دورية أكاديمية
Serum Response Factor Is Essential for Prenatal Gastrointestinal Smooth Muscle Development and Maintenance of Differentiated Phenotype
| العنوان: | Serum Response Factor Is Essential for Prenatal Gastrointestinal Smooth Muscle Development and Maintenance of Differentiated Phenotype |
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| المؤلفون: | Park, Chanjae, Lee, Moon Y., Park, Paul J., Ha, Se E., Berent, Robyn M., Fuchs, Robert M., Miano, Joseph M., Becker, Laren S., Sanders, Kenton M., Ro, Seungil |
| المصدر: | https://doi.org/10.5056/jnm15063Test. |
| سنة النشر: | 2015 |
| المجموعة: | University of Nevada, Reno: ScholarWorks Repository |
| مصطلحات موضوعية: | Gastrointestinal tract, Myocyte, Platelet-derived growth factor receptor alpha, Rectal prolapse, Serum response factor, Smooth muscle cell |
| الوصف: | Smooth muscle cells (SMCs) characteristically express serum response factor (SRF), which regulates their development. The role of SRF in SMC plasticity in the pathophysiological conditions of gastrointestinal (GI) tract is less characterized. Methods We generated SMC-specific Sri knockout mice and characterized the prenatally lethal phenotype using ultrasound biomicroscopy and histological analysis. We used small bowel partial obstruction surgeries and primary cell culture using cell-specific enhanced green fluorescent protein (EGFP) mouse lines to study phenotypic and molecular changes of SMCs by immunofluorescence, Western blotting, and quantitative polymerase chain reaction. Finally we examined SRF change in human rectal prolapse tissue by immunofluorescence. Results Congenital SMC-specific Sri knockout mice died before birth and displayed severe GI and cardiac defects. Partial obstruction resulted in an overall increase in SRF protein expression. However, individual SMCs appeared to gradually lose SRF in the hypertrophic muscle. Cells expressing low levels of SRF also expressed low levels of platelet-derived growth factor receptor alpha (PDGFR alpha(low)) and Ki67. SMCs grown in culture recaptured the phenotypic switch from differentiated SMCs to proliferative PDGFR alpha(low) cells. The immediate and dramatic reduction of Srf and Myh11 mRNA expression confirmed the phenotypic change. Human rectal prolapse tissue also demonstrated significant loss of SRF expression. Conclusions SRF expression in SMCs is essential for prenatal development of the GI tract and heart. Following partial obstruction, SMCs down-regulate SRF to transition into proliferative PDGFR alpha(low) cells that may represent a phenotype responsible for their plasticity. These findings demonstrate that SRF also plays a critical role in the remodeling process following GI injury. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | |
| اللغة: | unknown |
| تدمد: | 2093-0879 |
| العلاقة: | Park, C., Lee, M. Y., Park, P. J., Ha, S. E., Berent, R. M., Fuchs, R., … Ro, S. (2015). Serum Response Factor Is Essential for Prenatal Gastrointestinal Smooth Muscle Development and Maintenance of Differentiated Phenotype. Journal of Neurogastroenterology and Motility, 21(4), 589–602. doi:10.5056/jnm15063; http://hdl.handle.net/11714/5980Test |
| DOI: | 10.5056/jnm15063 |
| الإتاحة: | https://doi.org/10.5056/jnm15063Test http://hdl.handle.net/11714/5980Test |
| حقوق: | Creative Commons Attribution-NonCommercial 4.0 International ; http://creativecommons.org/licenses/by-nc/4.0Test ; The Korean Society of Neurogastroenterology and Motility. |
| رقم الانضمام: | edsbas.A9BC65C6 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20930879 |
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| DOI: | 10.5056/jnm15063 |