التفاصيل البيبلوغرافية
| العنوان: |
Increased IL-21 secretion by aged CD4+T cells is associated with prolonged STAT-4 activation and CMV seropositivity. |
| المؤلفون: |
Agrawal, Anshu, Su, Houfen, Chen, Justine, Osann, Kathryn, Agrawal, Sudhanshu, Gupta, Sudhir |
| المصدر: |
Aging, vol 4, iss 9 |
| بيانات النشر: |
eScholarship, University of California |
| سنة النشر: |
2012 |
| المجموعة: |
University of California: eScholarship |
| مصطلحات موضوعية: |
Adult, Aged, 80 and over, Aging: immunology, CD4-Positive T-Lymphocytes: cytology, immunology, metabolism, secretion, Cell Differentiation: immunology, Cells, Cultured, Coculture Techniques, Cytomegalovirus Infections: immunology, Dendritic Cells: immunology, Enzyme Activation: physiology, Enzyme-Linked Immunosorbent Assay, Female, Humans, Interleukins: secretion, Lymphocyte Activation: immunology, Male, STAT4 Transcription Factor: immunology, Young Adult |
| جغرافية الموضوع: |
648 - 659 |
| الوصف: |
Advancing age leads to significant decline in immune functions. IL-21 is produced primarily by T follicular helper (Tfh) cells and is required for effective immune cell functions. Here we compared the induction of IL-21 in aged and young subjects. Our investigation demonstrates that CD4+T cells from healthy elderly individuals (age ≥ 65) secreted significantly higher levels of IL-21 on priming with aged and young dendritic cells (DC). Though the aged and young DCs secreted comparable levels of IL-12 on stimulation with anti-CD40 antibody and LPS, culture of DCs with aged CD4+ T cells resulted in increased production of IL-21 as compared to that with young CD4+ T cells. Further examination revealed that the response of aged naïve CD4+ T cells to IL-12 was altered, resulting in increased differentiation of aged Th cells towards Tfh cells. Investigation into the signaling mechanism suggested that phosphorylation of STAT-4 in response to IL-12 was sustained for a longer duration in aged CD4+ T cells as compared to CD4+ T cells from young subjects. Additional analysis demonstrated that increased IL-21 secretion correlated with chronic CMV infection in aged subjects. These findings indicate that chronic CMV infection alters the response of aged CD4+ T cells to IL-12 resulting in an increased secretion of IL-21 and that aging affects Tfh cell responses in humans which may contribute to age-associated inflammation and immune dysfunctions. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
unknown |
| العلاقة: |
qt0mk1j4qf; https://escholarship.org/uc/item/0mk1j4qfTest |
| الإتاحة: |
https://escholarship.org/uc/item/0mk1j4qfTest |
| حقوق: |
public |
| رقم الانضمام: |
edsbas.5624A0E5 |
| قاعدة البيانات: |
BASE |
