دورية أكاديمية
Successful use of whole genome amplified DNA from multiple source types for high-density Illumina SNP microarrays
| العنوان: | Successful use of whole genome amplified DNA from multiple source types for high-density Illumina SNP microarrays |
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| المؤلفون: | Casey L. Dagnall, Lindsay M. Morton, Belynda D. Hicks, Shengchao Li, Weiyin Zhou, Eric Karlins, Kedest Teshome, Salma Chowdhury, Kerrie S. Lashley, Joshua N. Sampson, Leslie L. Robison, Gregory T. Armstrong, Smita Bhatia, Gretchen A. Radloff, Stella M. Davies, Margaret A. Tucker, Meredith Yeager, Stephen J. Chanock |
| المصدر: | BMC Genomics, Vol 19, Iss 1, Pp 1-10 (2018) |
| بيانات النشر: | BMC, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Biotechnology LCC:Genetics |
| مصطلحات موضوعية: | Whole genome amplification, Genome-wide association study, High-density microarray, CCSS clinical trial, Biotechnology, TP248.13-248.65, Genetics, QH426-470 |
| الوصف: | Abstract Background The recommended genomic DNA input requirements for whole genome single nucleotide polymorphism microarrays can limit the scope of molecular epidemiological studies. We performed a large-scale evaluation of whole genome amplified DNA as input into high-density, whole-genome Illumina® Infinium® SNP microarray. Results Overall, 6622 DNA samples from 5970 individuals were obtained from three distinct biospecimen sources and genotyped using gDNA and/or wgaDNA inputs. When genotypes from the same individual were compared with standard, native gDNA input amount, we observed 99.94% mean concordance with wgaDNA input. Conclusions Our results demonstrate that carefully conducted studies with wgaDNA inputs can yield high-quality genotyping results. These findings should enable investigators to consider expansion of ongoing studies using high-density SNP microarrays, currently challenged by small amounts of available DNA. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1471-2164 |
| العلاقة: | http://link.springer.com/article/10.1186/s12864-018-4572-6Test; https://doaj.org/toc/1471-2164Test |
| DOI: | 10.1186/s12864-018-4572-6 |
| الوصول الحر: | https://doaj.org/article/371d8d142e9f4e97baedf052bf07d0acTest |
| رقم الانضمام: | edsdoj.371d8d142e9f4e97baedf052bf07d0ac |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14712164 |
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| DOI: | 10.1186/s12864-018-4572-6 |