المؤلفون: Sarshari, Behrang, Mohebbi, Seyed Reza, Ravanshad, Mehrdad, Shahrokh, Shabnam, Aghdaei, Hamid Asadzadeh

المصدر: Gastroenterology & Hepatology from Bed to Bench; Summer2022, Vol. 15 Issue 3, p225-231, 7p

مصطلحات موضوعية: STOMACH tumors, SEQUENCE analysis, BIOPSY, DNA, PAPER chromatography, MICROBIAL genetics, GASTRITIS, GENETIC variation, EPSTEIN-Barr virus, DESCRIPTIVE statistics, POLYMERASE chain reaction

مصطلحات جغرافية: IRAN

مستخلص: Aim: The current study aimed to investigate sequence variations in the C-terminus of latent membrane protein 1 (LMP1) in Epstein- Barr virus (EBV) isolates from Iranian patients with chronic gastritis or gastric cancer (GC). Background: LMP1, an essential viral oncoprotein, is the critical element in the immortalization of B cells. It contains a small twenty-four amino acid cytoplasmic N-terminal region, six transmembrane segments, and a two hundred amino acid cytoplasmic Cterminal domain. Most LMP1-mediated signal transduction events are moderated by some functional parts of the cytoplasmic Cterminal domain. Methods: Thirty-two EBV-positive biopsy tissues were obtained from patients with gastric cancer and patients with chronic gastritis. The C-terminal nucleotide sequences of LMP1 were amplified using nested-PCR and analyzed by DNA sequencing. Results Four to eight copies of the 11 repeat elements (codon 254--302) were observed in the carboxyl-terminal site of patients, but no relationship was found between the number of repeat sequences and disease status. The 30-bp deletion corresponding to codon 345--354 of the B95-8 strain was observed in 34% of isolates, and the remaining samples were non-deleted. In the gastric cancer group, a higher number of 33-bp repeats (≥5 repeats) was observed in 30-bp-deletion (100%) than in non-deleted (42%) isolates, and the difference was statistically significant. Analysis revealed that a gastritis isolate may be the result of recombination between Alaskan and China1 strains. Conclusion: Overall, the current results showed no association between C-terminal sequence variations of LMP1 and malignant or non-malignant isolate origin. [ABSTRACT FROM AUTHOR]

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المؤلفون: Faryabi, E., Noori, M., Mousavi, A., Jafari, A.

المصدر: Journal of Applied Horticulture; Jan-Apr2022, Vol. 24 Issue 1, p22-26, 5p

مصطلحات موضوعية: ESSENTIAL oils, FLAVONOIDS, THYMUS, STACHYS, PAPER chromatography, FLAVONOLS, PINENE

مصطلحات جغرافية: IRAN, ZAGROS Mountains (Iran & Iraq)

مستخلص: Iran has different environmental conditions due to its specific geographical location. Therefore, exploring essential oil and flavonoid components among medicinal plants in different areas may result in discoveries about valuable bioactive compounds. The present investigation aimed to study flavonoids profile and essential oils composition of four Lamiaceae species i.e., Stachys lavandulifolia, Teucrium polium, Thymus daenensis and Ziziphora clinopodioides from Oshtorankoh located on Zagros Mountains, Iran. A chemical study using two dimentional paper chromatography (2-DPC) and thin-layer chromatography (TLC) showed that the four studied species contained flavone C and C-/O glucosides and flavonoid sulphates. The present study revealed that T. polium had high levels of many flavonoids such as apigenin, genistein, isorhamnetin, kaempferol, quercetin, rutin, and vitexin compared to the other studied plants. The results of GC/MS analysis showed that S. lavandulifolia was rich in a-pinene (21.16 %) and 1,8-cineol (16.95 %), T. polium in a-pinene (12.21 %) and sabinene (9.55 %), T. daenensis in α-Terpinene (36.98 %) and p-Cymene (26.87 %), and Z. clinopodioides in piperitone (16.84 %) and 1,8-cineol (16.11 %) components. [ABSTRACT FROM AUTHOR]

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العنوان البديل: Detection and Relation of Polyketide Synthase (PKSs) Genes Responsible for Antimicrobial Activity in Terrestrial Cyanobacteria of Lavasan. (English)

المؤلفون: رقیهسادات حسینی, عباس اخوانسپهی, بهاره نوروزی

المصدر: Iranian Journal of Medical Microbiology; Jan/Feb2019, Vol. 12 Issue 6, p419-431, 13p

مصطلحات موضوعية: ANTI-infective agents, BACTERIA, BIOLOGICAL assay, GENES, GENETIC techniques, ORGANIC compounds, PAPER chromatography, PEPTIDES, PHYLOGENY, SEQUENCE analysis

مصطلحات جغرافية: IRAN

مستخلص: Background and Aims: Cyanobacteria are considered as favorable source for new pharmaceutical compounds. To date, the majority of bioactive metabolites isolated from cyanobacteria are either polyketides (PKSs) or non-ribosomal peptides. Despite of several worldwide studies on prevalence of PKSs, none of them included the terrestrial cyanobacteria of the Lavasan. Therefore, this study aimed to detectthe PKS genes and correlation of the presence of these genes with antimicrobial compounds synthesis. Materials and Methods: Morphological and molecular identification of the terrestrial strains was performed after culture and purification. Amplification of polyketide synthase and phylogenetic trees were used for phylogenetic analysis. Nucleotide and protein sequences were deposited in GenBank. Lastly, to show the correlation of this gene with antimicrobial compounds synthesis, antibiogram bioassay was used. Results: Phylogentic analysis revealed that most of the identified 16SrRNA genes and PKS domains had more than 90% similarity to their closest matches in the Gen-Bank. In addition, antibiogram assessment showedthe different pattern of inhibition, indicating the involvement of variety antimicrobial substances. Conclusions: According to the results of this sudy, it seems the antibiogram bioassay and molecular detection of polyketide synthase genes are useful techniques for the assessment of productive species of natural products and the possible role of polyketide synthase enzyme complexes in the biosynthesis of biologically active compounds. [ABSTRACT FROM AUTHOR]

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المؤلفون: Shirmardi, Seyed Pezhman, Gandomkar, Mostafa, Shamsaei, Mojtaba, Mirakabadi, Abbas Zare, Maragheh, Mohammad Ghannadi, Shafiei, Mohammad, Vahidfar, Nasim

المصدر: Iranian Journal of Nuclear Medicine; 2010, Vol. 18 Issue 1, p37-44, 8p

مصطلحات موضوعية: MESOBUTHUS eupeus, SCORPION venom, LABORATORY mice, PAPER chromatography, RADIOLABELING

مصطلحات جغرافية: IRAN

مستخلص: Introduction: Iranian scorpion species are classified in Buthidae and Scorpionidae with 16 genera and 25 species. In Iran, similar to other parts of the world, there are a few known species of scorpions responsible for severe envenoming. Mesobuthus eupeus is the most common species in Iran. Its venom contains several toxin fractions which can affect the ion channel. In this study purification, labeling and biological evaluation of Mesobuthus eupeus scorpion venom are described. Methods: To separate different venom fractions, soluble venom was loaded on a chromatography column packed with sephadex G50 gel then the fractions were collected according to UV absorption at 280 nm wavelength. Toxic fraction (F3) was loaded on anionic ion exchanger resin (DEAE) and then on a cationic resins (CM). Finally toxic fraction F319 was labeled with ^99mTc and radiochemical analysis was determined by paper chromatography. The biodistribution was studied after injection into normal mice. Results: Toxic fraction of venom was successfully obtained in purified form. Radiolabeling of venom was performed at high specific activity with radiochemical purity more than 95% which was stable for more than 4 h. Biodistribution studies in normal mice showed rapid clearance of compound from blood (2.64% ID at 4 h) and tissues except the kidneys (27% ID at 4 h). Conclusion: As tissue distribution studies are very important for clinical use, results of this study suggest that ^99mTc labeling of venom can be a useful tool for in vivo studies and is an excellent approach to follow the process of biodistribution and kinetics of toxins. [ABSTRACT FROM AUTHOR]

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المؤلفون: Noori, M., Malayeri, B. E., Jafari, M.

المصدر: Toxicological & Environmental Chemistry; Apr2009, Vol. 91 Issue 3, p409-418, 10p, 1 Chart, 2 Graphs

مصطلحات موضوعية: FLAVONOIDS, LEGUMES, FLUORIDES, CHROMATOGRAPHIC analysis, THIN layer chromatography, PAPER chromatography

مصطلحات جغرافية: IRAN

مستخلص: The effects of fluoride (Fl) emission, from an aluminum (Al) reduction plant in Iran, on the concentration of Fl in six legume species were studied. In addition, the influence of Fl on leaf flavonoids was investigated. Determination of Fl in collected plants (Alhagi camelorum Fisch., Cercis siliquastrum L., Glycyrrhiza glabra L. Medicago sativa L., Robinia peseudoacacia L., and Sophora alopecuroides L.) from the factory area was performed using a Fl ion-selective electrode. Identification of flavonoids in the samples was obtained by two-dimensional paper chromatography (2-D PC) and thin layer chromatography (TLC). Controls were obtained from plants located 10 km distance from the factory. Results showed high Fl concentration in all polluted samples. Phytochemical changes in polluted leaves were observed with appearance or disappearance of some flavonoids. [ABSTRACT FROM AUTHOR]

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المؤلفون: Nickavar, Bahman, Amin, Gholamreza

المصدر: Pharmaceutical Biology; Jun2004, Vol. 42 Issue 4/5, p289-291, 3p

مصطلحات موضوعية: ANTHOCYANINS, CAUCASIAN whortleberry, ERICACEAE, PAPER chromatography, CHROMATOGRAPHIC analysis

مصطلحات جغرافية: IRAN

مستخلص: The systematic investigation of the berries of Vaccinium arctostaphylos L. (Ericaceae), a native medicinal plant growing in Iran, permitted the identification of three major anthocyanins. The compounds were isolated from the acidified extract of the berries using the repeated paper chromatography with different mobile phases and were characterized using chromatographic, chemical, and spectroscopic methods as delphinidin 3- O -β-glucoside, petunidin 3- O -β-glucoside, and malvidin 3- O -β-glucoside. [ABSTRACT FROM AUTHOR]

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