المؤلفون: Yuyao Gao, Yanli Yao, Xin Chen, Jianyang Wu, Qingsong Wu, Shenghui Liu, Anping Guo, Xiumei Zhang

المصدر: Frontiers in Plant Science, Vol 13 (2022)

مصطلحات موضوعية: Ananas comosus, fruit quality, sucrose, citric acid, metabolic genes, transporter genes, Plant culture, SB1-1110

الوصف: Pineapple (Ananas comosus L.) is one of the most valuable subtropical fruit crop in the world. The sweet-acidic taste of the pineapple fruits is a major contributor to the characteristic of fruit quality, but its formation mechanism remains elusive. Here, targeted metabolomic and transcriptomic analyses were performed during the fruit developmental stages in two pineapple cultivars (“Comte de Paris” and “MD-2”) to gain a global view of the metabolism and transport pathways involved in sugar and organic acid accumulation. Assessment of the levels of different sugar and acid components during fruit development revealed that the predominant sugar and organic acid in mature fruits of both cultivars was sucrose and citric acid, respectively. Weighted gene coexpression network analysis of metabolic phenotypes and gene expression profiling enabled the identification of 21 genes associated with sucrose accumulation and 19 genes associated with citric acid accumulation. The coordinated interaction of the 21 genes correlated with sucrose irreversible hydrolysis, resynthesis, and transport could be responsible for sucrose accumulation in pineapple fruit. In addition, citric acid accumulation might be controlled by the coordinated interaction of the pyruvate-to-acetyl-CoA-to-citrate pathway, gamma-aminobutyric acid pathway, and tonoplast proton pumps in pineapple. These results provide deep insights into the metabolic regulation of sweetness and acidity in pineapple.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fpls.2022.971506/fullTest; https://doaj.org/toc/1664-462XTest

الوصول الحر: https://doaj.org/article/3155e7fc9b9b414e9541fcc28fdebadaTest

المؤلفون: Yuyao Gao, Yanli Yao, Xin Chen, Jianyang Wu, Qingsong Wu, Shenghui Liu, Anping Guo, Xiumei Zhang

مصطلحات موضوعية: Botany, Plant Biology, Plant Systematics and Taxonomy, Plant Cell and Molecular Biology, Plant Developmental and Reproductive Biology, Plant Pathology, Plant Physiology, Plant Biology not elsewhere classified, Ananas comosus, fruit quality, sucrose, citric acid, metabolic genes, transporter genes

الوصف: Pineapple (Ananas comosus L.) is one of the most valuable subtropical fruit crop in the world. The sweet-acidic taste of the pineapple fruits is a major contributor to the characteristic of fruit quality, but its formation mechanism remains elusive. Here, targeted metabolomic and transcriptomic analyses were performed during the fruit developmental stages in two pineapple cultivars (“Comte de Paris” and “MD-2”) to gain a global view of the metabolism and transport pathways involved in sugar and organic acid accumulation. Assessment of the levels of different sugar and acid components during fruit development revealed that the predominant sugar and organic acid in mature fruits of both cultivars was sucrose and citric acid, respectively. Weighted gene coexpression network analysis of metabolic phenotypes and gene expression profiling enabled the identification of 21 genes associated with sucrose accumulation and 19 genes associated with citric acid accumulation. The coordinated interaction of the 21 genes correlated with sucrose irreversible hydrolysis, resynthesis, and transport could be responsible for sucrose accumulation in pineapple fruit. In addition, citric acid accumulation might be controlled by the coordinated interaction of the pyruvate-to-acetyl-CoA-to-citrate pathway, gamma-aminobutyric acid pathway, and tonoplast proton pumps in pineapple. These results provide deep insights into the metabolic regulation of sweetness and acidity in pineapple.

العلاقة: https://figshare.com/articles/dataset/Table_1_Metabolomic_and_transcriptomic_analyses_reveal_the_mechanism_of_sweet-acidic_taste_formation_during_pineapple_fruit_development_XLSX/21061438Test

الإتاحة: https://doi.org/10.3389/fpls.2022.971506.s002Test
https://figshare.com/articles/dataset/Table_1_Metabolomic_and_transcriptomic_analyses_reveal_the_mechanism_of_sweet-acidic_taste_formation_during_pineapple_fruit_development_XLSX/21061438Test

المؤلفون: Yuyao Gao, Yanli Yao, Xin Chen, Jianyang Wu, Qingsong Wu, Shenghui Liu, Anping Guo, Xiumei Zhang

مصطلحات موضوعية: Botany, Plant Biology, Plant Systematics and Taxonomy, Plant Cell and Molecular Biology, Plant Developmental and Reproductive Biology, Plant Pathology, Plant Physiology, Plant Biology not elsewhere classified, Ananas comosus, fruit quality, sucrose, citric acid, metabolic genes, transporter genes

الوصف: Pineapple (Ananas comosus L.) is one of the most valuable subtropical fruit crop in the world. The sweet-acidic taste of the pineapple fruits is a major contributor to the characteristic of fruit quality, but its formation mechanism remains elusive. Here, targeted metabolomic and transcriptomic analyses were performed during the fruit developmental stages in two pineapple cultivars (“Comte de Paris” and “MD-2”) to gain a global view of the metabolism and transport pathways involved in sugar and organic acid accumulation. Assessment of the levels of different sugar and acid components during fruit development revealed that the predominant sugar and organic acid in mature fruits of both cultivars was sucrose and citric acid, respectively. Weighted gene coexpression network analysis of metabolic phenotypes and gene expression profiling enabled the identification of 21 genes associated with sucrose accumulation and 19 genes associated with citric acid accumulation. The coordinated interaction of the 21 genes correlated with sucrose irreversible hydrolysis, resynthesis, and transport could be responsible for sucrose accumulation in pineapple fruit. In addition, citric acid accumulation might be controlled by the coordinated interaction of the pyruvate-to-acetyl-CoA-to-citrate pathway, gamma-aminobutyric acid pathway, and tonoplast proton pumps in pineapple. These results provide deep insights into the metabolic regulation of sweetness and acidity in pineapple.

العلاقة: https://figshare.com/articles/figure/Image_1_Metabolomic_and_transcriptomic_analyses_reveal_the_mechanism_of_sweet-acidic_taste_formation_during_pineapple_fruit_development_TIF/21061435Test

الإتاحة: https://doi.org/10.3389/fpls.2022.971506.s001Test
https://figshare.com/articles/figure/Image_1_Metabolomic_and_transcriptomic_analyses_reveal_the_mechanism_of_sweet-acidic_taste_formation_during_pineapple_fruit_development_TIF/21061435Test

المؤلفون: Dequan Sun, Guangming Sun, Xinhua Lu, Sheng-Hui Liu, Qingsong Wu

المصدر: Molecules
Molecules; Volume 19; Issue 6; Pages: 8518-8532
Molecules, Vol 19, Iss 6, Pp 8518-8532 (2014)

مصطلحات موضوعية: China, Sucrose, Antioxidant, Genotype, DPPH, medicine.medical_treatment, pineapple, genotypes, physico-chemical properties, antioxidant activity, minerals, Carbohydrates, Pharmaceutical Science, Ascorbic Acid, Ananas, Article, Antioxidants, Citric Acid, Analytical Chemistry, lcsh:QD241-441, chemistry.chemical_compound, lcsh:Organic chemistry, Phenols, Drug Discovery, Botany, medicine, Magnesium, Gallic acid, Food science, Physical and Theoretical Chemistry, Sugar, Flavonoids, Minerals, biology, Organic Chemistry, biology.organism_classification, Ascorbic acid, chemistry, Chemistry (miscellaneous), Potassium, Molecular Medicine, Calcium, Citric acid

الوصف: The fruit physico-chemical properties, antioxidant activity and mineral contents of 26 pineapple [Ananas comosus (L.) Merr.] genotypes grown in China were measured. The results showed great quantitative differences in the composition of these pineapple genotypes. Sucrose was the dominant sugar in all 26 genotypes, while citric acid was the principal organic acid. Potassium, calcium and magnesium were the major mineral constituents. The ascorbic acid (AsA) content ranged from 5.08 to 33.57 mg/100 g fresh weight (FW), while the total phenolic (TP) content varied from 31.48 to 77.55 mg gallic acid equivalents (GAE)/100 g FW. The two parameters in the predominant cultivars Comte de Paris and Smooth Cayenne were relative low. However, MD-2 indicated the highest AsA and TP contents (33.57 mg/100 g and 77.55 mg GAE/100 g FM, respectively), and it also showed the strongest antioxidant capacity 22.85 and 17.30 μmol TE/g FW using DPPH and TEAC methods, respectively. The antioxidant capacity of pineapple was correlated with the contents of phenolics, flavonoids and AsA. The present study provided important information for the further application of those pineapple genotypes.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d621d254ca55880819cc1b6e59e50e7dTest
http://europepmc.org/articles/PMC6270947Test