التفاصيل البيبلوغرافية
| العنوان: |
Cas1–Cas2 complex formation mediates spacer acquisition during CRISPR–Cas adaptive immunity |
| المؤلفون: |
Nuñez, James K., Kranzusch, Philip J., Noeske, Jonas, Wright, Addison V., Davies, Christopher W., Doudna, Jennifer A. |
| المصدر: |
Nature structural & molecular biology, vol 21, iss 6 |
| بيانات النشر: |
eScholarship, University of California |
| سنة النشر: |
2014 |
| المجموعة: |
University of California: eScholarship |
| جغرافية الموضوع: |
528 - 534 |
| الوصف: |
The initial stage of CRISPR–Cas immunity involves the acquisition of foreign DNA spacer segments into the host genomic CRISPR locus. The nucleases Cas1 and Cas2 are the only proteins conserved amongst all CRISPR–Cas systems, yet the molecular functions of these proteins during immunity are unknown. Here we show that Cas1 and Cas2 from Escherichia coli form a stable complex that is essential for spacer acquisition and determine the 2.3-Å resolution crystal structure of the Cas1–Cas2 complex. Mutations that perturb Cas1–Cas2 complex formation disrupt CRISPR DNA recognition and spacer acquisition in vivo. Unlike Cas1, active site mutants of Cas2 can still acquire new spacers indicating a non-enzymatic role of Cas2 during immunity. These results reveal the universal roles of Cas1 and Cas2 and suggest a mechanism by which Cas1–Cas2 complexes specify sites of CRISPR spacer integration. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| العلاقة: |
qt5fc8631f; https://escholarship.org/uc/item/5fc8631fTest |
| الإتاحة: |
https://escholarship.org/uc/item/5fc8631fTest |
| حقوق: |
public |
| رقم الانضمام: |
edsbas.896DF3FD |
| قاعدة البيانات: |
BASE |
