دورية أكاديمية
Improved Method for Efficient Generation of Functional Neurons from Murine Neural Progenitor Cells.
| العنوان: | Improved Method for Efficient Generation of Functional Neurons from Murine Neural Progenitor Cells. |
|---|---|
| المؤلفون: | Soni, Abhinav, Klütsch, Diana, Hu, Xin, Houtman, Judith, Rund, Nicole, McCloskey, Asako, Mertens, Jerome, Schafer, Simon T, Amin, Hayder, Toda, Tomohisa |
| المصدر: | Cells 10(8), 1894 - (2021). doi:10.3390/cells10081894 |
| بيانات النشر: | MDPI |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | info:eu-repo/classification/ddc/570, Animals, Cell Culture Techniques, Cell Line, Electrical Synapses: physiology, Evoked Potentials, Gene Expression Regulation, Developmental, Mice, Inbred C57BL, Nerve Net: physiology, Neural Stem Cells: physiology, Neurogenesis: genetics, Phenotype, Synaptic Transmission, induced neurons, neural stem cells, neuronal culture, neuronal network |
| جغرافية الموضوع: | DE |
| الوصف: | Neuronal culture was used to investigate neuronal function in physiological and pathological conditions. Despite its inevitability, primary neuronal culture remained a gold standard method that requires laborious preparation, intensive training, and animal resources. To circumvent the shortfalls of primary neuronal preparations and efficiently give rise to functional neurons, we combine a neural stem cell culture method with a direct cell type-conversion approach. The lucidity of this method enables the efficient preparation of functional neurons from mouse neural progenitor cells on demand. We demonstrate that induced neurons (NPC-iNs) by this method make synaptic connections, elicit neuronal activity-dependent cellular responses, and develop functional neuronal networks. This method will provide a concise platform for functional neuronal assessments. This indeed offers a perspective for using these characterized neuronal networks for investigating plasticity mechanisms, drug screening assays, and probing the molecular and biophysical basis of neurodevelopmental and neurodegenerative diseases. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/pmid:34440662; info:eu-repo/semantics/altIdentifier/issn/2073-4409; https://pub.dzne.de/record/155862Test; https://pub.dzne.de/search?p=id:%22DZNE-2021-01022%22Test |
| الإتاحة: | https://doi.org/10.3390/cells10081894Test https://pub.dzne.de/record/155862Test https://pub.dzne.de/search?p=id:%22DZNE-2021-01022%22Test |
| حقوق: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.43B9E122 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |