دورية أكاديمية
Cloning, purification and structure determination of the HIV integrase-binding domain of lens epithelium-derived growth factor.
| العنوان: | Cloning, purification and structure determination of the HIV integrase-binding domain of lens epithelium-derived growth factor. |
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| المؤلفون: | Hannon, C, Cruz-Migoni, A, Platonova, O, Owen, RL, Nettleship, JE, Miller, A, Carr, SB, Harris, G, Rabbitts, TH, Phillips, SEV |
| المساهمون: | Rabbitts, Terence |
| بيانات النشر: | INT UNION CRYSTALLOGRAPHY |
| سنة النشر: | 2020 |
| المجموعة: | The Institute of Cancer Research (ICR): Publications Repository |
| مصطلحات موضوعية: | Humans, HIV Integrase, Adaptor Proteins, Signal Transducing, Transcription Factors, Crystallization, Crystallography, X-Ray, Amino Acid Sequence, Catalytic Domain, Protein Conformation, Models, Molecular |
| الوصف: | Lens epithelium-derived growth factor (LEDGF)/p75 is the dominant binding partner of HIV-1 integrase in human cells. The crystal structure of the HIV integrase-binding domain (IBD) of LEDGF has been determined in the absence of ligand. IBD was overexpressed in Escherichia coli, purified and crystallized by sitting-drop vapour diffusion. X-ray diffraction data were collected at Diamond Light Source to a resolution of 2.05 Å. The crystals belonged to space group P21, with eight polypeptide chains in the asymmetric unit arranged as an unusual octamer composed of four domain-swapped IBD dimers. IBD exists as a mixture of monomers and dimers in concentrated solutions, but the dimers are unlikely to be biologically relevant. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | Print-Electronic; 149; application/pdf |
| اللغة: | English |
| تدمد: | 2053-230X |
| العلاقة: | Acta crystallographica. Section F, Structural biology communications, 2018, 74 (Pt 3), pp. 143 - 149; https://repository.icr.ac.uk/handle/internal/4261Test |
| DOI: | 10.1107/s2053230x18001553 |
| الإتاحة: | https://doi.org/10.1107/s2053230x18001553Test https://repository.icr.ac.uk/handle/internal/4261Test |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: | edsbas.A143EA24 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 2053230X |
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| DOI: | 10.1107/s2053230x18001553 |