دورية أكاديمية
Sublingual flagellin protects against acute pneumococcal pneumonia in a TLR5-dependent and NLRC4-independent fashion.
| العنوان: | Sublingual flagellin protects against acute pneumococcal pneumonia in a TLR5-dependent and NLRC4-independent fashion. |
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| المؤلفون: | Muñoz-Wolf, Natalia, Rial, Analía, Fougeron, Delphine, Tabareau, Julien, Sirard, Jean-Claude, Chabalgoity, José, A |
| المساهمون: | School of Biochemistry and Immunology Dublin, Ireland, Trinity College Dublin, Facultad de Medicina Universidad de la Republica, Uruguay, Universidad de la República Montevideo (UDELAR), Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), This work was supported by Agencia Nacional de Investigación e Innovación (ANII)-Uruguay BE_POS_2010_1_2544, PR_FCE_2009_1_2783, Comisión Académica de Posgrado (CAP) Universidad de la República-Uruguay, Programa Nacional para Desarrollo de Ciencias Básicas (PEDECIBA)-Uruguay. INSERM, CNRS, Institut Pasteur de Lille and Université de Lille., The authors would like to thank BS M Muñoz-Wolf (Teaching Assistant of the Department of Quantitative Methods-School of Medicine-UdelaR) for his advice on statistical analysis and Prof EC Lavelle and Dr CP McEntee (Adjuvant Research Group, School of Biochemistry and Immunology, Trinity College Dublin) for the critical reading of the manuscript. |
| المصدر: | ISSN: 1746-0913. |
| بيانات النشر: | HAL CCSD Future Medicine |
| سنة النشر: | 2016 |
| المجموعة: | LillOA (HAL Lille Open Archive, Université de Lille) |
| مصطلحات موضوعية: | neutrophils, pneumonia, antimicrobial, flagellin, immunotherapy, Streptococcus pneumoniae, sublingual, TLR5, MESH: Administration, MESH: Animals, MESH: Humans, MESH: Lung/immunology, MESH: Bacterial Proteins/administration & dosage, MESH: Lung/microbiology, MESH: Mice, Inbred BALB C, MESH: Neutrophils/immunology, MESH: Pneumonia, Pneumococcal/genetics, Pneumococcal/immunology, Pneumococcal/microbiology, MESH: CARD Signaling Adaptor Proteins/genetics, Pneumococcal/prevention & control, MESH: Protein Domains, MESH: Streptococcus pneumoniae/genetics, MESH: Bacterial Proteins/chemistry, MESH: Streptococcus pneumoniae/immunology, MESH: Toll-Like Receptor 5/genetics, MESH: Toll-Like Receptor 5/immunology, MESH: Bacterial Proteins/genetics |
| الوصف: | International audience ; AIM: To evaluate efficacy of sublingual flagellin to treat acute pneumonia.MATERIALS & METHODS: Mice were treated sublingually with flagellin and challenged intranasally with a lethal dose of pneumococcus. Flagellins lacking TLR5 or NLRC4 activation domains were used to assess their contribution to protection.RESULTS: Sublingual flagellin protected mice in a TLR5-dependent, NLRC4-independent fashion. Neutrophils were required for protection. Flagellin-stimulated lung epithelial cells recapitulated the lung's transcriptional profile suggesting they could be targeted by flagellin in vivo.CONCLUSION: Ligation of TLR5, a pathogen recognition receptor not naturally engaged by pneumococcus, protects mice from invasive pneumonia when administered via sublingual route. This can be a highly cost-effective alternative therapy against pneumonia. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/27546231; inserm-01529275; https://inserm.hal.science/inserm-01529275Test; PUBMED: 27546231 |
| DOI: | 10.2217/fmb-2016-0045 |
| الإتاحة: | https://doi.org/10.2217/fmb-2016-0045Test https://inserm.hal.science/inserm-01529275Test |
| رقم الانضمام: | edsbas.9C172641 |
| قاعدة البيانات: | BASE |
| DOI: | 10.2217/fmb-2016-0045 |
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