المؤلفون: Da-Wang Zhao, Bing Ren, Hong-Wei Wang, Xiao Zhang, Ming-Zhi Yu, Lei Cheng, Yuan-Hua Sang, Shuai-Shuai Cao, Florian M. Thieringer, Dong Zhang, Yi Wan, Chao Liu

المصدر: Bone & Joint Research, Vol 10, Iss 7, Pp 411-424 (2021)

مصطلحات موضوعية: interleukin 4, 3d-printed titanium implant, macrophage polarization, Diseases of the musculoskeletal system, RC925-935

الوصف: Aims: The use of 3D-printed titanium implant (DT) can effectively guide bone regeneration. DT triggers a continuous host immune reaction, including macrophage type 1 polarization, that resists osseointegration. Interleukin 4 (IL4) is a specific cytokine modulating osteogenic capability that switches macrophage polarization type 1 to type 2, and this switch favours bone regeneration. Methods: IL4 at concentrations of 0, 30, and 100 ng/ml was used at day 3 to create a biomimetic environment for bone marrow mesenchymal stromal cell (BMMSC) osteogenesis and macrophage polarization on the DT. The osteogenic and immune responses of BMMSCs and macrophages were evaluated respectively. Results: DT plus 30 ng/ml of IL4 (DT + 30 IL4) from day 3 to day 7 significantly (p < 0.01) enhanced macrophage type 2 polarization and BMMSC osteogenesis compared with the other groups. Local injection of IL4 enhanced new bone formation surrounding the DT. Conclusion: DT + 30 IL4 may switch macrophage polarization at the appropriate timepoints to promote bone regeneration.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2046-3758Test

الوصول الحر: https://doaj.org/article/4b24b836d9214c8d8a7f10add1218113Test

المؤلفون: Hongwei Wang, Lei Cheng, Shuaishuai Cao, Mingzhi Yu, Florian M. Thieringer, Dong Zhang, Yi Wan, Bing Ren, Da-Wang Zhao, Yuan-Hua Sang, Chao Liu, Xiao Zhang

المصدر: Bone & Joint Research
Bone & Joint Research, Vol 10, Iss 7, Pp 411-424 (2021)

مصطلحات موضوعية: 0303 health sciences, 3d printed, Titanium implant, Angiogenesis, Chemistry, macrophage polarization, Macrophage polarization, Diseases of the musculoskeletal system, 02 engineering and technology, 021001 nanoscience & nanotechnology, Interleukin 4, Cell biology, Biomaterials, 03 medical and health sciences, RC925-935, Macrophage, Orthopedics and Sports Medicine, Surgery, Immune reaction, 3D-printed titanium implant, 0210 nano-technology, Bone regeneration, 030304 developmental biology

الوصف: Aims The use of 3D-printed titanium implant (DT) can effectively guide bone regeneration. DT triggers a continuous host immune reaction, including macrophage type 1 polarization, that resists osseointegration. Interleukin 4 (IL4) is a specific cytokine modulating osteogenic capability that switches macrophage polarization type 1 to type 2, and this switch favours bone regeneration. Methods IL4 at concentrations of 0, 30, and 100 ng/ml was used at day 3 to create a biomimetic environment for bone marrow mesenchymal stromal cell (BMMSC) osteogenesis and macrophage polarization on the DT. The osteogenic and immune responses of BMMSCs and macrophages were evaluated respectively. Results DT plus 30 ng/ml of IL4 (DT + 30 IL4) from day 3 to day 7 significantly (p < 0.01) enhanced macrophage type 2 polarization and BMMSC osteogenesis compared with the other groups. Local injection of IL4 enhanced new bone formation surrounding the DT. Conclusion DT + 30 IL4 may switch macrophage polarization at the appropriate timepoints to promote bone regeneration. Cite this article: Bone Joint Res 2021;10(7):411–424.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::61318eef3203187f93d2b93b23e13346Test
https://doi.org/10.1302/2046-3758.107.bjr-2020-0334.r4Test