دورية أكاديمية
Effect of water availability on changes in root amino acids and associated rhizosphere on root exudation of amino acids in Pisum sativum L.
| العنوان: | Effect of water availability on changes in root amino acids and associated rhizosphere on root exudation of amino acids in Pisum sativum L. |
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| المؤلفون: | Bobille, Helene, Fustec, Joelle, Robins, Richard J., Cukier, Caroline, Limami, Anis M. |
| المساهمون: | Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Légumineuses, Ecophysiologie Végétale, Agroécologie (LEVA), Institut National de la Recherche Agronomique (INRA)-Ecole supérieure d'Agricultures d'Angers (ESA), Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), French Region Pays-de-la-Loire, project "RHIZOSFER" (2012-09695 ), French Region Pays-de-la-Loire, project "PLAISIR" Association for International Structure on "Isotopomic Research" French Regional International Strategy Grant |
| المصدر: | Phytochemistry ; https://hal.science/hal-02140854Test ; Phytochemistry, 2019, 161, pp.75-85. ⟨10.1016/j.phytochem.2019.01.015⟩ |
| بيانات النشر: | HAL CCSD |
| سنة النشر: | 2019 |
| المجموعة: | Université de Nantes: HAL-UNIV-NANTES |
| مصطلحات موضوعية: | Leguminoseae, Amino acids, Water stress, CG-MS, 15N-labelling, [CHIM]Chemical Sciences |
| الوصف: | International audience ; Root exudation is considered to regulate the abundance of the microbial community. It may vary both qualitatively and quantitatively in response to the environment in which the plant is growing. A part of exuded N derives from amino acids (AAs). This, in turn, may help plants to cope with abiotic stresses by favouring positive interactions with the rhizosphere environment, thus playing a potential role in maintaining healthy plants. In this respect, an under-investigated area is the effect of stress due to water deficit (WD). It is proposed that the AA profile in the rhizosphere may be altered by WD, reflecting a modulation of root AA exudation linked to a physiological response of the plant to water stress. To investigate this, Pisum sativum L. plants, grown in unsterilised Rhizobium leguminosarum-enriched soil, were stem-labelled with N-15-urea for 96 h, and then subjected/not subjected to 72 h of WD. The concentrations and abundance of N-15-labelling in individual AAs were determined in both roots and the associated rhizosphere at 24,48 and 72 h after stress application. It was found that both AAs metabolism in the pea root and AAs exudation were strongly modified in WD conditions. After 24 h of WD, the concentrations of all measured AAs increased in the roots, accompanied by a dramatic stress-related increase in the N-15-labelling of some AAs. Furthermore, after 48-72 h of WD, the concentrations of Pro, Ala and Glu increased significantly within the rhizosphere, notably with a concomitant increase in N-15-enrichment in Pro, Ser, Asn, Asp, Thr and Ile. These results support the concept that, in response to WD, substantial amounts of recently assimilated N are rapidly translocated from the shoots to the roots, a portion of which is exuded as AAs. This leads to the rhizosphere being relatively augmented by specific AAs (notably HSer, Pro and Ala) in WD conditions, with a potential impact on soil water retention. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/30822623; hal-02140854; https://hal.science/hal-02140854Test; https://hal.science/hal-02140854/documentTest; https://hal.science/hal-02140854/file/S0031942218302334.pdfTest; PII: S0031-9422(18)30233-4; PRODINRA: 478073; PUBMED: 30822623; WOS: 000465062600006 |
| DOI: | 10.1016/j.phytochem.2019.01.015 |
| الإتاحة: | https://doi.org/10.1016/j.phytochem.2019.01.015Test https://hal.science/hal-02140854Test https://hal.science/hal-02140854/documentTest https://hal.science/hal-02140854/file/S0031942218302334.pdfTest |
| حقوق: | http://creativecommons.org/licenses/by-ncTest/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.6704FD8B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.phytochem.2019.01.015 |
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