المؤلفون: Blondet, Antonine, Martin, Guillaume, Durand, Philippe, Perrard, Marie-Hélène

المساهمون: Kallistem SAS, Partenaires INRAE, VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Institut cellule souche et cerveau / Stem Cell and Brain Research Institute (SBRI), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

المصدر: ISSN: 0887-2333.

مصطلحات موضوعية: Spermatogenesis, In vitro model, Replacement of animals, Veterinary waste, Prepubertal domestic cat, Reproductive toxicology, [SDV.TOX]Life Sciences [q-bio]/Toxicology

الوصف: International audience ; In response to the EU cosmetics directive regulation and REACH legislation which encourage cell culture methods in order to reduce or replace the use of animals in toxicology studies, we settled the culture of prepubertal domestic cat seminiferous tubules in our validated BioAlter® model, usually used with prepubertal rat, called here BioAlter®-rat, by opposition to BioAlter®-cat settled here. We carried out a comparative study on the effects of 3 testicular toxicants, 1,3-dinitrobenzene at 60 μM, 2-methoxyacetic acid at 2.5 mM and carbendazim at 50 nM or 500 nM in both BioAlter®-cat and BioAlter®-rat over a 3-week culture period. Sertoli cell or each germ cell populations as well as the levels of Sertoli cell or germ cell specific mRNAs were studied. The harmful effects of the 3 toxicants on pre-meiotic, meiotic and post-meiotic cell numbers and on Sertoli or germ cell specific mRNAs were clearly observed in the two species, even if there might be some small differences in the intensity of the effects on some of the studied parameters. Hence, BioAlter®-cat might be a solution to the requirements of the EU cosmetics directive and REACH legislation for male reproductive toxicology studies.

العلاقة: hal-03761565; https://hal.science/hal-03761565Test; https://hal.science/hal-03761565/documentTest; https://hal.science/hal-03761565/file/Chat%20TIV%202022.pdfTest

الإتاحة: https://doi.org/10.1016/j.tiv.2022.105397Test
https://hal.science/hal-03761565Test
https://hal.science/hal-03761565/documentTest
https://hal.science/hal-03761565/file/Chat%20TIV%202022.pdfTest

المؤلفون: Blondet, Antonine, Martin, Guillaume, Paulic, Laurent, Perrard, Marie-Hélène, Durand, Philippe

المساهمون: Kallistem SAS, Partenaires INRAE, Institut cellule souche et cerveau (SBRI), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)

المصدر: ISSN: 0944-1344.

مصطلحات موضوعية: Water quality, in vitro bioassay, methods, spermatogenesis, blood-testis barrier integrity, endocrine disrupting effects, global toxicity, [SDV.TOX]Life Sciences [q-bio]/Toxicology, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology

الوصف: International audience ; Many toxicants are present in water as a mixture. Male infertility is one of the environmental impacts in developed countries. Using our rat seminiferous tubule culture model, we evaluated the effects of waters of different origins, on several parameters of the seminiferous epithelium. Concentrated culture medium was diluted with the waters to be tested (final concentrations of the tested waters were between 8 and 80%). The integrity of the blood-testis barrier was assessed by the trans-epithelial electric resistance (TEER). The levels of mRNAs specific of Sertoli cells, of cellular junctions, of each population of germ cells, of androgen receptor, of estrogen receptor α and of aromatase were also studied. We report, here, the results obtained with ten waters, some of them possessing a negative effect on spermatogenesis. The results showed that according to the tested waters, their effects on the parameters studied might be quite different indicating many different mechanisms of toxicity, including some endocrine disrupting effects. It has been reported that men with impaired semen parameters have an increased mortality rate suggesting semen quality may provide a fundamental biomarker of overall male health. Hence, we have developed a relevant in vitro bioassay allowing to evaluate the potential toxicity of different types of waters on male fertility and to assess some aspects of their mechanism of action. In addition to the TEER measure, the number and/or the identity of the studied mRNAs can be largely increased and/or modified, thus enhancing the possibility of using this model as a "warning system".

العلاقة: hal-03234425; https://hal.science/hal-03234425Test; https://hal.science/hal-03234425/documentTest; https://hal.science/hal-03234425/file/An%20in-vitro%20bioassay%20to%20assess%20the%20potential%20global%20toxicity%20of%20waters%20on%20spermatogenesis%20a%20pilot%20study%20x.pdfTest

الإتاحة: https://doi.org/10.1007/s11356-021-12480-1Test
https://hal.science/hal-03234425Test
https://hal.science/hal-03234425/documentTest
https://hal.science/hal-03234425/file/An%20in-vitro%20bioassay%20to%20assess%20the%20potential%20global%20toxicity%20of%20waters%20on%20spermatogenesis%20a%20pilot%20study%20x.pdfTest

المؤلفون: Blondet Antonine, Martin Guillaume, Durand Philippe, Perrard Marie-Hélène

المساهمون: Kallistem SAS, Partenaires INRAE, VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Institut cellule souche et cerveau / Stem Cell and Brain Research Institute (U1208 Inserm - UCBL1 / SBRI - USC 1361 INRAE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Perrard, Marie-Hélène, Kallistem SAS Vetagro Sup Marcy l'Etoile

المصدر: Toxicology in Vitro
Toxicology in Vitro, 2022, 79, pp.105291. ⟨10.1016/j.tiv.2021.105291⟩
Toxicology in Vitro, Elsevier, 2022, 79, pp.105291. ⟨10.1016/j.tiv.2021.105291⟩

مصطلحات موضوعية: Male, Glyphosate, [SDV]Life Sciences [q-bio], Glycine, In vitro study, General Medicine, Low concentrations, Seminiferous Tubules, Toxicology, Spermatogonia, [SDV] Life Sciences [q-bio], Rats, Sprague-Dawley, Tissue Culture Techniques, Meiosis, Clusterin, Spermatocytes, [SDV.TOX]Life Sciences [q-bio]/Toxicology, Animals, RNA, Messenger, Spermatogenesis

الوصف: International audience; Glyphosate is the most used herbicide in the world. Controversial studies exist on its effect on the male reproductive system. We used the validated BioAlter® model to test the effects of low concentrations of Glyphosate. Pubertal rat seminiferous tubules were treated with Glyphosate 50 nM, 500 nM, 5 μM or 50 μM over a 3-week culture period. The Trans-Epithelial Electrical Resistance was not modified by any of the concentrations. The decrease of Clusterin mRNAs suggested that glyphosate would target the integrity of Sertoli cells. The decrease of the numbers of germ cells from day 14 onward highlighted the chronic effect of glyphosate at 50 nM, 500 nM or 5 μM. No consistent effect of glyphosate was observed on the numbers of spermatogonia or on their specific mRNA levels. However, those low concentrations of glyphosate targeted young spermatocytes and middle to late pachytene spermatocytes resulting in a decrease of the numbers of round spermatids, the direct precursors of spermatozoa. This study underlines that the effect of a toxicant should be also studied at low doses and during the establishment of the blood-testis barrier.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::877d847c60bb7b8ed569dccc58c99831Test
https://pubmed.ncbi.nlm.nih.gov/34864054Test

المؤلفون: Pisani, Cedric, Voisin, Sebastien, Arafah, Karim, Durand, Philippe, Perrard, Marie-Hélène, Guichaoua, Marie-Roberte, Bulet, Philippe, Prat, Odette

المساهمون: Communications Cellulaires et Différenciation (CCD), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement IRD : UMR237-Centre National de la Recherche Scientifique (CNRS), Atheris Laboratories, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), ANSES, the French Agency for Food, Environmental and Occupational Health Safety EST-2010-2-019

المصدر: ISSN: 1868-596X.

مصطلحات موضوعية: spermatogenesis, endocrine disruption, biomarker, pesticides, toxicogenomics, [SDE.BE]Environmental Sciences/Biodiversity and Ecology

الوصف: International audience ; To measure the testicular toxicity of two fungicides (carbendazim and iprodione), alone or in a mixture, we used a rat ex vivo model of seminiferous tubules, greatly reducing the number of rodents used, in accordance with the 3R rule (Replacement, Reduction, and Refinement). This model allows the representation of puberty, a critical life period with regard to endocrine disruptors. The cellular modifications were followed for three weeks through transcriptomic and proteomic profiling analysis. A quantitative and comparative method was developed to estimate how known pathways were disturbed by each substance. This pathway-driven analysis revealed a strong alteration of steroidogenesis and an impairment of meiosis in all cases, albeit the initial molecular events were different for both substances. The ex vivo cytogenetic analysis confirmed that both fungicides alter the course of the first meiotic prophase. In addition, the mixture of both substances triggered effects greater than the sum of their cumulative effects and compromised future sperm motility after a shorter time of exposure compared with the fungicides tested separately. The alliance of an ex vivo culture with omics strategies complemented with a physiological examination is a powerful combination of tools for testing substances, separately or in a mixture, for their testicular toxicity. In particular, proteomics allowed the identification of systematically differentially expressed proteins in the secretomes of exposed cultures, such as FUCO and PEBP1, two proteins linked with the motility and fertilizing ability of spermatozoa, respectively. These proteins may be potential biomarkers of testicular dysfunction and infertility.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/27155993; hal-01444080; https://hal.science/hal-01444080Test; https://hal.science/hal-01444080/documentTest; https://hal.science/hal-01444080/file/2016_Pisani_Altex_1.pdfTest; PRODINRA: 389549; PUBMED: 27155993; WOS: 000387439200004

الإتاحة: https://doi.org/10.14573/altex.1601253Test
https://hal.science/hal-01444080Test
https://hal.science/hal-01444080/documentTest
https://hal.science/hal-01444080/file/2016_Pisani_Altex_1.pdfTest

المؤلفون: Durand, Philippe, ́, Blondet, Antonine, Martin, Guillaume, Carette, Diane, Pointis, Georges, Perrard, Marie-Hélène

المساهمون: Institut de Chimie des Substances Naturelles (ICSN), Institut de Chimie - CNRS Chimie (INC-CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Communications Cellulaires et Différenciation (CCD), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Kallistem SAS, Partenaires INRAE, Essais Thérapeutiques et Maladies Infectieuses, Université Paris-Sud - Paris 11 (UP11)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre méditérannéen de médecine moléculaire (C3M), Université Nice Sophia Antipolis (1965 - 2019) (UNS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Inflammasome NLRP3 – NLRP3 Inflammasome, Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 0887-2333.

مصطلحات موضوعية: Atrazine, Benzo[a]pyrene, Low doses, Micropollutants, Seminiferous tubule culture model, Spermatogenesis, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, [SDV.EE]Life Sciences [q-bio]/Ecology, environment

الوصف: International audience ; Atrazine (ATZ), a widely used agricultural pesticide and benzo[a]pyrene (BaP), a ubiquitous environmental human carcinogen can induce alterations of spermatogenesis. In the present study, we showed first that our seminiferous tubule culture model, in bicameral chambers, allowed the settlement of the blood-testis barrier (BTB) in 8-day-old male rat cultures and the differentiation of spermatogonia into round spermatids.The effect of a mixture of 1 μg/L of ATZ and 1 μg/L of BaP was then investigated either during or after the establishment of the BTB by using 8- or 20-22-day-old rats. Cultures were performed over a 3-week period. Our results show that claudin-11 and connexin 43 two proteins of the BTB, were impaired by the mixture which also reduced the number of round spermatids (the direct precursors of spermatozoa), by targeting the middle to late pachytene spermatocytes. These effects were observed in 8- and 20-22-day -old rat seminiferous tubule cultures. However, the decrease of the number of round spermatids was faster and more marked in the 8-day- than in the 20-22-day -old rat seminiferous tubule cultures. Our study emphasizes the possible influence of the age of an individual on the effect of (a) toxicant(s) on spermatogenesis.

العلاقة: hal-02870404; https://hal.science/hal-02870404Test

الإتاحة: https://doi.org/10.1016/j.tiv.2019.104699Test
https://hal.science/hal-02870404Test

المؤلفون: Durand, Philippe, ́, Blondet, Antonine, Gilleron, Jérôme, Carette, Diane, Janczarski, Stéphane, Christin, Emilie, Pointis, Georges, Perrard, Marie-Hélène, Martin, Guillaume

المساهمون: Institut de Chimie des Substances Naturelles (ICSN), Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), Communications Cellulaires et Différenciation (CCD), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre méditerranéen de médecine moléculaire (C3M), Université Nice Sophia Antipolis (1965 - 2019) (UNS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UniCA), Essais Thérapeutiques et Maladies Infectieuses, Université Paris-Sud - Paris 11 (UP11)-Institut National de la Santé et de la Recherche Médicale (INSERM), Reproduction et développement des plantes (RDP), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), École normale supérieure de Lyon (ENS de Lyon), Institut de Génomique Fonctionnelle de Lyon (IGFL), ANSES (TOTEM) EST-2010/2/019, KALLISTEM

المصدر: ISSN: 0887-2333.

مصطلحات موضوعية: Mixture, Carbendazim, Iprodione, Low doses, Spermatogenesis, ex vivo study, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, [SDV.EE]Life Sciences [q-bio]/Ecology, environment

الوصف: International audience ; It has been shown that non-cytotoxic doses of Carbendazim (CBZ), a broad-spectrum benzimidazole fungicide, possess endocrine-disrupting (androgen-like) actions, ex vivo, on the pubertal rat seminiferous epithelium. Iprodione (IPR), a dicarboximide fungicide, is also known to be an endocrine-disrupter (anti-androgen). The effect of a mixture of these two pesticides was investigated in the validated rat seminiferous tubule culture model. Cultures were performed in the absence or presence of CBZ 50 nM or IPR 50 nM either alone or in mixture (Mix), over a 3-week period. Mix exerted a dramatic effect on two proteins (Connexin 43 and Claudin-11) of the blood-testis barrier and possessed similar effects to IPR on some germ cell populations. The presence of IPR together with CBZ (Mix) cancelled the effect of CBZ on the increase of the androgen-dependent TP1 and TP2 mRNAs and on the decrease of ER alpha, ER beta mRNAs. Nevertheless, CBZ alone or IPR alone or Mix induced toxicity on spermatogenesis resulting in a decrease of round spermatids (the precursors of spermatozoa). These results strongly suggest that, even at these low concentrations, the effects of IPR and of CBZ are not solely dependent on their respective anti-androgenic and androgen-like effects and should involve several mechanisms of action.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/28576679; hal-02870440; https://hal.science/hal-02870440Test; PUBMED: 28576679; WOS: 000419424500012

الإتاحة: https://doi.org/10.1016/j.tiv.2017.05.022Test
https://hal.science/hal-02870440Test

المؤلفون: Perrard, Marie-Hélène, Durand, Philippe

المساهمون: Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Marc R. Pelletier (Editeur)

المصدر: Strategic Aspects of Spermatogenesis and the Maturation of Spermatozoa. Part I ; https://hal.inrae.fr/hal-02814190Test ; Strategic Aspects of Spermatogenesis and the Maturation of Spermatozoa. Part I, 72 (8), Wiley, 2009, Microscopy Research and Technique

مصطلحات موضوعية: SPERMATOGENESIS, GROWTH FACTOR, RAT, MEIOSIS, NEUROTROPHIN, INTRATESTICULAR REGULATION, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience

العلاقة: hal-02814190; https://hal.inrae.fr/hal-02814190Test; PRODINRA: 31292

الإتاحة: https://hal.inrae.fr/hal-02814190Test

المؤلفون: Carette, Diane, Perrard, Marie-Hélène, Prisant, Nadia, Gilleron, Jérôme, Pointis, Georges, Segretain, Dominique, Durand, Philippe

المساهمون: Université de Nice Sophia-Antipolis (UNSA), UMR S775, Université Paris Descartes - Paris 5 (UPD5), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Kallistem SAS, Partenaires INRAE

المصدر: ISSN: 0041-008X.

مصطلحات موضوعية: Hexavalent chromium, Blood-testis barrier, Spermatogenesis, The rat seminiferous tubule culture model, IN-VITRO, CADMIUM CHLORIDE, PROLIFERATION, EPITHELIUM, Junctional proteins, INTERCELLULAR COMMUNICATION, SPERMATOGENIC CELLS, EXPRESSION, EXPOSURE, SYSTEM, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; Exposure to toxic metals, specifically those belonging to the nonessential group leads to human health defects and among them reprotoxic effects. The mechanisms by which these metals produce their negative effects on spermatogenesis have not been fully elucidated. By using the Durand's validated seminiferous tubule culture model, which mimics the in vivo situation, we recently reported that concentrations of hexavalent chromium, reported in the literature to be closed to that found in the blood circulation of men, increase the number of germ cell cytogenetic abnormalities. Since this metal is also known to affect cellular junctions, we investigated, in the present study, its potential influence on the Sertoli cell barrier and on junctional proteins present at this level such as connexin 43, claudin-11 and N-cadherin. Cultured seminiferous tubules in bicameral chambers expressed the three junctional proteins and ZO-1 for at least 12 days. Exposure to low concentrations of chromium (10 mu g/l) increased the trans-epithelial resistance without major changes of claudin-11 and N-cadherin expressions but strongly delocalized the gap junction protein connexin 43 from the membrane to the cytoplasm of Sertoli cells. The possibility that the hexavalent chromium-induced alteration of connexin 43 indirectly mediates the effect of the toxic metal on the blood testis barrier dynamic is postulated. (c) 2013 Elsevier Inc. All rights reserved.

العلاقة: hal-02651875; https://hal.inrae.fr/hal-02651875Test; PRODINRA: 209151; WOS: 000316305600003

الإتاحة: https://doi.org/10.1016/j.taap.2013.01.016Test
https://hal.inrae.fr/hal-02651875Test

المؤلفون: Geoffroy-Siraudin, Cendrine, Perrard, Marie-Hélène, Ghalamoun-Slaimi, Rahma, Ali, Sazan, Chaspoul, Florence, Lanteaume, Andre, Achard, Vincent, Gallice, Philippe, Durand, Philippe, Guichaoua, Marie-Roberte

المساهمون: Université de la Méditerranée - Aix-Marseille 2, Assistance Publique - Hôpitaux de Marseille (APHM), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 0041-008X.

مصطلحات موضوعية: Cadmium, Rat, Spermatogenesis, IN-VITRO MODEL, BLOOD, SPERMATOCYTES, DISRUPTION, ACTIVATION, Synaptonemal complex, SYNAPTONEMAL COMPLEX DAMAGE, RAT TESTIS, SPERMATOGENIC CELLS, MEIOSIS, POPULATIONS, Culture, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; Using a validated model of culture of rat seminiferous tubules, we assessed the effects of 0.1, 1 and 10 mu g/L cadmium (Cd) on spermatogenic cells over a 2-week culture period. With concentrations of 1 and 10 mu g/L in the culture medium, the Cd concentration in the cells, determined by ICP-MS, increased with concentration in the medium and the day of culture. Flow cytometric analysis enabled us to evaluate changes in the number of Sertoli cells and germ cells during the culture period. The number of Sertoli cells did not appear to be affected by Cd. By contrast, spermatogonia and meiotic cells were decreased by 1 and 10 mu g/L Cd in a time and dose dependent manner. Stage distribution of the meiotic prophase I and qualitative study of the synaptonemal complexes (SC) at the pachytene stage were performed by immunocytochemistry with an anti SCP3 antibody. Cd caused a time-and-dose-dependent increase of total abnormalities, of fragmented SC and of asynapsis from concentration of 0.1 mu g/L. Additionally, we observed a new SC abnormality, the "motheaten" SC. This abnormality is frequently associated with asynapsis and SC widening which increased with both the Cd concentration and the duration of exposure. This abnormality suggests that Cd disrupts the structure and function of proteins involved in pairing and/or meiotic recombination. These results show that Cd induces dose-and-time-dependent alterations of the meiotic process of spermatogenesis ex-vivo, and that the lowest metal concentration, which induces an adverse effect, may vary with the cell parameter studied. (C) 2012 Elsevier Inc. All rights reserved.

العلاقة: hal-02644732; https://hal.inrae.fr/hal-02644732Test; PRODINRA: 214384; WOS: 000306888800004

الإتاحة: https://doi.org/10.1016/j.taap.2012.04.033Test
https://hal.inrae.fr/hal-02644732Test

العنوان البديل: Ex vivo spermatogenesis.

المؤلفون: Perrard, Marie-Hélène¹ marie-helene.perrarddurand@inserm.fr, Durand, Philippe²

المصدر: MT: Médecine de la Reproduction, Gynécologie et Endocrinologie. oct-dec2017, Vol. 19 Issue 4, p279-289. 11p.

مصطلحات موضوعية: *SPERMATOGENESIS, *SERTOLI cells, *GERM cells, *SEMINIFEROUS tubules, *HUMAN fertility

الملخص (بالإنجليزية): For a century, researchers have been working on the realization of ex vivo spermatogenesis. Four culture systems were tested: organotypic culture, coculture of Sertoli cells and germ cells, culture of seminiferous tubules and three-dimensional culture in biomaterials. Conservation of the structure of the seminiferous tubules in the following systems has made this success possible : Ogawa's organotypic culture system and the three-dimensional culture system of Huleihel and Schlatt made it possible to carry out complete spermatogenesis ex vivo in mice. The Artistem® culture system of our team made possible ex vivo complete spermatogenesis of the rat, the monkey and the man by confining the seminiferous tubules in a chitosan bioreactor. The realization of spermatogenesis ex vivo allows identifying the factors involved in the local regulation of spermatogenesis, and the molecules harmful for spermatogenesis which are present in our environment. Some of these systems could be also used to preserve the fertility of young boys with cancer and to restore the fertility of patients suffering from secretory azoospermia due to a somatic deficiency. Before clinical studies, it will be necessary to perform the proof of concept in a non-rodent species, the monkey, and to show the genetic and epigenetic normality of the ex vivo differentiated human spermatozoa. [ABSTRACT FROM AUTHOR]

Abstract (French): Pendant un siècle, des chercheurs ont travaillé à la réalisation de la spermatogenèse ex vivo. Quatre systèmes de culture ont ainsi été proposés et testés : la culture organotypique, la coculture de cellules de Sertoli et de cellules germinales, la culture de tubes séminifères et la culture tridimensionnelle dans des biomatériaux. La conservation de la structure du tube séminifère dans plusieurs systèmes a permis cette réussite : le système de culture organotypique d'Ogawa et le système de culture tridimensionnel de Huleihel et Schlatt ont permis de réaliser toute la spermatogenèse ex vivo chez la souris. Le système de culture, Artistem®, élaboré par notre équipe, a permis d'effectuer ex vivo toute la spermatogenèse du rat, du singe et de l'homme en confinant les tubes séminifères dans un bioréacteur de chitosan. La réalisation de la spermatogenèse ex vivo permet d'identifier les facteurs impliqués dans les régulations locales de la spermatogenèse, et les molécules néfastes pour la spermatogenèse présentes dans notre environnement. Certains de ces systèmes pourraient être utilisés pour préserver la fertilité de jeunes garc¸ons atteints de cancer ou restaurer celle de patients souffrant d'une azoospermie sécrétoire, due à une déficience somatique. Avant les études cliniques, il faudra faire la preuve du concept dans une espèce non rongeur, le singe, et montrer les normalités génétique et épigénétique des spermatozoïdes humains obtenus. [ABSTRACT FROM AUTHOR]