Construction of an adenovirus type 7a E1A- vector
| العنوان: | Construction of an adenovirus type 7a E1A- vector |
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| المؤلفون: | Karil Abrahamsen, D Brough, H L Kong, A Lizonova, Andrea Mastrangeli, Erik Falck-Pedersen, Ronald G. Crystal |
| المصدر: | Journal of Virology. 71:8946-8951 |
| بيانات النشر: | American Society for Microbiology, 1997. |
| سنة النشر: | 1997 |
| مصطلحات موضوعية: | Gene Expression Regulation, Viral, viruses, Transgene, Genetic Vectors, Immunology, Biology, Virus Replication, Microbiology, Virus, Defective virus, Chloramphenicol acetyltransferase, Mice, Plasmid, Transduction, Genetic, Virology, Animals, Humans, Vector (molecular biology), Cells, Cultured, Adenovirus genome, Adenoviruses, Human, Gene Transfer Techniques, Defective Viruses, Molecular biology, Viral replication, Insect Science, Adenovirus E1A Proteins, Research Article |
| الوصف: | A strategy for constructing replication-defective adenovirus vectors from non-subgroup C viruses has been successfully demonstrated with adenovirus type 7 strain a (Ad7a) as the prototype. An E1A-deleted Ad7a reporter virus expressing the chloramphenicol acetyltransferase (CAT) gene from the cytomegalovirus promoter enhancer was constructed with DNA fragments isolated from Ad7a, an Ad7a recombination reporter plasmid, and the 293 cell line. The Ad7a-CAT virus particle transduces A549 cells as efficiently as Ad5-based vectors. Intravenous infections in a murine model indicate that the Ad7a-CAT virus infects a variety of tissues, with maximal levels of CAT gene expression found in the liver. The duration of Ad7a-CAT transgene expression in the liver was maximally maintained 2 weeks postinfection, with a decline to baseline activity by the week 4 postinfection. Ad7a-CAT represents the first example of a non-subgroup C E1A- adenovirus gene transfer vector. |
| تدمد: | 1098-5514 0022-538X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c7be7bad30c94b03c10fe8431b60e38Test https://doi.org/10.1128/jvi.71.11.8946-8951.1997Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1c7be7bad30c94b03c10fe8431b60e38 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985514 0022538X |
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