دورية أكاديمية

Segregation of two endocannabinoid‐hydrolyzing enzymes into pre‐ and postsynaptic compartments in the rat hippocampus, cerebellum and amygdala

التفاصيل البيبلوغرافية
العنوان: Segregation of two endocannabinoid‐hydrolyzing enzymes into pre‐ and postsynaptic compartments in the rat hippocampus, cerebellum and amygdala
المؤلفون: Gulyas, AI, Cravatt, BF, Bracey, MH, Dinh, TP, Piomelli, D, Boscia, F, Freund, TF
المصدر: European Journal of Neuroscience, vol 20, iss 2
بيانات النشر: eScholarship, University of California
سنة النشر: 2004
المجموعة: University of California: eScholarship
مصطلحات موضوعية: Brain Disorders, Neurosciences, Substance Misuse, Drug Abuse (NIDA only), Underpinning research, 1.1 Normal biological development and functioning, Neurological, Amidohydrolases, Amygdala, Animals, Calbindin 2, Calbindins, Cerebellum, Cholecystokinin, Fluorescent Antibody Technique, Glutamate Decarboxylase, Hippocampus, Isoenzymes, Male, Mice, Knockout, Microscopy, Immunoelectron, Monoacylglycerol Lipases, Parvalbumins, Presynaptic Terminals, Rats, Wistar, S100 Calcium Binding Protein G, Synapses
جغرافية الموضوع: 441 - 458
الوصف: Fatty acid amide hydrolase (FAAH) and monoglyceride lipase (MGL) catalyse the hydrolysis of the endocannabinoids anandamide and 2-arachidonoyl glycerol. We investigated their ultrastructural distribution in brain areas where the localization and effects of cannabinoid receptor activation are known. In the hippocampus, FAAH was present in somata and dendrites of principal cells, but not in interneurons. It was located mostly on the membrane surface of intracellular organelles known to store Ca(2+) (e.g. mitochondria, smooth endoplasmic reticulum), less frequently on the somatic or dendritic plasma membrane. MGL immunoreactivity was found in axon terminals of granule cells, CA3 pyramidal cells and some interneurons. In the cerebellum, Purkinje cells and their dendrites are intensively immunoreactive for FAAH, together with a sparse axon plexus at the border of the Purkinje cell/granule cell layers. Immunostaining for MGL was complementary, the axons in the molecular layer were intensively labelled leaving the Purkinje cell dendrites blank. FAAH distribution in the amygdala was similar to that of the CB(1) cannabinoid receptor: evident signal in neuronal somata and proximal dendrites in the basolateral nucleus, and hardly any labelling in the central nucleus. MGL staining was restricted to axons in the neuropil, with similar relative signal intensities seen for FAAH in different nuclei. Thus, FAAH is primarily a postsynaptic enzyme, whereas MGL is presynaptic. FAAH is associated with membranes of cytoplasmic organelles. The differential compartmentalization of the two enzymes suggests that anandamide and 2-AG signalling may subserve functional roles that are spatially segregated at least at the stage of metabolism.
نوع الوثيقة: article in journal/newspaper
وصف الملف: application/pdf
اللغة: unknown
العلاقة: qt86z7c238; https://escholarship.org/uc/item/86z7c238Test
الإتاحة: https://escholarship.org/uc/item/86z7c238Test
حقوق: CC-BY
رقم الانضمام: edsbas.D4C040CF
قاعدة البيانات: BASE