المؤلفون: Lalatović, Ninoslava, Ždralević, Maša, Antunović, Tanja, Pantović, Snežana

المصدر: Scientific Reports ; volume 13, issue 1 ; ISSN 2045-2322

مصطلحات موضوعية: Multidisciplinary

الوصف: Genetic factors are recognized as risk factors for statin-associated muscle symptoms (SAMS), which are the most common cause of statin intolerance. The aim of this study was to determine whether there is an association between polymorphisms 1236C > T, 2677G > T/A, and 3435C > T in the ABCB1 gene, encoding the efflux transporter of statins, and SAMS, as results on this topic are still controversial. A cross-sectional study was conducted on patients with or without SAMS using atorvastatin. The influence of non-genetic variables on SAMS was also evaluated. Our results show that patients with TT genotype in 1236C > T, 2677G > T/A, and 3435C > T polymorphisms had higher risk of developing SAMS, compared to wild type and heterozygous carriers together (OR 4.292 p = 0.0093, OR 5.897 p = 0.0023 and OR 3.547 p = 0.0122, respectively). Furthermore, TTT/TTT diplotype was also associated with a higher risk of SAMS, OR 9.234 ( p = 0.0028). Only family history of cardiovascular disease was found to be a risk factor for SAMS, in addition to the known non-genetic variables. We believe that ABCB1 genotyping has great potential to be incorporated into clinical practice to identify high-risk patients in a timely manner.

الإتاحة: https://doi.org/10.1038/s41598-023-44792-2Test
https://www.nature.com/articles/s41598-023-44792-2.pdfTest
https://www.nature.com/articles/s41598-023-44792-2Test

المؤلفون: Ždralević, Maša, Pouysségur, Jacques

المصدر: Frontiers in Oncology ; volume 13 ; ISSN 2234-943X

مصطلحات موضوعية: Cancer Research, Oncology

الإتاحة: https://doi.org/10.3389/fonc.2023.1226078Test

المؤلفون: Ždralević, Maša¹ (AUTHOR) masaz@ucg.ac.me, Musicco, Clara² (AUTHOR) c.musicco@ibiom.cnr.it, Giannattasio, Sergio² (AUTHOR) s.giannattasio@ibiom.cnr.it

المصدر: International Journal of Molecular Sciences. Jun2024, Vol. 25 Issue 12, p6344. 3p.

مصطلحات موضوعية: *MITOCHONDRIA, *BCL-2 proteins, *CYCLIC-AMP-dependent protein kinase, *MITOCHONDRIAL membranes, *HUMAN experimentation, *GENETIC translation

مستخلص: The International Journal of Molecular Sciences has published a special issue titled "Mitochondrial Research: Yeast and Human Cells as Models 2.0," which features six open-access papers that explore the intricate mechanisms of mitochondrial biology and dysfunction. The papers cover a range of topics, including the role of mitochondrial proteins in cellular processes, a new technique for measuring mitochondrial function in individual cells, the effects of viral infection on mitochondrial dynamics and respiratory chain function, the involvement of mitochondria in regulated cell death, the interplay between cell cycle regulation and mitochondrial metabolism, and the functions of long non-coding RNAs encoded by mitochondrial DNA. These findings contribute to our understanding of mitochondrial function and provide a foundation for the development of targeted therapeutic strategies. [Extracted from the article]

المؤلفون: Popovic, Natasa, Ždralević, Maša, Vujosevic, Stela, Radunović, Miroslav, Adžić Zečević, Antoaneta, Rovčanin Dragović, Isidora, Vukčević, Batrić, Popovic, Tomo, Radulović, Ljiljana, Vuković, Tijana, Eraković, Jevto, Lazović, Ranko, Radunović, Miodrag

المصدر: Biogerontology; Dec2023, Vol. 24 Issue 6, p971-985, 15p

مستخلص: Physiological changes associated with aging increase the risk for the development of age-related diseases. This increase is non-specific to the type of age-related disease, although each disease develops through a unique pathophysiologic mechanism. People who age at a faster rate develop age-related diseases earlier in their life. They have an older "biological age" compared to their "chronological age". Early detection of individuals with accelerated aging would allow timely intervention to postpone the onset of age-related diseases. This would increase their life expectancy and their length of good quality life. The goal of this study was to investigate whether retinal microvascular complexity could be used as a biomarker of biological age. Retinal images of 68 participants ages ranging from 19 to 82 years were collected in an observational cross-sectional study. Twenty of the old participants had age-related diseases such as hypertension, type 2 diabetes, and/or Alzheimer's dementia. The rest of the participants were healthy. Retinal images were captured by a hand-held, non-mydriatic fundus camera and quantification of the microvascular complexity was performed by using Sholl's, box-counting fractal, and lacunarity analysis. In the healthy subjects, increasing chronological age was associated with lower retinal microvascular complexity measured by Sholl's analysis. Decreased box-counting fractal dimension was present in old patients, and this decrease was 2.1 times faster in participants who had age-related diseases (p = 0.047). Retinal microvascular complexity could be a promising new biomarker of biological age. The data from this study is the first of this kind collected in Montenegro. It is freely available for use. [ABSTRACT FROM AUTHOR]

: Copyright of Biogerontology is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Schwab, Melissa, Thunborg, Katharina, Azimzadeh, Omid, von Toerne, Christine, Werner, Caroline, Shevtsov, Maxim, Di Genio, Tommaso, Zdralevic, Masa, Pouyssegur, Jacques, Renner, Kathrin, Kreutz, Marina, Multhoff, Gabriele

مصطلحات موضوعية: info:eu-repo/classification/ddc

وصف الملف: application/pdf

العلاقة: https://mediatum.ub.tum.de/1730543Test; https://mediatum.ub.tum.de/doc/1730543/document.pdfTest

الإتاحة: https://doi.org/10.3390/cancers13153762Test
https://mediatum.ub.tum.de/1730543Test
https://mediatum.ub.tum.de/doc/1730543/document.pdfTest

المؤلفون: Pouysségur, Jacques, Ždralević, Maša

المصدر: Journal of Biological Chemistry ; volume 294, issue 1, page 67 ; ISSN 0021-9258

مصطلحات موضوعية: Cell Biology, Molecular Biology, Biochemistry

الإتاحة: https://doi.org/10.1074/jbc.rl118.006868Test
https://api.elsevier.com/content/article/PII:S0021925820368770?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0021925820368770?httpAccept=text/plainTest

المؤلفون: Popovic, Natasa, Vujosevic, Stela, Radunovic, Miroslav, Adžić Zečević, Antoaneta, Ždralević, Maša, Rovčanin Dragović, Isidora, Vukčević, Bartić, Popovic, Tomo, Radulović, Ljiljana, Vuković, Tijana, Eraković, Jevto, Lazović, Ranko, Radunović, Miodrag

الوصف: Introduction Topological characterization of the Retinal microvascular nEtwork visualized by portable fuNDus camera (TREND 2) is a database of digital color eye fundus images created as an addition to TREND database (https://zenodo.org/badge/DOI/10.5281/zenodo.4521044.svgTest). TREND 2 databse was created by medical professionals of the Faculty of Medicine of the University of Montenegro in 2023. Purpose 1) to provide a standard that defines normal and abnormal retinal anatomy and microvascular geometry as it appears when visualized by the portable fundus camera 2) to help the development of new methods for stratification of the risk for the development of various eye diseases, as well as systemic diseases that affect microvasculature 3) to aid the development of biomarkers of accelerated aging 4) to provide a standard that can be used to develop software for segmentation of retinal microvasculature, grading the quality of retinal digital images, and computer-aided diagnosis of systemic and chronic diseases. All color digital images were acquired with a hand-held portable, non-mydriatic MiiS HORUS Scope DEC 200 with 45º FOV and 2560 X 1920 pixel resolution. Data The TREND public database contains 28 color fundus images of old subjects (20 images from subjects with one or more chronic diseases such as type 2 diabetes mellitus, hypertension or Alzheimer's dementia- O_CD group, and 8 images from subjects with no chronic diseases- O_NCD group). Each image is associated with a corresponding binarized image of the manually segmented microvascular network. Inclusion and Exclusion Criteria O_NCD group O_CD group Inclusion Criteria Inclusion Criteria - at least 56 years old - at least 56 years old - no current acute disease - no history of alcohol, or drug abuse, or psychiatric disease - no current acute disease - no history of alcohol, or drug abuse, or psychiatric disease - no history of alcohol, or drug abuse, or psychiatric disease - no history of alcohol, or drug abuse, or psychiatric disease - negative history of any ...

العلاقة: https://zenodo.org/record/7678656Test; https://doi.org/10.5281/zenodo.7678656Test; oai:zenodo.org:7678656

الإتاحة: https://doi.org/10.5281/zenodo.7678656Test
https://doi.org/10.5281/zenodo.7678655Test
https://zenodo.org/record/7678656Test

المؤلفون: Ždralević, Maša, Brand, Almut, Di Ianni, Lorenza, Dettmer, Katja, Reinders, Jörg, Singer, Katrin, Peter, Katrin, Schnell, Annette, Bruss, Christina, Decking, Sonja-Maria, Koehl, Gudrun, Felipe-Abrio, Blanca, Durivault, Jérôme, Bayer, Pascale, Evangelista, Marie, O'Brien, Thomas, Oefner, Peter J., Renner, Kathrin, Pouysségur, Jacques, Kreutz, Marina

المساهمون: Fondation ARC pour la Recherche sur le Cancer

المصدر: Journal of Biological Chemistry ; volume 293, issue 41, page 15947-15961 ; ISSN 0021-9258

الإتاحة: https://doi.org/10.1074/jbc.ra118.004180Test
https://api.elsevier.com/content/article/PII:S0021925820351358?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0021925820351358?httpAccept=text/plainTest

المؤلفون: Ždralević, Maša, Vučetić, Milica, Daher, Boutaina, Marchiq, Ibtissam, Parks, Scott K., Pouysségur, Jacques

المساهمون: Fondation ARC on Cancer Research, SERVIER-CNRS, LNCC, Centre Scientifique de Monaco, GEMLUC, Université Côte d Azur, IRCAN, Centre A. Lacassagne

المصدر: Advances in Biological Regulation ; volume 68, page 55-63 ; ISSN 2212-4926

مصطلحات موضوعية: Cancer Research, Genetics, Molecular Biology, Molecular Medicine

الإتاحة: https://doi.org/10.1016/j.jbior.2017.12.002Test
https://api.elsevier.com/content/article/PII:S2212492617301896?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2212492617301896?httpAccept=text/plainTest

المؤلفون: Guaragnella, Nicoletta, Ždralević, Maša, Palková, Zdena, Giannattasio, Sergio

المساهمون: Guaragnella N, Ždralević M, Palková Z, Giannattasio S., Guaragnella, Nicoletta, Ždralević, Maša, Palková, Zdena, Giannattasio, Sergio

مصطلحات موضوعية: ACO1, CIT2, IDH1, Mitochondrial retrograde pathway, RTG gene, Yeast

الوصف: Mitochondrial retrograde signaling is a mitochondria-to-nucleus communication pathway, conserved from yeast to humans, by which dysfunctional mitochondria relay signals that lead to cell stress adaptation in physiopathological conditions via changes in nuclear gene expression. The most comprehensive picture of components and regulation of retrograde signaling has been obtained in Saccharomyces cerevisiae, where retrograde-target gene expression is regulated by RTG genes. In this chapter, we describe methods to measure mitochondrial retrograde pathway activation at the level of mRNA and protein products in yeast model systems, including cell suspensions and microcolonies. In particular, we will focus on three major procedures: mRNA levels of RTG-target genes, such as those encoding for peroxisomal citrate synthase (CIT2), aconitase, and NAD+-specific isocitrate dehydrogenase subunit 1 by real-time PCR; expression analysis of CIT2-gene protein product (Cit2p-GFP) by Western blot and fluorescence microscopy; the phosphorylation status of transcriptional factor Rtg1/3p which controls RTG-target gene transcription.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/34060034; ispartofbook:Mitochondrial Medicine; volume:2276; firstpage:87; lastpage:102; numberofpages:16; https://hdl.handle.net/11586/415694Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85107197435

الإتاحة: https://doi.org/10.1007/978-1-0716-1266-8_6Test
https://hdl.handle.net/11586/415694Test