المؤلفون: Alkharaan, Hassan, Bayati, Shaghayegh, Hellström, Cecilia, Aleman, Soo, Olsson, Annika, Lindahl, Karin, Bogdanovic, Gordana, Healy, Katie, Tsilingaridis, Georgios, De Palma, Patricia, Hober, Sophia, Professor, 1965, Månberg, Anna, 1985, Nilsson, Peter, Pin, Elisa, Chen, Margaret Sallberg

المصدر: Journal of Infectious Diseases. 224(3):407-414

مصطلحات موضوعية: antibody, convalescence, COVID-19, immunoassay, saliva, serology

الوصف: Background. Declining humoral immunity in coronavirus disease 2019 (COVID-19) patients and possible reinfection have raised concern. Mucosal immunity, particularly salivary antibodies, may be short lived although long-term studies are lacking. Methods. Using a multiplex bead-based array platform, we investigated antibodies specific to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteins in 256 saliva samples from convalescent patients 1-9 months after symptomatic COVID-19 (n = 74, cohort 1), undiagnosed individuals with self-reported questionnaires (n = 147, cohort 2), and individuals sampled prepandemic (n = 35, cohort 3). Results. Salivary IgG antibody responses in cohort 1 (mainly mild COVID-19) were detectable up to 9 months postrecovery, with high correlations between spike and nucleocapsid specificity. At 9 months, IgG remained in blood and saliva in most patients. Salivary IgA was rarely detected at this time point. In cohort 2, salivary IgG and IgA responses were significantly associated with recent history of COVID-19-like symptoms. Salivary IgG tolerated temperature and detergent pretreatments. Conclusions. Unlike SARS-CoV-2 salivary IgA that appeared short lived, specific saliva IgG appeared stable even after mild COVID-19, as for blood serology. This noninvasive saliva-based SARS-CoV-2 antibody test with home self-collection may be a complementary alternative to conventional blood serology.

وصف الملف: print

الوصول الحر: https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-300852Test
https://doi.org/10.1093/infdis/jiab256Test

المؤلفون: Healy, Katie, Pin, Elisa, Chen, Puran, Söderdahl, Gunnar, Nowak, Piotr, Mielke, Stephan, Hansson, Lotta, Bergman, Peter, Smith, C.I. Edvard, Ljungman, Per, Valentini, Davide, Blennow, Ola, Österborg, Anders, Gabarrini, Giorgio, Al-Manei, Khaled, Alkharaan, Hassan, Sobkowiak, Michał Jacek, Yousef, Jamil, Mravinacova, Sara, Cuapio, Angelica, Xu, Xinling, Akber, Mira, Loré, Karin, Hellström, Cecilia, Muschiol, Sandra, Bogdanovic, Gordana, Buggert, Marcus, Ljunggren, Hans-Gustaf, Hober, Sophia, Nilsson, Peter, Aleman, Soo, Sällberg Chen, Margaret

مصطلحات موضوعية: antibody, cancer, COVID-19, HIV, immunodeficiency, saliva, serum, Translation to patients, transplantation, vaccination, Infectious Medicine, Infektionsmedicin

الوصف: Background: Immunocompromised individuals are highly susceptible to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Whether vaccine-induced immunity in these individuals involves oral cavity, a primary site of infection, is presently unknown. Methods: Immunocompromised patients (n = 404) and healthy controls (n = 82) participated in a prospective clinical trial (NCT04780659) encompassing two doses of the mRNA BNT162b2 vaccine. Primary immunodeficiency (PID), secondary immunodeficiencies caused by human immunodeficiency virus (HIV) infection, allogeneic hematopoietic stem cell transplantation (HSCT)/chimeric antigen receptor T cell therapy (CAR-T), solid organ transplantation (SOT), and chronic lymphocytic leukemia (CLL) patients were included. Salivary and serum immunoglobulin G (IgG) reactivities to SARS-CoV-2 spike were measured by multiplex bead-based assays and Elecsys anti-SARS-CoV-2 S assay. Findings: IgG responses to SARS-CoV-2 spike antigens in saliva in HIV and HSCT/CAR-T groups were comparable to those of healthy controls after vaccination. The PID, SOT, and CLL patients had weaker responses, influenced mainly by disease parameters or immunosuppressants. Salivary responses correlated remarkably well with specific IgG titers and the neutralizing capacity in serum. Receiver operating characteristic curve analysis for the predictive power of salivary IgG yielded area under the curve (AUC) = 0.95 and positive predictive value (PPV) = 90.7% for the entire cohort after vaccination. Conclusions: Saliva conveys vaccine responses induced by mRNA BNT162b2. The predictive power of salivary spike IgG makes it highly suitable for screening vulnerable groups for revaccination.

وصف الملف: application/pdf

العلاقة: 2022, 3:2, s. 137-153.e3; Med, 2666-6359, 2022, 3:2, s. 137-153.e3; http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-192375Test; PMID 35075450; ISI:000758830400012; Scopus 2-s2.0-85123922478

الإتاحة: https://doi.org/10.1016/j.medj.2022.01.001Test
http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-192375Test

المؤلفون: Gaiser, Rogier Aäron, Pessia, Alberto, Ateeb, Zeeshan, Davanian, Haleh, Moro, Carlos Fernández, Alkharaan, Hassan, Healy, Katie, Ghazi, Sam, Arnelo, Urban, Valente, Roberto, Velagapudi, Vidya, Chen, Margaret Sällberg, Del Chiaro, Marco

المصدر: Scientific Reports ; volume 12, issue 1 ; ISSN 2045-2322

مصطلحات موضوعية: Multidisciplinary

الإتاحة: https://doi.org/10.1038/s41598-022-08527-zTest
https://www.nature.com/articles/s41598-022-08527-z.pdfTest
https://www.nature.com/articles/s41598-022-08527-zTest

المؤلفون: Alkharaan, Hassan, Bayati, Shaghayegh, Hellström, Cecilia, Aleman, Soo, Olsson, Annika, Lindahl, Karin, Bogdanovic, Gordana, Healy, Katie, Tsilingaridis, Georgios, De Palma, Patricia, Hober, Sophia, Månberg, Anna, Nilsson, Peter, Pin, Elisa, Sällberg Chen, Margaret

المساهمون: Region Stockholm, Knut and Alice Wallenberg Foundation, Science for Life Laboratory, Erling-Persson Family Foundation

المصدر: The Journal of Infectious Diseases ; volume 227, issue 4, page 603-603 ; ISSN 0022-1899 1537-6613

مصطلحات موضوعية: Infectious Diseases, Immunology and Allergy

الإتاحة: https://doi.org/10.1093/infdis/jiac121Test
https://academic.oup.com/jid/article-pdf/227/4/603/49178343/jiac121.pdfTest

المؤلفون: Alkharaan, Hassan

المساهمون: Karolinska Institutet, Dept of Dental Medicine, Publisher, Karolinska Institutet, Inst för odontologi, Publisher

مصطلحات موضوعية: MEDICAL AND HEALTH SCIENCES, MEDICIN OCH HÄLSOVETENSKAP

الوصف: Background and objectives: Etiological factors and accurate diagnostic biomarkers have remained elusive to pancreatic cancer, a disease with > 90% five-years mortality rate. The recent understanding of microbiome interaction with host organs has opened new research avenues on the potential role of microbiota in the tumor microenvironment. The objectives in Study I and II aim to investigate molecular and microbiome related biomarkers in plasma, saliva, pancreatic fluid and tissues from patient groups diagnosed with pancreatic cystic neoplasms, and their correlation with pancreatic neoplastic grade.The COVID-19 outbreak occurred in an unprecedented transmission rate which necessitate diagnostic biomarkers to manage the pandemic. The objective in Study III aims to investigate the potential use of saliva as a non-invasive approach for assessment of immune exposure to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).Results: Study I shows that elevated bacterial 16S DNA copies and IL-1β levels in pancreas cysts correlate with high neoplastic grade. Analysis of the intracystic pancreas microbiome shows co-occurrence and enrichment of oral bacterial species. Bacteria DNA level in pancreas is also associated to prior exposure to invasive endoscopic procedures.Study II shows that elevated plasma and salivary antibody reactivity to oral pathogens (particularly F. nucleatum or Fap2 of F. nucleatum) is associated with intraductal papillary mucinous neoplasm (IPMN) diagnosis showing high-grade dysplasia or invasive cancer.Study III shows that salivary antibody reactivity to SARS-CoV-2 spike and capsid antigens persist up to 9 months after mild COVID-19 with a new multiplex antibody assay. Presence of specific salivary antibodies also correlates to COVID-19 like symptoms in a second undiagnosed cohort. The virus-specific IgG in saliva appears stable and tolerates temperature and detergent pre-treatments.Conclusion: Collectively, the results indicate that oral microbes have a role in disease progression of pancreatic cystic neoplasms. Reducing the inflammatory microbiome may be a potential therapeutic strategy. The salivary antibody testing against oral pathogens holds interesting promise for early identification of high-risk pancreatic tumors. Furthermore, salivary antibody testings on a multiplex platform perform well and can support immune diagnostics of COVID-19. By combining at-home sample collection and the multiplex strategy, salivary diagnostics can be a sensitive and non-invasive alternative to conventional tests currently available

وصف الملف: electronic

الوصول الحر: http://hdl.handle.net/10616/47605Test

المؤلفون: Alkharaan, Hassan, Lu, Liyan, Gabarrini, Giorgio, Halimi, Asif, Ateeb, Zeeshan, Sobkowiak, Michał J., Davanian, Haleh, Fernández Moro, Carlos, Jansson, Leif, Del Chiaro, Marco, Özenci, Volkan, Sällberg Chen, Margaret

المساهمون: Cancerfonden, Radiumhemmets Forskningsfonder, Karolinska Institutet

المصدر: Frontiers in Immunology ; volume 11 ; ISSN 1664-3224

الإتاحة: https://doi.org/10.3389/fimmu.2020.02003Test

المؤلفون: Gaiser, Rogier Aäron, Pessia, Alberto, Ateeb, Zeeshan, Davanian, Haleh, Fernández Moro, Carlos, Alkharaan, Hassan, Healy, Katie, Ghazi, Sam, Arnelo, Urban, Valente, Roberto, Velagapudi, Vidya, Sällberg Chen, Margaret, Del Chiaro, Marco

المساهمون: Institute for Molecular Medicine Finland, Helsinki Institute of Life Science HiLIFE, University of Helsinki

مصطلحات موضوعية: 3122 Cancers, Pancreatic cancer, Intraductal papillary mucinous neoplasms, Serous cystic neoplasm, Pancreatic cyst

الوصف: Pancreatic cystic neoplasms (PCNs) are a highly prevalent disease of the pancreas. Among PCNs, Intraductal Papillary Mucinous Neoplasms (IPMNs) are common lesions that may progress from low-grade dysplasia (LGD) through high-grade dysplasia (HGD) to invasive cancer. Accurate discrimination of IPMN-associated neoplastic grade is an unmet clinical need. Targeted (semi)quantitative analysis of 100 metabolites and >1000 lipid species were performed on peri-operative pancreatic cyst fluid and pre-operative plasma from IPMN and serous cystic neoplasm (SCN) patients in a pancreas resection cohort (n = 35). Profiles were correlated against histological diagnosis and clinical parameters after correction for confounding factors. Integrated data modeling was used for group classification and selection of the best explanatory molecules. Over 1000 different compounds were identified in plasma and cyst fluid. IPMN profiles showed significant lipid pathway alterations compared to SCN. Integrated data modeling discriminated between IPMN and SCN with 100% accuracy and distinguished IPMN LGD or IPMN HGD and invasive cancer with up to 90.06% accuracy. Free fatty acids, ceramides, and triacylglycerol classes in plasma correlated with circulating levels of CA19-9, albumin and bilirubin. Integrated metabolomic and lipidomic analysis of plasma or cyst fluid can improve discrimination of IPMN from SCN and within PMNs predict the grade of dysplasia. ; Peer reviewed

وصف الملف: application/pdf

العلاقة: Gaiser , R A , Pessia , A , Ateeb , Z , Davanian , H , Fernández Moro , C , Alkharaan , H , Healy , K , Ghazi , S , Arnelo , U , Valente , R , Velagapudi , V , Sällberg Chen , M & Del Chiaro , M 2019 , ' Integrated targeted metabolomic and lipidomic analysis: A novel approach to classifying early cystic precursors to invasive pancreatic cancer ' , Scientific Reports , vol. 9 , 10208 . https://doi.org/10.1038/s41598-019-46634-6Test; 6e89941b-045f-4742-aaf9-6dd3b748d4c7; http://hdl.handle.net/10138/304929Test; 000475467800051

الإتاحة: http://hdl.handle.net/10138/304929Test

المؤلفون: Gaiser, Rogier Aäron, Halimi, Asif, Alkharaan, Hassan, Lu, Liyan, Davanian, Haleh, Healy, Katie, Hugerth, Luisa W, Ateeb, Zeeshan, Valente, Roberto, Fernández Moro, Carlos, Del Chiaro, Marco, Sällberg Chen, Margaret

مصطلحات موضوعية: Pancreas

الوصف: Objectives Intraductal papillary mucinous neoplasms (IPMNs) are pancreatic cysts that can progress to invasive pancreatic cancer. Associations between oncogenesis and oral microbiome alterations have been reported. This study aims to investigate a potential intracystic pancreatic microbiome in a pancreatic cystic neoplasm (PCN) surgery patient cohort. Design Paired cyst fluid and plasma were collected at pancreatic surgery from patients with suspected PCN (n=105). Quantitative and qualitative assessment of bacterial DNA by qPCR, PacBio sequencing (n=35), and interleukin (IL)-1β quantification was performed. The data were correlated to diagnosis, lesion severity and clinical and laboratory profile, including proton-pump inhibitor (PPI) usage and history of invasive endoscopy procedures. Results Intracystic bacterial 16S DNA copy number and IL-1β protein quantity were significantly higher in IPMN with high-grade dysplasia and IPMN with cancer compared with non-IPMN PCNs. Despite high interpersonal variation of intracystic microbiota composition, bacterial network and linear discriminant analysis effect size analyses demonstrated co-occurrence and enrichment of oral bacterial taxa including Fusobacterium nucleatum and Granulicatella adiacens in cyst fluid from IPMN with high-grade dysplasia. The elevated intracystic bacterial DNA is associated with, but not limited to, prior exposure to invasive endoscopic procedures, and is independent from use of PPI and antibiotics. Conclusions Collectively, these findings warrant further investigation into the role of oral bacteria in cystic precursors to pancreatic cancer and have added values on the aetiopathology as well as the management of pancreatic cysts.

وصف الملف: text/html

العلاقة: http://gut.bmj.com/cgi/content/short/68/12/2186Test; http://dx.doi.org/10.1136/gutjnl-2018-317458Test

الإتاحة: https://doi.org/10.1136/gutjnl-2018-317458Test
http://gut.bmj.com/cgi/content/short/68/12/2186Test

المؤلفون: Alsakr, Abdulaziz, Gufran, Khalid, Alqahtani, Abdullah Saad, Alkharaan, Hassan, Abushanan, Alwaleed, Alnufaiy, Banna, Alkhaldi, Abdullah, Alshammari, Tareq, Alanazi, Muhannad

المصدر: Medicina (1010660X); Jul2023, Vol. 59 Issue 7, p1284, 12p

مصطلحات موضوعية: FEAR of dentists, DENTAL clinics, DENTAL care utilization, MANN Whitney U Test, DENTAL scaling, DENTAL care

مستخلص: Background and Objectives: This study aimed to assess the dental anxiety of patients using the modified dental anxiety scale (MDAS) questionnaire along with examining the possible relationship between dental anxiety and sociodemographic factors. Materials and Methods: The MDAS questionnaire was used to assess the anxiety level of the patients which included a total of five questions and five options to respond to each question. MDAS questionnaire was filled out by all the patients before the dental treatment. After finishing the treatment, all the patients were given a post-treatment questionnaire to assess their anxiety levels after treatment. Descriptive statistics were performed for measuring the frequency of responses. Mann-Whitney U test was used to compare the anxiety between the gender. The chi-square test was used to identify the distribution of pre-treatment with gender and post-treatment questionnaire with gender and treatment modalities. Linear regression was used to identify the association between clinical variables and different levels of anxiety. Results: A total of 115 patients including 59 males and 56 females with a mean age of 35 ± 11.34 years were included in the current study. Female patients were significantly more anxious about dental treatment compared to male patients. Linear regression showed that age and gender have a significant association with the pre-treatment anxiety level; however, types of treatment is not associated with the MDAS. Anxiety levels decreased for the majority of the patients after the treatment and types of treatment did not show any differences with the post-treatment anxiety level. Conclusions: Age and gender play an important role in dental anxiety; however, types of treatment are not associated with pre-treatment and post-treatment dental anxiety. [ABSTRACT FROM AUTHOR]

: Copyright of Medicina (1010660X) is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Alkharaan, Hassan, Al-Qarni, Hatem, Aldosari, Muath A., Alsaloum, Mohammed, Aldakheel, Ghada, Alenazi, Mohammed W., Alharbi, Naif Khalaf

المصدر: Vaccines; Apr2023, Vol. 11 Issue 4, p744, 11p

مصطلحات موضوعية: ANTIBODY formation, COVID-19 pandemic, COVID-19 vaccines, BOOSTER vaccines, ORAL vaccines

مستخلص: Background: To date, little is known about the salivary mucosal immune response following different COVID-19 vaccine types or after a booster (3rd) dose of the BNT162b2 (BNT) vaccine. Methods: A total of 301 saliva samples were collected from vaccinated individuals and arranged into two cohorts: cohort 1 (n = 145), samples from individuals who had received two doses against SARS-CoV-2; cohort 2 (n = 156), samples from individuals who had received a booster of BNT vaccine. Cohorts 1 and 2 were sub-stratified into three groups based on the types of first and second doses (homologous BNT/BNT, homologous ChAdOx1/ChAdOx1, or heterologous BNT/ChAdOx1vaccinations). Salivary immunoglobulin G (IgG) response to SARS-CoV-2 spike glycoprotein was measured by ELISA, and clinical demographic data were collected from hospital records or questionnaires. Results: Salivary IgG antibody responses against different vaccines, whether homologous or heterogeneous vaccination regimens, showed similar levels in cohorts 1 and 2. Compiling all groups in cohort 1 and 2 showed significant, albeit weak, negative correlations between salivary IgG levels and time (r = −0.2, p = 0.03; r = −0.27, p = 0.003, respectively). In cohort 2, the durability of salivary IgG after a booster dose of BNT162b2 significantly dropped after 3 months compared to the <1 month and 1–3 months groups. Conclusions: Different COVID-19 vaccine types and regimens elicit similar salivary anti-SARS-CoV-2 IgG with modest waning over time. Boosting with BNT162b2 vaccine did not produce an evident increase in mucosal IgG response whereby COVID-19 recovered subjects show higher salivary IgG than naive, post-vaccination subjects. The ChAdOx1/ChAdOx1 regimen showed better correlation between salivary IgG levels and durability. These findings highlight the importance of developing oral or intra-nasal vaccines to induce stronger mucosal immunity. [ABSTRACT FROM AUTHOR]

: Copyright of Vaccines is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)