دورية أكاديمية
Oxidized alginate hydrogels with the GHK peptide enhance cord blood mesenchymal stem cell osteogenesis: A paradigm for metabolomics-based evaluation of biomaterial design
العنوان: | Oxidized alginate hydrogels with the GHK peptide enhance cord blood mesenchymal stem cell osteogenesis: A paradigm for metabolomics-based evaluation of biomaterial design |
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المؤلفون: | Klontzas, ME, Reakasame, S, Silva, R, Morais, JCF, Vernardis, S, MacFarlane, RJ, Heliotis, M, Tsiridis, E, Panoskaltsis, N, Boccaccini, AR, Mantalaris, A |
المساهمون: | Commission of the European Communities |
المصدر: | 240 ; 224 |
بيانات النشر: | Elsevier |
سنة النشر: | 2019 |
المجموعة: | Imperial College London: Spiral |
مصطلحات موضوعية: | Science & Technology, Technology, Engineering, Biomedical, Materials Science, Biomaterials, Metabolomics, Metabolism, Hydrogels, CHK, Mesenchymal stem cells, Osteogenic differentiation, INTEGRIN-LINKED KINASE, IN-VITRO, EXTRACELLULAR-MATRIX, STROMAL CELLS, BONE-MARROW, BIOACTIVE GLASS, DIFFERENTIATION, GELATIN, EXPRESSION, GHK, Biomedical Engineering |
جغرافية الموضوع: | England |
الوصف: | Oxidized alginate hydrogels are appealing alternatives to natural alginate due to their favourable biodegradability profiles and capacity to self-crosslink with amine containing molecules facilitating functionalization with extracellular matrix cues, which enable modulation of stem cell fate, achieve highly viable 3-D cultures, and promote cell growth. Stem cell metabolism is at the core of cellular fate (proliferation, differentiation, death) and metabolomics provides global metabolic signatures representative of cellular status, being able to accurately identify the quality of stem cell differentiation. Herein, umbilical cord blood mesenchymal stem cells (UCB MSCs) were encapsulated in novel oxidized alginate hydrogels functionalized with the glycine-histidine-lysine (GHK) peptide and differentiated towards the osteoblastic lineage. The ADA-GHK hydrogels significantly improved osteogenic differentiation compared to gelatin-containing control hydrogels, as demonstrated by gene expression, alkaline phosphatase activity and bone extracellular matrix deposition. Metabolomics revealed the high degree of metabolic heterogeneity in the gelatin-containing control hydrogels, captured the enhanced osteogenic differentiation in the ADA-GHK hydrogels, confirmed the similar metabolism between differentiated cells and primary osteoblasts, and elucidated the metabolic mechanism responsible for the function of GHK. Our results suggest a novel paradigm for metabolomics-guided biomaterial design and robust stem cell bioprocessing. STATEMENT OF SIGNIFICANCE: Producing high quality engineered bone grafts is important for the treatment of critical sized bone defects. Robust and sensitive techniques are required for quality assessment of tissue-engineered constructs, which result to the selection of optimal biomaterials for bone graft development. Herein, we present a new use of metabolomics signatures in guiding the development of novel oxidised alginate-based hydrogels with umbilical cord blood mesenchymal stem cells and the ... |
نوع الوثيقة: | article in journal/newspaper |
اللغة: | English |
تدمد: | 1742-7061 |
العلاقة: | Acta Biomaterialia; http://hdl.handle.net/10044/1/67770Test; https://doi.org/10.1016/j.actbio.2019.02.017Test; 675585; 340719 |
DOI: | 10.1016/j.actbio.2019.02.017 |
الإتاحة: | https://doi.org/10.1016/j.actbio.2019.02.017Test http://hdl.handle.net/10044/1/67770Test |
حقوق: | © 2019 Acta Materialia Inc. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0Test/). |
رقم الانضمام: | edsbas.41090B2D |
قاعدة البيانات: | BASE |
تدمد: | 17427061 |
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DOI: | 10.1016/j.actbio.2019.02.017 |