المؤلفون: Steel, Lynsey, Ansell, David M., Amaya, Enrique, Cartmell, Sarah H.

المصدر: Steel , L , Ansell , D M , Amaya , E & Cartmell , S H 2022 , ' Ppia and ywhaz constitute a stable pair of reference genes during electrical stimulation in mesenchymal stem cells ' , Applied Sciences (Switzerland) , vol. 12 , no. 1 , 153 . https://doi.org/10.3390/app12010153Test

مصطلحات موضوعية: Electrical stimulation, Gene expression, Housekeeping genes, Mesenchymal stem cells, Reference genes, Reverse transcription-PCR

الوصف: Mesenchymal stem cells (MSCs) are multipotent adult stem cells with great potential in regenerative medicine. One method for stimulating proliferation and differentiation of MSCs is via electrical stimulation (ES). A valuable approach for evaluating the response of MSCs to ES is to assess changes in gene expression, relative to one or more reference genes. In a survey of 25 publications that used ES on cells, 70% selected GAPDH as the reference gene. We conducted a study to assess the suitability of six potential reference genes on an immortalized human MSC line following direct current ES at seeding densities of 5000 and 10,000 cells/cm 2 . We employed three methods to validate the most stable reference genes from qRT-PCR data. Our findings show that GAPDH and ACTB exhibit reduced stability when seeded at 5000 cell/cm 2 . In contrast, we found that the most stable genes across both plating densities and stimulation regimes were PPIA and YWHAZ. Thus, in ES gene expression studies in MSCs, we support the use of PPIA and YWHAZ as an optimal reference gene pair, and discourage the use of ACTB and GAPDH at lower seeding densities. However, it is strongly recommended that similar verification studies are carried out based on cell type and different ES conditions.

وصف الملف: application/pdf

الإتاحة: https://doi.org/10.3390/app12010153Test
https://research.manchester.ac.uk/en/publications/0e89eee7-5cdf-4ce5-b4bb-9124366dacbeTest
https://pure.manchester.ac.uk/ws/files/211459235/applsci_12_00153.pdfTest

المؤلفون: Crompton, Rachel A., Williams, Helen, Campbell, Laura, Hui Kheng, Lim, Saville, Charis, Ansell, David M., Reid, Adam, Wong, Jason, Vardy, Leah A., Hardman, Matthew J., Cruickshank, Sheena M.

المساهمون: Biotechnology and Biological Sciences Research Council, University of Manchester, Wellcome Trust, Medical Research Council

المصدر: Journal of Investigative Dermatology ; volume 142, issue 4, page 1206-1216.e8 ; ISSN 0022-202X

مصطلحات موضوعية: Cell Biology, Dermatology, Molecular Biology, Biochemistry

الإتاحة: https://doi.org/10.1016/j.jid.2021.09.009Test
https://api.elsevier.com/content/article/PII:S0022202X21022880?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0022202X21022880?httpAccept=text/plainTest

المؤلفون: Nicu, Carina, O’Sullivan, James D.B., Ramos, Raul, Timperi, Ludovica, Lai, Tiffany, Farjo, Nilofer, Farjo, Bessam, Pople, Jenny, Bhogal, Ranjit, Hardman, Jonathan A., Plikus, Maksim V., Ansell, David M., Paus, Ralf

المساهمون: Engineering and Physical Sciences Research Council, Unilever, Manchester Biomedical Research Centre, Biotechnology and Biological Sciences Research Council

المصدر: Journal of Investigative Dermatology ; volume 141, issue 7, page 1633-1645.e13 ; ISSN 0022-202X

الإتاحة: https://doi.org/10.1016/j.jid.2020.12.019Test
https://api.elsevier.com/content/article/PII:S0022202X21000087?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0022202X21000087?httpAccept=text/plainTest

المؤلفون: Nicu, Carina, Pople, Jennifer, Bonsell, Laura, Bhogal, Ranjit, Ansell, David M., Paus, Ralf

المساهمون: National Institute on Handicapped Research, Biotechnology and Biological Sciences Research Council

المصدر: Experimental Dermatology ; volume 27, issue 6, page 589-602 ; ISSN 0906-6705 1600-0625

الوصف: Dermal white adipose tissue ( DWAT ) is a main component of human skin, composed of individual lipid‐laden mesenchymal cells known as dermal adipocytes ( DA s). Besides their well‐known role in lipid storage and release, DA s also promote skin immunity, wound healing and hair follicle cycling and are important players in cutaneous neuroendocrinology. The ever‐growing insights into DWAT functions, albeit mostly in mice, have invited speculation that it may be involved in multiple skin diseases ranging from fibrosis to alopecia and psoriasis, thus designating human DWAT a clinically relevant, but as yet insufficiently investigated skin compartment. Therefore, this practical, user‐friendly guide aims to introduce the techniques available to study human DWAT in situ and ex vivo, including immunohistochemistry, immunofluorescence microscopy and analysis via quantitative immunohistomorphometry. Here, we provide information on a collection of stains comprising pre‐adipocyte (Pref1) and mature adipocyte markers (Perilipin1, Caveolin1), as well as various lipid (OilRedO, BODIPY ) and histochemical stains (H&E, trichrome) available for use on human DWAT . We offer the reader guidelines on fixing, processing and staining human DA s and highlight caveats and solutions to common problems that one may encounter when studying this fascinating skin compartment. We also suggest standard methods for conducting quantitative immunohistomorphometry on human DWAT and its individual adipocytes to quantify cell size, number, lipid content and fluorescence intensity of adipose‐specific markers. Finally, we briefly introduce in situ hybridization, transmission electron microscopy and essentials of magnetic resonance imaging imaging as additional tools for instructively interrogating this largest, but still least‐known compartment of human skin.

الإتاحة: https://doi.org/10.1111/exd.13549Test

المؤلفون: Ansell, David M., Marsh, Charlotte, Walker, Liam, Hardman, Matthew J., Holden, Kirsty

المصدر: Journal of Investigative Dermatology ; volume 138, issue 4, page 994-997 ; ISSN 0022-202X

مصطلحات موضوعية: Cell Biology, Dermatology, Molecular Biology, Biochemistry

الإتاحة: https://doi.org/10.1016/j.jid.2017.10.020Test
https://api.elsevier.com/content/article/PII:S0022202X17330920?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0022202X17330920?httpAccept=text/plainTest

المؤلفون: Imanishi, Hisayoshi, Ansell, David M., Chéret, Jérémy, Harries, Matthew, Bertolini, Marta, Sepp, Norbert, Bíró, Tamás, Poblet, Enrique, Jimenez, Francisco, Hardman, Jonathan, Panicker, Sreejith Parameswara, Ward, Christopher M., Paus, Ralf

المصدر: Journal of Investigative Dermatology ; volume 138, issue 3, page 511-519 ; ISSN 0022-202X

مصطلحات موضوعية: Cell Biology, Dermatology, Molecular Biology, Biochemistry

الإتاحة: https://doi.org/10.1016/j.jid.2017.09.047Test
https://api.elsevier.com/content/article/PII:S0022202X1733083X?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0022202X1733083X?httpAccept=text/plainTest

المؤلفون: Stojadinovic, Olivera, Wikramanayake, Tongyu C., Villasante Fricke, Alexandra C., Yin, Natalie C., Liang, Liang, Hinde, Eleanor, Escandon, Julia, Tomic-Canic, Marjana, Ansell, David M., Paus, Ralf, Jimenez, Joaquin J.

المصدر: Stojadinovic , O , Wikramanayake , T C , Villasante Fricke , A C , Yin , N C , Liang , L , Hinde , E , Escandon , J , Tomic-Canic , M , Ansell , D M , Paus , R & Jimenez , J J 2017 , ' Wound healing protects against chemotherapy-induced alopecia in young rats via up-regulating interleukin-1β-mediated signaling ' , Heliyon , vol. 3 , no. 5 , e00309 . https://doi.org/10.1016/j.heliyon.2017.e00309Test

مصطلحات موضوعية: Medicine, ResearchInstitutes_Networks_Beacons/MICRA, Manchester Institute for Collaborative Research on Ageing

الوصف: Wound healing is a complex process regulated by various cell types and a plethora of mediators. While interactions between wounded skin and the hair follicles (HFs) could induce HF neogenesis or promote wound healing, it remains unknown whether the wound healing-associated signaling milieu can be manipulated to protect against alopecia, such as chemotherapy-induced alopecia (CIA). Utilizing a well-established neonatal rat model of CIA, we show here that skin wounding protects from alopecia caused by several clinically relevant chemotherapeutic regimens, and that protection is dependent on the time of wounding and hair cycle stage. Gene expression profiling unveiled a significant increase in interleukin-1 beta (IL-1β) mediated signaling by skin wounding. Subsequently, we showed that IL-1β is sufficient and indispensable for mediating the CIA-protective effect. Administration of IL-1β alone to unwounded rats exhibited local CIA protection while IL-1β neutralization abrogated CIA protection by wounding. Mechanistically, IL-1β retarded postnatal HF morphogenesis, making HFs at the wound sites or IL-1β treated areas damage-resistant while the rats developed total alopecia elsewhere. We conclude that wound healing switches the cutaneous cytokine milieu to an IL-1β-dominated state thus retarding HF growth progression and rendering the HFs resistant to chemotherapy agents. In the future, manipulation of HF progression through interfering with the IL-1β signaling milieu may provide therapeutic benefits to a variety of conditions, from prevention of CIA to inhibition of hair growth and treatment of hirsutism.

الإتاحة: https://doi.org/10.1016/j.heliyon.2017.e00309Test
https://research.manchester.ac.uk/en/publications/66be5803-f6d4-44ab-b215-62e6d3f5268bTest
http://www.scopus.com/inward/record.url?scp=85020032114&partnerID=8YFLogxKTest

المؤلفون: Ansell, David M., Marsh, Charlotte, Walker, Liam, Hardman, Matthew J., Holden, Kirsty

مصطلحات موضوعية: Cell Biology, Biochemistry, Molecular Biology, Dermatology, Health and Health Inequalities

العلاقة: https://hull-repository.worktribe.com/output/499027Test; Journal of Investigative Dermatology; Volume 138; Issue 4; Pagination 994-997; https://hull-repository.worktribe.com/file/499027/1/ArticleTest

الإتاحة: https://doi.org/10.1016/j.jid.2017.10.020Test
https://hull-repository.worktribe.com/file/499027/1/ArticleTest
https://hull-repository.worktribe.com/output/499027Test

المؤلفون: Garcin, Clare L., Ansell, David M.

المصدر: Experimental Dermatology ; volume 26, issue 2, page 101-104 ; ISSN 0906-6705 1600-0625

الوصف: The hair follicle has an established role in wound re‐epithelialisation, a phenomenon that has been appreciated since at least the first half of the last century. The bulge niche, one location of hair follicle epithelial stem cells has been of particular interest to researchers over recent years, with numerous studies showing its ability to directly contribute to epidermal repair. However, recent work has highlighted other progenitor regions of the hair follicle that appear to act as stem cells during epidermal repair. In addition, several studies within the last 12 months have questioned the importance of the bulge during re‐epithelialisation, producing conflicting literature. Here we provide a new model to demonstrate how several important differences in experimental design between studies could account for these seemingly opposing findings, which may have implications for how future studies are conducted.

الإتاحة: https://doi.org/10.1111/exd.13184Test

المؤلفون: Garcin, Clare L, Ansell, David M, Headon, Denis J, Paus, Ralf, Hardman, Matthew J

المصدر: Garcin , C L , Ansell , D M , Headon , D J , Paus , R & Hardman , M J 2016 , ' Hair Follicle Bulge Stem Cells Appear Dispensable for the Acute Phase of Wound Re-epithelialization ' , Stem Cells , vol. 34 , no. 5 , pp. 1377-1385 . https://doi.org/10.1002/stem.2289Test

الوصف: The cutaneous healing response has evolved to occur rapidly, in order to minimize infection and to re-establish epithelial homeostasis. Rapid healing is achieved through complex coordination of multiple cell types, which importantly includes specific cell populations within the hair follicle (HF). Under physiological conditions, the epithelial compartments of HF and interfollicular epidermis remain discrete, with K15(+ve) bulge stem cells contributing progeny for HF reconstruction during the hair cycle and as a basis for hair shaft production during anagen. Only upon wounding do HF cells migrate from the follicle to contribute to the neo-epidermis. However, the identity of the first-responding cells, and in particular whether this process involves a direct contribution of K15(+ve) bulge cells to the early stage of epidermal wound repair remains unclear. Here we demonstrate that epidermal injury in murine skin does not induce bulge activation during early epidermal wound repair. Specifically, bulge cells of uninjured HFs neither proliferate nor appear to migrate out of the bulge niche upon epidermal wounding. In support of these observations, Diphtheria toxin-mediated partial ablation of K15(+ve) bulge cells fails to delay wound healing. Our data suggest that bulge cells only respond to epidermal wounding during later stages of repair. We discuss that this response may have evolved as a protective safeguarding mechanism against bulge stem cell exhaust and tumorigenesis. Stem Cells 2016;34:1377-1385.

الإتاحة: https://doi.org/10.1002/stem.2289Test
https://research.manchester.ac.uk/en/publications/66303e56-b48f-47e9-91ef-92425b260ab3Test