المؤلفون: Paula Basso Dias, Mariela Regina Dalmarco Ghem, Ana Caroline Martinelli, Anna Carolina Badotti Linhares, Daniel Wasilewski

المصدر: Revista Brasileira de Oftalmologia, Vol 83 (2024)

مصطلحات موضوعية: Amnion/transplantation, Eye burns, Anterior eye segment, Membranes/transplantation, Chemical burns, Ophthalmology, RE1-994

الوصف: ABSTRACT Objective: To analyze the morphological and functional long-term outcomes of amniotic membrane transplantation after ocular surface chemical burns. Methods: This prospective study analyzed 7 patients who suffered from severe ocular surface burn and underwent amniotic membrane transplantation from 2015 to 2020 in Hospital de Clínicas - Universidade Federal do Paraná. Results: Out of the seven patients, six (85.7%) suffered unilateral burn and one (14.3%) suffered bilateral burn. Five of them had alkali burns (71.4%), one had acid burn (14.3%) and one suffered gunpowder fireworks burn (14.3%). Mean age was 29.4 years (±standard deviation 13.3, range 14.0 to 47.0 years). Mean visual acuity at first presentation was 1.83±0.79 logMAR (0.015 decimal) and mean VA after a follow-up of 1 year was 0.85±0.70 logMAR (0.141 decimal). The visual acuity significantly improved from 1.83±0.79 to 0.85±0.70 logMAR (p

وصف الملف: electronic resource

العلاقة: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-72802024000100204&lng=en&tlng=enTest; http://www.scielo.br/pdf/rbof/v83/0034-7280-rbof-83-e0012.pdfTest; https://doaj.org/toc/1982-8551Test

الوصول الحر: https://doaj.org/article/1b8e35e31f504547b03b09381b203de8Test

المؤلفون: Endicott, Luke, Ehresman, Jeff, Tettelbach, William, Forsyth, Allyn, Lee, Bryan S

المصدر: Neurosurgery

مصطلحات موضوعية: cranioplasty, decompressive craniectomies, dehydrated human/amnion chorion membrane, dural scar tissue, intracranial pressure, subdural haematomas, traumatic brain injury, wound, wound care, wound healing, Humans, Amnion (transplantation), Craniotomy (adverse effects), Surgical Flaps, Plastic Surgery Procedures, Tissue Adhesions (surgery, etiology), Postoperative Complications (etiology), Chorion (transplantation)

الوصف: Decompressive craniectomies (DCs) are routinely performed neurosurgical procedures to emergently treat increased intracranial pressure secondary to multiple aetiologies, such as subdural haematoma, epidural haematoma, or malignant oedema in the setting of acute infarction. The DC procedure typically induces epidural fibrosis post-cranial resection, resulting in adherence of the dura to both the brain internally and skin flap externally. This becomes especially problematic in the setting of skull flap replacement for cranioplasty as adherences can lead to bridging vein tear, damage to the underlying brain cortex, and other postoperative complications. Dural adjuvants, which can contribute to decreased rate of adherence formation, can thereby reduce both postoperative cranioplasty complications and operative duration. Dehydrated human amnion/chorion membrane (DHACM) allografts (AMNIOFIX, MIMEDX Group Inc., US) have been shown to reduce the rate of dural scar tissue formation in re-exploration of posterior lumbar interbody fusion operations which require entry into the epidural space. The purpose of this study was to evaluate whether or not the use of DHACM in the setting of emergent craniectomies decreased the rate of dural adhesion formation and subsequent cranioplasty complications. Patients (n=7) who underwent emergent craniectomy and intraoperative placement of DHACM were evaluated during replacement of either an autologous skull cap or a custom-made implant, at which point the degree of adhesions was qualitatively assessed. Placement of DHACM below and on top of the dura resulted in negligible adhesion being found during the defect exposure, and there were no intraoperative complications during cranioplasties. Reported estimated blood loss across the seven patients averaged 64.2ml, total operative time averaged 79.2 minutes, and time dedicated to exposing defect for bone flap placement was <3 minutes.

العلاقة: https://scholar.barrowneuro.org/neurosurgery/1972Test; https://doi.org/10.12968/jowc.2023.32.10.634Test

الإتاحة: https://doi.org/10.12968/jowc.2023.32.10.634Test
https://scholar.barrowneuro.org/neurosurgery/1972Test

المؤلفون: Che-Hsueh Yang, Min-Che Tung, Yi-Sheng Lin, Chao-Yu Hsu, Ivan Akhrymuk, Kok-Tong Tan, Yen-Chuan Ou, Chi-Chien Lin

المصدر: Life; Volume 12; Issue 11; Pages: 1693

مصطلحات موضوعية: animal, anti-infective agents/therapeutic use, amnion/transplantation, cystitis, interstitial/therapy, chorion transplantation

جغرافية الموضوع: agris

الوصف: The study aimed to investigate the therapeutic impact of intravesical instillation of dehydrated human amnion-chorion membrane (HACM) extracts based on the primary pathological feature of interstitial cystitis (IC). We divided 15 female Sprague-Dawley rats into three groups: sham control, IC, and treatment group. IC was induced by 400-µL lipopolysaccharide (1 µg/µL), and it was replaced with normal saline in the sham control group. After IC induction, 300 µL dehydrated HACM extracts (3 mg/kg) were instilled into rats’ urinary bladder weekly for 3 weeks. General histology, inflammatory cytokines, NF-κB, oxidative markers, and western blots results were examined. The urothelial denudation, mast-cell infiltration, and tissues fibrosis were all ameliorated. The elevated TNF-α, IL-1β, IL-6, IL-8, and NF-κB were all down-regulated by dehydrated HACM extracts (p < 0.05). For reactive oxygen species, increased malondialdehyde, decreased superoxide dismutase, and decreased glutathione peroxidase were all reversed (p < 0.05). In apoptosis of IC, elevated Bax and suppressed Bcl-2 were improved (p < 0.05) after instillation. In fibrosis, dysregulated TGFβ/R-Smads/Snail was corrected by the instillation of dehydrated HACM (p < 0.05). In conclusion, dehydrated HACM extracts could be a powerful remedy in treating IC by reconstructing the damaged urothelium, reducing mast-cell infiltration and inflammatory reactions, and ameliorating fibrotic changes.

وصف الملف: application/pdf

العلاقة: Pharmaceutical Science; https://dx.doi.org/10.3390/life12111693Test

الإتاحة: https://doi.org/10.3390/life12111693Test

المؤلفون: Brízido, Margarida Costa e Silva Sena, Lopes, Beatriz de Oliveira, Almeida, Ana Catarina Rodrigues, Miranda, Margarida Maria Domingas Isidoro, Soares, Ícaro Perez

المصدر: Revista Brasileira de Oftalmologia. January 2021 80(3)

مصطلحات موضوعية: Conjunctival diseases/surgery, Amnion/transplantation, Conservative treatment, Fibrin tissue adhesive/therapeutic use

الوصف: Conjunctival concretions are single or clustered lesions frequently found on the palpebral conjunctiva. They are commonly present in older individuals, despite being rarely symptomatic. This case report describes an 83-year-old man with multiple conjunctival concretions, which were surgically treated. The patient was symptomatic on presentation and did not respond to conservative treatment. For this reason, a surgical approach was considered. After wide excision of the conjunctival lesions, a piece of amniotic membrane was fitted using fibrin glue. During follow-up, a markedly improvement in patient's symptoms was observed, along with nearly complete absence of conjunctival concretions. This is the first case report addressing extensive conjunctival concretions with a surgical approach using amniotic membrane. The authors conceived the technique described after noticing the limited clinical options in the literature. This technique was easily performed and achieved satisfactory results.

وصف الملف: text/html

الوصول الحر: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-72802021000300500Test

المؤلفون: Margarida Costa e Silva Sena Brízido, Beatriz de Oliveira Lopes, Ana Catarina Rodrigues Almeida, Margarida Maria Domingas Isidoro Miranda, Ícaro Perez Soares

المصدر: Revista Brasileira de Oftalmologia, Vol 80, Iss 3 (2021)

مصطلحات موضوعية: Conjunctival diseases/surgery, Amnion/transplantation, Conservative treatment, Fibrin tissue adhesive/therapeutic use, Ophthalmology, RE1-994

الوصف: ABSTRACT Conjunctival concretions are single or clustered lesions frequently found on the palpebral conjunctiva. They are commonly present in older individuals, despite being rarely symptomatic. This case report describes an 83-year-old man with multiple conjunctival concretions, which were surgically treated. The patient was symptomatic on presentation and did not respond to conservative treatment. For this reason, a surgical approach was considered. After wide excision of the conjunctival lesions, a piece of amniotic membrane was fitted using fibrin glue. During follow-up, a markedly improvement in patient's symptoms was observed, along with nearly complete absence of conjunctival concretions. This is the first case report addressing extensive conjunctival concretions with a surgical approach using amniotic membrane. The authors conceived the technique described after noticing the limited clinical options in the literature. This technique was easily performed and achieved satisfactory results.

وصف الملف: electronic resource

العلاقة: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-72802021000300500&tlng=enTest; https://doaj.org/toc/1982-8551Test

الوصول الحر: https://doaj.org/article/b7b47f1fe475424eb77075c112c2fd36Test

المساهمون: Seong-Woo Maeng, Tae Yoon Park, Ji Sang Min, Longyu Jin, Kye Il Joo, Woo Chan Park, Hyung Joon Cha, Min, Ji Sang

مصطلحات موضوعية: Amnion* / transplantation, Animals, Conjunctiva, Light, Pterygium* / surgery, Rabbits, Tissue Adhesives, amniotic membrane transplantations, conjunctivas, mussel adhesive proteins, ocular surface reconstructions, visible light-curable bioadhesives

الوصف: The conjunctiva is a thin mucous membrane of the eye. Pterygium, a commonly appearing disease on the ocular surface, requires surgery to excise the conjunctiva to prevent visual deterioration. Recently, transplantation of the amniotic membrane (AM), which is the innermost membrane of the placenta, has been highlighted as an efficient method to cure conjunctiva defects because of its advantages of no side effects compared to mitomycin C treatment and not leaving additional scars on donor site compared to conjunctival autografting. However, to minimize additional damage to the ocular surface by suturing, AM transplantation (AMT) needs to be simplified by using a less invasive, time-saving method. In this work, a visible light-curable protein bioadhesive (named FixLight) for efficient sutureless AMT is applied. FixLight, which is based on bioengineered mussel adhesive protein (MAP), is easily applied between damaged ocular surfaces and transplanted AM, and rapidly cured by harmless blue light activation. Through in vivo evaluation using a rabbit model, the authors demonstrated that FixLight enabled facile, fast, and strong attachment of AM on sclera and promoted ocular surface reconstruction with good biocompatibility. Thus, FixLight can be successfully used as a promising clinical bioadhesive in opthalmological surgeries that require sutureless and rapid operation. ; restriction

العلاقة: ADVANCED HEALTHCARE MATERIALS; J00042; OAK-2022-04969; https://ir.ymlib.yonsei.ac.kr/handle/22282913/190842Test; T9992022253; ADVANCED HEALTHCARE MATERIALS, Vol.10(13), 2021-07

الإتاحة: https://doi.org/10.1002/adhm.202100100Test
https://ir.ymlib.yonsei.ac.kr/handle/22282913/190842Test

المؤلفون: Goktas, Seniz Engur, Katircioglu, Yasemin, Celik, Tuba, Ornek, Firdevs

المصدر: Arquivos Brasileiros de Oftalmologia. August 2017 80(4)

مصطلحات موضوعية: Conjunctival neoplasm surgery, Squamous cell carcinoma, Amnion transplantation, Cryotherapy

الوصف: Purpose: To evaluate the clinical results of patients treated by amniotic membrane transplantation (AMT) following excision of conjunctival and limbal tumors. Methods: A total of 14 eyes of 14 patients who underwent AMT after total lesion-free tumor excision and perilesional cryotherapy were evaluated. Results: The excised tumors comprised 7 conjunctival intraepithelial neoplasia, 5 conjunctival nevus, 1 primary acquired melanosis, and 1 squamous cell carcinoma. Limbus was involved in 10 cases, whereas cornea was involved in 6 cases. The average measurement of the tumor base was 14.8 mm (range 6-20 mm, SD 16 mm). The mean follow-up time period was 17.5 months (range 6 -60 months, SD 20 months). Complete healing occurred in eight eyes, but limbal cell deficiency developed in two eyes. Four cases had recurrence and were treated with the same surgical and medical procedures; during follow-up after recurrence, superficial peripheral vascularization and corneal scar were noted to have developed. Conclusions: AMT was effective for reconstruction of tissue defect after excision and cryotherapy of limbal and conjunctival tumors. In most of these cases, complete healing was achieved with a smooth, stable, and translucent surface.

وصف الملف: text/html

الوصول الحر: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492017000400242Test

المؤلفون: Goktas S.E., Katircioglu Y., Celik T., Ornek F.

المساهمون: Goktas S.E., Katircioglu Y., Celik T., Ornek F., Zonguldak Bülent Ecevit Üniversitesi

مصطلحات موضوعية: Amnion transplantation, Conjunctival neoplasm surgery, Cryotherapy, Squamous cell carcinoma

الوصف: Purpose: To evaluate the clinical results of patients treated by amniotic membrane transplantation (AMT) following excision of conjunctival and limbal tumors. Methods: A total of 14 eyes of 14 patients who underwent AMT after total lesion-free tumor excision and perilesional cryotherapy were evaluated. Results: The excised tumors comprised 7 conjunctival intraepithelial neoplasia, 5 conjunctival nevus, 1 primary acquired melanosis, and 1 squamous cell carcinoma. Limbus was involved in 10 cases, whereas cornea was involved in 6 cases. The average measurement of the tumor base was 14.8 mm (range 6-20 mm, SD 16 mm). The mean follow-up time period was 17.5 months (range 6 -60 months, SD 20 months). Complete healing occurred in eight eyes, but limbal cell deficiency developed in two eyes. Four cases had recurrence and were treated with the same surgical and medical procedures; during follow-up after recurrence, superficial peripheral vascularization and corneal scar were noted to have developed. Conclusions: AMT was effective for reconstruction of tissue defect after excision and cryotherapy of limbal and conjunctival tumors. In most of these cases, complete healing was achieved with a smooth, stable, and translucent surface.

العلاقة: Arquivos Brasileiros de Oftalmologia; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; 242; 246; 80; https://dx.doi.org/10.5935/0004-2749.20170059Test; https://hdl.handle.net/20.500.12628/7706Test

الإتاحة: https://doi.org/20.500.12628/770610.5935/0004-2749.20170059Test
https://hdl.handle.net/20.500.12628/7706Test

المؤلفون: Che-Hsueh Yang, Min-Che Tung, Yi-Sheng Lin, Chao-Yu Hsu, Ivan Akhrymuk, Kok-Tong Tan, Yen-Chuan Ou, Chi-Chien Lin

المصدر: Life; Volume 12; Issue 11; Pages: 1693

مصطلحات موضوعية: animal, anti-infective agents/therapeutic use, amnion/transplantation, cystitis, interstitial/therapy, chorion transplantation, Space and Planetary Science, Paleontology, General Biochemistry, Genetics and Molecular Biology, Ecology, Evolution, Behavior and Systematics

الوصف: The study aimed to investigate the therapeutic impact of intravesical instillation of dehydrated human amnion-chorion membrane (HACM) extracts based on the primary pathological feature of interstitial cystitis (IC). We divided 15 female Sprague-Dawley rats into three groups: sham control, IC, and treatment group. IC was induced by 400-µL lipopolysaccharide (1 µg/µL), and it was replaced with normal saline in the sham control group. After IC induction, 300 µL dehydrated HACM extracts (3 mg/kg) were instilled into rats’ urinary bladder weekly for 3 weeks. General histology, inflammatory cytokines, NF-κB, oxidative markers, and western blots results were examined. The urothelial denudation, mast-cell infiltration, and tissues fibrosis were all ameliorated. The elevated TNF-α, IL-1β, IL-6, IL-8, and NF-κB were all down-regulated by dehydrated HACM extracts (p < 0.05). For reactive oxygen species, increased malondialdehyde, decreased superoxide dismutase, and decreased glutathione peroxidase were all reversed (p < 0.05). In apoptosis of IC, elevated Bax and suppressed Bcl-2 were improved (p < 0.05) after instillation. In fibrosis, dysregulated TGFβ/R-Smads/Snail was corrected by the instillation of dehydrated HACM (p < 0.05). In conclusion, dehydrated HACM extracts could be a powerful remedy in treating IC by reconstructing the damaged urothelium, reducing mast-cell infiltration and inflammatory reactions, and ameliorating fibrotic changes. Published version

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9579788e1a6d427d54e3040df0b16c0eTest
https://hdl.handle.net/10919/112294Test

المؤلفون: Paris, Fabiana dos Santos, Gonçalves, Eliana Domingues, Morales, Maira Saad Ávila, Kanecadan, Liliane Andrade Almeida, Campos, Mauro Silveira de Queiroz, Gomes, José Álvaro Pereira, Allemann, Norma, Sato, Elcio Hideo

المصدر: Arquivos Brasileiros de Oftalmologia. December 2014 77(6)

مصطلحات موضوعية: Corneal edema, Corneal endothelial cell loss, Corneal pachymetry, Cornea/ultrasound, Amnion/transplantation, Biological dressings, Palliative care

الوصف: Purpose: To describe quantitative and qualitative features of eyes with advanced bullous keratopathy assessed using ultrasound biomicroscopy, before and after anterior stromal puncture (ASP) or amniotic membrane transplantation (AMT) procedures to relieve chronic pain. Methods: The present descriptive comparative study included 40 eyes of 40 patients with chronic intermittent pain due to bullous keratopathy who were randomly assigned to one of the two treatments (AMT or ASP). Ultrasound biomicroscopy (Humphrey, UBM 840, 50 MHz transducer, immersion technique) was used, and a questionnaire about pain intensity was completed preoperatively and postoperatively at days 90 and 180, respectively. Exclusion criteria were age<18 years, presence of concurrent infection, ocular hypertension, and absence of pain. Results: In a 180-day follow-up, the AMT group exhibited mean central corneal thickness (CCT), 899.4 µm preoperatively and 1122.5 µm postoperatively (p<0.001); mean epithelial thickness (ET), 156.4 µm preoperatively and 247.8 µm postoperatively (p<0.001); and mean stromal thickness (ST), 742.9 µm preoperatively and 826.3 µm postoperatively (p=0.005). The ASP group exhibited mean CCT, 756.7 µm preoperatively and 914.8 µm postoperatively (p<0.001); mean ET, 102.1 µm preoperatively and 245.2 µm postoperatively (p<0.001); and mean ST, 654.6 µm preoperatively and 681.5 µm postoperatively (p<0.999). Correlations between CCT and pain intensity in the AMT group (p=0.209 pre- and postoperatively) and the ASP group (p=0.157 preoperatively and p=0.426 at the 180-day follow-up) were not statistically significant. Epithelial and stromal edema, Descemet’s membrane folds, epithelial bullae, and the presence of interface fluid were frequently observed qualitative features. Conclusion: CCT increased over time in both groups. The magnitude of CCT did not correlate with pain intensity in the sample studied. The presence of interface fluid was a qualitative feature specifically found in some patients who underwent AMT.

وصف الملف: text/html

الوصول الحر: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492014000600014Test