المؤلفون: Wouter N. Leonhard, Xuewen Song, Anish A. Kanhai, Ioan-Andrei Iliuta, Andrea Bozovic, Gregory R. Steinberg, Dorien J.M. Peters, York Pei

المصدر: EBioMedicine, Vol 47, Iss , Pp 436-445 (2019)

مصطلحات موضوعية: Medicine, Medicine (General), R5-920

الوصف: Background: Multiple preclinical studies have highlighted AMP-activated protein kinase (AMPK) as a potential therapeutic target for autosomal dominant polycystic kidney disease (ADPKD). Both metformin and canagliflozin indirectly activate AMPK by inhibiting mitochondrial function, while salsalate is a direct AMPK activator. Metformin, canagliflozin and salsalate (a prodrug dimer of salicylate) are approved for clinical use with excellent safety profile. Although metformin treatment had been shown to attenuate experimental cystic kidney disease, there are concerns that therapeutic AMPK activation in human kidney might require a higher oral metformin dose than can be achieved clinically. Methods: In this study, we tested metformin-based combination therapies for their additive (metformin plus canagliflozin) and synergistic (metformin plus salsalate) effects and each drug individually in an adult-onset conditional Pkd1 knock-out mouse model (n = 20 male/group) using dosages expected to yield clinically relevant drug levels. Findings: Compared to untreated mutant mice, treatment with salsalate or metformin plus salsalate improved kidney survival (i.e. blood urea nitrogen

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S235239641930564XTest; https://doaj.org/toc/2352-3964Test

الوصول الحر: https://doaj.org/article/451a2a83c9fd47758a0b4b03c9d4dbd2Test

المؤلفون: Lindsie A. Blencowe, Andrea Božović, Evelyn Wong, Vathany Kulasingam, Angela M. Cheung

المصدر: Bone Reports, Vol 20, Iss , Pp 101737- (2024)

مصطلحات موضوعية: Pentosidine, Liquid chromatography-tandem mass spectrometry, Advanced glycation end product, Osteoporosis, Diseases of the musculoskeletal system, RC925-935

الوصف: Pentosidine (PEN) is an Advanced Glycation End-product (AGE) that is known to accumulate in bone collagen with aging and contribute to fracture risk. The PEN content in bone is correlated with serum PEN, making it an attractive, potential osteoporosis biomarker. We sought to develop a method for quantifying PEN in stored serum. After conducting a systematic narrative review of PEN quantification methodologies, we developed a liquid chromatography tandem mass spectrometry (LC-MS/MS) method for quantifying total serum PEN. Our method is both sensitive and precise (LOD 2 nM, LOQ 5 nM, %CV < 6.5 % and recovery 91.2–100.7 %). Our method is also equivalent or better than other methods identified in our review. Additionally, LC-MS/MS avoids the pitfalls and limitations of using fluorescence as a means of detection and could be adapted to investigate a broad range of AGE compounds.

العلاقة: http://www.sciencedirect.com/science/article/pii/S2352187224000044Test; https://doaj.org/toc/2352-1872Test; https://doaj.org/article/8680c46fed064839891147bc5b1c35baTest

الإتاحة: https://doi.org/10.1016/j.bonr.2024.101737Test
https://doaj.org/article/8680c46fed064839891147bc5b1c35baTest

المؤلفون: Yanhong Li, Alex Boshart, Rohan John, Stephen C. Juvet, Andrea Bozovic, S. Moshkelgosha, Emilie Chan, Sofia Farkona, Igor Jurisica, S. Joseph Kim, Sergi Clotet-Freixas, Andrzej Chruscinski, Jishnu Das, Tereza Martinu, Yun Niu, Vathany Kulasingam, Olusegun Famure, Syed Ashiqur Rahman, Julie Anh Dung Van, Ana Konvalinka, Max Kotlyar, Peixuen Chen, Chiara Pastrello, Caitriona M. McEvoy, Ihor Batruch, Madhurangi Arambewela

المصدر: J Am Soc Nephrol

مصطلحات موضوعية: Graft Rejection, Male, Proteomics, 0301 basic medicine, Pathology, Galectin 1, Biopsy, Kidney Glomerulus, Gene Expression, 030230 surgery, Basement Membrane, Extracellular matrix, 0302 clinical medicine, Laminin, Glutathione Transferase, Kidney, biology, medicine.diagnostic_test, Receptor-Like Protein Tyrosine Phosphatases, Class 3, General Medicine, Middle Aged, Allografts, Extracellular Matrix, Cysteine Endopeptidases, Kidney Tubules, medicine.anatomical_structure, Nephrology, Matrix Metalloproteinase 2, Female, Matrix Metalloproteinase 3, Tumor necrosis factor alpha, Renal biopsy, Adult, medicine.medical_specialty, Antibodies, Cell Line, Nephrin, Necrosis, 03 medical and health sciences, Clinical Research, medicine, Humans, Acute tubular necrosis, Aged, Basement membrane, urogenital system, Tumor Necrosis Factor-alpha, business.industry, Histocompatibility Antigens Class I, Membrane Proteins, medicine.disease, Cathepsins, Kidney Transplantation, 030104 developmental biology, biology.protein, business

الوصف: BACKGROUND: Antibody-mediated rejection (AMR) accounts for >50% of kidney allograft loss. Donor-specific antibodies (DSA) against HLA and non-HLA antigens in the glomeruli and the tubulointerstitium cause AMR while inflammatory cytokines such as TNFα trigger graft injury. The mechanisms governing cell-specific injury in AMR remain unclear. METHODS: Unbiased proteomic analysis of laser-captured and microdissected glomeruli and tubulointerstitium was performed on 30 for-cause kidney biopsy specimens with early AMR, acute cellular rejection (ACR), or acute tubular necrosis (ATN). RESULTS: A total of 107 of 2026 glomerular and 112 of 2399 tubulointerstitial proteins was significantly differentially expressed in AMR versus ACR; 112 of 2026 glomerular and 181 of 2399 tubulointerstitial proteins were significantly dysregulated in AMR versus ATN (P

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fcb8ae6e194245127d382f3f1925b585Test
https://doi.org/10.1681/asn.2020030286Test

المؤلفون: Emil List Larsen, Frank Geurts, Max Nieuwdorp, Marcel H.A. Muskiet, Daan J Touw, Henrik E. Poulsen, A.H. Jan Danser, Ewout J. Hoorn, Anna L. Emanuel, Daniël H. van Raalte, Erik J.M. van Bommel, Jaap A. Joles, Andrea Bozovic, Michaël J.B. van Baar, Mark M. Smits, Lennart Tonneijck, Mark H.H. Kramer

المساهمون: Pharmaceutical Analysis, Critical care, Anesthesiology, Peri-operative and Emergency medicine (CAPE), Groningen Research Institute for Asthma and COPD (GRIAC), Biopharmaceuticals, Discovery, Design and Delivery (BDDD), Nanomedicine & Drug Targeting, Medicinal Chemistry and Bioanalysis (MCB), Experimental Vascular Medicine, Vascular Medicine, ACS - Diabetes & metabolism, AGEM - Digestive immunity, AGEM - Endocrinology, metabolism and nutrition, Internal Medicine, Internal medicine, AII - Inflammatory diseases

المصدر: Kidney International, 97(1), 202-212. ELSEVIER SCIENCE INC
Kidney international, 97(1), 202-212. Nature Publishing Group
Kidney International, 97(1), 202-212. Elsevier Inc.
van Bommel, E J M, Muskiet, M H A, van Baar, M J B, Tonneijck, L, Smits, M M, Emanuel, A L, Bozovic, A, Danser, A H J, Geurts, F, Hoorn, E J, Touw, D J, Larsen, E L, Poulsen, H E, Kramer, M H H, Nieuwdorp, M, Joles, J A & van Raalte, D H 2020, ' The renal hemodynamic effects of the SGLT2 inhibitor dapagliflozin are caused by post-glomerular vasodilatation rather than pre-glomerular vasoconstriction in metformin-treated patients with type 2 diabetes in the randomized, double-blind RED trial ', Kidney International, vol. 97, no. 1, pp. 202-212 . https://doi.org/10.1016/j.kint.2019.09.013Test
Kidney International, 97(1), 202-212. Nature Publishing Group

مصطلحات موضوعية: 0301 basic medicine, Male, renal hemodynamics, 030232 urology & nephrology, Type 2 diabetes, Kidney, urologic and male genital diseases, HYPERFILTRATION, chemistry.chemical_compound, 0302 clinical medicine, Glucosides, SGLT2 inhibition, Gliclazide, Diabetic Nephropathies, Dapagliflozin, RISK, Middle Aged, ADENOSINE, Metformin, Vasodilation, medicine.anatomical_structure, Treatment Outcome, Nephrology, Female, type 2 diabetes, Glomerular hyperfiltration, medicine.drug, Glomerular Filtration Rate, medicine.medical_specialty, Urology, Renal function, MECHANISMS, 03 medical and health sciences, Double-Blind Method, SDG 3 - Good Health and Well-being, medicine, Humans, Benzhydryl Compounds, Sodium-Glucose Transporter 2 Inhibitors, Aged, Glycated Hemoglobin, business.industry, KIDNEY-DISEASE, Effective renal plasma flow, medicine.disease, diabetic kidney disease, Filtration fraction, 030104 developmental biology, chemistry, Diabetes Mellitus, Type 2, Vasoconstriction, Vascular resistance, business, RESISTANCE, RESPONSES

الوصف: Sodium-glucose cotransporter 2 inhibitors (SGLT2i) improve hard renal outcomes in type 2 diabetes. This is possibly explained by the fact that SGLT2i normalize the measured glomerular filtration rate (mGFR) by increasing renal vascular resistance, as was shown in young people with type 1 diabetes and glomerular hyperfiltration. Therefore, we compared the renal hemodynamic effects of dapagliflozin with gliclazide in type 2 diabetes. The mGFR and effective renal plasma flow were assessed using inulin and para-aminohippurate clearances in the fasted state, during clamped euglycemia (5 mmol/L) and during clamped hyperglycemia (15 mmol/L). Filtration fraction and renal vascular resistance were calculated. Additionally, factors known to modulate renal hemodynamics were measured. In 44 people with type 2 diabetes on metformin monotherapy (Hemoglobin A1c 7.4%, mGFR 113 mL/min), dapagliflozin versus gliclazide reduced mGFR by 5, 10, and 12 mL/min in the consecutive phases while both agents similarly improved Hemoglobin A1c (-0.48% vs -0.65%). Dapagliflozin also reduced filtration fraction without increasing renal vascular resistance, and increased urinary adenosine and prostaglandin concentrations. Gliclazide did not consistently alter renal hemodynamic parameters. Thus, beyond glucose control, SGLT2i reduce mGFR and filtration fraction in type 2 diabetes. The fact that renal vascular resistance was not increased by dapagliflozin suggests that this is due to post-glomerular vasodilation rather than pre-glomerular vasoconstriction.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ade058080ace5fb4db4e6592316e47faTest
https://doi.org/10.1016/j.kint.2019.09.013Test

المؤلفون: Grace E. Lilly, Frederick A. Dick, Pirunthan Perampalam, Vathany Kulasingam, Haider M. Hassan, Daniel Thompsen Passos, Joseph Torchia, Andrea Bozovic, Patti K. Kiser

المصدر: Paediatrics Publications
J Clin Invest

مصطلحات موضوعية: 0301 basic medicine, Amyloid, Apolipoprotein B, Retinoblastoma-Like Protein p107, Mice, 03 medical and health sciences, 0302 clinical medicine, Transcriptional repressor complex, medicine, Animals, Immunoglobulin Light-chain Amyloidosis, Epigenetics, E2F, Apolipoproteins A, Mice, Knockout, biology, Cell growth, Amyloidosis, General Medicine, medicine.disease, 3. Good health, Cell biology, 030104 developmental biology, Histone, Organ Specificity, Multiprotein Complexes, 030220 oncology & carcinogenesis, biology.protein, Research Article

الوصف: DREAM (Dp, Rb-like, E2F, and MuvB) is a transcriptional repressor complex that regulates cell proliferation, and its loss causes neonatal lethality in mice. To investigate DREAM function in adult mice, we used an assembly-defective p107 protein and conditional deletion of its redundant family member p130. In the absence of DREAM assembly, mice displayed shortened survival characterized by systemic amyloidosis but no evidence of excessive cellular proliferation. Amyloid deposits were found in the heart, liver, spleen, and kidneys but not the brain or bone marrow. Using laser-capture microdissection followed by mass spectrometry, we identified apolipoproteins as the most abundant components of amyloids. Intriguingly, apoA-IV was the most detected amyloidogenic protein in amyloid deposits, suggesting apoA-IV amyloidosis (AApoAIV). AApoAIV is a recently described form, whereby WT apoA-IV has been shown to predominate in amyloid plaques. We determined by ChIP that DREAM directly regulated Apoa4 and that the histone variant H2AZ was reduced from the Apoa4 gene body in DREAM’s absence, leading to overexpression. Collectively, we describe a mechanism by which epigenetic misregulation causes apolipoprotein overexpression and amyloidosis, potentially explaining the origins of nongenetic amyloid subtypes.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f17ef4c54d57d4b3cb1143c0013c0a32Test
https://ir.lib.uwo.ca/context/paedpub/article/2159/viewcontent/JCI140903.v2.pdfTest

المؤلفون: Lalit Saini, Jack T Seki, Deepali Kumar, Eshetu G Atenafu, David EC Cole, Betty YL Wong, Andrea Božović, Joseph M Brandwein

المصدر: Canadian Journal of Infectious Diseases and Medical Microbiology, Vol 25, Iss 5, Pp 271-276 (2014)

مصطلحات موضوعية: Infectious and parasitic diseases, RC109-216, Microbiology, QR1-502

الوصف: INTRODUCTION: Voriconazole plasma concentrations have been correlated with oral dosing in healthy subjects, but have been poorly characterized in ill patients with hematological malignancies receiving intensive chemotherapy.

العلاقة: http://dx.doi.org/10.1155/2014/214813Test; https://doaj.org/toc/1712-9532Test; https://doaj.org/article/6c379212e1fc49e4aa8c3acb7be885d9Test

الإتاحة: https://doi.org/10.1155/2014/214813Test
https://doaj.org/article/6c379212e1fc49e4aa8c3acb7be885d9Test

المؤلفون: Sofia Farkona, Sergi Clotet-Freixas, Yun Niu, Peixuen Chen, Yanhong Li, Madhurangi Arambewela, Emilie Chan, Rohan John, S. Moshkelgosha, Max Kotlyar, Andrzej Chruscinski, Joseph Kim, Vathany Kulasingam, Chiara Pastrello, Ana Konvalinka, Caitriona M. McEvoy, Ihor Batruch, Tereza Martinu, Julie Van, Olusegun Famure, Stephen C. Juvet, Andrea Bozovic, Alex Boshart, Igor Jurisica

مصطلحات موضوعية: Kidney, Pathology, medicine.medical_specialty, biology, business.industry, urogenital system, Human leukocyte antigen, medicine.disease, Proinflammatory cytokine, medicine.anatomical_structure, Antigen, medicine, biology.protein, Interferon gamma, Tumor necrosis factor alpha, Antibody, business, Acute tubular necrosis, medicine.drug

الوصف: Antibody-mediated rejection (AMR) accounts for >50% of kidney allograft losses. AMR is caused by donor-specific antibodies (DSA) against HLA and non-HLA antigens in the glomeruli and the tubulointerstitium, which together with inflammatory cytokines such as tumor necrosis factor alpha (TNFα) and interferon gamma (IFNɣ), trigger graft injury. Unfortunately, the mechanisms governing cell-specific injury in AMR remain unclear. We studied 30 for-cause kidney biopsies with early AMR, acute cellular rejection or acute tubular necrosis (‘non-AMR’). We laser-captured and microdissected glomeruli and tubulointerstitium, and subjected them to unbiased proteome analysis. 120/2026 glomerular and 180/2399 tubulointerstitial proteins were significantly differentially expressed in AMR vs. non-AMR biopsies (PSIGNIFICANCE STATEMENTAntibody-mediated rejection (AMR) accounts for >50% of kidney allograft loss, and is caused by donor-specific antibodies against HLA antigens, which induce maladaptive responses in the kidney glomeruli and tubulointerstitium. This is the first unbiased proteomics analysis of laser-captured/microdissected glomeruli and tubulointerstitium from 30 indication kidney biopsies with early AMR, acute cellular rejection or acute tubular necrosis. >2,000 proteins were quantified in each compartment. We discovered that basement membrane and extracellular matrix (ECM) proteins were significantly decreased in both AMR compartments. Two ECM-modifying proteins, LGALS1 and GSTO1, were significantly increased in glomeruli and tubulointerstitium, respectively. LGALS1 and GSTO1 were upregulated by anti-HLA antibodies or AMR-related cytokines in primary kidney cells, and may represent therapeutic targets to ameliorate ECM-remodeling in AMR.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bec60729dee325e57bf4dc011885d581Test

المؤلفون: Yuliya Lytvyn, Julie A. Lovshin, Andrew Advani, Mansoor Husain, Bruce A. Perkins, David Z.I. Cherney, Vathany Kulasingam, Daniel C. Cattran, Harindra Rajasekeran, Eleftherios P. Diamandis, Andrea Bozovic, Heather N. Reich

المصدر: American Journal of Physiology-Renal Physiology. 313:F184-F191

مصطلحات موضوعية: Adult, Male, medicine.medical_specialty, Adenosine, Time Factors, Physiology, Sodium, Urinary system, chemistry.chemical_element, 030209 endocrinology & metabolism, Urinalysis, 030204 cardiovascular system & hematology, Kidney, Excretion, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Glucosides, Sodium-Glucose Transporter 2, Predictive Value of Tests, Tandem Mass Spectrometry, Liquid chromatography–mass spectrometry, Internal medicine, Diabetes mellitus, medicine, Humans, Hypoglycemic Agents, Benzhydryl Compounds, Sodium-Glucose Transporter 2 Inhibitors, Type 1 diabetes, Reproducibility of Results, Middle Aged, medicine.disease, Renal Elimination, Diabetes Mellitus, Type 1, Treatment Outcome, medicine.anatomical_structure, Endocrinology, chemistry, Case-Control Studies, Macula densa, Female, Chromatography, Liquid, medicine.drug

الوصف: In experimental models of diabetes, augmented sodium-glucose cotransport-2 (SGLT2) activity diminishes sodium (Na+) delivery at the macula densa. As a result, less vasoconstrictive adenosine is generated, leading to afferent arteriolar vasodilatation and hyperfiltration. The measurement and significance of urinary adenosine in humans has not been examined extensively in states of renal hemodynamic impairment like that of diabetes. Our aim was to validate a method for urine adenosine quantification in humans and perform an exploratory post hoc analysis to determine whether urinary adenosine levels change dynamically in response to natriuresis in patients with type 1 diabetes (T1D) before and after treatment with the SGLT2 inhibitor (SGLT2i) empagliflozin. We hypothesized that SGLT2i, which reduces renal hyperfiltration through increased Na+delivery to the macula densa, would increase urinary adenosine excretion. Urine adenosine corrected for creatinine was measured using our validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method in 40 healthy participants and 40 patients with T1D. In the T1D cohort, measurements were performed during clamped euglycemic and hyperglycemic conditions before and following 8 wk of SGLT2i therapy. Urinary adenosine was detectable in healthy subjects (0.32 ± 0.11 µmol/mmol Cr) and patients with T1D. In response to SGLT2i, urine adenosine increased during clamped hyperglycemia (0.40 ± 0.11 vs. 0.45 ± 0.12 µmol/mmol Cr, P = 0.005). Similar trends were observed during clamped euglycemia ( P = 0.08). In conclusion, SGLT2i increases urinary adenosine excretion under clamped hyperglycemic conditions in patients with T1D. The potentially protective role of SGLT2i against glomerular hyperfiltration and its mediation by adenosine in diabetes merits further study.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ddec741387c76d19c37f19c99fb7f7bdTest
https://doi.org/10.1152/ajprenal.00043.2017Test

المؤلفون: Eshetu G. Atenafu, Andrea Bozovic, Jin-Hyeun Huh, Rita Kwong, Roy Lee, Jack T Seki, Anna Xu

المصدر: The Canadian Journal of Hospital Pharmacy. 70

مصطلحات موضوعية: Gynecology, medicine.medical_specialty, Single use, Stability test, Stem cell mobilization, business.industry, Plerixafor, Environment controlled, Pharmacy, 030204 cardiovascular system & hematology, Single Use Vial, Surgery, 03 medical and health sciences, 0302 clinical medicine, Drug concentration, medicine, Pharmacology (medical), business, Cellules souches, Original Research, 030215 immunology, medicine.drug

الوصف: Background: The addition of the immunostimulant plerixafor to the current standard-of-care regimens of granulocyte colony-stimulating growth factor with or without chemotherapy has improved clinical results in terms of successful stem cell mobilization and the outcomes of stem cell transplant in various settings. With this medical innovation has come an added financial cost for institutions where stem cell transplants are routinely performed, and there may be a further financial burden when the contents of partial vials of the drug are wasted, given that plerixaforvials (Mozobil, Sanofi-Aventis Canada Inc) are currently deemed suitable only for single use.Objective: To determine whether the portion of plerixafor remaining in an opened vial of the Mozobil product after administration of a single dose is chemically stable, by comparison with the original product.Methods: Stability testing of partial drug contents of an opened vial, stored at room temperature or under refrigeration (4°C), was conducted using liquid chromatography–tandem mass spectrometry analysis. The mean concentration of plerixafor (μmol/L), standard deviation, coefficient of variation, and bias were determined on days 2, 3, 11, 17, 24, and 31. Method validation included determination of precision, sensitivity, recovery, dilution linearity, and carryover.Results: Throughout the 4-week testing period, measured plerixafor concentration in aliquots stored at room temperature and under refrigeration, tested in series over time, appeared similar. The mean residual drug concentration after initial opening was slightly, but not significantly, higher for the sample designated for storage at room temperature than the one designated for refrigerated storage (40.4 versus 39.9 μmol/L; p = 0.37).Conclusions: Residual plerixafor after initial opening of a vial of the Mozobil product remained chemically stable for at least 2 weeks both at room temperature and under refrigeration. The results of this study provide in vitro evidence to support multiple uses, instead of single use, of vials of this drug in an aseptic, controlled environment.RÉSUMÉContexte : L’ajout de l’immunostimulant plérixafor aux traitements reconnus comme la norme de soins actuelle quant au facteur de stimulation des colonies de granulocytes, accompagné ou non de chimiothérapie, a amélioré les résultats cliniques de mobilisation des cellules souches et les résultats de greffe de cellules souches dans différents contextes. Cela dit, avec cette innovation médicale vient un poids financier supplémentaire pour les établissements où l’on exécute couramment des greffes de cellules souches. En outre, comme les fioles de plérixafor (Mozobil, Sanofi-Aventis Canada Inc.) sont présentement jugées adéquates pour un usage unique seulement, l’excédent de médicament gaspillé peut représenter une dépense additionnelle.Objectif : Déterminer si ce qui reste de Mozobil dans une fiole ouverte après l’administration d’une dose unique est chimiquement stable comparativement au produit de départ.Méthodes : Une étude de stabilité du contenu partiel d’une fiole de médicament ouverte, entreposé à température ambiante ou conservé au réfrigérateur (4°C), a été réalisée par chromatographie en phase liquide couplée à la spectrométrie de masse en tandem. La concentration moyenne de plérixafor (μmol/L), l’écart-type, le coefficient de variation et le biais ont été établis aux jours 2, 3, 11, 17, 24 et 31. La méthode de validation comprenait la détermination de : la précision, la sensibilité, la récupération, la limite de linéarité et la contamination inter-échantillons.Résultats : Tout au long des quatre semaines d’analyse, les concentrations mesurées des aliquotes de plérixafor, entreposées à température ambiante ou conservées au réfrigérateur et analysées en séries chronologiques, semblaient similaires. Les concentrations moyennes de médicament restant après l’ouverture initiale étaient légèrement plus élevées lorsqu’entreposées à température ambiante (40,4 μmol/L) que lorsque réfrigérées (39,9 μmol/L) (p = 0,37), mais pas de façon significative.Conclusions : Le plérixafor résiduel, après l’ouverture initiale des fioles de Mozobil, demeurait chimiquement stable pendant au moins deux semaines, qu’il soit entreposé à température ambiante ou conservé au réfrigérateur. Les résultats de la présente étude offrent des données in vitro qui soutiennent une utilisation multiple, plutôt qu’un usage unique, des fioles de ce médicament en milieu aseptique contrôlé.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31a4aaed34945052e2e46c64d3681726Test
https://doi.org/10.4212/cjhp.v70i4.1676Test

المؤلفون: Bruce A. Perkins, Harindra Rajasekeran, Michelle Hladunewich, Andrea Bozovic, Daniel C. Cattran, Yuliya Lytvyn, Heather Reichvathany Kulasingam, David Z.I. Cherney

المصدر: Canadian Journal of Diabetes. 40:S64

مصطلحات موضوعية: Type 1 diabetes, medicine.medical_specialty, business.industry, Endocrinology, Diabetes and Metabolism, Urinary system, General Medicine, medicine.disease, Adenosine, Excretion, Endocrinology, Internal medicine, Internal Medicine, medicine, In patient, business, medicine.drug

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::28aee18715ec41461cbf777392b446ddTest
https://doi.org/10.1016/j.jcjd.2016.08.182Test