التفاصيل البيبلوغرافية
| العنوان: |
A nonerythropoietic derivative of erythropoietin inhibits tubulointerstitial fibrosis in remnant kidney. |
| المؤلفون: |
Imamura, Ryoichi, Isaka, Yoshitaka, Sandoval, Ruben, Ichimaru, Naotsugu, Abe, Toyofumi, Okumi, Masayoshi, Yazawa, Koji, Kitamura, Harumi, Kaimori, Jyunya, Nonomura, Norio, Rakugi, Hiromi, Molitoris, Bruce, Takahara, Shiro |
| المصدر: |
Clinical & Experimental Nephrology; Dec2012, Vol. 16 Issue 6, p852-862, 11p |
| مصطلحات موضوعية: |
ERYTHROPOIETIN, INTERSTITIAL nephritis, FIBROSIS, KIDNEY diseases, DRUG administration, HEMOGLOBINS, APOPTOSIS, TRANSFORMING growth factors |
| مستخلص: |
Background: The tissue-protective effects of erythropoietin (EPO) have been extensively investigated, and EPO administration can raise the hemoglobin (Hb) concentration. Recently, we reported that carbamylated erythropoietin (CEPO) protected kidneys from ischemia-reperfusion injury as well as EPO. Methods: To investigate the clinical applications of CEPO, we next evaluated the long-term therapeutic effect of CEPO using a tubulointerstitial model rat. We randomized remnant kidney model rats to receive saline, EPO, or CEPO for 8 weeks. Results: CEPO- and EPO-treated rats had improved serum creatinine levels compared with saline-treated remnant kidney model rats, although the Hb level was significantly increased in EPO-treated rats. Two-photon microscopy revealed that EPO/CEPO significantly ameliorated tubular epithelial cell damage assessed by endocytosis. In addition, CEPO or EPO protected endothelial cells with a sustained blood flow rate. EPO or CEPO suppressed the number of TUNEL-positive apoptotic cells with weak αSMA staining. Furthermore, PCR analysis demonstrated that TGF-β and type I collagen expression was attenuated in EPO- or CEPO-treated rats, accompanied by a significant decrease in interstitial fibrosis. Conclusion: We established a long-term therapeutic approach to protect tubulointerstitial injury with CEPO, and thus, the therapeutic value of this approach warrants further attention and preclinical studies. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
