المؤلفون: Chiavaroli, Annalisa, Masciulli, Fabrizio, Ingallina, Cinzia, Mannina, Luisa, Loreta Libero, Maria, Di Simone, Simonetta Cristina, Acquaviva, Alessandra, Nilofar, Recinella, Lucia, Leone, Sheila, Brunetti, Luigi, Carradori, Simone, Cantò, Luca, Orlando, Giustino, Zengin, Gokhan, Ibrahim Uba, Abdullah, Cakilcioğlu, Ugur, Mukemre, Muzaffer, Elkiran, Omer, Di Vito, Maura, Menghini, Luigi, Ferrante, Claudio

المساهمون: Ministero dell’Istruzione, dell’Università e della Ricerca, Università degli Studi G. d'Annunzio Chieti - Pescara, MIUR

المصدر: Food Research International ; volume 175, page 113654 ; ISSN 0963-9969

مصطلحات موضوعية: Food Science

الإتاحة: https://doi.org/10.1016/j.foodres.2023.113654Test
https://api.elsevier.com/content/article/PII:S0963996923012024?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0963996923012024?httpAccept=text/plainTest

المؤلفون: Recinella, Lucia, De Filippis, Barbara, Libero, Maria Loreta, Ammazzalorso, Alessandra, Chiavaroli, Annalisa, Orlando, Giustino, Ferrante, Claudio, Giampietro, Letizia, Veschi, Serena, Cama, Alessandro, Mannino, Federica, Gasparo, Irene, Bitto, Alessandra, Amoroso, Rosa, Brunetti, Luigi, Leone, Sheila

المساهمون: Recinella, Lucia, De Filippis, Barbara, Libero, Maria Loreta, Ammazzalorso, Alessandra, Chiavaroli, Annalisa, Orlando, Giustino, Ferrante, Claudio, Giampietro, Letizia, Veschi, Serena, Cama, Alessandro, Mannino, Federica, Gasparo, Irene, Bitto, Alessandra, Amoroso, Rosa, Brunetti, Luigi, Leone, Sheila

مصطلحات موضوعية: PPAR modulator, UCP1, adipose tissue, obesity

الوصف: Activation of peroxisome proliferator-activated receptors (PPARs) not only regulates multiple metabolic pathways, but mediates various biological effects related to inflammation and oxidative stress. We investigated the effects of four new PPAR ligands containing a fibrate scaffold-the PPAR agonists (1a (αEC50 1.0 μM) and 1b (γEC50 0.012 μM)) and antagonists (2a (αIC50 6.5 μM) and 2b (αIC50 0.98 μM, with a weak antagonist activity on γ isoform))-on proinflammatory and oxidative stress biomarkers. The PPAR ligands 1a-b and 2a-b (0.1-10 μM) were tested on isolated liver specimens treated with lipopolysaccharide (LPS), and the levels of lactate dehydrogenase (LDH), prostaglandin (PG) E2, and 8-iso-PGF2α were measured. The effects of these compounds on the gene expression of the adipose tissue markers of browning, PPARα, and PPARγ, in white adipocytes, were evaluated as well. We found a significant reduction in LPS-induced LDH, PGE2, and 8-iso-PGF2α levels after 1a treatment. On the other hand, 1b decreased LPS-induced LDH activity. Compared to the control, 1a stimulated uncoupling protein 1 (UCP1), PR-(PRD1-BF1-RIZ1 homologous) domain containing 16 (PRDM16), deiodinase type II (DIO2), and PPARα and PPARγ gene expression, in 3T3-L1 cells. Similarly, 1b increased UCP1, DIO2, and PPARγ gene expression. 2a-b caused a reduction in the gene expression of UCP1, PRDM16, and DIO2 when tested at 10 μM. In addition, 2a-b significantly decreased PPARα gene expression. A significant reduction in PPARγ gene expression was also found after 2b treatment. The novel PPARα agonist 1a might be a promising lead compound and represents a valuable pharmacological tool for further assessment. The PPARγ agonist 1b could play a minor role in the regulation of inflammatory pathways.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/36986448; volume:16; issue:3; firstpage:346; journal:PHARMACEUTICALS; https://hdl.handle.net/11564/802657Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85152449092; https://www.mdpi.com/1424-8247/16/3/346Test

الإتاحة: https://doi.org/10.3390/ph16030346Test
https://hdl.handle.net/11564/802657Test
https://www.mdpi.com/1424-8247/16/3/346Test

المؤلفون: Recinella, Lucia, Libero, Maria Loreta, Veschi, Serena, Piro, Anna, Marconi, Guya Diletta, Diomede, Francesca, Chiavaroli, Annalisa, Orlando, Giustino, Ferrante, Claudio, Florio, Rosalba, Lamolinara, Alessia, Cai, Renzhi, Sha, Wei, Schally, Andrew V, Salvatori, Roberto, Brunetti, Luigi, Leone, Sheila

المساهمون: Recinella, Lucia, Libero, Maria Loreta, Veschi, Serena, Piro, Anna, Marconi, Guya Diletta, Diomede, Francesca, Chiavaroli, Annalisa, Orlando, Giustino, Ferrante, Claudio, Florio, Rosalba, Lamolinara, Alessia, Cai, Renzhi, Sha, Wei, Schally, Andrew V, Salvatori, Roberto, Brunetti, Luigi, Leone, Sheila

مصطلحات موضوعية: GHRH antagonism, GHRH deficiency, anxiety, depression, mood disorders

الوصف: Growth hormone (GH)-releasing hormone (GHRH) has been suggested to play a crucial role in brain function. We aimed to further investigate the effects of a novel GHRH antagonist of the Miami (MIA) series, MIA-602, on emotional disorders and explore the relationships between the endocrine system and mood disorders. In this context, the effects induced by MIA-602 were also analyzed in comparison to vehicle-treated mice with GH deficiency due to generalized ablation of the GHRH gene (GHRH knock out (GHRHKO)). We show that the chronic subcutaneous administration of MIA-602 to wild type (+/+) mice, as well as generalized ablation of the GHRH gene, is associated with anxiolytic and antidepressant behavior. Moreover, immunohistochemical and Western blot analyses suggested an evident activation of Nrf2, HO1, and NQO1 in the prefrontal cortex of both +/+ mice treated with MIA-602 (+/+ MIA-602) and homozygous GHRHKO (-/- control) animals. Finally, we also found significantly decreased COX-2, iNOS, NFkB, and TNF-α gene expressions, as well as increased P-AKT and AKT levels in +/+ MIA-602 and -/- control animals compared to +/+ mice treated with vehicle (+/+ control). We hypothesize that the generalized ablation of the GHRH gene leads to a dysregulation of neural pathways, which is mimicked by GHRH antagonist treatment.

وصف الملف: ELETTRONICO

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/37998350; volume:12; issue:22; firstpage:1; lastpage:13; numberofpages:13; journal:CELLS; https://hdl.handle.net/11564/821371Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85177776746; https://www.mdpi.com/2073-4409/12/22/2615Test

الإتاحة: https://doi.org/10.3390/cells12222615Test
https://hdl.handle.net/11564/821371Test
https://www.mdpi.com/2073-4409/12/22/2615Test

المؤلفون: Deshpande, Kiran, Lange, Keith R., Stone, William B., Yohn, Christine, Schlesinger, Naomi, Kagan, Leonid, Auguste, Albert J., Firestein, Bonnie L., Brunetti, Luigi

مصطلحات موضوعية: COVID-19, drug transporters, drug-metabolizing enzymes, hACE2, SARS-CoV-2

الوصف: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and the resulting Coronavirus disease 2019 emerged in late 2019 and is responsible for significant morbidity and mortality worldwide. A hallmark of severe COVID-19 is exaggerated systemic inflammation, regarded as a "cytokine storm," which contributes to the damage of various organs, primarily the lungs. The inflammation associated with some viral illnesses is known to alter the expression of drug-metabolizing enzymes and transporters. These alterations can lead to modifications in drug exposure and the processing of various endogenous compounds. Here, we provide evidence to support changes in the mitochondrial ribonucleic acid expression of a subset of drug transporters (84 transporters) in the liver, kidneys, and lungs and metabolizing enzymes (84 enzymes) in the liver in a humanized angiotensin-converting enzyme 2 receptor mouse model. Specifically, three drug transporters (Abca3, Slc7a8, Tap1) and the pro-inflammatory cytokine IL-6 were upregulated in the lungs of SARS-CoV-2 infected mice. We also found significant downregulation of drug transporters responsible for the movement of xenobiotics in the liver and kidney. Additionally, expression of cytochrome P-450 2f2 which is known to metabolize some pulmonary toxicants, was significantly decreased in the liver of infected mice. The significance of these findings requires further exploration. Our results suggest that further research should emphasize altered drug disposition when investigating therapeutic compounds, whether re-purposed or new chemical entities, in other animal models and ultimately in individuals infected with SARS-CoV-2. Moreover, the influence and impact of these changes on the processing of endogenous compounds also require further investigation. ; IDCare ; Published version ; IDCare

وصف الملف: application/pdf

العلاقة: e01071; http://hdl.handle.net/10919/115480Test; https://doi.org/10.1002/prp2.1071Test; 11

الإتاحة: https://doi.org/10.1002/prp2.1071Test
http://hdl.handle.net/10919/115480Test

المؤلفون: Acquaviva, Alessandra, Di Simone, Simonetta Cristina, Nilofar, Abdelhakim, Bouyahya, Abdelhakim, Zengin, Gokhan, Recinella, Lucia, Leone, Sheila, Brunetti, Luigi, Uba, Abdullahi Ibrahim, Guler, Osman, Balos, Maruf, Cakilcioglu, Ugur, Menghini, Luigi, Ferrante, Claudio, Orlando, Giustino, Libero, Maria Loreta, Chiavaroli, Annalisa

مصطلحات موضوعية: Anti-Inflammatory, Antioxidant, Nepeta Italica, Rosmarinic Acid

الوصف: Plants from the Nepeta genus have been proved to possess different pharmacological properties, among which are antimicrobial, antioxidant, anti-inflammatory, analgesic, and cytotoxic effects. Nepeta italica is a medicinal plant traditionally used for its analgesic effects, and in the present study, the phytochemical composition and biological effects of hexane, dichloromethane (DCM), ethyl acetate (EA), ethanol, ethanol-water, and water extracts of the aerial parts were investigated for determining phenolic composition, antioxidant effects, and anti-inflammatory effects in isolated mouse colon specimens exposed to lipopolysaccharide (LPS). Polar extracts were the richest in terms of phenolic compounds, especially rosmarinic acid. In parallel, ethanol, ethanol-water, and water extracts were also the most effective as scavenging/reducing and enzyme inhibition agents, especially towards cholinesterases and & alpha;-glucosidase, and in inhibiting the LPS-induced cyclooxygenase-2 (COX-2) and tumor necrosis factor & alpha; (TNF & alpha;) gene expression in mouse colon. This poses the basis for future in vivo investigations for confirming the protective effects of polar extracts of N. italica against inflammatory bowel diseases.

وصف الملف: application/pdf

العلاقة: PLANTS-BASEL; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; Acquaviva, A., Di Simone, S. C., Nilofar, Bouyahya, A., Zengin, G., Recinella, L., . & Chiavaroli, A. (2023). Screening for chemical characterization and pharmacological properties of different extracts from Nepeta italica. Plants, 12(15), 2785.; https://doi.org/10.3390/plants12152785Test; https://hdl.handle.net/20.500.12294/3967Test; 12; 15; Q1; WOS:001045572600001; 2-s2.0-85167409232

الإتاحة: https://doi.org/10.3390/plants12152785Test
https://doi.org/20.500.12294/3967Test
https://hdl.handle.net/20.500.12294/3967Test

المؤلفون: Schnipper, Jeffrey L, Reyes Nieva, Harry, Yoon, Catherine, Mallouk, Meghan, Mixon, Amanda S, Rennke, Stephanie, Chu, Eugene S, Mueller, Stephanie K, Smith, G Randy, Williams, Mark V, Wetterneck, Tosha B, Stein, Jason, Dalal, Anuj K, Labonville, Stephanie, Sridharan, Anirudh, Stolldorf, Deonni P, Orav, Endel John, Gresham, Marcus, Goldstein, Jenna, Platt, Sara, Nyenpan, Christopher Tugbéh, Howell, Eric, Kripalani, Sunil, MARQUIS2 Site Leaders for the MARQUIS2 Study Group, MARQUIS2 Site Leaders, Sen, Sanchita, SamerBadr, Michelle Murphy, Vasilopoulos, Corrie, Vlasimsky, Tara, Roussel, Christine, Arole, Olugbenga, Berescu, Loredana Diana, Arifuddowla, Arif, Main, Hattie, Pickle, Susan, Singleton, Cristy, Asplund, Brenda, Delrue, Andrea, Forgione, Andrea, Shipman, Colleen, Brunetti, Luigi, Ahmed, Hina, Gonzales, Adrian, MithuMolla, Sarah Bojerek, Nguyen, Andrea, El-Kareh, Robert

مصطلحات موضوعية: Original research

الوصف: Background The second Multicenter Medication Reconciliation Quality Improvement Study demonstrated a marked reduction in medication discrepancies per patient. The aim of the current analysis was to determine the association of patient exposure to each system-level intervention and receipt of each patient-level intervention on these results. Methods This study was conducted at 17 North American Hospitals, the study period was 18 months per site, and sites typically adopted interventions after 2–5 months of preintervention data collection. We conducted an on-treatment analysis (ie, an evaluation of outcomes based on patient exposure) of system-level interventions, both at the category level and at the individual component level, based on monthly surveys of implementation site leads at each site (response rate 65%). We then conducted a similar analysis of patient-level interventions, as determined by study pharmacist review of documented activities in the medical record. We analysed the association of each intervention on the adjusted number of medication discrepancies per patient in admission and discharge orders, based on a random sample of up to 22 patients per month per site, using mixed-effects Poisson regression with hospital site as a random effect. We then used a generalised linear mixed-effects model (GLMM) decision tree to determine which patient-level interventions explained the most variance in discrepancy rates. Results Among 4947 patients, patient exposure to seven of the eight system-level component categories was associated with modest but significant reductions in discrepancy rates (adjusted rate ratios (ARR) 0.75–0.97), as were 15 of the 17 individual system-level intervention components, including hiring, reallocating and training personnel to take a best possible medication history (BPMH) and training personnel to perform discharge medication reconciliation and patient counselling. Receipt of five of seven patient-level interventions was independently associated with large reductions in ...

وصف الملف: text/html

العلاقة: http://qualitysafety.bmj.com/cgi/content/short/32/8/457Test; http://dx.doi.org/10.1136/bmjqs-2022-014806Test

الإتاحة: https://doi.org/10.1136/bmjqs-2022-014806Test
http://qualitysafety.bmj.com/cgi/content/short/32/8/457Test

المؤلفون: Brunetti, Luigi, Wang, Lujing, Wassef, Andrew, Gong, Yongjia, Brinker, Anita, Buckley, Brian, Lipsky, Peter E., Ondar, Patricia, Poiani, George, Zhao, Liping, Kong, Ah‐Ng, Schlesinger, Naomi

المصدر: Molecular Nutrition & Food Research ; volume 67, issue 9 ; ISSN 1613-4125 1613-4133

الوصف: Scope Tart cherries (TCs) contain high levels of anthocyanins that exert potent antioxidant and antiinflammatory effects and potentially benefit individuals with gout. Methods and results This study aims to quantitate the major anthocyanins in TC Juice Concentrate (TCJC) and identify the pharmacokinetic (PK) and pharmacodynamic (PD) parameters of the major anthocyanin cyanidin‐3‐glucosylrutinoside (C3GR). A PK‐PD study enrolling human subjects with a history of gout is performed. Subjects are randomized to receive either 60 or 120 mL of TCJC. Anthocyanins are quantitated using liquid chromatography‐mass spectroscopy (LCMS). Antioxidant and antiinflammatory mRNA expression is measured using real‐time qPCR before and after the administration of TCJC. A population PK model (popPK) is fit to the experimental data, and an indirect PD model (IDR) is constructed in Monolix. Conclusion Of the bioavailable anthocyanins, C3GR achieves the highest plasma concentration in a dose‐dependent manner. A popPK predicts anthocyanin exposure, and an IDR produces reasonable approximations of PD effects.

الإتاحة: https://doi.org/10.1002/mnfr.202200550Test

المؤلفون: Chiavaroli, Annalisa, Di Simone, Simonetta Cristina, Acquaviva, Alessandra, Nilofar, Nilofar, Libero, Maria Loreta, Brunetti, Luigi, Recinella, Lucia, Leone, Sheila, Orlando, Giustino, Zengin, Gokhan, Di Vito, Maura, Menghini, Luigi, Ferrante, Claudio

المصدر: Chemistry & Biodiversity; May2024, Vol. 21 Issue 5, p1-9, 9p

المؤلفون: Recinella, Lucia, Chiavaroli, Annalisa, Veschi, Serena, Di Valerio, Valentina, Lattanzio, Rossano, Orlando, Giustino, Ferrante, Claudio, Gesmundo, Iacopo, Granata, Riccarda, Cai, Renzhi, Sha, Wei, Schally, Andrew V, Brunetti, Luigi, Leone, Sheila

المساهمون: Recinella, Lucia, Chiavaroli, Annalisa, Veschi, Serena, Di Valerio, Valentina, Lattanzio, Rossano, Orlando, Giustino, Ferrante, Claudio, Gesmundo, Iacopo, Granata, Riccarda, Cai, Renzhi, Sha, Wei, Schally, Andrew V, Brunetti, Luigi, Leone, Sheila

مصطلحات موضوعية: Apoptotic pathway, Colorectal cancer, Growth hormone-releasing hormone antagonist, Inflammation, Oncogenic pathway, Oxidative stre, Animal, Apoptosi, Carcinogenesi, Cell Proliferation, Colorectal Neoplasm, Down-Regulation, Growth Hormone-Releasing Hormone, Inflammation Mediator, Male, Mice, Inbred C57BL, Random Allocation, Up-Regulation

الوصف: Colorectal cancer (CRC) is an aggressive tumor in which new treatment options deliver negative results on cure rates and long-term survival. The anticancer effects of growth hormone-releasing hormone (GHRH) antagonists have been reported in various experimental tumors, but their activity in CRC is unknown. In the present study, we demonstrated that chronic treatment with GHRH antagonist of MIAMI class, MIA-690, promoted survival and gradually blunted tumor progression in experimentally induced colitis-associated cancer in mice, paralleled by reduced inflammation in colon tissue. In particular, MIA-690 improved disease activity index score, and reduced loss of weight and mortality, by improving the survival rates, compared with vehicle-treated group. MIA-690 was also found to reduce various inflammatory and oxidative markers, such as serotonin, prostaglandin (PG)E2 and 8-iso-PGF2α levels, as well as COX-2, iNOS, TNF-α, IL-6 and NF-kB gene expression. Moreover, MIA-690 inhibited the protein expression of c-Myc, P-AKT and Bcl-2 and upregulated p53 protein expression. In conclusion, we showed that MIA-690 suppresses CRC progression and growth by reducing inflammatory and oxidative markers and modulating apoptotic and oncogenic pathways. Further investigations are required for translating these findings into the clinics.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/34923341; volume:146; issue:112554; firstpage:1; lastpage:11; numberofpages:11; journal:BIOMÉDECINE & PHARMACOTHÉRAPIE; http://hdl.handle.net/2318/1870424Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85121268310; https://www.sciencedirect.com/science/article/pii/S075333222101341X?via=ihubTest

الإتاحة: https://doi.org/10.1016/j.biopha.2021.112554Test
http://hdl.handle.net/2318/1870424Test
https://www.sciencedirect.com/science/article/pii/S075333222101341X?via=ihubTest

المؤلفون: Recinella, Lucia, Micheli, Laura, Chiavaroli, Annalisa, Libero, Maria Loreta, Orlando, Giustino, Menghini, Luigi, Acquaviva, Alessandra, Di Simone, Simonetta, Ferrante, Claudio, Ghelardini, Carla, Brunetti, Luigi, Leone, Sheila

المساهمون: Recinella, Lucia, Micheli, Laura, Chiavaroli, Annalisa, Libero, Maria Loreta, Orlando, Giustino, Menghini, Luigi, Acquaviva, Alessandra, Di Simone, Simonetta, Ferrante, Claudio, Ghelardini, Carla, Brunetti, Luigi, Leone, Sheila

مصطلحات موضوعية: Cultivar coratina, inflammation, osteoarthriti, hydroxytyrosol, pain

الوصف: MOMAST® GR25 is a polyphenolic granular complex from olive pressing juice with high total content in polyphenols. In this work, we evaluated the possible anti-inflammatory effects of MOMAST® GR25 in both acute and chronic inflammatory models. MOMAST® GR25 decreased the levels of prostaglandin (PG) E2 and 8-iso-PGF2α in isolated rat colon, liver, and heart specimens stimulated with lipopolysaccharide (LPS). In vivo, compared to controls, rats treated with MOMAST® GR25 (100 mg/kg to 1 g/kg) showed a significant reduction in both licking/biting time in the formalin test. In a rat model of osteoarthritis by monoiodoacetate (MIA) injection, MOMAST® GR25 showed pain-relieving properties when acutely administered, reducing mechanical hyperalgesia and spontaneous pain. Moreover, a repeated daily treatment with MOMAST® GR25 (300 mg/kg) fully counteracted osteoarticular pain without the development of tolerance to the antinociceptive effect. Taken together, our present findings showed that MOMAST® GR25 could represent a potential strategy for the treatment of inflammation and pain.

وصف الملف: ELETTRONICO

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/35406100; info:eu-repo/semantics/altIdentifier/wos/WOS:000780580900001; volume:14; issue:7; firstpage:1487; journal:NUTRIENTS; http://hdl.handle.net/11564/774351Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85127374446; https://www.mdpi.com/2072-6643/14/7/1487Test

الإتاحة: https://doi.org/10.3390/nu14071487Test
http://hdl.handle.net/11564/774351Test
https://www.mdpi.com/2072-6643/14/7/1487Test