دورية أكاديمية
Bioprinted 3D Bionic Scaffolds with Pancreatic Islets as a New Therapy for Type 1 Diabetes—Analysis of the Results of Preclinical Studies on a Mouse Model
| العنوان: | Bioprinted 3D Bionic Scaffolds with Pancreatic Islets as a New Therapy for Type 1 Diabetes—Analysis of the Results of Preclinical Studies on a Mouse Model |
|---|---|
| المؤلفون: | Marta Klak, Michał Wszoła, Andrzej Berman, Anna Filip, Anna Kosowska, Joanna Olkowska-Truchanowicz, Michał Rachalewski, Grzegorz Tymicki, Tomasz Bryniarski, Marta Kołodziejska, Tomasz Dobrzański, Dominika Ujazdowska, Jarosław Wejman, Izabela Uhrynowska-Tyszkiewicz, Artur Kamiński |
| المصدر: | Journal of Functional Biomaterials, Vol 14, Iss 7, p 371 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biotechnology LCC:Medicine (General) |
| مصطلحات موضوعية: | diabetes, pancreas islets, 3D bioprinting, glucose, xenotransplantation, bionic scaffold, Biotechnology, TP248.13-248.65, Medicine (General), R5-920 |
| الوصف: | Recently, tissue engineering, including 3D bioprinting of the pancreas, has acquired clinical significance and has become an outstanding potential method of customized treatment for type 1 diabetes mellitus. The study aimed to evaluate the function of 3D-bioprinted pancreatic petals with pancreatic islets in the murine model. A total of 60 NOD-SCID (Nonobese diabetic/severe combined immunodeficiency) mice were used in the study and divided into three groups: control group; IsletTx (porcine islets transplanted under the renal capsule); and 3D bioprint (3D-bioprinted pancreatic petals with islets transplanted under the skin, on dorsal muscles). Glucose, C-peptide concentrations, and histological analyses were performed. In the obtained results, significantly lower mean fasting glucose levels (mg/dL) were observed both in a 3D-bioprint group and in a group with islets transplanted under the renal capsule when compared with untreated animals. Differences were observed in all control points: 7th, 14th, and 28th days post-transplantation (129, 119, 118 vs. 140, 139, 140; p < 0.001). Glucose levels were lower on the 14th and 28th days in a group with bioprinted petals compared to the group with islets transplanted under the renal capsule. Immunohistochemical staining indicated the presence of secreted insulin-living pancreatic islets and neovascularization within 3D-bioprinted pancreatic petals after transplantation. In conclusion, bioprinted bionic petals significantly lowered plasma glucose concentration in studied model species. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2079-4983 |
| العلاقة: | https://www.mdpi.com/2079-4983/14/7/371Test; https://doaj.org/toc/2079-4983Test |
| DOI: | 10.3390/jfb14070371 |
| الوصول الحر: | https://doaj.org/article/1facc1131e754933ac40678fe286932cTest |
| رقم الانضمام: | edsdoj.1facc1131e754933ac40678fe286932c |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20794983 |
|---|---|
| DOI: | 10.3390/jfb14070371 |