المؤلفون: Avetisyan Gayane Akopovna

المصدر: Journal of Stress Physiology & Biochemistry, Vol 19, Iss 4, Pp 102-107 (2023)

مصطلحات موضوعية: abnormal conidial germination, induced oxidative stress, powdery mildew, wheat, Biochemistry, QD415-436

الوصف: Experiments for the study of symptoms of the powdery mildew pathogen on wheat leaves showed that induced oxidative stress caused changes in conidial germination and appressorial formation of the wheat powdery mildew fungus. The oxidative stress was brought about by treatment with hydrogen peroxide and 3-amino-1,2,4-triazole. It has been shown that prooxidants have a prominent role in regulating fungal development, leading to abnormal conidial germination, thus preventing the fungal penetration into plant cells. Treatment of wheat plants with 5 mM H2O2 and 4 mM 3-amino-1,2,4-triazole resulted in a significant reduction of powdery mildew disease severity compared to the control. In most cases, on samples of infected plant tissues there were anomalies in the elongation of germ tubes and globe-shaped appressoria. From the data which was obtained in this study, it can be concluded that the result of the interaction of powdery mildew fungus with wheat is affected by the increased generation of reactive oxygen species, leading to suppression or disruption of the pathological process.

وصف الملف: electronic resource

العلاقة: http://www.jspb.ru/issues/2023/N4/JSPB_2023_4_102-107.pdfTest; https://doaj.org/toc/1997-0838Test

الوصول الحر: https://doaj.org/article/f1dca32032774574a4760febb217ea78Test

المؤلفون: Gil, José Vicente, Miralles, Alberto, de las Heras, Sandra, Such, Esperanza, Avetisyan, Gayane, Díaz-González, Álvaro, Santiago, Marta, Fuentes, Carolina, Fernández, José María, Lloret, Pilar, Navarro, Irene, Montesinos, Pau, Llop, Marta, Barragán, Eva

المصدر: Frontiers in Molecular Biosciences ; volume 11 ; ISSN 2296-889X

مصطلحات موضوعية: Biochemistry, Genetics and Molecular Biology (miscellaneous), Molecular Biology, Biochemistry

الوصف: Introduction: Acute lymphoblastic leukemia (ALL) is a prevalent childhood cancer with high cure rate, but poses a significant medical challenge in adults and relapsed patients. Philadelphia-like acute lymphoblastic leukemia (Ph-like ALL) is a high-risk subtype, with approximately half of cases characterized by CRLF2 overexpression and frequent concomitant IKZF1 deletions. Methods: To address the need for efficient, rapid, and cost-effective detection of CRLF2 alterations, we developed a novel RT-qPCR technique combining SYBR Green and highresolution melting analysis on a single plate. Results: The method successfully identified CRLF2 expression, P2RY8::CRLF2 fusions, and CRLF2 and JAK2 variants, achieving a 100% sensitivity and specificity. Application of this method across 61 samples revealed that 24.59% exhibited CRLF2 overexpression, predominantly driven by IGH::CRLF2 (73.33%). High Resolution Melting analysis unveiled concurrent CRLF2 or JAK2 variants in 8.19% of samples, as well as a dynamic nature of CRLF2 alterations during disease progression. Discussion: Overall, this approach provides an accurate identification of CRLF2 alterations, enabling improved diagnostic and facilitating therapeutic decision-making.

الإتاحة: https://doi.org/10.3389/fmolb.2024.1362081Test

المؤلفون: Díaz González, Álvaro, Avetisyan, Gayane, Garcia-Ruiz, Cristian, Vicente Gil, José, Santiago, Marta, José Domínguez-García, Juan, Llop, Marta, Barragan, Eva, Leonor Senent Peris, Maria, DE LA Rubia, Javier, Cervera, José, Such, Esperanza

المصدر: HemaSphere ; volume 7, issue S3, page e8338249 ; ISSN 2572-9241

الإتاحة: https://doi.org/10.1097/01.hs9.0000976612.83382.49Test

المؤلفون: Babosha, Alexander V., Ryabchenko, Andrey S., Avetisyan, Gayane A., Avetisyan, Tamara V.

المصدر: Journal of Plant Pathology, 2020 Feb 01. 102(1), 103-111.

الوصول الحر: https://www.jstor.org/stable/48741358Test

المؤلفون: Avagimyan, Ashot, Fogacci, Federica, Pogosova, Nana, Kakturskiy, Lev, Jndoyan, Zinaida, Faggiano, Andrea, Bairamyan, Tamara, Agati, Luciano, Sattar, Yasar, Mkrchyan, Lusine, Avetisyan, Gayane, Ginosyan, Knarik, Aznauryan, Anahit, Sahakyan, Karmen, Trofimenko, Artem, Urazova, Olga, Mikhaleva, Liudmila, Vandysheva, Rositsa, Kogan, Eugenia, Demura, Tatiana, KC, Manish, Shafie, Davood, Nicola, Stefania, Brussino, Luisa, Cicero, Arrigo, Biondi-Zoccai, Giuseppe, Sarrafzadegan, Nizal

المصدر: Current Problems in Cardiology ; volume 49, issue 2, page 102230 ; ISSN 0146-2806

مصطلحات موضوعية: Cardiology and Cardiovascular Medicine, General Medicine

الإتاحة: https://doi.org/10.1016/j.cpcardiol.2023.102230Test
https://api.elsevier.com/content/article/PII:S0146280623006473?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0146280623006473?httpAccept=text/plainTest

المؤلفون: Avetisyan, Gayane

المصدر: Online.

مصطلحات موضوعية: Stem cell transplantation. Antibody formation. Immunity, cellular Influenza, human. Cytomegalovirus. Cytomegaolovirus infections. Influenza vaccines. Transplantation, homologous. T-lymphocytes

الوصف: Diss. (sammanfattning) Stockholm : Karolinska institutet, 2007.
Härtill 4 uppsatser.

الإتاحة: http://diss.kib.ki.se/2007/978-91-7357-339-9Test/

المؤلفون: Vicente Gil, José, Miralles, Alberto, de las Heras, Sandra, Such, Esperanza, Avetisyan, Gayane, Díaz-González, Álvaro, Santiago, Marta, Fuentes, Carolina, María Fernández, José, Lloret, Pilar, Navarro, Irene, Montesinos, Pau, Llop, Marta, Barragán, Eva

المصدر: Frontiers in Molecular Biosciences; 2024, p1-9, 9p

المؤلفون: Liquori, Alessandro, Lesende, Iván, Palomo Sanchís, Laura, Avetisyan, Gayane, Ibáñez, Mariam, González-Romero, Elisa, Boluda-Navarro, Mireia, Morote-Faubel, Mireya, Garcia-Ruiz, Cristian, Martinez-Valiente, Cristina, Santiago-Balsera, Marta, Gomez-Seguí, Inés, Sanjuan-Pla, Alejandra, Sanz, Miguel A., Sanz, Guillermo, Sole, F., Such, Esperanza, Cervera, José, Universitat Autònoma de Barcelona

مصطلحات موضوعية: Myelodysplastic syndromes, Cytogenetics, Next-generation sequencing, Myeloid neoplasm, SNP array, Karyotype

الوصف: Chromosomal abnormalities and somatic mutations are found in patients with myelodysplastic syndromes (MDS) and myelodysplastic/myeloproliferative neoplasms (MDS/MPN) in around 50-80% of cases. The identification of these alterations is important for the accurate diagnosis and prognostic classification of these patients. Often, an apparently normal or failed karyotype might lead to an inadequate estimation of the prognostic risk, and several strategies should be combined to solve these cases. The aim of this study was to introduce a novel next-generation sequencing (NGS)-based strategy for the simultaneous detection of all the clinically relevant genetic alterations associated with these disorders. We validated this approach on a large cohort of patients by comparing our findings with those obtained with standard-of-care methods (i.e., karyotype and SNP-arrays). We show that our platform represents a significant improvement on current strategies in defining diagnosis and risk stratification of patients with MDS and myeloid-related disorders. Myelodysplastic syndromes (MDS) and myelodysplastic/myeloproliferative neoplasms are clonal disorders that share most of their cytogenetic and molecular alterations. Despite the increased knowledge of the prognostic importance of genetics in these malignancies, next-generation sequencing (NGS) has not been incorporated into clinical practice in a validated manner, and the conventional karyotype remains mandatory in the evaluation of suspected cases. However, non-informative cytogenetics might lead to an inadequate estimation of the prognostic risk. Here, we present a novel targeted NGS-based assay for the simultaneous detection of all the clinically relevant genetic alterations associated with these disorders. We validated this platform in a large cohort of patients by performing a one-to-one comparison with the lesions from karyotype and single-nucleotide polymorphism (SNP) arrays. Our strategy demonstrated an approximately 97% concordance with standard clinical assays, ...

وصف الملف: application/pdf

العلاقة: Ministerio de Economía y Competitividad PI16/01113; Ministerio de Economía y Competitividad PI16/00665; Instituto de Salud Carlos III PI17/0575; Instituto de Salud Carlos III PI18/1472; Instituto de Salud Carlos III PI19/00812; Ministerio de Educación, Cultura y Deporte GV/2019/084; Agència de Gestió d'Ajuts Universitaris i de Recerca 2017SGR288; Cancers; Vol. 13 (april 2021); https://ddd.uab.cat/record/255507Test; urn:10.3390/cancers13081947; urn:oai:ddd.uab.cat:255507; urn:pmcid:PMC8072643; urn:pmc-uid:8072643; urn:oai:pubmedcentral.nih.gov:8072643; urn:pmid:33919541

الإتاحة: https://ddd.uab.cat/record/255507Test

المؤلفون: Díaz-González, Álvaro, Mora, Elvira, Avetisyan, Gayane, Furió, Santiago, De la Puerta, Rosalía, Gil, José Vicente, Liquori, Alessandro, Villamón, Eva, García-Hernández, Carmen, Santiago, Marta, García-Ruiz, Cristian, Llop, Marta, Ferrer-Lores, Blanca, Barragán, Eva, García-Palomares, Silvia, Mayordomo, Empar, Luna, Irene, Vicente, Ana, Cordón, Lourdes, Senent, Leonor

المصدر: Cancers; Jun2023, Vol. 15 Issue 11, p3039, 16p

مصطلحات موضوعية: MYELOFIBROSIS, KARYOTYPES, RISK assessment, CHROMOSOME abnormalities, RESEARCH funding, CYTOGENETICS, GENE mapping, DISEASE management, DISEASE risk factors

مستخلص: Simple Summary: Cytogenetic risk categorization is essential for the application of prognostic scores and the management of patients with myelofibrosis (MF). However, the low resolution of conventional karyotyping and the absence of metaphases are major limitations in MF. Herein, cytogenetic characterization via optical genome mapping (OGM) was performed in a cohort of 21 MF patients. To evaluate OGM impact on prognosis assessment, risk stratification scores were recalculated, including all the cytogenetic alterations uncovered. OGM satisfactorily characterized all patients with a previously unsuccessful karyotype. Additionally, the cryptic alterations detected via OGM led to an upgrade in the risk category for three patients. In conclusion, OGM may be a promising technique for cytogenetic assessment in MF. Cytogenetic assessment in myelofibrosis is essential for risk stratification and patient management. However, an informative karyotype is unavailable in a significant proportion of patients. Optical genome mapping (OGM) is a promising technique that allows for a high-resolution assessment of chromosomal aberrations (structural variants, copy number variants, and loss of heterozygosity) in a single workflow. In this study, peripheral blood samples from a series of 21 myelofibrosis patients were analyzed via OGM. We assessed the clinical impact of the application of OGM for disease risk stratification using the DIPSS-plus, GIPSS, and MIPSS70+v2 prognostic scores compared with the standard-of-care approach. OGM, in combination with NGS, allowed for risk classification in all cases, compared to only 52% when conventional techniques were used. Cases with unsuccessful karyotypes (n = 10) using conventional techniques were fully characterized using OGM. In total, 19 additional cryptic aberrations were identified in 9 out of 21 patients (43%). No alterations were found via OGM in 4/21 patients with previously normal karyotypes. OGM upgraded the risk category for three patients with available karyotypes. This is the first study using OGM in myelofibrosis. Our data support that OGM is a valuable tool that can greatly contribute to improve disease risk stratification in myelofibrosis patients. [ABSTRACT FROM AUTHOR]

: Copyright of Cancers is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Gil, José Vicente, Such, Esperanza, Sargas, Claudia, Simarro, Javier, Miralles, Alberto, Pérez, Gema, de Juan, Inmaculada, Palanca, Sarai, Avetisyan, Gayane, Santiago, Marta, Fuentes, Carolina, Fernández, José María, Vicente, Ana Isabel, Romero, Samuel, Llop, Marta, Barragán, Eva

المصدر: International Journal of Molecular Sciences; Mar2023, Vol. 24 Issue 5, p4440, 15p

مصطلحات موضوعية: LYMPHOBLASTIC leukemia, ACUTE leukemia, CUSTOM design, CHILD patients, GENE expression, DNA copy number variations

مستخلص: The molecular landscape of acute lymphoblastic leukemia (ALL) is highly heterogeneous, and genetic lesions are clinically relevant for diagnosis, risk stratification, and treatment guidance. Next-generation sequencing (NGS) has become an essential tool for clinical laboratories, where disease-targeted panels are able to capture the most relevant alterations in a cost-effective and fast way. However, comprehensive ALL panels assessing all relevant alterations are scarce. Here, we design and validate an NGS panel including single-nucleotide variants (SNVs), insertion–deletions (indels), copy number variations (CNVs), fusions, and gene expression (ALLseq). ALLseq sequencing metrics were acceptable for clinical use and showed 100% sensitivity and specificity for virtually all types of alterations. The limit of detection was established at a 2% variant allele frequency for SNVs and indels, and at a 0.5 copy number ratio for CNVs. Overall, ALLseq is able to provide clinically relevant information to more than 83% of pediatric patients, making it an attractive tool for the molecular characterization of ALL in clinical settings. [ABSTRACT FROM AUTHOR]

: Copyright of International Journal of Molecular Sciences is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)