التفاصيل البيبلوغرافية
| العنوان: |
Recent Advances in X-Ray Hydroxyl Radical Footprinting at the Advanced Light Source Synchrotron. |
| المؤلفون: |
Morton, Simon A, Gupta, Sayan, Petzold, Christopher J, Ralston, Corie Y |
| المصدر: |
Protein and Peptide Letters, vol 26, iss 1 |
| بيانات النشر: |
eScholarship, University of California |
| سنة النشر: |
2019 |
| المجموعة: |
University of California: eScholarship |
| مصطلحات موضوعية: |
Analytical Chemistry, Chemical Sciences, Biotechnology, Crystallography, X-Ray, Fluorescent Dyes, Hydroxyl Radical, Microfluidic Analytical Techniques, Protein Conformation, Proteins, Synchrotrons, Synchrotron, footprinting, hydroxyl radical footprinting, beamline, radiolysis, radiolytic labeling, Advanced Light Source, Medicinal and Biomolecular Chemistry, Biochemistry and Cell Biology, Biophysics |
| جغرافية الموضوع: |
70 - 75 |
| الوصف: |
BackgroundSynchrotron hydroxyl radical footprinting is a relatively new structural method used to investigate structural features and conformational changes of nucleic acids and proteins in the solution state. It was originally developed at the National Synchrotron Light Source at Brookhaven National Laboratory in the late nineties, and more recently, has been established at the Advanced Light Source at Lawrence Berkeley National Laboratory. The instrumentation for this method is an active area of development, and includes methods to increase dose to the samples while implementing high-throughput sample delivery methods.ConclusionImproving instrumentation to irradiate biological samples in real time using a sample droplet generator and inline fluorescence monitoring to rapidly determine dose response curves for samples will significantly increase the range of biological problems that can be investigated using synchrotron hydroxyl radical footprinting. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
unknown |
| العلاقة: |
qt58j5879v; https://escholarship.org/uc/item/58j5879vTest |
| الإتاحة: |
https://escholarship.org/uc/item/58j5879vTest |
| حقوق: |
public |
| رقم الانضمام: |
edsbas.17A0B1ED |
| قاعدة البيانات: |
BASE |
