التفاصيل البيبلوغرافية
| العنوان: |
Vitamin B6 Deficiency Promotes Loss of Heterozygosity (LOH) at the Drosophilawarts (wts) Locus |
| المؤلفون: |
Eleonora Gnocchini, Eleonora Pilesi, Ludovica Schiano, Fiammetta Vernì |
| المصدر: |
International Journal of Molecular Sciences; Volume 23; Issue 11; Pages: 6087 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2022 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
pyridoxal 5′ phosphate (PLP), vitamin B6, loss of heterozygosity (LOH), mitotic recombination, warts, Drosophila |
| جغرافية الموضوع: |
agris |
| الوصف: |
The active form of vitamin B6, pyridoxal 5′-phosphate (PLP), is a cofactor for more than 200 enzymes involved in many metabolic pathways. Moreover, PLP has antioxidant properties and quenches the reactive oxygen species (ROS). Accordingly, PLP deficiency causes chromosome aberrations in Drosophila, yeast, and human cells. In this work, we investigated whether PLP depletion can also cause loss of heterozygosity (LOH) of the tumor suppressor warts (wts) in Drosophila. LOH is usually initiated by DNA breakage in heterozygous cells for a tumor suppressor mutation and can contribute to oncogenesis inducing the loss of the wild-type allele. LOH at the wts locus results in epithelial wts homozygous tumors easily detectable on adult fly cuticle. Here, we found that PLP depletion, induced by two PLP inhibitors, promotes LOH of wts locus producing significant frequencies of wts tumors (~7% vs. 2.3%). In addition, we identified the mitotic recombination as a possible mechanism through which PLP deficiency induces LOH. Moreover, LOH of wts locus, induced by PLP inhibitors, was rescued by PLP supplementation. These data further confirm the role of PLP in genome integrity maintenance and indicate that vitamin B6 deficiency may impact on cancer also by promoting LOH. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
Molecular Genetics and Genomics; https://dx.doi.org/10.3390/ijms23116087Test |
| DOI: |
10.3390/ijms23116087 |
| الإتاحة: |
https://doi.org/10.3390/ijms23116087Test |
| حقوق: |
https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.5E9F7D78 |
| قاعدة البيانات: |
BASE |
