التفاصيل البيبلوغرافية
| العنوان: |
Comparative evaluation of two immunoassays for serological diagnosis of hepatitis E. |
| المؤلفون: |
Sampedro, Antonio, Casanovas, Isabel, Ceballos, Julian, Rodriguez‐Granger, Javier, Cobo, Fernando, Navarro, Jose Maria |
| المصدر: |
Journal of Medical Virology; Feb2020, Vol. 92 Issue 2, p260-262, 3p |
| مصطلحات موضوعية: |
HEPATITIS E, IMMUNOASSAY, ENZYME-linked immunosorbent assay, HEPATITIS E virus, IMMUNOGLOBULIN M, HEPATITIS B |
| مصطلحات جغرافية: |
BEIJING (China), GRANADA (Spain) |
| مستخلص: |
Serological testing for immunoglobulin M (IgM) antibodies to Hepatitis E virus (HEV) is useful for diagnosing acute hepatitis E. Our aim was to evaluate the performance of chemiluminescence immunoassay (HEV IgM; Vircell, Granada, Spain) in comparison with a manual enzyme immunoassay (Anti‐HEV virus; Wantai, Beijing, China) for anti‐HEV IgM detection. Seventeen HEV‐RNA positive and 69 HEV‐RNA negative sera were tested by both assays. Virclia sensitivity was 94.1%, whereas that of the enzyme immunosorbent assay (EIA) was 76.5%; specificity was 98.5% and 97.1% for Virclia and EIA, respectively. Differences between both assays were not statistically significant. Virclia HEV IgM showed excellent performance and can be a good alternative to conventional enzyme immunoassays for the detection of IgM against HEV. Highlight: We have compared the diagnostic performance of a chemiluminiscence immunoassay (HEV IgM; Vircell, Granada, Spain) in comparison with a manual enzyme immunoassay (Wantai® Anti‐HEV Virus; Beijing, China) for serological diagnosis of hepatitis E. Both kits showed good sensitivity and specificity values and no significant differences were observed. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
