Intensity distribution segmentation in ultrafast Doppler combined with scanning laser confocal microscopy for assessing vascular changes associated with ageing in murine hippocampi
| العنوان: | Intensity distribution segmentation in ultrafast Doppler combined with scanning laser confocal microscopy for assessing vascular changes associated with ageing in murine hippocampi |
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| المؤلفون: | Maximiliano Anzibar Fialho, Lucia Vázquez Alberdi, Mariana Martínez, Miguel Calero, Jerome Baranger, Mickael Tanter, Juan Pablo Damián, Carlos Negreira, Nicolás Rubido, Alejandra Kun, Javier Brum |
| المساهمون: | Agencia Nacional de Investigación e Innovación (Uruguay) |
| المصدر: | Repisalud Instituto de Salud Carlos III (ISCIII) |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Aging, Multidisciplinary, Microscopy, Confocal, Lasers, Imaging techniques, Neural ageing, behavioral disciplines and activities, Hippocampus, Confocal microscopy, Ageing, Mice, Cross-Sectional Studies, Ultrasound, Animals, Longitudinal Studies, psychological phenomena and processes |
| الوصف: | Publisher Correction: Intensity distribution segmentation in ultrafast Doppler combined with scanning laser confocal microscopy for assessing vascular changes associated with ageing in murine hippocampi PMID: 35538217 https://doi.org/10.1038/s41598-022-11822-4Test The hippocampus plays an important role in learning and memory, requiring high-neuronal oxygenation. Understanding the relationship between blood flow and vascular structure-and how it changes with ageing-is physiologically and anatomically relevant. Ultrafast Doppler ([Formula: see text]Doppler) and scanning laser confocal microscopy (SLCM) are powerful imaging modalities that can measure in vivo cerebral blood volume (CBV) and post mortem vascular structure, respectively. Here, we apply both imaging modalities to a cross-sectional and longitudinal study of hippocampi vasculature in wild-type mice brains. We introduce a segmentation of CBV distribution obtained from [Formula: see text]Doppler and show that this mice-independent and mesoscopic measurement is correlated with vessel volume fraction (VVF) distribution obtained from SLCM-e.g., high CBV relates to specific vessel locations with large VVF. Moreover, we find significant changes in CBV distribution and vasculature due to ageing (5 vs. 21 month-old mice), highlighting the sensitivity of our approach. Overall, we are able to associate CBV with vascular structure-and track its longitudinal changes-at the artery-vein, venules, arteriole, and capillary levels. We believe that this combined approach can be a powerful tool for studying other acute (e.g., brain injuries), progressive (e.g., neurodegeneration) or induced pathological changes. This work was mainly funded by the Agencia Nacional de Investigación e Innovación (ANII), grant FCE_1_2019_1_155539. The authors also thank the support of PEDECIBA, CSIC-UdelaR and the Institut Franco—Uruguayen de Physique (IFUP), LIA-CNRS-UdelaR. J.P.D., C.N., N.R., A.K. and J.Br. thank SNI-ANII. M.A.F. thanks the support of ANII through POS_NAC_M_2020_1_164127 scholarship. M.M. thanks the support of ANII through POS_FCE_2020_1_1009181 scholarship. N.R. thanks the support of CSIC I+D group grant CSIC2018—FID 13—Grupo ID 722. Sí |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a99035fd998531a38e6a53d87ab69a1Test https://pubmed.ncbi.nlm.nih.gov/35538217Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7a99035fd998531a38e6a53d87ab69a1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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