دورية أكاديمية
Saponins from bitter melon reduce lipid accumulation via induction of autophagy in C. elegans and HepG2 cell line
| العنوان: | Saponins from bitter melon reduce lipid accumulation via induction of autophagy in C. elegans and HepG2 cell line |
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| المؤلفون: | Juan Bai, Ying Zhu, Linzhao He, Jinfu Zhang, Jie Li, Ruirong Pan, Jiayan Zhang, Yansheng Zhao, Lin Cui, Haina Lu, Ya Jiang, Xiang Xiao |
| المصدر: | Current Research in Food Science, Vol 5, Iss , Pp 1167-1175 (2022) |
| بيانات النشر: | Elsevier, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Nutrition. Foods and food supply LCC:Food processing and manufacture |
| مصطلحات موضوعية: | Bitter melon saponin, Fat accumulation, Lipidomics, Autophagy, Daf-16/ hlh-30, Nutrition. Foods and food supply, TX341-641, Food processing and manufacture, TP368-456 |
| الوصف: | Saponins from bitter melon (BMS) are well-known to have various biological activities, especially in the field of fat-lowering. However, many gaps remain in our knowledge of BMS-induced fat reduction and health benefits. Here, we aimed to investigate the precise mechanism of BMS in alleviating fat accumulation in C. elegans and HepG2 cell line. Results indicated that BMS showed strong fat-lowering and lifespan-extension properties. Lipidomic analysis illustrated that BMS could alter the lipid profile, especially represented by phosphatidylethanolamine (PE) increase, which plays an essential role in autophagy. Furthermore, we applied gene-deficient mutants and RNAi technology to confirm that BMS largely depended on daf-16/FoxO1 and hlh-30/TFEB mediated lipophagy to reduce fat deposition. In addition, BMS could ameliorate oil acid (OA)-induced fat accumulation in HepG2 cells by induction of autophagy-related proteins, such as the phosphorylated AMPK and LC3B. In conclusion, our results elucidated the underlying mechanism of bitter melon saponins interfering with lipid metabolism from the autophagy point of view, which provide new insights into a nutraceutical to mitigate obesity. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2665-9271 |
| العلاقة: | http://www.sciencedirect.com/science/article/pii/S2665927122001009Test; https://doaj.org/toc/2665-9271Test |
| DOI: | 10.1016/j.crfs.2022.06.011 |
| الوصول الحر: | https://doaj.org/article/4455cb52608940e786270c607612b090Test |
| رقم الانضمام: | edsdoj.4455cb52608940e786270c607612b090 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 26659271 |
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| DOI: | 10.1016/j.crfs.2022.06.011 |