التفاصيل البيبلوغرافية
| العنوان: |
The Type 2 Asthma Mediator IL-13 Inhibits Severe Acute Respiratory Syndrome Coronavirus 2 Infection of Bronchial Epithelium |
| المؤلفون: |
Bonser, Luke R, Eckalbar, Walter L, Rodriguez, Lauren, Shen, Jiangshan, Koh, Kyung Duk, Ghias, Khadija, Zlock, Lorna T, Christenson, Stephanie, Woodruff, Prescott G, Finkbeiner, Walter E, Erle, David J |
| المصدر: |
American Journal of Respiratory Cell and Molecular Biology, vol 66, iss 4 |
| بيانات النشر: |
eScholarship, University of California |
| سنة النشر: |
2022 |
| المجموعة: |
University of California: eScholarship |
| مصطلحات موضوعية: |
Medical Microbiology, Bioinformatics and Computational Biology, Biomedical and Clinical Sciences, Biological Sciences, Prevention, Pneumonia & Influenza, Lung, Biotechnology, Genetics, Infectious Diseases, Asthma, Emerging Infectious Diseases, Pneumonia, Aetiology, 2.1 Biological and endogenous factors, Infection, Respiratory, Good Health and Well Being, COVID-19, Cells, Cultured, Epithelial Cells, Epithelium, Humans, Interleukin-13, SARS-CoV-2, IL-13, airway epithelium, Cardiorespiratory Medicine and Haematology, Respiratory System |
| جغرافية الموضوع: |
391 - 401 |
| الوصف: |
Asthma is associated with chronic changes in the airway epithelium, a key target of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Many epithelial changes, including goblet cell metaplasia, are driven by the type 2 cytokine IL-13, but the effects of IL-13 on SARS-CoV-2 infection are unknown. We found that IL-13 stimulation of differentiated human bronchial epithelial cells (HBECs) cultured at air-liquid interface reduced viral RNA recovered from SARS-CoV-2-infected cells and decreased double-stranded RNA, a marker of viral replication, to below the limit of detection in our assay. An intact mucus gel reduced SARS-CoV-2 infection of unstimulated cells, but neither a mucus gel nor SPDEF, which is required for goblet cell metaplasia, were required for the antiviral effects of IL-13. Bulk RNA sequencing revealed that IL-13 regulated 41 of 332 (12%) mRNAs encoding SARS-CoV-2-associated proteins that were detected in HBECs (>1.5-fold change; false discovery rate < 0.05). Although both IL-13 and IFN-α each inhibit SARS-CoV-2 infection, their transcriptional effects differed markedly. Single-cell RNA sequencing revealed cell type-specific differences in SARS-CoV-2-associated gene expression and IL-13 responses. Many IL-13-induced gene expression changes were seen in airway epithelium from individuals with type 2 asthma and chronic obstructive pulmonary disease. IL-13 effects on airway epithelial cells may protect individuals with type 2 asthma from COVID-19 and could lead to identification of novel strategies for reducing SARS-CoV-2 infection. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
unknown |
| العلاقة: |
qt7rw881r4; https://escholarship.org/uc/item/7rw881r4Test |
| الإتاحة: |
https://escholarship.org/uc/item/7rw881r4Test |
| حقوق: |
public |
| رقم الانضمام: |
edsbas.7DF48393 |
| قاعدة البيانات: |
BASE |
