رسالة جامعية
Development of a successful method for quantifying viable oral anaerobic spirochetes from pure culture and periodontal pockets
| العنوان: | Development of a successful method for quantifying viable oral anaerobic spirochetes from pure culture and periodontal pockets |
|---|---|
| المؤلفون: | Qiu, Yu-Shan |
| مرشدي الرسالة: | Chan, Z. C. S. (advisor) |
| بيانات النشر: | McGill University, 1993. |
| سنة النشر: | 1993 |
| المجموعة: | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| مصطلحات موضوعية: | Biology, Microbiology., Health Sciences, Dentistry. |
| جغرافية الموضوع: | Master of Science (Department of Microbiology and Immunology.) |
| الوصف: | Spirochetes are markedly prevalent in periodontal disease but are not included as predominant cultivable organisms because of the inability to quantify them by viable count. A successful method was developed for enumerating viable oral spirochetes in pure culture as colony-forming units (CFU) in new oral spirochete (NOS) medium with 0.7% agarose and using small tissue-culture flasks. Three species of oral spirochetes in log-phase growth in NOS broth were used for evaluation of the method. Reliable, consistent and reproducible viable counts of pure spirochete cultures were obtained. This method was extended to enumerate viable oral spirochetes from periodontal pockets. The antibiotic rifampin (20 $ mu$g/ml) was found to be an excellent selective agent for such a count when added to NOS-agarose medium. Counts of cultivable oral spirochetes from 10 subgingival plaque samples ranged from 12.5% to 28.2% of the total cultivable anaerobic bacteria. In addition, by the use of this method thirteen new oral spirochetes were isolated. The viable count technique was modified and employed to study the locomotion of spirochetes. Migration of oral spirochetes out of NOS-Bacto agar medium into NOS-agarose medium was observed and two locomotory phenotypes of oral spirochetes were detected. |
| Original Identifier: | oai:collectionscanada.gc.ca:QMM.68246 |
| نوع الوثيقة: | Electronic Thesis or Dissertation |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | alephsysno: 001397531; proquestno: AAIMM94504; Theses scanned by UMI/ProQuest. |
| الإتاحة: | http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=68246Test |
| حقوق: | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| رقم الانضمام: | edsndl.LACETR.oai.collectionscanada.gc.ca.QMM.68246 |
| قاعدة البيانات: | Networked Digital Library of Theses & Dissertations |
| الوصف غير متاح. |