المؤلفون: Mallagaray, Álvaro, Rudolph, Lorena, Lindloge, Melissa, Mölbitz, Jarne, Thomsen, Henrik, Schmelter, Franziska, Alhabash, Mohamad Ward, Abdullah, Mohammed R., Saraei, Roza, Ehlers, Marc, Graf, Tobias, Sina, Christian, Petersmann, Astrid, Nauck, Matthias A., Günther, Ulrich L.

مصطلحات موضوعية: Acute Phase Proteins, Diagnostic Tool, GlycA/B, Glycoproteins, NMR, NMR Blood Analyses, NMR Metabolomics

الوصف: Nuclear Magnetic Resonance (NMR) spectra of human serum and plasma show, besides metabolites and lipoproteins, two characteristic signals termed GlycA and B arising from the acetyl groups of glycoprotein glycans from acute phase proteins, which constitute good markers for inflammatory processes. Here, we report a comprehensive assignment of glycoprotein glycan NMR signals observed in human serum, showing that GlycA and GlycB signals originate from Neu5Ac and GlcNAc moieties from N-glycans, respectively. Diffusion-edited NMR experiments demonstrate that signal components can be associated with specific acute phase proteins. Conventionally determined concentrations of acute phase glycoproteins correlate well with distinct features in NMR spectra (R2 up to 0.9422, p-value <0.001), allowing the simultaneous quantification of several acute phase inflammation proteins. Overall, a proteo-metabolomics NMR signature of significant diagnostic potential is obtained within 10–20 min acquisition time. This is exemplified in serum samples from COVID-19 and cardiogenic shock patients showing significant changes in several acute phase proteins compared to healthy controls. ; 62 ; 35

العلاقة: Angewandte Chemie. International edition; https://publica.fraunhofer.de/handle/publica/450976Test

الإتاحة: https://doi.org/10.1002/anie.202306154Test
https://publica.fraunhofer.de/handle/publica/450976Test

المؤلفون: Alcorlo, Martín¹ (AUTHOR), Abdullah, Mohammed R.² (AUTHOR), Steil, Leif³ (AUTHOR), Sotomayor, Francisco¹ (AUTHOR), López-de Oro, Laura¹ (AUTHOR), de Castro, Sonia⁴ (AUTHOR), Velázquez, Sonsoles⁴ (AUTHOR), Kohler, Thomas P.² (AUTHOR), Jiménez, Elisabet⁵ (AUTHOR), Medina, Ana⁵ (AUTHOR), Usón, Isabel^5,6 (AUTHOR), Keller, Lance E.^7,8 (AUTHOR), Bradshaw, Jessica L.⁹ (AUTHOR), McDaniel, Larry S.^7,8 (AUTHOR), Camarasa, María-José⁴ (AUTHOR), Völker, Uwe³ (AUTHOR), Hammerschmidt, Sven² (AUTHOR) sven.hammerschmidt@uni-greifswald.de, Hermoso, Juan A.¹ (AUTHOR) sven.hammerschmidt@uni-greifswald.de

المصدر: PLoS Pathogens. 6/5/2024, Vol. 20 Issue 6, p1-30. 30p.

مصطلحات موضوعية: *STREPTOCOCCUS pneumoniae, *BIOLOGICAL transport, *ATP-binding cassette transporters, *PEPTIDES, *CARRIER proteins, *ODORANT-binding proteins

مستخلص: ATP-binding cassette (ABC) transport systems are crucial for bacteria to ensure sufficient uptake of nutrients that are not produced de novo or improve the energy balance. The cell surface of the pathobiont Streptococcus pneumoniae (pneumococcus) is decorated with a substantial array of ABC transporters, critically influencing nasopharyngeal colonization and invasive infections. Given the auxotrophic nature of pneumococci for certain amino acids, the Ami ABC transporter system, orchestrating oligopeptide uptake, becomes indispensable in host compartments lacking amino acids. The system comprises five exposed Oligopeptide Binding Proteins (OBPs) and four proteins building the ABC transporter channel. Here, we present a structural analysis of all the OBPs in this system. Multiple crystallographic structures, capturing both open and closed conformations along with complexes involving chemically synthesized peptides, have been solved at high resolution providing insights into the molecular basis of their diverse peptide specificities. Mass spectrometry analysis of oligopeptides demonstrates the unexpected remarkable promiscuity of some of these proteins when expressed in Escherichia coli, displaying affinity for a wide range of peptides. Finally, a model is proposed for the complete Ami transport system in complex with its various OBPs. We further disclosed, through in silico modelling, some essential structural changes facilitating oligopeptide transport into the cellular cytoplasm. Thus, the structural analysis of the Ami system provides valuable insights into the mechanism and specificity of oligopeptide binding by the different OBPs, shedding light on the intricacies of the uptake mechanism and the in vivo implications for this human pathogen. Author summary: The uptake of diverse oligopeptides enables pneumococcal growth despite auxotrophies and functions as a critical sensor for assessing the composition of the local environment. The identification of additional OBPs in non-encapsulated S. pneumoniae strains suggests their involvement in sensing a broader spectrum of bacterial competitors coexisting with the highly commensal pneumococcus. This study presents a comprehensive analysis of the initial phase of peptide transport mediated by OBPs within the pneumococcal Ami permease system. We disclose a common mechanism for oligopeptide recognition that is modulated in each OBP to accommodate a diverse array of oligopeptides. Understanding how pneumococcus perceives external stimuli and responds to them is imperative for unraveling the transition from a commensal to a pathogenic state. [ABSTRACT FROM AUTHOR]

المؤلفون: Mills, Richael O., Abdullah, Mohammed R., Akwetey, Samuel A., Sappor, Dorcas C., Bolivar, Johan A., Gámez, Gustavo, van der Linden, Mark P. G., Hammerschmidt, Sven

الوصف: Preventive strategies involving the use of pneumococcal conjugate vaccines (PCVs) are known to drastically reduce pneumococcal disease. However, PCV vaccination has been plagued with serotype replacement by non-PCV serotypes. In this study, we describe the prevalence and molecular characteristics of non-PCV13 serotypes (non-vaccine serotypes, NVTs) from pneumococcal carriage isolates obtained from children < 5 years old in Cape Coast, Ghana, after PCV introduction. The isolates were subjected to antibiotic susceptibility testing and multilocus sequence typing (MLST), and molecular techniques were used to detect the presence of virulence genes. Serotypes 11A, 13, 15B, 23B, and 34 formed the top five of the 93 NVT isolates. As such, 20 (21.5%), 49 (48.4%), and 70 (74.3%) isolates were non-susceptible to penicillin, tetracycline, and cotrimoxazole, respectively. Sixteen (17.2%) multidrug-resistant isolates were identified. However, non-susceptibility to ceftriaxone and erythromycin was low and all isolates were fully susceptible to levofloxacin, linezolid, and vancomycin. Whereas pcpA, pavB, lytA, and psrP genes were detected in nearly all serotypes, pilus islet genes were limited to serotypes 11A, 13, and 23B. MLST for predominant serotype 23B isolates revealed three known and seven novel sequence types (STs). ST172 and novel ST15111 were the most dominant and both STs were related to PMEN clone Columbia23F-26 (ST338). In conclusion, non-PCV13 serotype 23B was the most prevalent, with characteristics of rapid clonal expansion of ST172 and ST15111, which are related to international clones of the pneumococcus. Continuous monitoring of NVTs in Ghana is, therefore, essential, as they have the potential to cause invasive disease, show high antibiotic resistance, and attenuate the effects of PCV vaccination.

وصف الملف: application/pdf

العلاقة: https://epub.ub.uni-greifswald.de/frontdoor/index/index/docId/7612Test; urn:nbn:de:gbv:9-opus-76127; https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-76127Test; https://doi.org/10.3390/microorganisms10102054Test; https://epub.ub.uni-greifswald.de/files/7612/microorganisms-10-02054-v2.pdfTest

الإتاحة: https://doi.org/10.3390/microorganisms10102054Test
https://epub.ub.uni-greifswald.de/frontdoor/index/index/docId/7612Test
https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-76127Test
https://epub.ub.uni-greifswald.de/files/7612/microorganisms-10-02054-v2.pdfTest

المؤلفون: Mills, Richael O., Abdullah, Mohammed R., Akwetey, Samuel A., Sappor, Dorcas C., Gámez, Gustavo, Hammerschmidt, Sven

الوصف: Antibiotic resistance in pneumococci contributes to the high pneumococcal deaths in children. We assessed the molecular characteristics of multidrug-resistant (MDR) pneumococci isolated from healthy vaccinated children under five years of age in Cape Coast, Ghana. A total of 43 MDR isolates were selected from 151 pneumococcal strains obtained from nasopharyngeal carriage. All isolates were previously serotyped by multiplex PCR and Quellung reaction. Susceptibility testing was performed using either the E-test or disk diffusion method. Virulence and antibiotic resistance genes were identified by PCR. Molecular epidemiology was analyzed using multilocus sequence typing (MLST). Vaccine-serotypes 23F and 19F were predominant. The lytA and pavB virulence genes were present in all isolates, whiles 14–86% of the isolates carried pilus-islets 1 and 2, pcpA, and psrP genes. Penicillin, tetracycline, and cotrimoxazole resistance were evident in >90% of the isolates. The ermB, mefA, and tetM genes were detected in (n = 7, 16.3%), (n = 4, 9.3%) and (n = 43, 100%) of the isolates, respectively. However, >60% showed alteration in the pbp2b gene. MLST revealed five novel and six known sequence types (STs). ST156 (Spain9V-3) and ST802 were identified as international antibiotic-resistant clones. The emergence of international-MDR clones in Ghana requires continuous monitoring of the pneumococcus through a robust surveillance system.

وصف الملف: application/pdf

العلاقة: https://epub.ub.uni-greifswald.de/frontdoor/index/index/docId/6080Test; urn:nbn:de:gbv:9-opus-60804; https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-60804Test; https://doi.org/10.3390/microorganisms10020469Test; https://epub.ub.uni-greifswald.de/files/6080/microorganisms-10-00469-v2.pdfTest

الإتاحة: https://doi.org/10.3390/microorganisms10020469Test
https://epub.ub.uni-greifswald.de/frontdoor/index/index/docId/6080Test
https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-60804Test
https://epub.ub.uni-greifswald.de/files/6080/microorganisms-10-00469-v2.pdfTest

المؤلفون: Hannich, Malte Johannes, Abdullah, Mohammed R., Budde, Kathrin, Petersmann, Astrid, Nauck, Matthias, Dressel, Alexander, Süße, Marie

الوصف: We performed this cohort study to test whether further analysis of intrathecal inflammation can be omitted if the free light chain kappa (FLCκ) quotient is within the reference range in the corresponding quotient diagram. FLCκ concentrations were measured in serum and cerebrospinal fluid (CSF) samples. The intrathecal fraction (IF) of FLCκ was calculated in relation to the hyperbolic reference range. 679 patient samples were used as a discovery cohort (DC). The sensitivity and negative predictive value (NPV) of the FLCκ-IF for the detection of an intrathecal humoral immune response (CSF-specific OCB and/or IF IgG/A/M > 0%) was determined. Based on these data, a diagnostic algorithm was developed and prospectively validated in an independent validation cohort (VC, n = 278). The sensitivity of the FLCκ-IF was 98% in the DC and 97% in the VC with a corresponding NPV of 99%. The use of the FLCκ-IF as a first line analysis would have reduced the Ig and OCB analysis by 62% in the DC and 74% in the VC. The absence of a FLCκ-IF predicts the absence of a humoral intrathecal immune response with a very high NPV of 99%. Thus, integration of our proposed algorithm into routine CSF laboratory analysis could help to reduce analytical efforts.

وصف الملف: application/pdf

العلاقة: https://epub.ub.uni-greifswald.de/frontdoor/index/index/docId/9446Test; urn:nbn:de:gbv:9-opus-94463; https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-94463Test; https://doi.org/10.3390/biom12111690Test; https://epub.ub.uni-greifswald.de/files/9446/biomolecules-12-01690-v2.pdfTest

الإتاحة: https://doi.org/10.3390/biom12111690Test
https://epub.ub.uni-greifswald.de/frontdoor/index/index/docId/9446Test
https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-94463Test
https://epub.ub.uni-greifswald.de/files/9446/biomolecules-12-01690-v2.pdfTest

المؤلفون: Saeed, Tahseen S., Albalawi, Sarah S., Abuhagr, Abubkr, Althobaiti, Saja Abdulrahman, Hassanain, Hawazen M., Reeves, Donald M., Abdullah, Mohammed R., Sinn, Ekkehard

المصدر: New Journal of Chemistry; 12/21/2023, Vol. 47 Issue 47, p21661-21669, 9p

مصطلحات موضوعية: FLAVIN mononucleotide, TITANIUM dioxide, TITANIUM dioxide nanoparticles, TITANIUM dioxide surfaces, CHARGE exchange, PERFLUOROOCTANOIC acid

مستخلص: Flavin mononucleotide (FMN) in methanol was photochemically reduced to FMNH2, which undergoes electron transfer with Methyl viologen (MV²⁺) in ethanol. This charge transfer interaction results in the reduction of MV²⁺ into MV⁺˙, as well as the storage of electrons. The spectroscopic experiments allow for the elucidation of quantitative electron transfer into MV²⁺. To study the redox potential for FMN species, the HOMO and LUMO energies were calculated using density functional theory (DFT) method on the B3LYP level with a basis set of 6-311G(d,p). The HOMO and LUMO energy calculations show that the redox state of FMNH2 is energetically more favorable than FMNH˙ for MV²⁺ reduction. These studies revealed good agreement between the experimental results and computational predictions. Moreover, molecular electrostatic potential (MEP) was performed on the FMNH2-MV²⁺ complex to determine the site of electron transfer, and the results were compared to the experimental results. FMN functionalized to the surface of colloidal titanium dioxide nanoparticles (TiO2 NPs) showed enhanced reactivity toward the reduction of perfluorooctanoic acid (PFOA). The two-electron transfer reduction process is generated by irradiating FMN attached to TiO2, during which FMN reduces to FMNH2. The FMNH2 then reacts with PFOA to produce shorter chain structures with fewer fluorine atoms, such as C5F11COOH. The results indicate that the rational organization of FMNH2/TiO2 to mediate two-electron transfer is effective for the reductive degradation and remediation of PFOA. [ABSTRACT FROM AUTHOR]

: Copyright of New Journal of Chemistry is the property of Royal Society of Chemistry and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Seinen, Jolien, Engelke, Rudolf, Abdullah, Mohammed R, Voß, Franziska, Michalik, Stephan, Dhople, Vishnu M, Dieperink, Willem, de Smet, Anne Marie G A, Völker, Uwe, van Dijl, Jan Maarten, Schmidt, Frank, Hammerschmidt, Sven

المصدر: Seinen , J , Engelke , R , Abdullah , M R , Voß , F , Michalik , S , Dhople , V M , Dieperink , W , de Smet , A M G A , Völker , U , van Dijl , J M , Schmidt , F & Hammerschmidt , S 2021 , ' Sputum Proteome Signatures of Mechanically Ventilated Intensive Care Unit Patients Distinguish Samples with or without Anti-pneumococcal Activity ' , Msystems , vol. 6 , no. 2 , e00702-20 . https://doi.org/10.1128/mSystems.00702-20Test

مصطلحات موضوعية: Streptococcus pneumoniae, mechanical ventilation, sputum, antimicrobial peptides, immunoproteomics, proteomics

الوصف: Mechanically ventilated patients are at risk of contracting pneumonia. Therefore, these patients often receive prophylactic systemic antimicrobial therapy. Intriguingly however, a previous study showed that antimicrobial activity in bronchoalveolar aspirates (here referred to as "sputa") from ventilated patients was only partially explained by antibiotic therapy. Here we report that sputa from these patients presented distinct proteome signatures depending on the presence or absence of antimicrobial activity. Moreover, we show that the same distinction applied to antibodies against Streptococcus pneumoniae, which is a major causative agent of pneumonia. Specifically, the investigated sputa that inhibited growth of S. pneumoniae, while containing subinhibitory levels of the antibiotic cefotaxime, presented elevated levels of proteins implicated in innate immune defenses, including complement and apolipoprotein-associated proteins. In contrast, S. pneumoniae-inhibiting sputa with relatively high cefotaxime concentrations or noninhibiting sputa contained higher levels of proteins involved in inflammatory responses, such as neutrophil elastase-associated proteins. In an immunoproteomics analysis, 18 out of 55 S. pneumoniae antigens tested showed significantly increased levels of IgGs in inhibiting sputa. Hence, proteomics and immunoproteomics revealed elevated levels of antimicrobial host proteins or S. pneumoniae antigen-specific IgGs in pneumococcal growth-inhibiting sputa, thus explaining their anti-pneumococcal activity. IMPORTANCE Respiratory pathogens like Streptococcus pneumoniae can cause severe pneumonia. Nonetheless, mechanically ventilated intensive care patients, who have a high risk of contracting pneumonia, rarely develop pneumococcal pneumonia. This suggests the presence of potentially protective antimicrobial agents in their lung environment. Our present study shows for the first time that bronchoalveolar aspirates, "sputa," of ventilated patients in a Dutch intensive care unit were characterized by ...

وصف الملف: application/pdf

العلاقة: https://research.rug.nl/en/publications/ea91fd4e-e82d-49e6-9ff5-70a8a61fc9a5Test

الإتاحة: https://doi.org/10.1128/mSystems.00702-20Test
https://hdl.handle.net/11370/ea91fd4e-e82d-49e6-9ff5-70a8a61fc9a5Test
https://research.rug.nl/en/publications/ea91fd4e-e82d-49e6-9ff5-70a8a61fc9a5Test
https://pure.rug.nl/ws/files/163988654/Sputum_Proteome_Signatures_of_Mechanically_Ventilated_Intensive_Care_Unit_Patients_Distinguish_Samples_with_or_without_Anti_pneumococcal_Activity.pdfTest

المؤلفون: He, Samantha W. J., van de Garde, Martijn D. B., Pieren, Daan K. J., Poelen, Martien C. M., Voß, Franziska, Abdullah, Mohammed R., Hammerschmidt, Sven, van Els, Cécile A. C. M.

الوصف: Respiratory infection caused by Streptococcus pneumoniae is a leading cause of morbidity and mortality in older adults. Acquired CD4+ T cell mechanism are essential for the protection against colonization and subsequent development of infections by S. pneumoniae. In this study, we hypothesized that age-related changes within the CD4+ T-cell population compromise CD4+ T-cell specific responses to S. pneumoniae, thereby contributing to increased susceptibility at older age. To this end, we interrogated the CD4+ T-cell response against the immunogenic pneumococcal protein AliB, part of the unique oligopeptide ABC transporter system responsible for the uptake of nutrients for the bacterium and crucial for the development of pneumococcal meningitis, in healthy young and older adults. Specifically, proliferation of CD4+ T cells as well as concomitant cytokine profiles and phenotypic markers implied in immunosenescence were studied. Older adults showed decreased AliB-induced CD4+ T-cell proliferation that is associated with an increased frequency of regulatory T cells and lower levels of active CD25+CD127+CTLA-4−TIGIT-CD4+T cells. Additionally, levels of pro-inflammatory cytokines IFNy and IL-17F were decreased at older age. Our findings indicate that key features of a pneumococcal-specific CD4+ T-cell immune response are altered at older age, which may contribute to enhanced susceptibility for pneumococcal infections.

وصف الملف: application/pdf

العلاقة: https://epub.ub.uni-greifswald.de/frontdoor/index/index/docId/6471Test; urn:nbn:de:gbv:9-opus-64719; https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-64719Test; https://doi.org/10.3389/fragi.2021.746295Test; https://epub.ub.uni-greifswald.de/files/6471/fragi-02-746295.pdfTest

الإتاحة: https://doi.org/10.3389/fragi.2021.746295Test
https://epub.ub.uni-greifswald.de/frontdoor/index/index/docId/6471Test
https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-64719Test
https://epub.ub.uni-greifswald.de/files/6471/fragi-02-746295.pdfTest

المؤلفون: Mills, Richael O., Abdullah, Mohammed R., Akwetey, Samuel A., Sappor, Dorcas C., Cole, Isaac, Baffuor-Asare, Michael, Bolivar, Johan A., Gámez, Gustavo, van der Linden, Mark P. G., Hammerschmidt, Sven

وصف الملف: application/pdf

العلاقة: https://epub.ub.uni-greifswald.de/frontdoor/index/index/docId/4257Test; urn:nbn:de:gbv:9-opus-42578; https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-42578Test; https://doi.org/10.3390/microorganisms8121987Test; https://epub.ub.uni-greifswald.de/files/4257/microorganisms-08-01987.pdfTest

الإتاحة: https://doi.org/10.3390/microorganisms8121987Test
https://epub.ub.uni-greifswald.de/frontdoor/index/index/docId/4257Test
https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-42578Test
https://epub.ub.uni-greifswald.de/files/4257/microorganisms-08-01987.pdfTest

المؤلفون: Abdullah, Mohammed R., Ibrahim, Abdullahi Abdu, Wasel, Noorulden B. Mohamad, Abdulwahid, Maan M.

المساهمون: Noorulden B Mohamad, Ibrahim, Abdullahi Abdu

مصطلحات موضوعية: BOA, FOPID, PID, Steering Control

الوصف: 2-s2.0-85090290888 ; Recently several optimization methods have been involves with the enhancement of the control system. One of the most interesting method was based on the use of the Bees Optimization Algorithm (BOA) in controlled system. In this work it has been use it as an enhancement method for controlling the steering control system for electric golf cart, which could increment the progression high stability in voltage and decrease the Steady-state error, high overshoot, inertness for rising time and settling time these issues that we look to unravel it in the controlled system to get limit of dependability that makes controller work in a decent case. The advanced calculations will be utilized from BOA calculation and demonstrate that the utilization with Optimized calculations is superior to the use of the Fractional-order Proportional Integral Derivative (FOPID) controller when making tuning. The applied of the controlled system (Steering Control System for Electric Golf Cart) SCS-EGC system by utilizing MATLAB Simulink. © 2020, World Academy of Research in Science and Engineering. All rights reserved.

وصف الملف: application/pdf

العلاقة: International Journal of Advanced Trends in Computer Science and Engineering; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; https://doi.org/10.30534/ijatcse/2020/56942020Test; https://hdl.handle.net/20.500.12939/1089Test; 4575; 4579

الإتاحة: https://doi.org/20.500.12939/1089Test
https://doi.org/10.30534/ijatcse/2020/56942020Test
https://hdl.handle.net/20.500.12939/1089Test