التفاصيل البيبلوغرافية
| العنوان: |
Early mechanisms of neutrophil activation and transmigration in acute lung injury. |
| المؤلفون: |
Cagle, L. A., Linderholm, A. L., Franzi, L. M., Last, J. A., Simon, S. I., Kenyon, N. J., Harper, R. W. |
| المصدر: |
Frontiers in Physiology; 12/21/2022, Vol. 13, p01-09, 9p |
| مصطلحات موضوعية: |
LUNG injuries, NEUTROPHILS, POSITIVE end-expiratory pressure, SALMONELLA enterica |
| مصطلحات جغرافية: |
NEW York (N.Y.), MONTREAL (Quebec) |
| الشركة/الكيان: |
UNIVERSITY of California, Davis |
| مستخلص: |
Introduction: Neutrophil transmigration is multifactorial and primarily driven by selectins and β2-integrins (CD11b/CD18), whose expression are dependent on the underlying stimulus. Ventilator-induced lung injury (VILI) results in a predominantly CD18-independent mechanism of neutrophil recruitment, while direct endotoxin-induced lung injury results from a CD18-dependent mechanism. We previously observed that lack of NADPH oxidases DUOX1 and DUOX2 resulted in reduced neutrophil influx in a VILI model of lung injury but had no influence on neutrophil influx after LPS exposure. Based on these observations, we hypothesized that DUOX1/DUOX2 are an important component of CD18-independent mechanisms of neutrophil recruitment in the lung. Methods: We exposed Duoxa-/- (KO) mice and Duoxa+/+ (WT) mice to either an intratracheal exposure of lipopolysaccharide (LPS/endotoxin)-or high tidal volume ventilation and compared expression of neutrophil markers between groups. WT mice (129S6/SvEvTac) were obtained from Taconic Biosciences (One Discovery Drive Suite 304; Rensselaer, NY 1244) and were allowed to acclimatize for one week prior to study enrollment. KO mice were generated as previously described [Grasberger 2012] and bred in-house on a 129S6 background. We provided positive-pressure ventilation at a tidal volume of 10 ml/kg with 2 cmH20 positive end-expiratory pressure (PEEP). Mice were assigned to groups consisting of KO (n = 5) and WT (n = 5) in each group and divided into non-ventilated, positive-pressure ventilation, or LPS IT exposure groups. Positive-pressure ventilation was instituted for 4-h using a FlexiVent (Flexiware 8.1, Scireq, Montreal, QC, Canada). Lipopolysaccharide (Salmonella enterica serotype tryphimurium L6143, Millipore Sigma) was administered via an intratracheal (IT) route at a dose of 0.1 mg/kg. Mice were humanely euthanized at 4-h post-injection consistent with the UC Davis IAUCAC-approved protocol. Results:As previously observed, neutrophilic influx into the airways was significantly impaired in the Duoxa-/- (KO) mice after VILI, but not after LPS exposure. LPS-induced lung injury resulted in upregulation of CD11b+ neutrophils and shedding of CD62L and CD162 regardless of DUOX expression, whereas VILI resulted in upregulation of CD49+ neutrophils in the Duoxa+/+ (WT) mice but not the Duoxa-/- (KO) mice. Conclusion: Our data suggest DUOX is required for CD18-independent mechanisms of neutrophil recruitment in the lung induced by acute lung injury, but not for canonical CD18-depedent mechanisms after LPS exposure. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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