التفاصيل البيبلوغرافية
| العنوان: |
Effect of Doxycycline-Release Anastomotic Augmentation Ring on Porcine Colorectal Anastomosis. |
| المؤلفون: |
Luchtefeld, Martin1 (AUTHOR) Martin.Luchtefeld@spectrumhealth.org, Jrebi, Nezar1,2 (AUTHOR), Hostetter, Galen3 (AUTHOR), Osterholzer, Kathy4 (AUTHOR), Dykema, Karl1 (AUTHOR), Khoo, Sok Kean1,5 (AUTHOR) khoos@gvsu.edu |
| المصدر: |
Journal of Surgical Research. Nov2022, Vol. 279, p464-473. 10p. |
| مصطلحات موضوعية: |
*MATRIX metalloproteinase inhibitors, *STAPLERS (Surgery), *GENE expression |
| مصطلحات جغرافية: |
HAMPSHIRE (England) |
| مستخلص: |
Collagen degradation can lead to early postoperative weakness in colorectal anastomosis. Matrix metalloproteinase inhibitors (MMPIs) are shown to decrease collagen breakdown and enhance healing in anastomosis in animal models. Here, we evaluated the effectiveness of a novel anastomotic augmentation ring (AAR) that releases doxycycline, an MMPI, from a poly(lactic-co-glycolic) acid ring in porcine anastomoses. Two end-to-end stapled colorectal anastomoses were performed in 20 Yorkshire-Hampshire pigs. AAR was randomly incorporated into either the proximal or distal anastomosis as treatment, while nonaugmented anastomosis served as a control. Animals were then euthanized on days 3, 4, and 5 before anastomosis explantation and burst pressure measurement. Each anastomosis site was also collected for histology, hydroxyproline content, and gene expression microarray analyses. No abscess or anastomotic leak was detected. Average burst pressures were not significantly different at any time point. There is no statistical difference in collagen content between the treatment group and controls. Gene expression analysis revealed no statistically significant in differentially expressed genes. However, genes related to inflammation, such as C-C motif chemokine ligand 11 (CCL11), CD70, and C-X-C motif chemokine ligand 10 (CXCL10), were upregulated (not statistically significant) in AAR compared to non-AAR anastomosis sites on days 3 and 4. This pilot study shows that doxycycline-release AAR is feasible and safe. While burst pressure and collagen content did not change significantly with doxycycline treatment, upregulating genes related to the inflammatory process for pathogen and debris clearance in AAR may improve the early stage of colorectal anastomotic healing. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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