المؤلفون: Gabriel Costa Andrade, Luciana Harumi Fujise, Atílio Maximino Fernandes, Reinaldo Azoubel

المصدر: International Archives of Otorhinolaryngology, Vol 19, Iss 03, Pp 196-199 (2015)

مصطلحات موضوعية: nasal polyps, papilloma, inverted, nasal cavity, otorhinolaryngologic neoplasms, Medicine, Otorhinolaryngology, RF1-547

الوصف: Abstract Introduction Nasal obstruction is one of the main rhinologic complaints, and two diseases must be investigated as differential diagnosis: rhinosinusal polyposis and inverted papilloma. Using traditional methods, the differential diagnosis between these diseases may be difficult. The morphometric study may be a useful tool for differential diagnosis and to define prognosis. Objective Calculate the morphometric values of rhinosinusal polyposis and inverted papilloma and compare the average of variables obtained between the groups. Methods The nasal mucus of 10 patients who had surgery in the Department of Otorhinolaryngology and Surgery of Head and Neck was studied; 5 had rhinosinusal polyposis and 5 had inverted papilloma. After the capture and print of corresponding data of each slide, the largest and smallest diameters of the nuclei were measured and the morphometric variables were calculated: average diameter, perimeter, ratio between largest and smallest diameter, volume, area, ratio of volume to area, form coefficient, contour index, and eccentricity. Results We found a significant difference (p

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1809-9777Test; https://doaj.org/toc/1809-4864Test

الوصول الحر: https://doaj.org/article/41c59caac22645f2a57f90917f3b5c87Test

المؤلفون: Wilma T. Anselmo-Lima, Eulália Sakano, Edwin Tamashiro, André Alencar Araripe Nunes, Atílio Maximino Fernandes, Elizabeth Araújo Pereira, Érica Ortiz, Fábio de Rezende Pinna, Fabrizio Ricci Romano, Francini Grecco de Melo Padua, João Ferreira Mello Junior, João Teles Junior, José Eduardo Lutaif Dolci, Leonardo Lopes Balsalobre Filho, Eduardo Macoto Kosugi, Marcelo Hamilton Sampaio, Márcio Nakanishi, Marco César Jorge dos Santos, Nilvano Alves de Andrade, Olavo de Godoy Mion, Otávio Bejzman Piltcher, Reginaldo Raimundo Fujita, Renato Roithmann, Richard Louis Voegels, Roberto Eustaquio Santos Guimarães, Roberto Campos Meireles, Victor Nakajima, Fabiana Cardoso Pereira Valera, Shirley Shizue Nagata Pignatari

المصدر: Brazilian Journal of Otorhinolaryngology, Vol 81, Iss 1, Pp 8-18 (2015)

مصطلحات موضوعية: Otorhinolaryngology, RF1-547

وصف الملف: electronic resource

العلاقة: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1808-86942015000100008&lng=en&tlng=enTest; https://doaj.org/toc/1808-8686Test

الوصول الحر: https://doaj.org/article/0deb85dfa77e4d0aa94a4cac4a4b35a9Test

المؤلفون: Atílio Maximino Fernandes, Erica Babeto, Paula Rahal, Paola Jocelan Scarin Provazzi, Claudia Augusta Hidalgo, Wilma T Anselmo-Lima

المصدر: Brazilian Journal of Otorhinolaryngology, Vol 76, Iss 2, Pp 213-218 (2010)

مصطلحات موضوعية: anexina a1, galectina 1, glucocorticoides, pólipos nasais, annexin a1, galectin 1, glucocorticoids, nasal polyps, Otorhinolaryngology, RF1-547

الوصف: A fisiopatologia da polipose rinossinusal não é totalmente compreendida, apesar de várias hipóteses em relação ao seu processo inflamatório. OBJETIVOS: Estudo prospectivo da expressão dos genes das proteínas, anexina-1 e a galectina-1, que têm ação anti-inflamatória, e sua modulação pelo glicocorticoide. MATERIAL E MÉTODOS: Onze pacientes portadores de polipose rinossinusal tiveram biopsiados seus pólipos em dois momentos: na ausência de glicocorticoide sistêmico, e na sua presença. Nas duas amostras, foi avaliada a expressão desses genes e comparada com a expressão na mucosa nasal normal do meato médio. RESULTADOS: Verificou-se que a média de expressão dos genes que codifica a anexina-1 e galectina-1 estava predominantemente aumentada, independente do uso do glicocorticoide em relação à mucosa nasal controle. Entretanto, nos pólipos sem uso de corticoide, a média de expressão do gene da anexina-1 foi significativamente maior do que nos pólipos que estavam sob uso de glicocorticoide. Com relação à galectina-1 não houve diferença significativa entre as médias de expressão antes e após o uso de glicocorticoide sistêmico. CONCLUSÃO: Os genes apresentaram um aumento da expressão na mucosa nasal polipoide, independente do uso do glicocorticoide, porém a relação destes dois genes das proteínas anti-inflamatórias com o glicocorticoide não ocorreu da mesma maneira.Rhinosinusal polyps physiopathology is not fully understand, despite numerous hypotheses regarding its inflammatory process. AIMS: a prospective study regarding the gene expression of proteins: anexin-1 and galectin-1, which has an anti-inflammatory action and is modulated by steroids. MATERIALS AND METHODS: eleven patients with rhinosinusal polyps suffered a biopsy of their polyps at two moments: in the absence of systemic steroids and during its use. In the two samples we assessed the expression of these genes and compared it to the normal nasal mucosa in the middle meatus. RESULTS: We noticed that the mean expression of the genes which code anexin-1 and galectin-1 was predominantly increased, regardless of the use of steroids in relation to the control nasal mucosa. Notwithstanding, in polyps without the use of steroids, the mean gene expression of anexin-1 was significantly higher than in the polyps which were under the use of steroids. Regarding galectin-1, there was no significant difference between the expression mean values before and after the use of systemic steroids. CONCLUSION: The genes present an expression increase in the polyp mucosa, regardless of the use of steroids; nonetheless, the relationship of these two genes of anti-inflammatory proteins with steroids did not happen the same way.

وصف الملف: electronic resource

العلاقة: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1808-86942010000200011Test; https://doaj.org/toc/1808-8694Test; https://doaj.org/toc/1808-8686Test

الوصول الحر: https://doaj.org/article/65d00c36147b475980f2e8a3187ab0eaTest

المؤلفون: Milena Moreira Arruda, Daniela Yasbek Monteiro, Atilio Maximino Fernandes, Vanessa Menegatti, Emerson Thomazzi, Ricardo Arthur Hubner, Luiz Guilherme Cernaglia Aureliano de Lima

المصدر: International Archives of Otorhinolaryngology, Vol 18, Iss 04, Pp 409-411 (2014)

مصطلحات موضوعية: clinical diagnosis, differential diagnosis, leiomyoma, inverted papilloma, nasal cavity, nasal obstruction, vascular leiomyoma, squamous papilloma, diagnosis, Medicine, Otorhinolaryngology, RF1-547

الوصف: Introduction Vascular leiomyoma of the nasal cavity is an extremely rare tumor that represents less than 1% of all vascular leiomyomas. It is more prevalent in women between the fourth and sixth decades, reaching primarily the inferior nasal turbinates. Objectives Reporting and assisting the systematization of more accurate diagnostic methods in clinical and complementary investigation of vascular leiomyoma in the nasal cavity. Resumed Report We present the case of a 49-year-old woman diagnosed with vascular leiomyoma in the nasal cavity, which manifested mainly with nasal obstruction. During investigation, computer tomography was not diagnostic, the cytologic study was not conclusive, and according to the biopsy, it was a squamous papilloma. Conclusion We suggest that the technical difficulty in obtaining an adequate amount of material for preoperative biopsy, associated with the topography of the lesion in the vestibular nasal region, may have contributed to changing the postoperative diagnosis. Thus, pathologic study of the surgical fragment is the more accurate method for diagnosis.

العلاقة: https://doaj.org/toc/1809-9777Test; https://doaj.org/toc/1809-4864Test; https://doaj.org/article/4cb0797b0c85449da9962c0b7048ba79Test

الإتاحة: https://doi.org/10.1055/s-0033-1364173Test
https://doaj.org/article/4cb0797b0c85449da9962c0b7048ba79Test

المؤلفون: Thiago Bittencourt Ottoni de Carvalho, Atílio Maximino Fernandes, Raphael Angelo Sanches

المصدر: International Archives of Otorhinolaryngology, Vol 16, Iss 1, Pp 126-129 (2012)

مصطلحات موضوعية: medicine.medical_specialty, medicine.medical_treatment, abscess, lcsh:Medicine, Palpation, tongue diseases, Swallowing, tongue, Tongue, medicine, Abscess, Dental implant, dental implant, medicine.diagnostic_test, business.industry, lcsh:R, General Medicine, lcsh:Otorhinolaryngology, medicine.disease, lcsh:RF1-547, Surgery, medicine.anatomical_structure, Etiology, Differential diagnosis, Airway, business

الوصف: Introduction: The tongue abscesses are rare even being frequently in touch with trauma, bites, and foreign bodies. It occurs because of the immunological features of the saliva and some histological and anatomical characteristics of the tongue. This work has as objective report a case of tongue abscess occurred in our job, and do a literature review. Case Report: Patient of 76 years, male, assisted with complaint of pain when swallowing with progressive evolution for a week, with a significant deterioration over three days. Report history of the surgery, for dental implant in the beginning of the period. In the admission during the oral cavity examination was evident bulging of nearly 2 × 2 cm in the middle third posterior of the right tongue, painful to palpation. Was opted the hospitalization of the patient. Was required a MRI that showed suggestive lesions of abscess in the base of the tongue in the right side. Was submitted to a clinical treatment with antibiotic therapy and surgical drainage. The patient recovered uneventfully. Discussion: The clinical manifestation from the tongue abscess can be varied. The diagnosis must be done by clinic history, correlating risk factors associated, physical exam and the confirmatory imaging exams. The etiology and the differential diagnosis must be considered depending of the place of the clinical manifestation. The approach must include the permeability of airway, clinical support, and systemic antibiotic therapy and abscess drainage. Final Considerations: The abscess of the tongue must go in the differential diagnosis of bulging of the tongue being conducted with the antibiotic therapy and surgical drainage in a daily basis.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e6ada292210ce010a6a38c919bee679aTest
https://doi.org/10.7162/s1809-48722012000100019Test

المؤلفون: Gabriel Costa de Andrade, Luciana Harumi Fujise, Atílio Maximino Fernandes, Reinaldo Azoubel

المصدر: International Archives of Otorhinolaryngology v.19 n.3 2015
International Archives of Otorhinolaryngology
Fundação Otorrinolaringologia (FORL)
instacron:FORL
International Archives of Otorhinolaryngology, Volume: 19, Issue: 3, Pages: 196-199, Published: 2015
International Archives of Otorhinolaryngology, Vol 19, Iss 03, Pp 196-199 (2015)

مصطلحات موضوعية: Nasal cavity, Pathology, medicine.medical_specialty, lcsh:Medicine, Inverted papilloma, Article, Perimeter, Nasal mucus, otorhinolaryngologic neoplasms, medicine, In patient, nasal polyps, business.industry, inverted, lcsh:R, Significant difference, Anatomy, nasal cavity, lcsh:Otorhinolaryngology, medicine.disease, lcsh:RF1-547, papilloma, medicine.anatomical_structure, Otorhinolaryngology, Papilloma, Differential diagnosis, business

الوصف: IntroductionNasal obstruction is one of the main rhinologic complaints, and two diseases must be investigated as differential diagnosis: rhinosinusal polyposis and inverted papilloma. Using traditionalmethods, the differential diagnosis between these diseases may be difficult. The morphometric study may be a useful tool for differential diagnosis and to define prognosis.Objective Calculate the morphometric values of rhinosinusal polyposis and inverted papilloma and compare the average of variables obtained between the groups.Methods The nasal mucus of 10 patients who had surgery in the Department of Otorhinolaryngology and Surgery of Head and Neck was studied; 5 had rhinosinusal polyposis and 5 had inverted papilloma. After the capture and print of corresponding data of each slide, the largest and smallest diameters of the nuclei were measured and the morphometric variables were calculated: average diameter, perimeter, ratio between largest and smallest diameter, volume, area, ratio of volume to area, form coefficient, contour index, and eccentricity.Results We found a significant difference (p < 0.05) between the two groups in the following morphometric variables: largest diameter, smallest diameter, average diameter, volume, area, perimeter, and ratio of volume to area, indicating that these parameters can be useful in diagnostic differentiation between these diseases.Conclusion We founded morphometric variables higher in patients with inverted papilloma, which can be related to the neoplastic origin of the inverted papilloma. The analysis of nuclear parameters is an instrument of great value in the differential diagnosis between rhinosinusal polyposis and inverted papilloma.

وصف الملف: text/html

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aebd306d33b49acd14b0d4729e1801a1Test
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1809-48642015000300196Test

المؤلفون: Atílio Maximino Fernandes, Fabiana Cardoso Pereira Valera, Wilma T. Anselmo-Lima

المصدر: Revista Brasileira de Otorrinolaringologia, Vol 74, Iss 2, Pp 279-283 (2008)

مصطلحات موضوعية: mecanismos fisiopatológicos, glucocorticoids, pathophysiological mechanisms, lcsh:R, citocinas, nasal polyposis, glicocorticóide, lcsh:Medicine, lcsh:Otorhinolaryngology, lcsh:RF1-547, cytokines, polipose rinossinusal

الوصف: Os glicocorticóides (GC) são drogas de escolha no tratamento clínico da polipose nasossinusal conforme recomendação da literatura. Entretanto, seus mecanismos de ação nas regressões dos sintomas clínicos e dos pólipos não são totalmente compreendidos. Sabe-se que a administração tópica e ou sistêmica dos glicocorticóides leva a variações na expressão de citocinas, quimiocinas e linfocinas, além das alterações celulares. Assim, os GC suprimem a expressão de citocinas pró-inflamatórias, de quimiocinas, de moléculas de adesão, além de estimular a transcrição de citocinas antiinflamatórias. Citocinas pró-fibróticas como a IL-11, fator básico de crescimento do fibroblasto (b-FGF) e fator de crescimento endotelial vascular (VEGF), relacionados com o crescimento do pólipo, também são suprimidos pela ação do GC. Tal ação depende fundamentalmente da interação com os seus receptores (GR), pois alguns indivíduos apresentam algum grau de resistência celular ao seu efeito, que parece estar relacionada com a presença da isoforma b do GR. Genes envolvidos nas fases de produção de imunoglobulinas, apresentação e processamento do antígeno também sofrem ação dos GC de forma variada. OBJETIVOS: Fazer uma revisão da literatura sobre os mecanismos de ação do GC na PNS. CONCLUSÃO: A compreensão desses mecanismos implicará no desenvolvimento de drogas mais eficazes na sua terapêutica.Glucocorticoids (GC) are the drugs of choice for the clinical treatment of nasal polyposis, according to the medical literature. Its mechanism of action in the regression of clinical symptoms and polyps, however, is not fully understood. The topical and/or systemic use of glucocorticoids lead to variable expression of cytokines, chemokines and lymphokines, as well as changes in cells. It is known that GC suppresses the expression of pro-inflammatory cytokines, chemokines and adhesion molecules such as ICAM-1 and E-selectin; GC also stimulate the transcription of anti-inflammatory cytokines such as TGF-b. GC suppress pro-fibrotic cytokines related to polyp growth, such as IL-11, the basic fibroblast growth factor (b-FGF), and the vascular endotelial growth factor (VEGF). The action of GC depends fundamentally on their interaction with receptors (GR); certain subjects have a degree of resistance to its effect, which appears to be related with the presence of a b isoform of GR. GC also act variably on the genes involved in immunoglobulin production, presentation, and antigen processing. AIM: We present a review of the literature on the mechanisms of GC action in nasal polyosis. CONCLUSION: Understanding the mechanism of action of GC in nasal polyposis will aid in the development of new, more efficient, drugs.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doajarticles::343309d9664ae0dff371adc16bce1b6dTest
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-72992008000200020Test

المؤلفون: Wilma T, Anselmo-Lima, Eulália, Sakano, Edwin, Tamashiro, André Alencar Araripe, Nunes, Atílio Maximino, Fernandes, Elizabeth Araújo, Pereira, Érica, Ortiz, Fábio de Rezende, Pinna, Fabrizio Ricci, Romano, Francini Grecco de Melo, Padua, João Ferreira, Mello Junior, João, Teles Junior, José Eduardo Lutaif, Dolci, Leonardo Lopes, Balsalobre Filho, Eduardo Macoto, Kosugi, Marcelo Hamilton, Sampaio, Márcio, Nakanishi, Marco César Jorge dos, Santos, Nilvano Alves de, Andrade, Olavo de Godoy, Mion, Otávio Bejzman, Piltcher, Reginaldo Raimundo, Fujita, Renato, Roithmann, Richard Louis, Voegels, Roberto Eustaquio Santos, Guimarães, Roberto Campos, Meirelles, Rodrigo, Paula Santos, Victor, Nakajima, Fabiana Cardoso Pereira, Valera, Shirley Shizue Nagata, Pignatari

المصدر: Brazilian journal of otorhinolaryngology. 81(1 Suppl 1)

مصطلحات موضوعية: Evidence-Based Medicine, Adrenal Cortex Hormones, Risk Factors, Acute Disease, Chronic Disease, Humans, Endoscopy, Sinusitis, Anti-Bacterial Agents, Rhinitis

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::7869ba8ea4cff215454c191457390786Test
https://pubmed.ncbi.nlm.nih.gov/25697512Test

المؤلفون: Janes Lopes Bonilha, Paula Rahal, Paola Jocelan Scarin Provazzi, Erica Babeto, Marilia Freitas Calmon, Atílio Maximino Fernandes, José Antônio Cordeiro, Jucimara Colombo

المساهمون: Universidade Estadual Paulista (Unesp), Faculdade de Medicina de São José do Rio Preto (FAMERP)

المصدر: Web of Science
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP

مصطلحات موضوعية: Cancer Research, NOVA1, Population, Mucous membrane of nose, Biology, Biochemistry, Exon, Gene expression, Genetics, medicine, SOLH, Nasal polyps, education, Molecular Biology, Gene, education.field_of_study, nasal polyps, methylated CpG island amplification, ATP2A1, medicine.disease, Molecular biology, Real-time polymerase chain reaction, Oncology, CpG site, PLCD3, Cancer research, Molecular Medicine, TGF beta 1, Real-time PCR

الوصف: Made available in DSpace on 2013-08-12T18:23:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-05-01 Made available in DSpace on 2013-09-30T18:41:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-05-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T14:00:54Z No. of bitstreams: 0 Made available in DSpace on 2014-05-20T14:00:54Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-05-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Nasal polyposis (NP) is a chronic inflammatory disease of the nasal mucosa characterized by the infiltration of inflammatory cells, mainly eosinophils. Although nasal polyposis occurs in 4% of the population, its physiopathology remains unclear. The aim of this study was to identify and characterize differentially expressed genes that can be used in the prognosis: treatment and elucidation of this physiopathology. To identify novel genes differentially expressed in NP, we applied real-time quantitative PCR to 11 NP samples and to a pool of total RNA from a subset of 13 normal nasal mucosa samples from human autopsies. For selecting genes, the methylated CpG island amplification technique was used. Five differentially methylated clones (ATP2A1, NOVA1, PLCD3, SOLH and TGF beta 1) were identified. However, these genes presented methylated CpG islands between exons, i.e., not in the promoter regions of the genes. Thus, as shown by real-time PCR, the ATP2A1, SOLH, PLDC3 and TGF beta 1 genes were overexpressed in NP. The genes identified in this study are probably involved in some stage of the process of formation and development of nasal polyposis, as they were highly expressed in the nasal polyp samples. UNESP, IBILCE, Dept Biol, Lab Genom Studies, BR-15054000 Sao Jose do Rio Preto, SP, Brazil Sao Jose do Rio Preto Sch Med FAMERP, Coll Med, Dept Epidemiol & Collect Hlth, Sao Jose do Rio Preto, SP, Brazil Sao Jose do Rio Preto Sch Med FAMERP, Coll Med, Dept Pathol, Sao Jose do Rio Preto, SP, Brazil Sao Jose do Rio Preto Sch Med FAMERP, Coll Med, Dept Otorhinolaryngol Head & Neck Surg, Sao Jose do Rio Preto, SP, Brazil UNESP, IBILCE, Dept Biol, Lab Genom Studies, BR-15054000 Sao Jose do Rio Preto, SP, Brazil

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ac95192e0521c95b5e33f19b1b02db8Test
https://doi.org/10.3892/mmr_00000285Test

المؤلفون: Paula Rahal, Paola Jocelan Scarin Provazzi, Claudia Augusta Hidalgo, Wilma Terezinha Anselmo-Lima, Atílio Maximino Fernandes, Erica Babeto

المساهمون: Faculdade de Medicina de São José do Rio Preto (FAMERP), Universidade Estadual Paulista (Unesp), Universidade Paulista (UNIP), Universidade de São Paulo (USP)

المصدر: Brazilian Journal of Otorhinolaryngology v.76 n.2 2010
Brazilian Journal of Otorhinolaryngology
Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial (ABORL-CCF)
instacron:ABORL-CCF
SciELO
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP

مصطلحات موضوعية: Galectina-1, Pharmacology, glucocorticoides, Glucocorticoid, nasal polyps, Galectin-1, pólipos nasais, galectina 1, Biology, anexina a1, Molecular biology, Annexin A1

الوصف: Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:44:20Z No. of bitstreams: 1 S1808-86942010000200011.pdf: 285310 bytes, checksum: 08cd1ccf649805dc8014c6faa11aeefd (MD5) Made available in DSpace on 2013-08-22T18:44:20Z (GMT). No. of bitstreams: 1 S1808-86942010000200011.pdf: 285310 bytes, checksum: 08cd1ccf649805dc8014c6faa11aeefd (MD5) Previous issue date: 2010-04-01 Made available in DSpace on 2013-09-30T19:32:24Z (GMT). No. of bitstreams: 2 S1808-86942010000200011.pdf: 285310 bytes, checksum: 08cd1ccf649805dc8014c6faa11aeefd (MD5) S1808-86942010000200011.pdf.txt: 25924 bytes, checksum: 9d80b0b3b244cfa0e900418763dce8cc (MD5) Previous issue date: 2010-04-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T14:00:45Z No. of bitstreams: 2 S1808-86942010000200011.pdf: 285310 bytes, checksum: 08cd1ccf649805dc8014c6faa11aeefd (MD5) S1808-86942010000200011.pdf.txt: 25924 bytes, checksum: 9d80b0b3b244cfa0e900418763dce8cc (MD5) Made available in DSpace on 2014-05-20T14:00:45Z (GMT). No. of bitstreams: 2 S1808-86942010000200011.pdf: 285310 bytes, checksum: 08cd1ccf649805dc8014c6faa11aeefd (MD5) S1808-86942010000200011.pdf.txt: 25924 bytes, checksum: 9d80b0b3b244cfa0e900418763dce8cc (MD5) Previous issue date: 2010-04-01 A fisiopatologia da polipose rinossinusal não é totalmente compreendida, apesar de várias hipóteses em relação ao seu processo inflamatório. OBJETIVOS: Estudo prospectivo da expressão dos genes das proteínas, anexina-1 e a galectina-1, que têm ação anti-inflamatória, e sua modulação pelo glicocorticoide. MATERIAL E MÉTODOS: Onze pacientes portadores de polipose rinossinusal tiveram biopsiados seus pólipos em dois momentos: na ausência de glicocorticoide sistêmico, e na sua presença. Nas duas amostras, foi avaliada a expressão desses genes e comparada com a expressão na mucosa nasal normal do meato médio. RESULTADOS: Verificou-se que a média de expressão dos genes que codifica a anexina-1 e galectina-1 estava predominantemente aumentada, independente do uso do glicocorticoide em relação à mucosa nasal controle. Entretanto, nos pólipos sem uso de corticoide, a média de expressão do gene da anexina-1 foi significativamente maior do que nos pólipos que estavam sob uso de glicocorticoide. Com relação à galectina-1 não houve diferença significativa entre as médias de expressão antes e após o uso de glicocorticoide sistêmico. CONCLUSÃO: Os genes apresentaram um aumento da expressão na mucosa nasal polipoide, independente do uso do glicocorticoide, porém a relação destes dois genes das proteínas anti-inflamatórias com o glicocorticoide não ocorreu da mesma maneira. Rhinosinusal polyps physiopathology is not fully understand, despite numerous hypotheses regarding its inflammatory process. AIMS: a prospective study regarding the gene expression of proteins: anexin-1 and galectin-1, which has an anti-inflammatory action and is modulated by steroids. MATERIALS and METHODS: eleven patients with rhinosinusal polyps suffered a biopsy of their polyps at two moments: in the absence of systemic steroids and during its use. In the two samples we assessed the expression of these genes and compared it to the normal nasal mucosa in the middle meatus. RESULTS: We noticed that the mean expression of the genes which code anexin-1 and galectin-1 was predominantly increased, regardless of the use of steroids in relation to the control nasal mucosa. Notwithstanding, in polyps without the use of steroids, the mean gene expression of anexin-1 was significantly higher than in the polyps which were under the use of steroids. Regarding galectin-1, there was no significant difference between the expression mean values before and after the use of systemic steroids. CONCLUSION: The genes present an expression increase in the polyp mucosa, regardless of the use of steroids; nonetheless, the relationship of these two genes of anti-inflammatory proteins with steroids did not happen the same way. Famerp Depto. Otorrinolaringologia e Cirurgia de Cabeça e Pescoço UNESP Ibilce Depto. de Biologia UNIP USP Faculdade de Medicina de Ribeirão Preto Depto. de Oftalmologia, Otorrinolaringologia e Cirurgia de Cabeça e Pescoço UNESP Ibilce Depto. de Biologia

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الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29d6a7c55d6d85fdfc99bf69606eccaaTest
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-86942010000200011Test