المؤلفون: Olcay, I. Orçun, Akçay, Berkay, Bahçeci, Mustafa, Arıcı, Aydın, Boynukalın, Kübra, Yakıcıer, Cengiz, Özpınar, Aysel, Başar, Murat

المساهمون: Tıp Fakültesi, orcid:0000-0003-0585-1077, orcid:0000-0002-7399-4929

مصطلحات موضوعية: Aneuploidy, Amino Acid Turnover, Embryo Culture Media

الوصف: Assisted reproduction technology has two significant problems: low success rates and multiple pregnancies. Because of these problems, the priority in IVF clinics is to develop a potential diagnostic test that can be used to select the embryos with the ultimate developmental competence. Aneuploidy screening as embryo selection criteria will ensure that the transferred embryos are euploid and high implantation rate. We hypothesize that aneuploidy in human preimplantation embryos could be discriminated by their amino acid metabolism profile in the spent culture media. Preimplantation genetic testing for aneuploidy results and spent embryo culture medium amino acid content were analyzed for 58 couples. The nextgeneration sequencing technique was used and coupled with TE biopsy. Forty euploid and 71 aneuploid blastocysts were evaluated. Embryos were cultured individually until day 5 or 6 of embryo development. Spent culture medium was collected after finishing the culture. There was no statistical difference between D3 and D5 embryo morphology between euploid and aneuploid embryos (p > .05). Eight amino acids, including SER, GLY, HIS, ARG, THR, ALA, PRO, and TYR, were detected in the culture medium from the blank control group, euploid group, and aneuploid group. Only TYR amino acid concentration was found significantly higher in the aneuploid group compared to the euploid group (p < .003). Tyrosine amino acid levels equal to and above 76.38 mmol/L could be considered aneuploid. Aneuploid embryos demonstrate altered amino acid turnover in vitro relative to euploid counterparts. A noninvasive method of amino acid profiling will be of value as a tool for routine preimplantation embryo selection among all patient groups.

وصف الملف: application/pdf

العلاقة: https://doi.org/10.1080/09513590.2022.2068520Test; Gynecological Endocrinology; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; https://hdl.handle.net/20.500.12445/2358Test; 38; 461; 466

الإتاحة: https://doi.org/20.500.12445/2358Test
https://doi.org/10.1080/09513590.2022.2068520Test
https://hdl.handle.net/20.500.12445/2358Test

المؤلفون: Wesevich, Victoria, Arici, Aydin

المصدر: Gynecological Endocrinology ; volume 37, issue 4, page 285-286 ; ISSN 0951-3590 1473-0766

الإتاحة: https://doi.org/10.1080/09513590.2021.1901879Test

المؤلفون: Basar, Murat, Seval-Celik, Yasemin, Osteen, Kevin, Duleba, Antoni, Taylor, Hugh, Lockwood, Charles, Arici, Aydin, Cakmak, Hakan

المصدر: Reproductive Sciences. 19(6)

مصطلحات موضوعية: Adolescent, Adult, Animals, Cells, Cultured, Chemokine CCL2, Disease Models, Animal, Endometriosis, Endometrium, Female, Gene Expression, Humans, Hydroxymethylglutaryl-CoA Reductase Inhibitors, Mice, Mice, Nude, Middle Aged, RNA, Messenger, Simvastatin, Transplantation, Heterologous

الوصف: Statins are potent inhibitors of the endogenous mevalonate pathway. Besides inhibiting cholesterol biosynthesis, statins may also demonstrate anti-inflammatory properties. Inflammation is implicated in the attachment and invasion of endometrial cells to the peritoneal surface and growth of ectopic endometrium by inducing proliferation and angiogenesis. In this study, the effect of statins on monocyte chemotactic protein 1 (MCP-1) expression in endometriotic implants in nude mouse model and in cultured endometriotic cells was evaluated. In mouse model, simvastatin decreased MCP-1 expression in a dose-dependent manner in endometriotic implants (P < .05). Similarly, both simvastatin and mevastatin revealed a dose-dependent inhibition of MCP-1 production in cultured endometriotic cells (P < .01). This inhibitory effect of the statins on MCP-1 production was reversed by the downstream substrates of the mevalonate pathway. Moreover, statins decreased MCP-1 messenger RNA expression in cultured endometriotic cells (P < .05). In conclusion, statins exert anti-inflammatory effect in endometriotic cells and could provide a potential treatment of endometriosis in the future.

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/49x28345Test

المؤلفون: Olcay, O, Bahçeci, Mustafa, MERİÇ, NESLİHAN, Akcay, Berkay, Ergun, Yagmur, BOYNUKALIN, FAZİLET KÜBRA, Arıcı, Aydın, BAŞAR, MURAT

العلاقة: 8a462a11-8234-4d27-9e63-d3e52facb1f7; https://avesis.ksbu.edu.tr/publication/details/8a462a11-8234-4d27-9e63-d3e52facb1f7/oaiTest

الإتاحة: https://doi.org/10.26502/acbr.50170357Test
https://avesis.ksbu.edu.tr/publication/details/8a462a11-8234-4d27-9e63-d3e52facb1f7/oaiTest

المؤلفون: Cakmak, Hakan, Seval-Celik, Yasemin, Arlier, Sefa, Guzeloglu-Kayisli, Ozlem, Schatz, Frederick, Arici, Aydin, Kayisli, Umit A.

المصدر: REPRODUCTIVE SCIENCES 25(4) 587-597

الوصف: Background: Local pro-inflammatory environment and enhanced cell survival contribute to the endometriosis development. A serine/threonine kinase p38 mitogen-activated protein kinase (MAPK) mediates intracellular signaling of cytokine production, cell proliferation, and apoptosis in different cell types. The current study compares p38 MAPK activity in normal endometrium and endometriosis, and assesses role(s) of p38 MAPK on cytokine production and cell survival in endometriosis.

العلاقة: https://aperta.ulakbim.gov.tr/record/30943Test; oai:zenodo.org:30943

الإتاحة: https://aperta.ulakbim.gov.tr/record/30943Test

المؤلفون: Arlier, Sefa, Murk, William, Guzeloglu-Kayisli, Ozlem, Semerci, Nihan, Larsen, Kellie, Tabak, Mehmet S., Arici, Aydin, Schatz, Frederick, Lockwood, Charles J., Kayisli, Umit A.

المصدر: AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY 78(6)

الوصف: Problem: The role of extracellular signal-regulated kinase (ERK) 1/2-mediated angiogenesis during endometriotic nidation is unknown. We posit that ERK1/2-induced angioblast differentiation and proliferation promotes ectopic endometrial angiogenesis.

العلاقة: https://aperta.ulakbim.gov.tr/record/45811Test; oai:zenodo.org:45811

الإتاحة: https://aperta.ulakbim.gov.tr/record/45811Test

المؤلفون: Yen, Chih-Feng, Kim, Sung Hoon, Liao, Shuen-Kuei, Atabekoglu, Cem, Uckac, Serpil, Arici, Aydin, Arlier, Sefa, Lee, Chyi-Long, Wang, Hsin-Shih, Kayisli, Umit A.

المصدر: Fertility and Sterility ; volume 107, issue 3, page 803-812 ; ISSN 0015-0282

مصطلحات موضوعية: Obstetrics and Gynecology, Reproductive Medicine

الإتاحة: https://doi.org/10.1016/j.fertnstert.2016.11.025Test
https://api.elsevier.com/content/article/PII:S001502821663025X?httpAccept=text/plainTest
https://api.elsevier.com/content/article/PII:S001502821663025X?httpAccept=text/xmlTest

المؤلفون: Tan, Orkun, Ornek, Turkan, Fadiel, Ahmed, Carrick, Kelley S., Arici, Aydin, Doody, Kevin, Carr, Bruce R., Naftolin, Frederick

المصدر: Fertility and Sterility ; volume 97, issue 1, page 192-199.e2 ; ISSN 0015-0282

مصطلحات موضوعية: Obstetrics and Gynecology, Reproductive Medicine

الإتاحة: https://doi.org/10.1016/j.fertnstert.2011.10.039Test
https://api.elsevier.com/content/article/PII:S0015028211027245?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0015028211027245?httpAccept=text/plainTest

المؤلفون: Guzel, Elif, Basar, Murat, Ocak, Nehir, Arici, Aydin, Kayisli, Umit A.

المصدر: BIOLOGY OF REPRODUCTION 85(1) 121-127

الوصف: The human endometrium is a dynamic tissue that undergoes cyclic changes under the influence of steroid hormones as well as numerous local paracrine and autocrine factors. Heat shock 70 kDa protein (HSPA5; also known as GRP78/BiP), a molecular chaperone within the endoplasmic reticulum, plays crucial roles in normal cellular processes as well as in stress conditions, in which it is a central regulator for the unfolded protein response (UPR). We hypothesized that HSPA5 expression level is variable throughout the menstrual cycle in human endometrium and that estrogen signaling cross-talks with UPR signaling by interacting with HSPA5. HSPA5 expression throughout the menstrual cycle was evaluated in vivo in normal human endometrium. Using in vitro techniques, we then assessed the bidirectional regulation of HSPA5 and estrogen signaling in human endometrial glandular (Ishikawa) and stromal cells (ESC). HSPA5 immunoreactivity in endometrial glandular and stromal cells was cycle-dependent, and was significantly higher in phases of the menstrual cycle when estradiol (E-2) levels are known to be the lowest compared with the rest of the cycle (P < 0.001). E-2 did not affect HSPA5 expression after 8-24 h incubation in Ishikawa cells and ESC in vitro. However, tunicamycin-induced HSPA5 expression was significantly lowered in these cells when pretreated with E-2 (P, 0.01 and P < 0.05, respectively). On the other hand, tunicamycin decreased E-2 up-regulated alkaline phosphatase activity (P < 0.001). In conclusion, there is cycle-dependent HSPA5 expression with a possible inverse correlation between HSPA5 expression and E-2 levels in human endometrium. We suggest that estrogen signaling cross-talks with the UPR cascade by interacting with HSPA5, as supported by our in vitro findings.

العلاقة: https://aperta.ulakbim.gov.tr/record/20271Test; oai:zenodo.org:20271

الإتاحة: https://aperta.ulakbim.gov.tr/record/20271Test

المؤلفون: Kayisli, Umit A., Arici, Aydin, Seval, Yasemin, Cinar, Ozgur, Uz, Yesim H., Cakmak, Hakan, Ulukus, Murat

مصطلحات موضوعية: endometriosis, Akt phosphorylation, oestradiol

الوصف: WOS: 000273671000017 ; PubMed ID: 20031030 ; Protein kinase B (PKB/Akt), a serine/threonine kinase, regulates the function of many cellular proteins involved in apoptosis and proliferation. It was postulated that there is a higher Akt activity in endometriosis compared with normal endometrium, and that oestrogen may be one of the factors responsible for the high Akt activation in endometriotic cells. Phospho-Akt (pAkt) concentrations in normal, eutopic and ectopic endometrial tissues were compared by immunohistochemistry, and a higher pAkt immunoreactivity was revealed in eutopic and ectopic endometrium compared with normal endometrium, in vivo. Higher Akt phosphorylation in stromal cells from eutopic endometrium was observed, when compared with normal, ill vitro (P 0.05). Akt phosphorylation was rapidly (2-10 min) stimulated when endometrial stromal cells from normal and endometriosis patients were treated with 17 beta-oestradiol. In endometrial stromal cells from the endometriosis group, ICI 182,780 (10, a specific oestrogen receptor antagonist) failed to antagonize the effect of oestradiol when combined with oestradiol, and revealed a stimulatory effect on Akt phosphorylation when given alone (P 0.05). In Conclusion, since Akt affects cell survival, it is suggested that increased Akt phosphorylation may be related to the altered apoptosis/proliferation harmony in endometriosis, and therefore Akt may play a critical role in the pathogenesis of endometriosis.

العلاقة: Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; Reproductive Biomedicine Online; https://hdl.handle.net/11454/42658Test; https://doi.org/10.1016/j.rbmo.2009.10.001Test; 19; 864; 871

الإتاحة: https://doi.org/10.1016/j.rbmo.2009.10.001Test
https://hdl.handle.net/11454/42658Test