التفاصيل البيبلوغرافية
| العنوان: |
High-throughput sequencing of two populations of extracellular vesicles provides an mRNA signature that can be detected in the circulation of breast cancer patients |
| المؤلفون: |
Conley, Andrew, Minciacchi, Valentina R, Lee, Dhong Hyun, Knudsen, Beatrice S, Karlan, Beth Y, Citrigno, Luigi, Viglietto, Giuseppe, Tewari, Muneesh, Freeman, Michael R, Demichelis, Francesca, Di Vizio, Dolores |
| المصدر: |
RNA Biology, vol 14, iss 3 |
| بيانات النشر: |
eScholarship, University of California |
| سنة النشر: |
2017 |
| المجموعة: |
University of California: eScholarship |
| مصطلحات موضوعية: |
Biological Sciences, Bioinformatics and Computational Biology, Genetics, Clinical Research, Breast Cancer, Cancer, Aetiology, 2.1 Biological and endogenous factors, Biomarkers, Tumor, Breast Neoplasms, Cell Cycle, Cell Line, Computational Biology, Cytosol, E2F4 Transcription Factor, Extracellular Vesicles, Female, Gene Expression Profiling, Gene Ontology, High-Throughput Nucleotide Sequencing, Humans, Protein Sorting Signals, RNA, Messenger, Transcriptome, Vascular Endothelial Growth Factor A, Developmental Biology, Biochemistry and cell biology |
| جغرافية الموضوع: |
305 - 316 |
| الوصف: |
Extracellular vesicles (EVs) contain a wide range of RNA types with a reported prevalence of non-coding RNA. To date a comprehensive characterization of the protein coding transcripts in EVs is still lacking. We performed RNA-Sequencing (RNA-Seq) of 2 EV populations and identified a small fraction of transcripts that were expressed at significantly different levels in large oncosomes and exosomes, suggesting they may mediate specialized functions. However, these 2 EV populations exhibited a common mRNA signature that, in comparison to their donor cells, was significantly enriched in mRNAs encoding E2F transcriptional targets and histone proteins. These mRNAs are primarily expressed in the S-phase of the cell cycle, suggesting that they may be packaged into EVs during S-phase. In silico analysis using subcellular compartment transcriptome data from the ENCODE cell line compendium revealed that EV mRNAs originate from a cytoplasmic RNA pool. The EV signature was independently identified in plasma of patients with breast cancer by RNA-Seq. Furthermore, several transcripts differentially expressed in EVs from patients versus controls mirrored differential expression between normal and breast cancer tissues. Altogether, this largest high-throughput profiling of EV mRNA demonstrates that EVs carry tumor-specific alterations and can be interrogated as a source of cancer-derived cargo. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
unknown |
| العلاقة: |
qt0sz8j8jm; https://escholarship.org/uc/item/0sz8j8jmTest |
| الإتاحة: |
https://escholarship.org/uc/item/0sz8j8jmTest |
| حقوق: |
public |
| رقم الانضمام: |
edsbas.93A11F4F |
| قاعدة البيانات: |
BASE |
