التفاصيل البيبلوغرافية
| العنوان: |
A polyphasic characterisation of Aspergillus section Flavi isolated from Malaysian and Nigerian food grains and poultry feeds by phenotypic, chemotypic, and molecular methods. |
| المؤلفون: |
Salisu, Baha'uddeen, Anua, Siti Marwanis, Wan Rosli, Wan Ishak, Mazlan, Nurzafirah |
| المصدر: |
Biocatalysis & Agricultural Biotechnology; Jun2024, Vol. 58, pN.PAG-N.PAG, 1p |
| مصطلحات موضوعية: |
POULTRY as food, PHENOTYPES, HIGH performance liquid chromatography, ASPERGILLUS, POLYMERASE chain reaction |
| مصطلحات جغرافية: |
KELANTAN, KATSINA (Nigeria) |
| مستخلص: |
The taxonomy and toxigenicity of Aspergillus section Flavi (ASF) in various regions are still under-reported, as many potentially toxic species are continuously discovered. This study aims to determine the aflatoxigenicity and identify 74 isolates of ASF from Malaysian and Nigerian food grains and feeds using phenotypic, chemotypic, and molecular methods. Isolates were screened for sclerotium morphotype and aspergillic acid production phenotypically, while an ultra-fast reverse-phase high-performance liquid chromatography (HPLC) was optimised to quantify the cyclopiazonic acid and aflatoxins produced by the isolated ASF, based on which, they were characterised into phenotypes L-strains, S-strains, and NS-strains, and chemotypes I to VI. Chemotypic data and nucleotide sequences of β-tubulin and internal transcribed spacer (ITS) genes were utilised to identify the isolates' species and determine their phylogenetic relationships. Aflatoxigenesis on solid media and multiplex polymerase chain reactions of four essential genes (aflR, aflM, aflD, and aflP) of aflatoxin biosynthesis were used to confirm the aflatoxigenicity of each isolate. All the six chemotypes of ASF were present among the isolated strains, with the aflatoxigenic chemotypes (55.41%) and CPA-positive isolates (68.92%) being significantly higher (p < 0.05) than the non-aflatoxigenic and CPA negative chemotypes. Aflatoxins and CPA were produced in the 0.25–19.58 ng/g range and 29.89–46.55 ng/g, respectively. Chemotypic data accurately differentiate toxigenic from atoxigenic species but only identify 90% of species names. Molecular data accurately resolved species but not toxigenicity. The results highlight the need for robust regulatory measures in the concerned regions to mitigate the risk of aflatoxicosis. • Major exometabolites (Aflatoxin B1, B2, G1, G2, Cyclopiazonic acid and Aspergillic acid) from Aspergillus section Flavi (ASF) isolated in poultry feeds, food grains, and peanuts consumed in Kelantan state (Malaysia) and Katsina state (Nigeria) were determined using an optimised ultra-fast reverse-phase high-performance liquid chromatography with photodiode array detector (UF-RP-HPLC-DAD). • Species names and phylogenetic relationships of the isolated ASF were determined through PCR amplification and nucleotide sequencing analysis of their ITS and β-tubulin genes. • Chemotyping and aflatoxigenicity of the isolated ASF were carried out using extrolites data and multiplex PCR screening of the isolates for essential aflatoxin biosynthesis genes (ABGs), AflD, AflM, AflP and AflR genes. • Being the first study of this nature from Katsina state and Kelantan state, the polyphase data generated (extrolites data, ITS and β-tubulin genes' nucleotide sequences, Multiplex PCR of the ABGs) will serve as reference data for the incidence/prevalence of aflatoxigenic and atoxigenic ASF in poultry feeds, cereals and peanuts. and will help in evaluating the safety of foods and feeds, as well as generating and predicting the degree of fungal and aflatoxin contamination of those products in Katsina and Kelantan for effective aflatoxin control. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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