المؤلفون: Ivana Giangrieco, Maria Antonietta Ciardiello, Maurizio Tamburrini, Lisa Tuppo, Adriano Mari, Claudia Alessandri

المصدر: Foods, Vol 13, Iss 5, p 790 (2024)

مصطلحات موضوعية: sensitisation, tissue permeabilisation, plant food, mite proteases, tight junction, gastrointestinal digestion, Chemical technology, TP1-1185

الوصف: Papain-like cysteine proteases are widespread and can be detected in all domains of life. They share structural and enzymatic properties with the group’s namesake member, papain. They show a broad range of protein substrates and are involved in several biological processes. These proteases are widely exploited for food, pharmaceutical, chemical and cosmetic biotechnological applications. However, some of them are known to cause allergic reactions. In this context, the objective of this review is to report an overview of some general properties of papain-like cysteine proteases and to highlight their contributions to allergy reactions observed in humans. For instance, the literature shows that their proteolytic activity can cause an increase in tissue permeability, which favours the crossing of allergens through the skin, intestinal and respiratory barriers. The observation that allergy to PLCPs is mostly detected for inhaled proteins is in line with the reports describing mite homologs, such as Der p 1 and Der f 1, as major allergens showing a frequent correlation between sensitisation and clinical allergic reactions. In contrast, the plant food homologs are often digested in the gastrointestinal tract. Therefore, they only rarely can cause allergic reactions in humans. Accordingly, they are reported mainly as a cause of occupational diseases.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2304-8158/13/5/790Test; https://doaj.org/toc/2304-8158Test

الوصول الحر: https://doaj.org/article/a4c476544231417e9d74dfea541b2509Test

المؤلفون: Enrica Roccotiello, Elena Nicosia, Lorenzo Pierdonà, Pietro Marescotti, Maria Antonietta Ciardiello, Ivana Giangrieco, Adriano Mari, Danila Zennaro, Denise Dozza, Michele Brancucci, Mauro Mariotti

المصدر: Scientific Reports, Vol 12, Iss 1, Pp 1-15 (2022)

مصطلحات موضوعية: Medicine, Science

الوصف: Abstract Vegetables represent a major source of Ni exposure. Environmental contamination and cultural practices can increase Ni amount in tomato posing significant risk for human health. This work assesses the tomato (Solanum lycopersicum L.) response to Ni on the agronomic yield of fruits and the related production of allergens. Two cultivars were grown in pots amended with Ni 0, 30, 60, 120, and 300 mg kg−1, respectively. XRF and ICP-MS analyses highlighted the direct increase of fruit Ni content compared to soil Ni, maintaining a stable biomass. Leaf water content increased at Ni 300 mg kg−1. Total protein content and individual allergenic components were investigated using biochemical (RP-HPLC and N-terminal amino acid sequencing) and immunological (inhibition tests of IgE binding by SPHIAa assay on the FABER testing system) methodologies. Ni affected the fruit tissue concentration of pathogenesis-related proteins and relevant allergens (LTP, profilin, Bet v 1-like protein and TLP). This study elucidates for the first time that tomato reacts to exogenous Ni, uptaking the metal while changing its allergenic profiles, with potential double increasing of exposure risks for consumers. This evidence highlighted the importance of adequate choice of low-Ni tomato cultivars and practices to reduce Ni uptake by potentially contaminated matrices.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2045-2322Test

الوصول الحر: https://doaj.org/article/aeeec70a0f474272ab06d4bdfb9ebf54Test

المؤلفون: Ivana Giangrieco, Maria Antonietta Ciardiello, Maurizio Tamburrini, Lisa Tuppo, Chiara Rafaiani, Adriano Mari, Claudia Alessandri

المصدر: Foods, Vol 12, Iss 15, p 2852 (2023)

مصطلحات موضوعية: papain-like cysteine protease, allergy, safety, protein primary structure, food industry, Act d 1, Chemical technology, TP1-1185

الوصف: Several plant papain-like cysteine proteases are exploited by the food, cosmetic, pharmaceutical and textile industries. However, some of these enzymes can cause allergic reactions. In this context, we investigated the frequency of sensitization and allergic reactions to some fruit and/or latex cysteine proteases, which are used as additives by the food industry to improve and modify the quality of their products. The FABER test was used to analyse the patients‘ sensitization towards five plants and, for comparison, two homologous mite cysteine proteases. In an Italian population of 341 allergic patients, 133 (39%) had IgE specific for at least one of the seven cysteine proteases under investigation. Most of the patients were IgE positive for Der p 1 and/or Der f 1 (96.38%) reported a clinical history suggestive of respiratory allergy to mites, whereas none of the subjects sensitized to the homologs from papaya, pineapple and fig reported allergy symptoms following ingestion of these foods. Only one patient referred symptoms from ingesting kiwifruit. Therefore, the obtained results showed that sensitization to the fruit enzymes was only rarely concomitant with allergic reactions. These observations, together with the literature reports, suggest that the allergy to plant papain-like cysteine proteases might mainly be an occupational disease.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2304-8158/12/15/2852Test; https://doaj.org/toc/2304-8158Test

الوصول الحر: https://doaj.org/article/f39d2007ca4647929b242202ceefddfaTest

المؤلفون: Christopher Stanly, Hyoseon Kim, Giuseppe Antonucci, Immacolata Fiume, Michele Guescini, Kwang Pyo Kim, Maria Antonietta Ciardiello, Ivana Giangrieco, Adriano Mari, Gabriella Pocsfalvi

المصدر: Frontiers in Bioengineering and Biotechnology, Vol 9 (2021)

مصطلحات موضوعية: strawberry, nanovesicles, allergens, proteomics, sequence similarity, Biotechnology, TP248.13-248.65

الوصف: Background: Nanometer-sized membrane-surrounded vesicles from different parts of plants including fruits are gaining increasing attention due to their anti-inflammatory and anticancer effects demonstrated by in vitro and in vivo studies, and as nanovectors for molecular delivery of exogenous substances. These nanomaterials are very complex and contain a diverse arsenal of bioactive molecules, such as nucleic acids, proteins, and lipids. Our knowledge about the transport of allergens in vesicles isolated from plant food is limited today.Methods: Here, to investigate the allergenicity of strawberry-derived microvesicles (MVs), nanovesicles (NVs), and subpopulations of NV, we have set up a multidisciplinary approach. The strategy combines proteomics-based protein identification, immunological investigations, bioinformatics, and data mining to gain biological insights useful to evaluate the presence of potential allergens and the immunoglobulin E (IgE) inhibitory activity of vesicle preparations.Results: Immunological test showed that several proteins of strawberry-derived vesicles compete for IgE binding with allergens spotted on the FABER biochip. This includes the known strawberry allergens Fra a 1, Fra a 3, and Fra a 4, and also other IgE-binding proteins not yet described as allergens in this food, such as gibberellin-regulated proteins, 2S albumin, pectate lyase, and trypsin inhibitors. Proteomics identified homologous sequences of the three strawberry allergens and their isoforms in total protein extract (TPE) but only Fra a 1 and Fra a 4 in the vesicle samples. Label-free quantitative proteomic analysis revealed no significant enrichment of these proteins in strawberry vesicles with respect to TPE.Conclusion: Immunological tests and bioinformatics analysis of proteomics data sets revealed that MVs and NVs isolated from strawberries can carry functional allergens their isoforms as well as proteins potentially allergenic based on their structural features. This should be considered when these new nanomaterials are used for human nutraceutical or biomedical applications.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fbioe.2021.760730/fullTest; https://doaj.org/toc/2296-4185Test

الوصول الحر: https://doaj.org/article/08ca869c43f04d9ea222ffa979a0588aTest

المؤلفون: Claudia Alessandri, Rosetta Ferrara, Maria Livia Bernardi, Danila Zennaro, Lisa Tuppo, Ivana Giangrieco, Teresa Ricciardi, Maurizio Tamburrini, Maria Antonietta Ciardiello, Adriano Mari

المصدر: Clinical and Translational Allergy, Vol 10, Iss 1, Pp 1-18 (2020)

مصطلحات موضوعية: Oral allergy syndrome, Pollen-food allergy syndrome, Food allergy, Oral cavity, Allergenic molecules, Isoallergens, Immunologic diseases. Allergy, RC581-607

الوصف: Abstract Oral allergy syndrome (OAS) is one of the most common IgE-mediated allergic reactions. It is characterized by a number of symptoms induced by the exposure of the oral and pharyngeal mucosa to allergenic proteins belonging to class 1 or to class 2 food allergens. OAS occurring when patients sensitized to pollens are exposed to some fresh plant foods has been called pollen food allergy syndrome (PFAS). In the wake of PFAS, several different associations of allergenic sources have been progressively proposed and called syndromes. Molecular allergology has shown that these associations are based on IgE co-recognition taking place between homologous allergens present in different allergenic sources. In addition, the molecular approach reveals that some allergens involved in OAS are also responsible for systemic reactions, as in the case of some food Bet v 1-related proteins, lipid transfer proteins and gibberellin regulated proteins. Therefore, in the presence of a convincing history of OAS, it becomes crucial to perform a patient’s tailored molecule-based diagnosis in order to identify the individual IgE sensitization profile. This information allows the prediction of possible cross-reactions with homologous molecules contained in other sources. In addition, it allows the assessment of the risk of developing more severe symptoms on the basis of the features of the allergenic proteins to which the patient is sensitized. In this context, we aimed to provide an overview of the features of relevant plant allergenic molecules and their involvement in the clinical onset of OAS. The value of a personalized molecule-based approach to OAS diagnosis is also analyzed and discussed.

وصف الملف: electronic resource

العلاقة: http://link.springer.com/article/10.1186/s13601-020-00329-8Test; https://doaj.org/toc/2045-7022Test

الوصول الحر: https://doaj.org/article/1cfff5607c8a4a3fb8f8a4cc9da56cffTest

المؤلفون: Yulia Dorofeeva, Paolo Colombo, Miguel Blanca, Adriano Mari, Roman Khanferyan, Rudolf Valenta, Margarete Focke-Tejkl

المصدر: Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019)

مصطلحات موضوعية: Medicine, Science

الوصف: Abstract The weed wall pellitory, Parietaria judaica, is one the most important pollen allergen sources in the Mediterranean area causing severe symptoms of hay fever and asthma in allergic patients. We report the expression of the major Parietaria allergens, Par j 1 and Par j 2 which belong to the family of lipid transfer proteins, in insect cells. According to circular dichroism analysis and gel filtration, the purified allergens represented folded and monomeric proteins. Insect cell-expressed, folded Par j 2 exhibited higher IgE binding capacity and more than 100-fold higher allergenic activity than unfolded Escherichia coli-expressed Par j 2 as demonstrated by IgE ELISA and basophil activation testing. IgE ELISA inhibition assays showed that Par j 1 and Par j 2, contain genuine and cross-reactive IgE epitopes. IgG antibodies induced by immunization with Par j 2 inhibited binding of allergic patients IgE to Par j 1 only partially. IgE inhibition experiments demonstrated that insect cell-expressed Par j 1 and Par j 2 together resembled the majority of allergenic epitopes of the Parietaria allergome and therefore both should be used for molecular diagnosis and the design of vaccines for allergen-specific immunotherapy of Parietaria allergy.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2045-2322Test

الوصول الحر: https://doaj.org/article/a736b1f6001d4b09a56aa18c1c6b4494Test

المؤلفون: Lisa Tuppo, Claudia Alessandri, Ivana Giangrieco, Maurizio Tamburrini, Ricardo Hernandez Arriaza, Maksymilian Chruszcz, Adriano Mari, Maria Antonietta Ciardiello

المصدر: Frontiers in Allergy, Vol 2 (2021)

مصطلحات موضوعية: apple GRP, applemaclein, food allergy, Pru p 7, IgE-binding, sensitization frequency, Immunologic diseases. Allergy, RC581-607

الوصف: Background: The literature reports describing allergic symptoms against apples in the patients sensitized to the gibberellin-regulated proteins (GRPs) suggested the presence of an allergenic GRP in this fruit.Objective: This study aimed to assess the presence of a GRP protein in apples and investigate its allergenicity.Methods: The protein was isolated and identified by the classical biochemical methods. The bioinformatics tools were used for similar searches and molecular modeling. The immunological features were investigated using the multiplex FABER test. Clinical data were collected by the allergy specialists.Results: A GRP was detected in the apple peel and pulp and it was named applemaclein. This protein displays 94% of sequence identity with peamaclein, Pru p 7, representing the prototype of this allergen family. The applemaclein molecular model shows a very irregular surface with grooves/clefts that may potentially accommodate small molecular ligands. In a population of 4,721 patients in Italy, 187 (4.0%) were sensitized to any allergenic GPR. Of those, 115 (61.5%), 61 (32.6%), 30 (16.0%), and 99 (52.9%) had immunoglobulin E (IgE) to apple, peach, pomegranate, and cypress GRP, respectively. However, in a cohort of the patients in Italy, most individuals IgE positive to the apple GRP did not report allergic reactions against this fruit.Conclusion: Compared with the peach Pru p 7, applemaclein shows some different structural features and higher sensitization frequency, which is often not associated with allergic reactions against apple. Further studies are needed to understand a possible correlation between the applemaclein structural properties, the interaction with still unknown molecules, and immunological behavior.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/falgy.2021.745825/fullTest; https://doaj.org/toc/2673-6101Test

الوصول الحر: https://doaj.org/article/6c5f1ef6e46649c896e4da391c8a4008Test

المؤلفون: Lisa Tuppo, Ivana Giangrieco, Maurizio Tamburrini, Claudia Alessandri, Adriano Mari, Maria Antonietta Ciardiello

المصدر: Foods, Vol 11, Iss 6, p 878 (2022)

مصطلحات موضوعية: food allergens, IgE binding, IgE-binding inhibition, ISAC test, FABER test, multiplex allergen microarray, Chemical technology, TP1-1185

الوصف: Several factors can affect the allergen content and profile of a specific food, including processing procedures often leading to a decrease in allergenicity, although no change, or even an increase, have also been reported. Evaluation of the effectiveness of a processing procedure requires the availability of reliable methodologies to assess the variation in molecules able to induce allergic reactions in the analyzed food. Conventional and innovative strategies and methodologies can be exploited to identify allergenic proteins in foodstuffs. However, depending on the specific purposes, different methods can be used. In this review, we have critically reviewed the advantages of an innovative method, the multiplex allergen microarray-based immunoassay, in the detection of allergens in foodstuffs. In particular, we have analyzed some studies reporting the exploitation of an IgE-binding inhibition assay on multiplex allergen biochips, which has not yet been reviewed in the available literature. Unlike the others, this methodology enables the identification of many allergenic proteins, some of which are still unknown, which are recognized by IgE from allergic patients, with a single test. The examined literature suggests that the inhibition test associated with the multiplex allergen immunoassay is a promising methodology exploitable for the detection of IgE-binding proteins in food samples.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2304-8158/11/6/878Test; https://doaj.org/toc/2304-8158Test

الوصول الحر: https://doaj.org/article/700dc7f492d140b2abc0a5c2a0f06a79Test

المؤلفون: Maria C D’Ovidio, Annarita Wirz, Danila Zennaro, Stefania Massari, Paola Melis, Vittoria M Peri, Chiara Rafaiani, Maria C Riviello, Adriano Mari

المصدر: AIMS Public Health, Vol 5, Iss 4, Pp 352-365 (2018)

مصطلحات موضوعية: occupational allergy, laboratory animals, protein microarray, questionnaire, health workers, Public aspects of medicine, RA1-1270

الوصف: Background: Laboratory Animal Allergy (LAA) has been considered a risk for the workers since 1989 by the NIOSH. About one third of the Laboratory Animal Workers (LAWs) can manifest symptoms to LAA as asthma, rhinitis, conjunctivitis and cutaneous reactions. The prevalence of LAA-induced clinical symptoms has been estimated with a great variability (4–44%) also due to the different methodologies applied. Objective: Evaluate the prevalence of IgE positivity to mouse and rat allergens in LAWs and assess which factors are predisposing to sensitization among subjects exposed to laboratory animals in the workplace. Methods: One hundred LAWs were invited to fill out a questionnaire regarding current allergic symptoms, atopic history, home environment, previous and current occupational history. IgE reactivity versus specific allergens was evaluated with ImmunoCAP ISAC. Results: Out of one hundred LAWs, 18% had a serum susceptibility to mouse and/or rat allergens and 42% reported to have occupational allergy symptoms. Combining the results acquired by ImmunoCAP ISAC and questionnaire, 17% of LAWs have been defined as LAWs-LAA positive since they present a positive IgE response and allergy symptoms, 1% LAWs-LAA sensitized, 25% LAWs-LAA symptomatic and 57% LAWs-LAA negative. Presence of previous allergy symptoms in work and life environment were significantly related to LAWs-LAA positive/sensitized. Conclusions: The study aimed to define the immunological profile of LAWs using the proteomic array as an innovative approach in the study of environmental and occupational exposure to allergens. We suggested a definition of LAWs-LAA considering serum IgE response and presence of allergy symptoms. The proposed approach has the advantage to provide a standard methodology for evaluating the specific IgE responsiveness to animal allergens in specific workplace also considering the immunological profile of workers referred to exposure in life and occupational environment.

وصف الملف: electronic resource

العلاقة: http://www.aimspress.com/article/10.3934/publichealth.2018.4.352/fulltext.htmlTest; https://doaj.org/toc/2327-8994Test

الوصول الحر: https://doaj.org/article/8ecae126a78c431d8d51b5b8396ad2f4Test

المؤلفون: Mattia Santoni, Maria Antonietta Ciardiello, Roberta Zampieri, Mario Pezzotti, Ivana Giangrieco, Chiara Rafaiani, Michela Ciancamerla, Adriano Mari, Linda Avesani

المصدر: Frontiers in Plant Science, Vol 10 (2019)

مصطلحات موضوعية: allergen, molecular farming, transient expression, IgE, structure homology, Plant culture, SB1-1110

الوصف: Allergic disease diagnosis is currently experiencing a breakthrough due to the use of allergenic molecules in serum-based assays rather than allergen extracts in skin tests. The former methodology is considered a very innovative technology compared with the latter, since it is characterized by flexibility and adaptability to the patient’s clinical history and to microtechnology, allowing multiplex analysis. Molecular-based analysis requires pure allergens to detect IgE sensitization, and a major goal, to maintain the diagnosis cost-effective, is to limit their production costs. In addition, for the production of recombinant eukaryotic proteins similar to natural ones, plant-based protein production is preferred to bacterial-based systems due to its ability to perform most of the post-translational modifications of eukaryotic molecules. In this framework, Plant Molecular Farming (PMF) may be useful, being a production platform able to produce complex recombinant proteins in short time-frames at low cost. As a proof of concept, PMF has been exploited for the production of Bet v 1a, a major allergen associated with birch (Betula verrucosa) pollen allergy. Bet v 1a has been produced using two different transient expression systems in Nicotiana benthamiana plants, purified and used in a new generation multiplex allergy diagnosis system, the patient-Friendly Allergen nano-BEad Array (FABER). Plant-made Bet v 1a is immunoreactive, binding IgE and inhibiting IgE-binding to the Escherichia coli expressed allergen currently available in the FABER test, thus suggesting an overall similar though non-overlapping immune activity compared with the E. coli expressed form.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/article/10.3389/fpls.2019.01273/fullTest; https://doaj.org/toc/1664-462XTest

الوصول الحر: https://doaj.org/article/ea4ec2437d1c46ce94256df8fde665cbTest