دورية أكاديمية
Intestinal metaplasia of the sinonasal mucosa adjacent to intestinal-type adenocarcinoma. A morphologic, immunohistochemical, and molecular study
| العنوان: | Intestinal metaplasia of the sinonasal mucosa adjacent to intestinal-type adenocarcinoma. A morphologic, immunohistochemical, and molecular study |
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| المؤلفون: | FRANCHI, ALESSANDRO, Palomba, Annarita, Miligi, Lucia, Ranucci, Valentina, Innocenti, Duccio Rossi Degli, Simoni, Antonella, Pepi, Monica, Santucci, Marco |
| المساهمون: | Franchi, Alessandro, Palomba, Annarita, Miligi, Lucia, Ranucci, Valentina, Innocenti, Duccio Rossi Degli, Simoni, Antonella, Pepi, Monica, Santucci, Marco |
| سنة النشر: | 2015 |
| المجموعة: | ARPI - Archivio della Ricerca dell'Università di Pisa |
| مصطلحات موضوعية: | Immunohistochemistry, Intestinal metaplasia, Intestinal-type adenocarcinoma, Nasal cavitie, Paranasal sinuse, TP53 gene, Adenocarcinoma, Aged, 80 and over, Biomarkers, Tumor, Human, Intestine, Male, Metaplasia, Middle Aged, Nasal Mucosa, Paranasal Sinus Neoplasm, Reverse Transcriptase Polymerase Chain Reaction, Cell Biology, Molecular Biology, Medicine (all) |
| الوقت: | 2734 |
| الوصف: | It has been hypothesized that the development of sinonasal intestinal-type adenocarcinoma (ITAC) occurs through intestinal metaplasia (IM) of the respiratory and/or glandular epithelium. The aim of this study was to characterize the histological, immunohistochemical, and molecular features of sinonasal IM. Histologic slides from 29 consecutive surgical specimens of ITAC were retrieved. Sections were stained for CDX2, cytokeratin 20 (CK20), MUC2, and p53. The status of TP53 gene exons 4–9 was assessed separately in areas of IM and in ITAC. Foci of IM were detected in eight cases (27.5Â%). They were all positive for CK20 and CDX2, while MUC2 was detected in six cases (75Â%). In six cases (75Â%), the metaplastic foci showed signs of dysplasia, including nuclear enlargement with increased nucleus to cytoplasm ratio, nuclear hyperchromasia, loss of nuclear polarity, and presence of prominent nucleoli. P53 nuclear immunoreactivity was observed in four cases. TP53 gene sequencing was successfully performed in six cases and revealed the same mutation in both IM and ITAC in two cases (c.832C > T and c.215G > C), while another ITAC showed a mutation that was not present in the adjacent IM (c.536A > G). In conclusion, our study suggests a possible clonal relationship between areas of sinonasal IM and ITAC, indicating that IM may represent a precursor lesion of ITAC. Improving the knowledge on the morphological and molecular features of IM is a key step to identify reliable biomarkers to determine the risk of sinonasal ITAC development. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | STAMPA |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/25431194; info:eu-repo/semantics/altIdentifier/wos/WOS:000349377800006; volume:466; issue:2; firstpage:161; lastpage:168; numberofpages:8; journal:VIRCHOWS ARCHIV; http://hdl.handle.net/11568/876385Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84925506873; link.springer.de/link/service/journals/00428/index.htm |
| DOI: | 10.1007/s00428-014-1696-1 |
| الإتاحة: | https://doi.org/10.1007/s00428-014-1696-1Test http://hdl.handle.net/11568/876385Test |
| رقم الانضمام: | edsbas.E6EB376A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/s00428-014-1696-1 |
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