التفاصيل البيبلوغرافية
| العنوان: |
Retinal degeneration in two lines of transgenic S334ter rats |
| المؤلفون: |
Martinez-Navarrete, G, Seiler, MJ, Aramant, RB, Fernandez-Sanchez, L, Pinilla, I, Cuenca, N |
| المصدر: |
Experimental Eye Research, vol 92, iss 3 |
| بيانات النشر: |
eScholarship, University of California |
| سنة النشر: |
2011 |
| المجموعة: |
University of California: eScholarship |
| مصطلحات موضوعية: |
Neurosciences, Eye Disease and Disorders of Vision, Amacrine Cells, Animals, Apoptosis, Calbindins, Disease Models, Animal, Female, Fluorescent Antibody Technique, Indirect, In Situ Nick-End Labeling, Male, Parvalbumins, Photoreceptor Cells, Vertebrate, Presynaptic Terminals, Protein Kinase C, Rats, Long-Evans, Sprague-Dawley, Transgenic, Recoverin, Retinal Bipolar Cells, Retinal Degeneration, Retinal Horizontal Cells, S100 Calcium Binding Protein G, Transducin, rodent, remodeling |
| جغرافية الموضوع: |
227 - 237 |
| الوصف: |
Aim of this study was to examine synaptic connectivity changes in the retina and the location and rate of apoptosis in transgenic S334ter line-3 and line-5 rats with photoreceptor degeneration. Heterozygous S334ter-line-3 and line-5 at P11-13, P30, P60, P90 and several control non-dystrophic rats (Long Evans and Sprague-Dawley) at P60, were studied anatomically by immunohistochemistry for various cell and synaptic markers, and by PNA and TUNEL label.- S334ter line-3 exhibited the fastest rate of degeneration with an early loss of photoreceptors, with 1-2 layers remaining at P30, and only cones left at P60. Line-5 had 4-5 layers left at P30, and very few rods left at P60-90. In both lines, horizontal cell processes (including dendrites and axon) were diminished at P11-13, showing gaps in the outer plexiform layer (OPL) at P60, and at P90, almost no terminal tips could be seen. Bipolar cells showed a retraction of their dendrites forming clusters along the OPL. Synaptic terminals of A-II amacrine cells in the IPL lost most of their parvalbumin-immunoreactivity. The apoptosis rate was different in both lines. Line-3 rats showed many photoreceptors affected at P11, occupying the innermost part of the outer nuclear layer. Line-5 showed a lower number of apoptotic cells within the same location at P13. In summary, the S334ter line-3 rat has a faster progression of degeneration than line-5. The horizontal and bipolar terminals are already affected at P11-P13 in both models. Apoptosis is related to the mutated rhodopsin transgene; the first photoreceptor cells affected are those close to the OPL. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
unknown |
| العلاقة: |
qt8jb105k2; https://escholarship.org/uc/item/8jb105k2Test |
| الإتاحة: |
https://escholarship.org/uc/item/8jb105k2Test |
| حقوق: |
public |
| رقم الانضمام: |
edsbas.1E303ED1 |
| قاعدة البيانات: |
BASE |
