emission tomography and ex vivo autoradiography. 68 Ga-DOTA-ECL1i uptake was associated with CCR2+ monocyte and CCR2+ macrophage infiltration into the heart and was abrogated in CCR2 −/ − mice, demonstrating target specificity. Autoradiography demonstrated that 68 Ga-DOTA-ECL1i specifically binds human heart failure specimens and with signal intensity associated with CCR2+ macrophage abundance. ...

الإتاحة: https://doi.org/10.1161/circresaha.118.314030Test

المؤلفون: Wang, Yuhua, Du, Qiumei, Pan, Hongjie, Bajpai, Geetika, Liu, Tao, Noon, Jason, Lu, Yao, He, Jinqiu, Li, Yan, Chen, Qingfang, Wang, Yongqiang, Jia, Xingxing, Chen, Fei, Wang, Youhong, Rong, Yiping, He, Yun, Zhao, Joe, Bao, Musheng

المصدر: Regular and Young Investigator Award Abstracts

الإتاحة: https://doi.org/10.1136/jitc-2022-sitc2022.0477Test