المؤلفون: Olmez N., Koc T., Semiz A., Lale Satiroglu-Tufan N., Celik-Turgut G., Dodurga Y., Sen, Alaattin, Ozgun-Acar O.

مصطلحات موضوعية: Aryl hydrocarbon receptor, Cytochrome P4501A1, Hepatitis B virus X protein, Hepatocellular carcinoma, Nuclear factor-kappa B, aromatic hydrocarbon receptor, cytochrome P450 1A1, firefly luciferase, immunoglobulin enhancer binding protein, Renilla luciferin 2 monooxygenase, AHR gene, Article, carcinogenicity, cell viability, coimmunoprecipitation, controlled study, CYP1A1 gene, gene expression, gene interaction, Hep-G2 cell line, human, human cell, inflammation, liver cell carcinoma, luciferase assay, molecular interaction, NF kB gene, NF kB signaling, protein expression, protein protein interaction

الوصف: In this study, it was aimed to elucidate the interaction between aryl hydrocarbon receptor (AHR), nuclear factor-kappa B (NF-kB), and cytochrome P4501A1 (CYP1A1) with hepatitis B virus X protein (HBX) in a human liver cancer cell line (HepG2) transfected with HBX. First, AHR, NF-kB, and CYP1A1 genes were cloned into the appropriate region of the CheckMate mammalian two-hybrid recipient plasmids using a flexi vector system. Renilla and firefly luciferases were quantified using the dual-luciferase reporter assay system to measure the interactions. Secondly, transient transfections of CYP1A1 and NF-kB (RelA) were performed into HBX–positive and HBX–negative HepG2 cells. The mRNA expression of CYP1A1 and NF-kB genes were confirmed with RT-PCR, and cell viability was measured by WST-1. Further verification was assessed by measuring the activity and protein level of CYP1A1. Additionally, CYP1A1/HBX protein–protein interactions were performed with co-immunoprecipitation, which demonstrated no interaction. These results have clearly shown that the NF-kB and AHR genes interact with HBX without involving CYP1A1 and HBX protein–protein interactions. The present study confirms that AHR and NF-kB interaction plays a role in the HBV mechanism mediated via HBX and coordinating the carcinogenic or inflammatory responses; still, the CYP1A1 gene has no effect on this interaction. © 2022 Elsevier B.V. ; 2011FBE053, 2014FBE028; Türkiye Bilimsel ve Teknolojik AraÅŸtırma Kurumu, TÃœBÄ°TAK: 111T612 ; This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) [Grant No 111T612 ]; and Pamukkale University Department of Scientific Research Projects (PAU-BAP) [grant number 2011FBE053 and 2014FBE028].

العلاقة: Gene; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; https://doi.org/10.1016/jTest.gene.2022.147099; https://hdl.handle.net/11499/47438Test; 853; 2-s2.0-85143722253; WOS:000904587300001

الإتاحة: https://doi.org/10.1016/jTest.gene.2022.147099
https://hdl.handle.net/11499/47438Test

المؤلفون: Smirnov, Vasily M, Lasseaux, Eulalie, Michaud, Vincent, Courdier, Cécile, Meunier, Isabelle, Arveiler, Benoit, Defoort-Dhellemmes, Sabine

المصدر: Doc Ophthalmol ; ISSN:1573-2622

مصطلحات موضوعية: AHR gene, Crossed VEP asymmetry, Foveal hypoplasia, Infantile nystagmus

الوصف: Infantile nystagmus and foveal hypoplasia associated with AHR gene defects is a newly recognized and rare disorder. Our aim was to present a patient with a novel biallelic AHR pathogenic variant with electrophysiological evidence of chiasmal misrouting.

العلاقة: https://doi.org/10.1007/s10633-024-09979-6Test; https://pubmed.ncbi.nlm.nih.gov/38922562Test

الإتاحة: https://doi.org/10.1007/s10633-024-09979-6Test
https://pubmed.ncbi.nlm.nih.gov/38922562Test

المؤلفون: Zheng-Ju Ren, DeHong Cao, Peng-Wei Ren, Bo Yang, Dong-Liang Lu, Jian Liao, Sheng-Zhuo Liu, Lu-Cheng Yang, Zhu-Feng Peng, Liang-Ren Liu, Qiang Dong

المصدر: Renal Failure, Vol 41, Iss 1, Pp 987-994 (2019)

مصطلحات موضوعية: aryl hydrocarbon receptor, ahr gene, polymorphism, male infertility, meta-analysis, Diseases of the genitourinary system. Urology, RC870-923

الوصف: To evaluate the relationship between the aryl hydrocarbon receptor (AHR) rs2066853 gene polymorphism and the risk of male infertility. PubMed, Embase, Web of Science, and Chinese National Knowledge Infrastructure (CNKI) were searched for relevant case–control studies up to 31 July 2019. Odds ratio (OR) and 95% confidence interval (95% CI) were used to assess the strength of associations. Finally, seven case–control studies involving 1247 cases and 1762 controls were included in this meta-analysis. The pooled results showed that there was no significant association between AHR rs2066853 gene polymorphism and male infertility risk (A vs. G: OR = 1.08, 95% CI = 0.83–1.39; AA vs. GG: OR = 1.16, 95% CI = 0.65–2.04; AA vs. GA + GG: OR = 1.17, 95% CI = 0.66–2.07; AA + GA vs. GG: OR = 0.99, 95% CI = 0.85–1.15). Subgroup analysis by ethnicity showed the same result. However, significant association was found between AHR rs2066853 gene polymorphism and male infertility risk in oligoasthenotspermia (A vs. G: OR = 2.52, 95% CI = 1.72–3.70). In conclusion, our meta-analysis indicated that AHR rs2066853 gene polymorphism might be associated with an increased susceptibility to oligoasthenotspermia.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/0886-022XTest; https://doaj.org/toc/1525-6049Test

الوصول الحر: https://doaj.org/article/7a834dc6f03b404d8a029ec88ed3c825Test

المؤلفون: Hansen, Bjørn Henrik, Nordtug, Trond, Farkas, Julia, Khan, Essa Ahsan, Oteri, Erika, Kvæstad, Bjarne, Faksness, Liv-Guri, Daling, Per Snorre, Arukwe, Augustine

المصدر: 1-12 ; 237 ; Aquatic Toxicology

مصطلحات موضوعية: Cyp1a, AhR gene battery, Embryotoxicity, Heavy fuel oil, Gas oil, Diesel

الوصف: Due to the heavy fuel oil (HFO) ban in Arctic maritime transport and new legislations restricting the sulphur content of fuel oils, new fuel oil types are continuously developed. However, the potential impacts of these new fuel oil types on marine ecosystems during accidental spills are largely unknown. In this study, we studied the toxicity of three marine fuel oils (two marine gas oils with low sulphur contents and a heavy fuel oil) in early life stages of cod (Gadus morhua). Embryos were exposed for 4 days to water-soluble fractions of fuel oils at concentrations ranging from 4.1 - 128.3 µg TPAH/L, followed by recovery in clean seawater until 17 days post fertilization. Exposure to all three fuel oils resulted in developmental toxicity, including severe morphological changes, deformations and cardiotoxicity. To assess underlying molecular mechanisms, we studied fuel oil-mediated activation of aryl hydrocarbon receptor (Ahr) gene battery and genes related to cardiovascular, angiogenesis and osteogenesis pathways. Overall, our results suggest comparable mechanisms of toxicity for the three fuel oils. All fuel oils caused concentration-dependant increases of cyp1a mRNA which paralleled ahrr, but not ahr1b transcript expression. On the angiogenesis and osteogenesis pathways, fuel oils produced concentration-specific transcriptional effects that were either increasing or decreasing, compared to control embryos. Based on the observed toxic responses, toxicity threshold values were estimated for individual endpoints to assess the most sensitive molecular and physiological effects, suggesting that unresolved petrogenic components may be significant contributors to the observed toxicity. ; publishedVersion

وصف الملف: application/pdf

العلاقة: Aquatic Toxicology. 2021, 237 1-12.; urn:issn:0166-445X; https://hdl.handle.net/11250/3025602Test; https://doi.org/10.1016/j.aquatox.2021.105881Test; cristin:1921771

الإتاحة: https://doi.org/10.1016/j.aquatox.2021.105881Test
https://hdl.handle.net/11250/3025602Test

المؤلفون: Maier, Andrew, Micka, Jana, Miller, Kevin, Denko, Timothey, Chang, Ching-Yi, Nebert, Daniel W., Puga, Alvaro

المصدر: Environmental Health Perspectives, 1998 Jul 01. 106(7), 421-426.

الوصول الحر: https://www.jstor.org/stable/3434070Test

المؤلفون: Qian Zhang, Ruihua Huang, Xiang Ma, Nengjing Jiang, Wuduo Zhou, Chen Gao, Moran Zhao, Peipei Niu, Zongping Zhang, Qiang Li, Juan Zhou, Pinghua Li

المصدر: Animals; Volume 10; Issue 1; Pages: 11

مصطلحات موضوعية: AHR gene, rs339939442, chinese pigs, western pigs, litter size, association analysis

جغرافية الموضوع: agris

الوصف: Eastern and Southern Chinese pigs have been imported to Western countries to improve economic traits including fertility in Western pig breeds by intensive selecting Chinese advantage genes. It was reported that the selected Asian-derived non-synonymous mutations including rs339939442 (G > T) in the aryl hydrocarbon receptor (AHR) gene could increase litter size in multiple European commercial lines. The objective of this study is to identify whether rs339939442 in the AHR gene is polymorphic and has an influence on the litter size in 10 pig populations including five Chinese indigenous breeds, one cultivated breed, one lean-type breed, two North American lean-type breeds, and one European lean-type breed. We found that rs339939442 had polymorphism in all 10 populations, whereas rs339939442 was associated with litter size only in French Yorkshire (FRA-Y) and Chinese cultivated Suhuai (SH) pigs containing approximately 75% British Yorkshire pigs ancestry. Our results indicated that rs339939442 in the AHR gene was a potential marker to improve litter size in European commercial lines and the pigs containing ancestries of European commercial lines, whereas this locus maybe not a causal mutation affecting the litter size but only in linkage disequilibrium with the causal mutation for litter size.

وصف الملف: application/pdf

العلاقة: Animal Genetics and Genomics; https://dx.doi.org/10.3390/ani10010011Test

الإتاحة: https://doi.org/10.3390/ani10010011Test

المؤلفون: Girolami, F., Badino, P., Spalenza, V., Manzini, L., Renzone, G., Salzano, A. M., Dal Piaz, F., Scaloni, A., Rychen, G., Nebbia, C.

المساهمون: Girolami, F., Badino, P., Spalenza, V., Manzini, L., Renzone, G., Salzano, A.M., Dal Piaz, F., Scaloni, A., Rychen, G., Nebbia, C.*

مصطلحات موضوعية: AhR gene, Biomarker, Cattle, MALDI-TOF-MS serum profiling, Polychlorinated biphenyl, TEQ, Environmental Engineering, Environmental Chemistry, Waste Management and Disposal, Pollution

الوصف: Dioxins and polychlorinated biphenyls (PCBs) are widespread and persistent contaminants. Through a combined gene expression/proteomic-based approach, candidate biomarkers of the exposure to such environmental pollutants in cattle subjected to a real eco-contamination event were identified. Animals were removed from the polluted area and fed a standard ration for 6 months. The decontamination was monitored by evaluating dioxin and PCB levels in pericaudal fat two weeks after the removal from the contaminated area (day 0) and then bimonthly for six months (days 59, 125 and 188). Gene expression measurements demonstrated that CYP1B1 expression was significantly higher in blood lymphocytes collected in contaminated animals (day 0), and decreased over time during decontamination. mRNA levels of interleukin 2 showed an opposite quantitative trend. MALDI-TOF-MS polypeptide profiling of serum samples ascertained a progressive decrease (from day 0 to 188) of serum levels of fibrinogen -chain and serpin A3-7-like fragments, apolipoprotein (APO) C-II and serum amyloid A-4 protein, along with an augmented representation of transthyretin isoforms, as well as APOC-III and APOA-II proteins during decontamination. When differentially represented species were combined with serum antioxidant, acute phase and proinflammatory protein levels already ascertained in the same animals (Cigliano et al., 2016), bioinformatics unveiled an interaction network linking together almost all components. This suggests the occurrence of a complex PCB-responsive mechanism associated with animal contamination/decontamination, including a cohort of protein/polypeptide species involved in blood redox homeostasis, inflammation and lipid transport. All together, these results suggest the use in combination of such biomarkers for identifying PCB-contaminated animals, and for monitoring the restoring of their healthy condition following a decontamination process.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/29852444; info:eu-repo/semantics/altIdentifier/wos/WOS:000438408800004; volume:640-641; firstpage:22; lastpage:30; numberofpages:9; journal:SCIENCE OF THE TOTAL ENVIRONMENT; http://hdl.handle.net/2318/1669392Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85047520817; www.elsevier.com/locate/scitotenv

الإتاحة: https://doi.org/10.1016/j.scitotenv.2018.05.284Test
http://hdl.handle.net/2318/1669392Test

المؤلفون: Gurbet Celik-Turgut, Nazmiye Olmez, Tugba Koc, Ozden Ozgun-Acar, Asli Semiz, Yavuz Dodurga, Naciye Lale Satiroglu-Tufan, Alaattin Sen

المصدر: Gene. 853

مصطلحات موضوعية: liver cell carcinoma, Hepatocellular carcinoma, Hep-G2 cell line, Nuclear factor-kappa B, Article, carcinogenicity, Genetics, controlled study, human, Cytochrome P4501A1, protein expression, Aryl hydrocarbon receptor, cell viability, CYP1A1 gene, Renilla luciferin 2 monooxygenase, luciferase assay, gene interaction, human cell, hepatitis B virus X protein, General Medicine, firefly luciferase, aromatic hydrocarbon receptor, AHR gene, immunoglobulin enhancer binding protein, inflammation, real time polymerase chain reaction, protein protein interaction, molecular interaction, gene expression, NF kB gene, coimmunoprecipitation, cytochrome P450 1A1, NF kB signaling

الوصف: In this study, it was aimed to elucidate the interaction between aryl hydrocarbon receptor (AHR), nuclear factor-kappa B (NF-kB), and cytochrome P4501A1 (CYP1A1) with hepatitis B virus X protein (HBX) in a human liver cancer cell line (HepG2) transfected with HBX. First, AHR, NF-kB, and CYP1A1 genes were cloned into the appropriate region of the CheckMate mammalian two-hybrid recipient plasmids using a flexi vector system. Renilla and firefly luciferases were quantified using the dual-luciferase reporter assay system to measure the interactions. Secondly, transient transfections of CYP1A1 and NF-kB (RelA) were performed into HBX–positive and HBX–negative HepG2 cells. The mRNA expression of CYP1A1 and NF-kB genes were confirmed with RT-PCR, and cell viability was measured by WST-1. Further verification was assessed by measuring the activity and protein level of CYP1A1. Additionally, CYP1A1/HBX protein–protein interactions were performed with co-immunoprecipitation, which demonstrated no interaction. These results have clearly shown that the NF-kB and AHR genes interact with HBX without involving CYP1A1 and HBX protein–protein interactions. The present study confirms that AHR and NF-kB interaction plays a role in the HBV mechanism mediated via HBX and coordinating the carcinogenic or inflammatory responses; still, the CYP1A1 gene has no effect on this interaction. © 2022 Elsevier B.V.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::559b3a1f86e4992196f17d5daf0f7a42Test
https://pubmed.ncbi.nlm.nih.gov/36476661Test

المؤلفون: Aftabi, Younes, Amiri-Sadeghan, Amiri, Gilani, Neda, Bakhtiyari, Nasim, Ansarin, Atefeh, Sharifi, Akbar, Ansarin, Khalil, Khalili, Yeganeh, Seyedrezazadeh, Ensiyeh

المصدر: Indian Journal of Biochemistry and Biophysics (IJBB); IJBB Vol. 59 (3) [March 2022]; 311-319

مصطلحات موضوعية: AhR gene, Lung diseases, Genetic association, rs2066853, Polymorphism

الوصف: Aryl hydrocarbon Receptor (AhR) is a ligand-activated transcription factor with an important role in lung health. The association of AhR polymorphisms with asthma severity has not been yet investigated. We analyzed the association of G1661A, the most prevalent polymorphism of AhR, with the asthma stages in a population-based study including 555 asthmatics (Intermittent: 93, Mild: 240, Moderate: 158, and Severe: 64). The SNP was genotyped using allele-specific PCR. Obtained data were analyzed using the Generalized-Ordered Logit Estimates. Genotypes GA (OR: 0.53, CI: 0.32-0.90, P=0.019) and AA (OR: 0.22, CI: 0.06-0.76, P=0.017) were associated with decreased risk of Severe, Moderate, Mild vs. Intermittent stage; and Severe, Moderate, vs. Mild, Intermittent stages respectively. However, Genotype GA (OR: 1.90, CI: 1.05-3.44, P=0.033), dominant model GA+AA (OR: 2.04, CI: 1.17-3.57, P=0.012), and allele A (OR: 1.68, CI: 1.06-2.66, P=0.027) were associated with increased risk of Severe stage vs. Moderate, Mild, Intermittent stages. Also, male sex and higher age were associated with an increased odds ratio for severe asthma. Furthermore, significant associations with asthma stages were found for the interactions of the SNP and sex, smoking, and alcohol consumption. In conclusion, we revealed that the mutant allele of AhR-G1661A may interact with independent variables and act as a protective factor against lower stages of asthma but it may increase the risk of severe asthma.

وصف الملف: application/pdf

العلاقة: http://op.niscair.res.in/index.php/IJBB/article/view/59000Test

الإتاحة: http://op.niscair.res.in/index.php/IJBB/article/view/59000Test

المؤلفون: Per S. Daling, Trond Nordtug, Bjarne Kvæstad, Augustine Arukwe, Liv-Guri Faksness, Erika Oteri, Julia Farkas, Essa Ahsan Khan, Bjørn Henrik Hansen

المصدر: Aquatic Toxicology

مصطلحات موضوعية: Health, Toxicology and Mutagenesis, Developmental toxicity, 010501 environmental sciences, Aquatic Science, 01 natural sciences, 03 medical and health sciences, Diesel fuel, Gas oil, Cyp1a, Gadus, Animals, Marine ecosystem, Diesel, Ecosystem, 030304 developmental biology, 0105 earth and related environmental sciences, 0303 health sciences, biology, Chemistry, Heavy fuel oil, Fuel oil, biology.organism_classification, Aryl hydrocarbon receptor, Petroleum, Gadus morhua, Environmental chemistry, Toxicity, biology.protein, AhR gene battery, Embryotoxicity, Atlantic cod, Fuel Oils, Water Pollutants, Chemical

الوصف: Due to the heavy fuel oil (HFO) ban in Arctic maritime transport and new legislations restricting the sulphur content of fuel oils, new fuel oil types are continuously developed. However, the potential impacts of these new fuel oil types on marine ecosystems during accidental spills are largely unknown. In this study, we studied the toxicity of three marine fuel oils (two marine gas oils with low sulphur contents and a heavy fuel oil) in early life stages of cod (Gadus morhua). Embryos were exposed for 4 days to water-soluble fractions of fuel oils at concentrations ranging from 4.1 - 128.3 µg TPAH/L, followed by recovery in clean seawater until 17 days post fertilization. Exposure to all three fuel oils resulted in developmental toxicity, including severe morphological changes, deformations and cardiotoxicity. To assess underlying molecular mechanisms, we studied fuel oil-mediated activation of aryl hydrocarbon receptor (Ahr) gene battery and genes related to cardiovascular, angiogenesis and osteogenesis pathways. Overall, our results suggest comparable mechanisms of toxicity for the three fuel oils. All fuel oils caused concentration-dependant increases of cyp1a mRNA which paralleled ahrr, but not ahr1b transcript expression. On the angiogenesis and osteogenesis pathways, fuel oils produced concentration-specific transcriptional effects that were either increasing or decreasing, compared to control embryos. Based on the observed toxic responses, toxicity threshold values were estimated for individual endpoints to assess the most sensitive molecular and physiological effects, suggesting that unresolved petrogenic components may be significant contributors to the observed toxicity.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::311e885952dd383e7b3009ee1ab746dbTest
https://hdl.handle.net/11250/3025602Test