المؤلفون: Geoffroy-Siraudin, Cendrine, Perrard, Marie-Hélène, Ghalamoun-Slaimi, Rahma, Ali, Sazan, Chaspoul, Florence, Lanteaume, Andre, Achard, Vincent, Gallice, Philippe, Durand, Philippe, Guichaoua, Marie-Roberte

المساهمون: Université de la Méditerranée - Aix-Marseille 2, Assistance Publique - Hôpitaux de Marseille (APHM), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 0041-008X.

مصطلحات موضوعية: Cadmium, Rat, Spermatogenesis, IN-VITRO MODEL, BLOOD, SPERMATOCYTES, DISRUPTION, ACTIVATION, Synaptonemal complex, SYNAPTONEMAL COMPLEX DAMAGE, RAT TESTIS, SPERMATOGENIC CELLS, MEIOSIS, POPULATIONS, Culture, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; Using a validated model of culture of rat seminiferous tubules, we assessed the effects of 0.1, 1 and 10 mu g/L cadmium (Cd) on spermatogenic cells over a 2-week culture period. With concentrations of 1 and 10 mu g/L in the culture medium, the Cd concentration in the cells, determined by ICP-MS, increased with concentration in the medium and the day of culture. Flow cytometric analysis enabled us to evaluate changes in the number of Sertoli cells and germ cells during the culture period. The number of Sertoli cells did not appear to be affected by Cd. By contrast, spermatogonia and meiotic cells were decreased by 1 and 10 mu g/L Cd in a time and dose dependent manner. Stage distribution of the meiotic prophase I and qualitative study of the synaptonemal complexes (SC) at the pachytene stage were performed by immunocytochemistry with an anti SCP3 antibody. Cd caused a time-and-dose-dependent increase of total abnormalities, of fragmented SC and of asynapsis from concentration of 0.1 mu g/L. Additionally, we observed a new SC abnormality, the "motheaten" SC. This abnormality is frequently associated with asynapsis and SC widening which increased with both the Cd concentration and the duration of exposure. This abnormality suggests that Cd disrupts the structure and function of proteins involved in pairing and/or meiotic recombination. These results show that Cd induces dose-and-time-dependent alterations of the meiotic process of spermatogenesis ex-vivo, and that the lowest metal concentration, which induces an adverse effect, may vary with the cell parameter studied. (C) 2012 Elsevier Inc. All rights reserved.

العلاقة: hal-02644732; https://hal.inrae.fr/hal-02644732Test; PRODINRA: 214384; WOS: 000306888800004

الإتاحة: https://doi.org/10.1016/j.taap.2012.04.033Test
https://hal.inrae.fr/hal-02644732Test

المؤلفون: Geoffroy-Siraudin, Cendrine, Perrard, Marie-Hélène, Ghalamoun-Slaimi, Rahma, Ali, Sazan, Chaspoul, Florence, Lanteaume, Andre, Achard, Vincent, Gallice, Philippe, Durand, Philippe, Guichaoua, Marie-Roberte

المساهمون: Université de la Méditerranée - Aix-Marseille 2, Assistance Publique - Hôpitaux de Marseille (APHM), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 0041-008X.

مصطلحات موضوعية: Cadmium, Rat, Spermatogenesis, IN-VITRO MODEL, BLOOD, SPERMATOCYTES, DISRUPTION, ACTIVATION, Synaptonemal complex, SYNAPTONEMAL COMPLEX DAMAGE, RAT TESTIS, SPERMATOGENIC CELLS, MEIOSIS, POPULATIONS, Culture, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; Using a validated model of culture of rat seminiferous tubules, we assessed the effects of 0.1, 1 and 10 mu g/L cadmium (Cd) on spermatogenic cells over a 2-week culture period. With concentrations of 1 and 10 mu g/L in the culture medium, the Cd concentration in the cells, determined by ICP-MS, increased with concentration in the medium and the day of culture. Flow cytometric analysis enabled us to evaluate changes in the number of Sertoli cells and germ cells during the culture period. The number of Sertoli cells did not appear to be affected by Cd. By contrast, spermatogonia and meiotic cells were decreased by 1 and 10 mu g/L Cd in a time and dose dependent manner. Stage distribution of the meiotic prophase I and qualitative study of the synaptonemal complexes (SC) at the pachytene stage were performed by immunocytochemistry with an anti SCP3 antibody. Cd caused a time-and-dose-dependent increase of total abnormalities, of fragmented SC and of asynapsis from concentration of 0.1 mu g/L. Additionally, we observed a new SC abnormality, the "motheaten" SC. This abnormality is frequently associated with asynapsis and SC widening which increased with both the Cd concentration and the duration of exposure. This abnormality suggests that Cd disrupts the structure and function of proteins involved in pairing and/or meiotic recombination. These results show that Cd induces dose-and-time-dependent alterations of the meiotic process of spermatogenesis ex-vivo, and that the lowest metal concentration, which induces an adverse effect, may vary with the cell parameter studied. (C) 2012 Elsevier Inc. All rights reserved.

العلاقة: hal-02644732; https://hal.inrae.fr/hal-02644732Test; PRODINRA: 214384; WOS: 000306888800004

الإتاحة: https://doi.org/10.1016/j.taap.2012.04.033Test
https://hal.inrae.fr/hal-02644732Test

المؤلفون: Geoffroy-Siraudin, Cendrine, Perrard, Marie-Hélène, Ghalamoun-Slaimi, Rahma, Ali, Sazan, Chaspoul, Florence, Lanteaume, Andre, Achard, Vincent, Gallice, Philippe, Durand, Philippe, Guichaoua, Marie-Roberte

المساهمون: Université de la Méditerranée - Aix-Marseille 2, Assistance Publique - Hôpitaux de Marseille (APHM), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 0041-008X.

مصطلحات موضوعية: Cadmium, Rat, Spermatogenesis, IN-VITRO MODEL, BLOOD, SPERMATOCYTES, DISRUPTION, ACTIVATION, Synaptonemal complex, SYNAPTONEMAL COMPLEX DAMAGE, RAT TESTIS, SPERMATOGENIC CELLS, MEIOSIS, POPULATIONS, Culture, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; Using a validated model of culture of rat seminiferous tubules, we assessed the effects of 0.1, 1 and 10 mu g/L cadmium (Cd) on spermatogenic cells over a 2-week culture period. With concentrations of 1 and 10 mu g/L in the culture medium, the Cd concentration in the cells, determined by ICP-MS, increased with concentration in the medium and the day of culture. Flow cytometric analysis enabled us to evaluate changes in the number of Sertoli cells and germ cells during the culture period. The number of Sertoli cells did not appear to be affected by Cd. By contrast, spermatogonia and meiotic cells were decreased by 1 and 10 mu g/L Cd in a time and dose dependent manner. Stage distribution of the meiotic prophase I and qualitative study of the synaptonemal complexes (SC) at the pachytene stage were performed by immunocytochemistry with an anti SCP3 antibody. Cd caused a time-and-dose-dependent increase of total abnormalities, of fragmented SC and of asynapsis from concentration of 0.1 mu g/L. Additionally, we observed a new SC abnormality, the "motheaten" SC. This abnormality is frequently associated with asynapsis and SC widening which increased with both the Cd concentration and the duration of exposure. This abnormality suggests that Cd disrupts the structure and function of proteins involved in pairing and/or meiotic recombination. These results show that Cd induces dose-and-time-dependent alterations of the meiotic process of spermatogenesis ex-vivo, and that the lowest metal concentration, which induces an adverse effect, may vary with the cell parameter studied. (C) 2012 Elsevier Inc. All rights reserved.

العلاقة: hal-02644732; https://hal.inrae.fr/hal-02644732Test; PRODINRA: 214384; WOS: 000306888800004

الإتاحة: https://doi.org/10.1016/j.taap.2012.04.033Test
https://hal.inrae.fr/hal-02644732Test

المؤلفون: Geoffroy-Siraudin, Cendrine, Perrard, Marie-Hélène, Ghalamoun-Slaimi, Rahma, Ali, Sazan, Chaspoul, Florence, Lanteaume, Andre, Achard, Vincent, Gallice, Philippe, Durand, Philippe, Guichaoua, Marie-Roberte

المساهمون: Université de la Méditerranée - Aix-Marseille 2, Assistance Publique - Hôpitaux de Marseille (APHM), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 0041-008X.

مصطلحات موضوعية: Cadmium, Rat, Spermatogenesis, IN-VITRO MODEL, BLOOD, SPERMATOCYTES, DISRUPTION, ACTIVATION, Synaptonemal complex, SYNAPTONEMAL COMPLEX DAMAGE, RAT TESTIS, SPERMATOGENIC CELLS, MEIOSIS, POPULATIONS, Culture, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; Using a validated model of culture of rat seminiferous tubules, we assessed the effects of 0.1, 1 and 10 mu g/L cadmium (Cd) on spermatogenic cells over a 2-week culture period. With concentrations of 1 and 10 mu g/L in the culture medium, the Cd concentration in the cells, determined by ICP-MS, increased with concentration in the medium and the day of culture. Flow cytometric analysis enabled us to evaluate changes in the number of Sertoli cells and germ cells during the culture period. The number of Sertoli cells did not appear to be affected by Cd. By contrast, spermatogonia and meiotic cells were decreased by 1 and 10 mu g/L Cd in a time and dose dependent manner. Stage distribution of the meiotic prophase I and qualitative study of the synaptonemal complexes (SC) at the pachytene stage were performed by immunocytochemistry with an anti SCP3 antibody. Cd caused a time-and-dose-dependent increase of total abnormalities, of fragmented SC and of asynapsis from concentration of 0.1 mu g/L. Additionally, we observed a new SC abnormality, the "motheaten" SC. This abnormality is frequently associated with asynapsis and SC widening which increased with both the Cd concentration and the duration of exposure. This abnormality suggests that Cd disrupts the structure and function of proteins involved in pairing and/or meiotic recombination. These results show that Cd induces dose-and-time-dependent alterations of the meiotic process of spermatogenesis ex-vivo, and that the lowest metal concentration, which induces an adverse effect, may vary with the cell parameter studied. (C) 2012 Elsevier Inc. All rights reserved.

العلاقة: hal-02644732; https://hal.inrae.fr/hal-02644732Test; PRODINRA: 214384; WOS: 000306888800004

الإتاحة: https://doi.org/10.1016/j.taap.2012.04.033Test
https://hal.inrae.fr/hal-02644732Test

المؤلفون: Geoffroy-Siraudin, Cendrine, Perrard, Marie-Hélène, Ghalamoun-Slaimi, Rahma, Ali, Sazan, Chaspoul, Florence, Lanteaume, Andre, Achard, Vincent, Gallice, Philippe, Durand, Philippe, Guichaoua, Marie-Roberte

المساهمون: Université de la Méditerranée - Aix-Marseille 2, Assistance Publique - Hôpitaux de Marseille (APHM), Institut de Génomique Fonctionnelle de Lyon (IGFL), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon)

المصدر: ISSN: 0041-008X.

مصطلحات موضوعية: Cadmium, Rat, Spermatogenesis, IN-VITRO MODEL, BLOOD, SPERMATOCYTES, DISRUPTION, ACTIVATION, Culture, Meiosis, Synaptonemal complex, SYNAPTONEMAL COMPLEX DAMAGE, RAT TESTIS, SPERMATOGENIC CELLS, POPULATIONS, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; Using a validated model of culture of rat seminiferous tubules, we assessed the effects of 0.1, 1 and 10 mu g/L cadmium (Cd) on spermatogenic cells over a 2-week culture period. With concentrations of 1 and 10 mu g/L in the culture medium, the Cd concentration in the cells, determined by ICP-MS, increased with concentration in the medium and the day of culture. Flow cytometric analysis enabled us to evaluate changes in the number of Sertoli cells and germ cells during the culture period. The number of Sertoli cells did not appear to be affected by Cd. By contrast, spermatogonia and meiotic cells were decreased by 1 and 10 mu g/L Cd in a time and dose dependent manner. Stage distribution of the meiotic prophase I and qualitative study of the synaptonemal complexes (SC) at the pachytene stage were performed by immunocytochemistry with an anti SCP3 antibody. Cd caused a time-and-dose-dependent increase of total abnormalities, of fragmented SC and of asynapsis from concentration of 0.1 mu g/L. Additionally, we observed a new SC abnormality, the "motheaten" SC. This abnormality is frequently associated with asynapsis and SC widening which increased with both the Cd concentration and the duration of exposure. This abnormality suggests that Cd disrupts the structure and function of proteins involved in pairing and/or meiotic recombination. These results show that Cd induces dose-and-time-dependent alterations of the meiotic process of spermatogenesis ex-vivo, and that the lowest metal concentration, which induces an adverse effect, may vary with the cell parameter studied. (C) 2012 Elsevier Inc. All rights reserved.

العلاقة: hal-02644732; https://hal.inrae.fr/hal-02644732Test; PRODINRA: 214384; WOS: 000306888800004

الإتاحة: https://doi.org/10.1016/j.taap.2012.04.033Test
https://hal.inrae.fr/hal-02644732Test

المؤلفون: Geoffroy-Siraudin, Cendrine, Perrard, Marie-Hélène, Chaspoul, Florence, Lanteaume, Andre, Gallice, Philippe, Durand, Philippe, Guichaoua, Marie-Roberte

المساهمون: Biogénotoxicologie et Mutagenèse Environnementale (BME), Université de la Méditerranée - Aix-Marseille 2, Centre National de la Recherche Scientifique (CNRS), Assistance Publique - Hôpitaux de Marseille (APHM), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), CNRS, INRA, Université Claude Bernard Lyon I, Ecole Normale supérieure de Lyon, Université de Méditerranée

المصدر: ISSN: 1096-6080.

مصطلحات موضوعية: spermatogenesis, rat, hexavalent chromium, in-vitro, chromosome, spermatocytes, quality, meiosis, culture, synaptonemal complex, synaptonemal complex damage, spermatogenic cells, sertoli-cells, germ-cells, population, exposure, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; There is evidence that exposure to environmental factors is at least partly responsible for changes in semen quality observed over the past decades. The detection of reproductive toxicants under Registration, Evaluation and Authorisation of Chemicals (REACH) will impact animal use for regulatory safety testing. We first validated a model of culture of rat seminiferous tubules for toxicological studies on spermatogenesis. Then, using this model of culture, we assessed the deleterious effects of 1, 10, and 100 μg/l hexavalent chromium [Cr(VI)] on meiotic cells. The prophase I of meiosis was studied in vivo and ex vivo. Bromo-2′-deoxyuridine (BrdU) was used to describe the kinetics of germ cell differentiation. SCP3 labeling allowed to establish the distribution of the stages of the meiotic prophase I and to perform a qualitative study of the pachytene stage in the absence or presence of Cr(VI). The development of the meiotic step of pubertal rats was similar in vivo and ex vivo. The number of total cells appeared not affected by the presence of Cr(VI) irrespective of its concentration. However, the numbers of late spermatocytes and of round spermatids were decreased by Cr(VI) even at the lower concentration. The percentage of synaptonemal complex abnormalities increased slightly with the time of culture and dramatically with Cr(VI) concentrations. This model of culture appears suitable for toxicological studies. This study shows that Cr(VI) is toxic for meiotic cells even at low concentrations, and its toxicity increases in a dose-dependent manner.

العلاقة: hal-02665346; https://hal.inrae.fr/hal-02665346Test; PRODINRA: 225899; WOS: 000279316500028

الإتاحة: https://doi.org/10.1093/toxsci/kfq099Test
https://hal.inrae.fr/hal-02665346Test

المؤلفون: Geoffroy-Siraudin, Cendrine, Perrard, Marie-Hélène, Chaspoul, Florence, Lanteaume, Andre, Gallice, Philippe, Durand, Philippe, Guichaoua, Marie-Roberte

المساهمون: Biogénotoxicologie et Mutagenèse Environnementale (BME), Université de la Méditerranée - Aix-Marseille 2, Centre National de la Recherche Scientifique (CNRS), Assistance Publique - Hôpitaux de Marseille (APHM), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), CNRS, INRA, Université Claude Bernard Lyon I, Ecole Normale supérieure de Lyon, Université de Méditerranée

المصدر: ISSN: 1096-6080.

مصطلحات موضوعية: spermatogenesis, rat, hexavalent chromium, in-vitro, chromosome, spermatocytes, quality, meiosis, culture, synaptonemal complex, synaptonemal complex damage, spermatogenic cells, sertoli-cells, germ-cells, population, exposure, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; There is evidence that exposure to environmental factors is at least partly responsible for changes in semen quality observed over the past decades. The detection of reproductive toxicants under Registration, Evaluation and Authorisation of Chemicals (REACH) will impact animal use for regulatory safety testing. We first validated a model of culture of rat seminiferous tubules for toxicological studies on spermatogenesis. Then, using this model of culture, we assessed the deleterious effects of 1, 10, and 100 μg/l hexavalent chromium [Cr(VI)] on meiotic cells. The prophase I of meiosis was studied in vivo and ex vivo. Bromo-2′-deoxyuridine (BrdU) was used to describe the kinetics of germ cell differentiation. SCP3 labeling allowed to establish the distribution of the stages of the meiotic prophase I and to perform a qualitative study of the pachytene stage in the absence or presence of Cr(VI). The development of the meiotic step of pubertal rats was similar in vivo and ex vivo. The number of total cells appeared not affected by the presence of Cr(VI) irrespective of its concentration. However, the numbers of late spermatocytes and of round spermatids were decreased by Cr(VI) even at the lower concentration. The percentage of synaptonemal complex abnormalities increased slightly with the time of culture and dramatically with Cr(VI) concentrations. This model of culture appears suitable for toxicological studies. This study shows that Cr(VI) is toxic for meiotic cells even at low concentrations, and its toxicity increases in a dose-dependent manner.

العلاقة: hal-02665346; https://hal.inrae.fr/hal-02665346Test; PRODINRA: 225899; WOS: 000279316500028

الإتاحة: https://doi.org/10.1093/toxsci/kfq099Test
https://hal.inrae.fr/hal-02665346Test

المؤلفون: Geoffroy-Siraudin, Cendrine, Perrard, Marie-Hélène, Chaspoul, Florence, Lanteaume, Andre, Gallice, Philippe, Durand, Philippe, Guichaoua, Marie-Roberte

المساهمون: Biogénotoxicologie et Mutagenèse Environnementale (BME), Université de la Méditerranée - Aix-Marseille 2, Centre National de la Recherche Scientifique (CNRS), Assistance Publique - Hôpitaux de Marseille (APHM), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), CNRS, INRA, Université Claude Bernard Lyon I, Ecole Normale supérieure de Lyon, Université de Méditerranée

المصدر: ISSN: 1096-6080.

مصطلحات موضوعية: spermatogenesis, rat, hexavalent chromium, in-vitro, chromosome, spermatocytes, quality, meiosis, culture, synaptonemal complex, synaptonemal complex damage, spermatogenic cells, sertoli-cells, germ-cells, population, exposure, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; There is evidence that exposure to environmental factors is at least partly responsible for changes in semen quality observed over the past decades. The detection of reproductive toxicants under Registration, Evaluation and Authorisation of Chemicals (REACH) will impact animal use for regulatory safety testing. We first validated a model of culture of rat seminiferous tubules for toxicological studies on spermatogenesis. Then, using this model of culture, we assessed the deleterious effects of 1, 10, and 100 μg/l hexavalent chromium [Cr(VI)] on meiotic cells. The prophase I of meiosis was studied in vivo and ex vivo. Bromo-2′-deoxyuridine (BrdU) was used to describe the kinetics of germ cell differentiation. SCP3 labeling allowed to establish the distribution of the stages of the meiotic prophase I and to perform a qualitative study of the pachytene stage in the absence or presence of Cr(VI). The development of the meiotic step of pubertal rats was similar in vivo and ex vivo. The number of total cells appeared not affected by the presence of Cr(VI) irrespective of its concentration. However, the numbers of late spermatocytes and of round spermatids were decreased by Cr(VI) even at the lower concentration. The percentage of synaptonemal complex abnormalities increased slightly with the time of culture and dramatically with Cr(VI) concentrations. This model of culture appears suitable for toxicological studies. This study shows that Cr(VI) is toxic for meiotic cells even at low concentrations, and its toxicity increases in a dose-dependent manner.

العلاقة: hal-02665346; https://hal.inrae.fr/hal-02665346Test; PRODINRA: 225899; WOS: 000279316500028

الإتاحة: https://doi.org/10.1093/toxsci/kfq099Test
https://hal.inrae.fr/hal-02665346Test

المؤلفون: Geoffroy-Siraudin, Cendrine, Perrard, Marie-Hélène, Chaspoul, Florence, Lanteaume, Andre, Gallice, Philippe, Durand, Philippe, Guichaoua, Marie-Roberte

المساهمون: Biogénotoxicologie et Mutagenèse Environnementale (BME), Université de la Méditerranée - Aix-Marseille 2, Centre National de la Recherche Scientifique (CNRS), Assistance Publique - Hôpitaux de Marseille (APHM), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), CNRS, INRA, Université Claude Bernard Lyon I, Ecole Normale supérieure de Lyon, Université de Méditerranée

المصدر: ISSN: 1096-6080.

مصطلحات موضوعية: spermatogenesis, rat, hexavalent chromium, in-vitro, chromosome, spermatocytes, quality, meiosis, culture, synaptonemal complex, synaptonemal complex damage, spermatogenic cells, sertoli-cells, germ-cells, population, exposure, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; There is evidence that exposure to environmental factors is at least partly responsible for changes in semen quality observed over the past decades. The detection of reproductive toxicants under Registration, Evaluation and Authorisation of Chemicals (REACH) will impact animal use for regulatory safety testing. We first validated a model of culture of rat seminiferous tubules for toxicological studies on spermatogenesis. Then, using this model of culture, we assessed the deleterious effects of 1, 10, and 100 μg/l hexavalent chromium [Cr(VI)] on meiotic cells. The prophase I of meiosis was studied in vivo and ex vivo. Bromo-2′-deoxyuridine (BrdU) was used to describe the kinetics of germ cell differentiation. SCP3 labeling allowed to establish the distribution of the stages of the meiotic prophase I and to perform a qualitative study of the pachytene stage in the absence or presence of Cr(VI). The development of the meiotic step of pubertal rats was similar in vivo and ex vivo. The number of total cells appeared not affected by the presence of Cr(VI) irrespective of its concentration. However, the numbers of late spermatocytes and of round spermatids were decreased by Cr(VI) even at the lower concentration. The percentage of synaptonemal complex abnormalities increased slightly with the time of culture and dramatically with Cr(VI) concentrations. This model of culture appears suitable for toxicological studies. This study shows that Cr(VI) is toxic for meiotic cells even at low concentrations, and its toxicity increases in a dose-dependent manner.

العلاقة: hal-02665346; https://hal.inrae.fr/hal-02665346Test; PRODINRA: 225899; WOS: 000279316500028

الإتاحة: https://doi.org/10.1093/toxsci/kfq099Test
https://hal.inrae.fr/hal-02665346Test

المؤلفون: Geoffroy-Siraudin, Cendrine, Perrard, Marie-Hélène, Chaspoul, Florence, Lanteaume, Andre, Gallice, Philippe, Durand, Philippe, Guichaoua, Marie-Roberte

المساهمون: Biogénotoxicologie et Mutagenèse Environnementale (BME), Université de la Méditerranée - Aix-Marseille 2, Centre National de la Recherche Scientifique (CNRS), Assistance Publique - Hôpitaux de Marseille (APHM), Institut de Génomique Fonctionnelle de Lyon (IGFL), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon), CNRS, INRA, Université Claude Bernard Lyon I, Ecole Normale supérieure de Lyon, Université de Méditerranée

المصدر: ISSN: 1096-6080.

مصطلحات موضوعية: spermatogenesis, rat, hexavalent chromium, in-vitro, chromosome, spermatocytes, quality, meiosis, culture, synaptonemal complex, synaptonemal complex damage, spermatogenic cells, sertoli-cells, germ-cells, population, exposure, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; There is evidence that exposure to environmental factors is at least partly responsible for changes in semen quality observed over the past decades. The detection of reproductive toxicants under Registration, Evaluation and Authorisation of Chemicals (REACH) will impact animal use for regulatory safety testing. We first validated a model of culture of rat seminiferous tubules for toxicological studies on spermatogenesis. Then, using this model of culture, we assessed the deleterious effects of 1, 10, and 100 μg/l hexavalent chromium [Cr(VI)] on meiotic cells. The prophase I of meiosis was studied in vivo and ex vivo. Bromo-2′-deoxyuridine (BrdU) was used to describe the kinetics of germ cell differentiation. SCP3 labeling allowed to establish the distribution of the stages of the meiotic prophase I and to perform a qualitative study of the pachytene stage in the absence or presence of Cr(VI). The development of the meiotic step of pubertal rats was similar in vivo and ex vivo. The number of total cells appeared not affected by the presence of Cr(VI) irrespective of its concentration. However, the numbers of late spermatocytes and of round spermatids were decreased by Cr(VI) even at the lower concentration. The percentage of synaptonemal complex abnormalities increased slightly with the time of culture and dramatically with Cr(VI) concentrations. This model of culture appears suitable for toxicological studies. This study shows that Cr(VI) is toxic for meiotic cells even at low concentrations, and its toxicity increases in a dose-dependent manner.

العلاقة: hal-02665346; https://hal.inrae.fr/hal-02665346Test; PRODINRA: 225899; WOS: 000279316500028

الإتاحة: https://doi.org/10.1093/toxsci/kfq099Test
https://hal.inrae.fr/hal-02665346Test