PRP-19, a conserved pre-mRNA processing factor and E3 ubiquitin ligase, inhibits the nuclear accumulation of GLP-1/Notch intracellular domain
| العنوان: | PRP-19, a conserved pre-mRNA processing factor and E3 ubiquitin ligase, inhibits the nuclear accumulation of GLP-1/Notch intracellular domain |
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| المؤلفون: | Jorge Merlet, Yanwu Guo, Silvia Gutnik, Yann Thomas, Rafal Ciosk, Janosch Stoecklin, Lionel Pintard, Anca Neagu |
| المصدر: | Biology Open Biology Open, Vol 7, Iss 7 (2018) |
| بيانات النشر: | The Company of Biologists Ltd, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Notch, QH301-705.5, Science, Notch signaling pathway, Splicing, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Splicing factor, Ubiquitin, PRP-19, medicine, Biology (General), Caenorhabditis elegans, biology, biology.organism_classification, 3. Good health, Ubiquitin ligase, Cell biology, 030104 developmental biology, medicine.anatomical_structure, RNA splicing, biology.protein, Stem cell, General Agricultural and Biological Sciences, GLP-1, Germ cell, Research Article |
| الوصف: | The Notch signalling pathway is a conserved and widespread signalling paradigm, and its misregulation has been implicated in numerous disorders, including cancer. The output of Notch signalling depends on the nuclear accumulation of the Notch receptor intracellular domain (ICD). Using the Caenorhabditis elegans germline, where GLP-1/Notch-mediated signalling is essential for maintaining stem cells, we monitored GLP-1 in vivo. We found that the nuclear enrichment of GLP-1 ICD is dynamic: while the ICD is enriched in germ cell nuclei during larval development, it is depleted from the nuclei in adult germlines. We found that this pattern depends on the ubiquitin proteolytic system and the splicing machinery and, identified the splicing factor PRP-19 as a candidate E3 ubiquitin ligase required for the nuclear depletion of GLP-1 ICD. Summary: GFP tagging of endogenous GLP-1/Notch revealed life stage-specific differences in the nuclear accumulation of GLP-1 intracellular domain, which are mediated by the ubiquitin-proteolytic system and the splicing factor/E3 ubiquitin ligase PRP-19. |
| اللغة: | English |
| تدمد: | 2046-6390 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b7862c4dd498b1b239708427150e2b53Test http://europepmc.org/articles/PMC6078339Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b7862c4dd498b1b239708427150e2b53 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20466390 |
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