التفاصيل البيبلوغرافية
| العنوان: |
Phosphoinositide-mediated clathrin adaptor progression at the trans-Golgi network |
| المؤلفون: |
Daboussi, Lydia, Costaguta, Giancarlo, Payne, Gregory S |
| المصدر: |
Nature Cell Biology, vol 14, iss 3 |
| بيانات النشر: |
eScholarship, University of California |
| سنة النشر: |
2012 |
| المجموعة: |
University of California: eScholarship |
| مصطلحات موضوعية: |
Underpinning research, 1.1 Normal biological development and functioning, Generic health relevance, 1-Phosphatidylinositol 4-Kinase, Adaptor Protein Complex 1, Adaptor Proteins, Vesicular Transport, Clathrin, Clathrin-Coated Vesicles, Endosomes, Immunoblotting, Luminescent Proteins, Microscopy, Confocal, Fluorescence, Mutation, Phosphatidylinositol Phosphates, Protein Binding, Protein Transport, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Time-Lapse Imaging, trans-Golgi Network, Biological Sciences, Medical and Health Sciences, Developmental Biology |
| جغرافية الموضوع: |
239 - 248 |
| الوصف: |
Clathrin-coated vesicles mediate endocytosis and transport between the trans-Golgi network (TGN) and endosomes in eukaryotic cells. Clathrin adaptors play central roles in coat assembly, interacting with clathrin, cargo and membranes. Two main types of clathrin adaptor act in TGN-endosome traffic: GGA proteins and the AP-1 complex. Here we characterize the relationship between GGA proteins, AP-1 and other TGN clathrin adaptors using live-cell and super-resolution microscopy in yeast. We present evidence that GGA proteins and AP-1 are recruited sequentially in two waves of coat assembly at the TGN. Mutations that decrease phosphatidylinositol 4-phosphate (PtdIns(4)P) levels at the TGN slow or uncouple AP-1 coat assembly from GGA coat assembly. Conversely, enhanced PtdIns(4)P synthesis shortens the time between adaptor waves. Gga2p binds directly to the TGN PtdIns(4)-kinase Pik1p and contributes to Pik1p recruitment. These results identify a PtdIns(4)P-based mechanism for regulating progressive assembly of adaptor-specific clathrin coats at the TGN. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
unknown |
| العلاقة: |
qt0vf8v0b6; https://escholarship.org/uc/item/0vf8v0b6Test |
| الإتاحة: |
https://escholarship.org/uc/item/0vf8v0b6Test |
| حقوق: |
public |
| رقم الانضمام: |
edsbas.953149E3 |
| قاعدة البيانات: |
BASE |
